Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	28,183	C→T	A457V (GCC→GTC)	U712_00100 →	DNA polymerase III subunit gamma/tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	28,183	0	C	T	100.0%	137.3 / NA	42	A457V (GCC→GTC)	U712_00100	DNA polymerase III subunit gamma/tau
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

TCCAATTACAAAGCGCCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGCCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTCCGGACTAATCTTGAGCAGATTT  >  CP006881/28050‑28314                                                                                                                                      |                                                                                                                                     tCCACTTACACAGCCCAAGAAGGCAGAATTCATGATATTTTGACGGACGCCACAAGCCCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCGTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCtt                                                                                                                                 <  4:252102/139‑1 (MQ=255)     aTTACAAAGCGCCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCt                                                                                                                             >  6:227258/1‑139 (MQ=255)     aTTACAAAGCGCCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCt                                                                                                                             >  6:231881/1‑139 (MQ=255)            aGCGCCAGTTGGCAGAATTCATTAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGAc                                                                                                                      <  1:255452/139‑1 (MQ=255)            aGCGCCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGATGGGGCAAGCTTCTTGCTCATCTCAACCAGCAAAACAAAGTATCACATTTCTATTTGCTGAATGa                                                                                                                       >  6:199324/1‑138 (MQ=255)             gcgcCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACa                                                                                                                     <  8:217413/139‑1 (MQ=255)                           aaTTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCtt                                                                                                                                 >  1:46769/1‑113 (MQ=255)                           aaTTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCtt                                                                                                                                 <  2:46769/113‑1 (MQ=255)                           aaTTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCt                                                                                                       >  1:243380/1‑139 (MQ=255)                             ttCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAAAAGTTGGGGCACGCTTCTTGCTCATCTCAAACAGCAAAACAGAGTATCACATGTCGCTATGCTCAATGACAGTGAACCTGTGGCTGc                                                                                                     >  4:45876/1‑139 (MQ=255)                                     aTTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATcgg                                                                                             >  8:184331/1‑139 (MQ=255)                                       tttGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATcggc‑g                                                                                          >  5:55283/1‑139 (MQ=255)                                                           cGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCaa                                                                      <  1:48480/139‑1 (MQ=255)                                                             gATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCaaa                                                                     >  3:303341/1‑138 (MQ=255)                                                                         cTCAGACACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACACAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCa                                                          <  3:45876/137‑1 (MQ=255)                                                                         cTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCCATTTTTCTGAAAATAAAAAAAGAAATTCaat                                                        >  6:40346/1‑137 (MQ=255)                                                                         cTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCa                                                          <  6:55283/137‑1 (MQ=255)                                                                         cTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCAtt                                                        >  8:40720/1‑139 (MQ=255)                                                                         cTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCAGGATCGGC‑GGCATTTGTTCTGAAATTCAAATAAGAAATTCaat                                                        >  1:97249/1‑137 (MQ=255)                                                                                   gTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGCGGGCTTTTGTTATAAAATTCAAATTTGAAATACTTTTTAAATTgt                                               >  2:45867/1‑138 (MQ=255)                                                                                   gTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGt                                              >  6:9520/1‑139 (MQ=255)                                                                                       gggCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          <  3:25125/139‑1 (MQ=255)                                                                                       gggCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          <  7:184331/139‑1 (MQ=255)                                                                                       gggCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          <  5:59597/139‑1 (MQ=255)                                                                                       gggCAAGCTTCTTGCTCATCTAAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          <  7:61863/139‑1 (MQ=255)                                                                                          cAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAg                                       <  2:243380/139‑1 (MQ=255)                                                                                          cAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAg                                       <  5:51645/139‑1 (MQ=255)                                                                                          cAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAg                                       <  1:45867/139‑1 (MQ=255)                                                                                            aGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGa                                     <  4:303341/139‑1 (MQ=255)                                                                                                        aTCTCAAACAGCAAAACAAATTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTAATTGTAAAATGGTCGCTGAGGATAACAACGGAGt                         >  8:99393/1‑139 (MQ=255)                                                                                                        aTCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          <  2:185837/122‑1 (MQ=255)                                                                                                        aTCTCAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCt                                          >  1:185837/1‑122 (MQ=255)                                                                                                          ctcAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCg                                            >  4:210897/1‑118 (MQ=255)                                                                                                          ctcAAACAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCg                                            <  3:210897/118‑1 (MQ=255)                                                                                                             aaaCAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTc                        <  5:199324/135‑1 (MQ=255)                                                                                                             aaaCAGCAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTc                        <  4:8594/135‑1 (MQ=255)                                                                                                                   cAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCa                                                          >  4:55155/1‑95 (MQ=255)                                                                                                                   cAAAACAAAGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCa                                                          <  3:55155/95‑1 (MQ=255)                                                                                                                    aaaaCAAAGTATCACATGTCGATTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGGTATGAAATTCAAATATGAAATTCATTCTAAAATGGTCGCAGAGGATAACACCGCCGTCCGGCCTAATCt             >  1:108326/1‑139 (MQ=255)                                                                                                                           aGTATCACATGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTCCGGACTAATCTTGAg         >  3:153791/1‑136 (MQ=255)                                                                                                                                  caTGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACTGAGTCCGGACTAATCTTGAGCAGAttt  >  7:76276/1‑136 (MQ=255)                                                                                                                                  caTGTCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTCCGGACTAATCTTGAGCAGAttt  >  2:241478/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                     TCCAATTACAAAGCGCCAGTAGGCAGAATTCATGAGATTTTGAAGGAAGCCACAAGACCGGATCTTGATCTGCTCAGAAACAGTTGGGGCAAGCTTCTTGCTCATCTCAAACAGCAAAACAAAGTATCACATGCCGCTTTGCTGAATGACAGTGAACCTGTGGCTGCCGGATCGGC‑GGCATTTGTTCTGAAATTCAAATATGAAATTCATTGTAAAATGGTCGCTGAGGATAACAACGGAGTCCGGACTAATCTTGAGCAGATTT  >  CP006881/28050‑28314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑