BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,186	(T)_8→9	intergenic (–/‑792)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,178	1	.	T	92.9%	7693.0 / inf	2257	intergenic (–/‑800)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base . (69/90); new base T (883/1209); total (954/1303)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2289 total aligned reads displayed.

ATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑C‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAG  >  pSIJ663_pHT315_araE_xy/2040‑2313

aTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑c                                                                                                                                          >  6:2838/1‑139 (MQ=255)

                tgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAg                                                                                                                            >  5:7817/1‑137 (MQ=255)

                tgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGcc                                                                                                                          >  4:7698/1‑139 (MQ=255)

                tgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGcc                                                                                                                          >  5:12179/1‑139 (MQ=255)

                     cTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCa                                                                                                                      <  6:196316/138‑1 (MQ=255)

                          cGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAAttt                                                                                                                 <  7:227725/138‑1 (MQ=255)

                            tGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGGCAAGTTATATGTTTCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCATATTtctc                                                                                                              >  2:48497/1‑139 (MQ=255)

                                 tataAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑C‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCAt                                                                                                        >  4:306868/1‑139 (MQ=255)

                                       tCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  1:220926/139‑1 (MQ=255)

                                       tCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  1:264477/139‑1 (MQ=255)

                                       tCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   <  2:251595/139‑1 (MQ=255)

                                         cGGGAAACATTTTTACATCTTTAGAAGTGCAAGTAAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTTAAACAACAGTTCGTTTATTTACAAT‑CTTTTTTTTTATTAGCAGTCATATTACTCCCTAATCTCGtct                                                                                                 >  8:55156/1‑139 (MQ=255)

                                         cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACATT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGtct                                                                                                 >  4:537/1‑139 (MQ=255)

                                          gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGtctc                                                                                                >  5:24103/1‑139 (MQ=255)

                                          gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGtctc                                                                                                >  1:145262/1‑139 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  4:203075/1‑135 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  5:200172/1‑139 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  8:32841/1‑139 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  2:126484/1‑139 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTctct                                                                                               >  4:104025/1‑139 (MQ=255)

                                           ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑C‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                              >  7:259907/1‑139 (MQ=255)

                                                         aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  3:125683/139‑1 (MQ=255)

                                                         aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  3:105007/139‑1 (MQ=255)

                                                         aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  8:124703/139‑1 (MQ=255)

                                                          tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 >  7:30529/1‑138 (MQ=255)

                                                           cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCga                                                                                                   <  2:74520/119‑2 (MQ=255)

                                                           cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttta                                                                               >  7:151338/1‑139 (MQ=255)

                                                             ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTcc                                                                                                             >  6:210137/1‑107 (MQ=255)

                                                             ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattttat                                                                              >  1:312406/1‑138 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  6:54670/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  4:278087/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  3:20532/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  7:109097/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  3:332782/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  3:146406/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  7:49872/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTATATATCTTTATTTTATCATAAAgg                                                                      <  7:325824/139‑1 (MQ=255)

                                                                    tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGGTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  2:232603/139‑1 (MQ=255)

                                                                     gCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAg                                                                       >  2:100117/1‑137 (MQ=255)

                                                                      ctagtaaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCAACAACAGTTTGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                                                    <  2:182735/137‑1 (MQ=255)

                                                                      caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAAtt                                                                                                                  <  3:261721/93‑1 (MQ=255)

                                                                      caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTTTTTTATCATTAAGGAg                                                                    >  5:39098/1‑139 (MQ=255)

                                                                      caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  1:62628/137‑1 (MQ=255)

                                                                       aagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                                                   >  6:105236/1‑139 (MQ=255)

                                                                               ttATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                                           <  6:336946/139‑1 (MQ=255)

                                                                                    tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                      >  4:181438/1‑139 (MQ=255)

                                                                                      tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                    <  2:149750/139‑1 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                   >  8:224584/1‑90 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtt                                                                                      >  5:155662/1‑103 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTTTATTATGATTAAGGGGTAGTTTTACCGTcgcggg                                                  >  1:237968/1‑137 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                          <  1:77359/115‑1 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATTAAGGAGTATTTGAACCGTcgcgcg                                                  >  1:37794/1‑139 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      <  5:312728/119‑1 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgcg                                                  >  7:17808/1‑139 (MQ=255)

                                                                                        tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCGCTTTATATCTTTTTTTTATCATAAAGGAGTTTTTGAACCTTcgcgcg                                                  >  6:237773/1‑139 (MQ=255)

                                                                                         cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTta                                                                                            >  1:28915/1‑96 (MQ=255)

                                                                                         cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                      >  2:280806/1‑118 (MQ=255)

                                                                                         cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                 >  1:117124/1‑139 (MQ=255)

                                                                                           ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                      <  8:151991/132‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  6:127444/104‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  3:282000/138‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  6:76849/138‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               >  1:173211/1‑138 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  8:25638/138‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  8:276212/138‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  7:203292/139‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  6:155287/139‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  4:94120/139‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAACCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  4:149857/139‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑C‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  7:70324/137‑1 (MQ=255)

                                                                                            taATGATTTGTGGAAGTTTTGCCACAACAGGTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  5:221126/139‑1 (MQ=255)

                                                                                            taATGAGTTGTGGAAGTTTTGCCACAACAGTTGGATTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                              <  6:258826/139‑1 (MQ=255)

                                                                                              aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  3:308634/136‑1 (MQ=255)

                                                                                                    tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTTTATCTTTATTTTATCATAAAGGAGTATTTGAACCGTAGCGCGGGACAGGTTTAt                                      >  5:62919/1‑139 (MQ=255)

                                                                                                    tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  4:253019/96‑1 (MQ=255)

                                                                                                    tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               >  3:80122/1‑130 (MQ=255)

                                                                                                    tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               >  5:288296/1‑130 (MQ=255)

                                                                                                     gtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTta                                                                                            <  4:327664/84‑1 (MQ=255)

                                                                                                      tgtaaGTTTTGCCACAACAGTTGGTTTATTTACTAT‑CTTTTTTTTTATTATCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    <  3:62155/136‑1 (MQ=255)

                                                                                                      tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                 <  5:175625/94‑1 (MQ=255)

                                                                                                      tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                    >  2:206203/1‑139 (MQ=255)

                                                                                                       ggtaGTTTTGACTCAACAGTTGGTTTATTTAAAAT‑CTTTTTTTTTATTATCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGATCCGTCGCGCGGGACAGGTTTATGa                                    <  8:8199/135‑1 (MQ=255)

                                                                                                        gAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGTGACAGGTTTTTGAt                                   <  7:132170/138‑1 (MQ=255)

                                                                                                        gAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                   <  6:307750/138‑1 (MQ=255)

                                                                                                        gAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCAGTCAAATTTCTCACTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                   <  5:263167/138‑1 (MQ=255)

                                                                                                         aaGTTTTGCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                 <  4:227844/139‑1 (MQ=255)

                                                                                                                gCCACAACAGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATa                                                                          <  7:88630/91‑1 (MQ=255)

                                                                                                                     aacaGTTGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                               <  2:319610/113‑1 (MQ=255)

                                                                                                                          ttGGTTTATTTACAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                    <  3:235664/103‑1 (MQ=255)

                                                                                                                            ggTTTATTTACAAT‑CTTTTTTTTTATTAGGCGTCTAATTTCTCCCTGATCTCGTCTCTTTATATCTTTATTTTATCATATAGGAGTATTTGAACCGTGGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataa               <  2:29713/138‑1 (MQ=255)

                                                                                                                                tatttaCAATAT‑TTTTTTTTATTAGCCGTCAAATTTCTACCTCTTCTAGTCTCTTTATATCTTTATTTTATCATAAATTATTTTTTTAACATTCGCGAGTGTAAGGTTTATGATATTGATTTTTTATTGATTAATTGa           <  8:18179/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  8:206475/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  1:202938/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  4:125533/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  8:102700/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  8:298944/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  6:175468/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  1:100117/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  3:179352/138‑1 (MQ=255)

                                                                                                                                tatttaCAAT‑CTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTATCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGGCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa           <  7:275312/138‑1 (MQ=255)

ATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAT‑C‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAG  >  pSIJ663_pHT315_araE_xy/2040‑2313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`