Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,082	A→C	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	133.4 / NA	39	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/24);  total (15/24)

GGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAGC  >  CP006881/3724962‑3725215                                                                                                                         |                                                                                                                                       ggcggcTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                                                                                      <  5:176074/139‑1 (MQ=255)      cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                                 <  4:281139/139‑1 (MQ=255)                               aTTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCaaaa                                                                                          <  6:239992/137‑1 (MQ=255)                                ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                       <  7:249574/139‑1 (MQ=255)                                ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                       <  3:10273/139‑1 (MQ=255)                                ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                       <  5:82602/139‑1 (MQ=255)                                       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                <  2:43261/139‑1 (MQ=255)                                       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                <  7:21499/139‑1 (MQ=255)                                         aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                                                                              <  1:304579/139‑1 (MQ=255)                                          tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCacac                                                                             <  6:66000/139‑1 (MQ=255)                                                    aTAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAt                                                                   <  5:148320/139‑1 (MQ=255)                                                        aacgaaacgaGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACaa                                                               <  7:163902/139‑1 (MQ=255)                                                                             tGCACAGCCGCCTTTCTTTGGGATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCCGTTTTTGTGTCCGGCGTCACCCAGTTCATCAAAAAGCCCAGCCACCCCCGTAAAGATACAAGCAAACGTTTAATAGaaaaa                                           >  6:177728/1‑138 (MQ=255)                                                                                      gcctttCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCg                                 <  7:246024/136‑1 (MQ=255)                                                                                       cctttCTTTGGTATGTACGGTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGGTCATCAAAAAGACCATCCCCACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACCTCCcgccgc                                >  8:246024/3‑139 (MQ=255)                                                                                       ccttgcTTTGGTATGTACGCTGAGCCGAAAAGACCGCGGCAGCCGCAGTGTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGc                                <  3:269164/134‑1 (MQ=255)                                                                                         tttCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTATGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCCCCCCCGTAAAGATACAAGCAAACCTTTCATAGAAAAACAACCCTccccgt                              >  4:338233/1‑135 (MQ=255)                                                                                            cattGGTATGTAAGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTATGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAAACCTCCGcttcgt                           >  7:3537/3‑137 (MQ=255)                                                                                                 gTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                        <  5:282379/89‑1 (MQ=255)                                                                                                 gTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                        <  5:287215/89‑1 (MQ=255)                                                                                                 gTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                        >  6:287215/1‑89 (MQ=255)                                                                                                 gTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                                                        >  6:282379/1‑89 (MQ=255)                                                                                                    tGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCAGGCGTCACCCAGTAAATCAAAAAGCCCATCCACACCCGTAAAGATACAAGCAAACGATTCATAGCAAAACATCCCTCCGCTTCTTATTtgcag                   >  3:104088/1‑139 (MQ=255)                                                                                                      tACGCTGCGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCGCCGCGTTCATCATACAGCCCATCAACACCCGTAAAGATTCCAGCAAACGTTTCATCGAACGACATCCCTCCGCTGCTTGTTggcaggc                 >  6:128286/1‑139 (MQ=255)                                                                                                      tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc                 <  1:312758/139‑1 (MQ=255)                                                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca                <  1:102308/138‑1 (MQ=255)                                                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca                <  5:177728/138‑1 (MQ=255)                                                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca                <  3:40019/138‑1 (MQ=255)                                                                                                           tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCATCTTTTTTGCAGGCAGcat            >  3:129841/1‑137 (MQ=255)                                                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                >  7:195716/1‑70 (MQ=255)                                                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                <  8:195716/70‑1 (MQ=255)                                                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCACACGTTTCATAGAAAAACATCCCTCCGCTGCTTTTTGGCAGGCCGCC‑tt          >  8:140912/1‑139 (MQ=255)                                                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCGGTCCGGCGTCACCCAGTTCATCAAACAGACCATCCCCCCCCGTAAAGCTACAAGCACCCGGTTCCCCTCAAATCCTCCCTCCGCGTCTGTGTGGCCGGCCCCC‑tt          >  6:31496/1‑139 (MQ=255)                                                                                                              gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                       >  6:272473/1‑61 (MQ=255)                                                                                                              gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                       <  5:272473/61‑1 (MQ=255)                                                                                                                  aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                <  6:17545/64‑1 (MQ=255)                                                                                                                  aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                                >  5:17545/1‑64 (MQ=255)                                                                                                                  aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAGCC‑tttttagc    >  3:291314/1‑135 (MQ=255)                                                                                                                      gACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTagcagc  <  5:46065/138‑1 (MQ=255)                                                                                                                         |                                                                                                                                       GGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAGC  >  CP006881/3724962‑3725215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑