Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,579,791	(A)7→6	coding (79/132 nt)	U712_18380 →	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,579,785	0	A	.	97.5%	339.5 / 1.2	79	coding (73/132 nt)	U712_18380	Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (51/26);  total (52/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

GAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTAT  >  CP006881/3579649‑3579918                                                                                                                                         |                                                                                                                                      gAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAACCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                      >  7:59047/1‑138 (MQ=255)    gcaagcaTACAATAGACATCTCTAGTAGAGGAAATAAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaa                                                                                                                                  <  2:119503/139‑1 (MQ=255)      aagcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                <  6:158494/139‑3 (MQ=255)      aagcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTACGGGTGTCTGCATGCaaaaaaca                                                                                                                                >  3:35812/1‑137 (MQ=255)      aagcaTACAATAGACATCTCTAGTAGAGGAAACCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                <  8:59047/139‑3 (MQ=255)             cAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCgg                                                                                                                        <  7:332369/139‑1 (MQ=255)             cAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCgg                                                                                                                        <  5:174109/139‑1 (MQ=255)               aTAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCTAAACCGCACTTTTTATTTCTGGTGTCTGCATGC‑AAAAAACACACCCGGAt                                                                                                                      >  5:52253/1‑139 (MQ=255)               aTAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGAt                                                                                                                      >  2:246923/1‑139 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCTCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                  >  4:39128/1‑139 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                  >  4:54685/1‑139 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                                  >  6:204057/1‑139 (MQ=255)                      tctcTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTg                                                                                                                >  3:246646/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGTTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  8:70159/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  3:37970/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  4:100711/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  2:332418/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  4:155264/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  6:197470/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  2:34407/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  1:98349/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                              >  1:38628/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACACGGATCGTCTGGa                                                                                                              >  5:36360/1‑138 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGCAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGaaaa                                                                                                           >  8:77014/1‑139 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTgg                                                                                                               <  3:9673/135‑1 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGaaaa                                                                                                           >  5:105361/1‑139 (MQ=255)                            gtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtc                                                                                                         >  5:196055/1‑139 (MQ=255)                            gtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtc                                                                                                         >  6:23452/1‑139 (MQ=255)                            gtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATAAAAAAAACGGATGAAGTCGGAATCAATCCCAAACCGCACATTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTTGaaaaa                                                                                                          >  6:180080/1‑137 (MQ=255)                             tagAGGAAATCAACAAAGAAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCTGAATCAATCAGAAAACGCACTTTTTATTTCTGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtct                                                                                                        >  5:267752/1‑139 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                >  3:147317/1‑112 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                <  4:147317/114‑3 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                       <  3:100711/138‑1 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                       >  2:50957/1‑138 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                       >  1:278261/1‑138 (MQ=255)                                agGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                     <  5:65199/139‑1 (MQ=255)                                 ggAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAACCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGAGCGTCTGGAAAATCTcaac                                                                                                    >  2:140909/1‑139 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  3:739/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  1:321367/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  1:204515/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  5:238156/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  6:312364/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGACTGGAAAATCTcaac                                                                                                    >  1:179684/1‑138 (MQ=255)                                  gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACAGCACTTTTTATTTCGTGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  6:204280/1‑138 (MQ=255)                                    aaTCAACAAAGCAACGCTGTTACACAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTATTTATTTCGGGTGTCTCCATGC‑AAAAAACACACCCGGATCGTCTGTAAAATCTCAACAAt                                                                                                 >  1:186211/1‑139 (MQ=255)                                      tCAACAAAGCAACGCTGTTACACAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCACTCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAATAACACACCCGTATCGTCTGGAAAATCTCAACAATcc                                                                                               <  6:58502/139‑1 (MQ=255)                                          caaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCggg                                                                                            >  7:271474/1‑139 (MQ=255)                                            aaGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTg                                                                                          >  1:128177/1‑138 (MQ=255)                                                  cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                                   >  7:183006/1‑139 (MQ=255)                                                  cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                                   >  2:202139/1‑139 (MQ=255)                                                   gCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtctt                                                                                  <  7:234846/139‑1 (MQ=255)                                                    cTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtctt                                                                                  <  8:271474/139‑1 (MQ=255)                                                       ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    <  6:131860/117‑1 (MQ=255)                                                       ttACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                    >  5:131860/1‑117 (MQ=255)                                                               aaaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                      >  8:254721/1‑139 (MQ=255)                                                               aaaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                      >  1:266677/1‑139 (MQ=255)                                                                 aTGAATATGAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGAATGC‑AAAAAACACACCCGGATCGTCTGGAATATCTCAAAAATCCGGGTGCAGATCTTGTTTAATACAttgt                                                                    >  5:209036/1‑137 (MQ=255)                                                                      tatCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGc                                                               <  8:77770/139‑1 (MQ=255)                                                                        tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATTCATTTTTTTGCgt                                                             <  1:10958/139‑1 (MQ=255)                                                                        tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                             <  1:34407/139‑1 (MQ=255)                                                                        tCAAAAAAAAGGATGAAGTCGGAATCAATCCGAAACAGCACTTTTTATTTCTGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                             <  4:35812/139‑1 (MQ=255)                                                                         cAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                             >  1:278334/1‑138 (MQ=255)                                                                         cAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                             <  2:278334/138‑1 (MQ=255)                                                                           aaaaaaCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTTCgtctg                                                          >  8:313403/1‑139 (MQ=255)                                                                                   gATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCt                                                   >  1:27924/1‑138 (MQ=255)                                                                                            ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                         <  8:28114/139‑1 (MQ=255)                                                                                                   atcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACACGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaac                                   >  2:231263/1‑138 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACaaaa                               <  4:739/139‑1 (MQ=255)                                                                                                        aaaCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                             >  3:326153/1‑139 (MQ=255)                                                                                                           ccGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCg                          <  5:204057/139‑1 (MQ=255)                                                                                                             gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                        <  3:155264/139‑1 (MQ=255)                                                                                                             gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                        <  3:54685/139‑1 (MQ=255)                                                                                                             gCACTTTTTATTTCGGGGGTCTGCATGC‑AAAAACCACACCCGGATCGTGTGGTAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                        <  3:1968/139‑1 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                         <  5:108214/121‑1 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaaaa                                         >  6:108214/1‑121 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                       >  2:5047/1‑139 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                       >  6:183612/1‑139 (MQ=255)                                                                                                                   tttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCa                   >  5:326415/1‑138 (MQ=255)                                                                                                                    ttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCaa                  <  3:106998/138‑1 (MQ=255)                                                                                                                        ttCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAgg              <  6:196055/138‑1 (MQ=255)                                                                                                                          cGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGct            <  1:246923/138‑1 (MQ=255)                                                                                                                           gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAAtt                                       >  1:236338/1‑110 (MQ=255)                                                                                                                           gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAAtt                                       <  2:236338/110‑1 (MQ=255)                                                                                                                           gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGctct          >  4:125103/1‑139 (MQ=255)                                                                                                                           gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGctct          <  4:326153/139‑1 (MQ=255)                                                                                                                            ggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCtt         <  6:318122/139‑1 (MQ=255)                                                                                                                                    catgc‑aAAACACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAGCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTAt  <  6:36360/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTAT  >  CP006881/3579649‑3579918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑