| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,237 | A→G | intergenic (‑69/‑460) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,237 | 0 | A | G | 87.1% | 72.8 / ‑3.9 | 32 | intergenic (‑69/‑460) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (22/5); minor base C (1/3); total (23/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT > CP006881/3725119‑3725369 | ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 3:160165/1‑139 (MQ=255)ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTGTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGGTTTCACACCCCCAGCCCCCAAGAAAAGACGGCCGCtt > 3:227820/1‑139 (MQ=255)ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTTGCAGGCCGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACCGCCGAtg > 1:131064/1‑138 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCATCCGCAGCCCGCAAGAAAAGACGGCCGAgttcgtt > 7:305466/1‑139 (MQ=255) cccGTAACTATACAACCAAACGTTACATCAAAAAACATCCCTCGGTTTATTTTTGGCCGGAAGCTT‑T‑TTTAGCAGCCTTGTTTCTCTGCCGCCGCCCCCATAAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc < 4:324146/139‑1 (MQ=255) cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCAAAATAGCGGctc > 7:290085/1‑139 (MQ=255) cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc > 5:61976/1‑139 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCGGctc > 4:232437/1‑137 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTGTCGCAAACTAGCGGCGCTGGCAAAAc > 1:8230/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTACTTTTCTCGCAAACTAGCGGCTCTACCaaaa > 2:207806/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 7:82277/1‑139 (MQ=255) aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGTCCGATTTCTTTTGTCGCAACCTAACGTCTCGAGCAAAACt > 8:254030/1‑138 (MQ=255) gaaaaCTTTTAATAGAAAAAAATCCCTCCGCTTCTTTTTGGATGGCAGCTT‑T‑TTTAGCCGCCGGTTTTCTCACCCGCAGCCCTAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 8:305466/137‑1 (MQ=255) ccacaCGTTTAATATAAAAACATCCCGCCGATGATTTTTGGCCGCCCGCTT‑T‑TTTACCCCCCGGTTTTCTCCCCCCCCGCCCCCAACAAACGACGGCCGATTTCTTTTCTTGAAAACTAGCGGCTCTAGCAAAACTCAt < 8:3009/135‑1 (MQ=255) aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 7:188931/139‑1 (MQ=255) cggTTTCATACAAAACAGACCGTCCGCTGTGTTTTGGCCGGCCGC‑C‑TGTTTCGCCGCGCGTTTCCTCAGCCGCCGCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTCGCGGCTCTAGCAAAACTCATGAct < 8:34469/137‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 3:338233/139‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTTGCAAACAAGCGGCTCGAGCAAAACTCATGAct > 7:3009/1‑139 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 4:148668/1‑139 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTGCTTTTGGGCAGGCACC‑CTT‑TTTCGCAGCCCGTTTTCTCAGCCGCAGCCCCCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:197917/139‑1 (MQ=255) aaaaaCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGAAAGAc > 5:139164/1‑139 (MQ=255) tCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGa > 5:84248/1‑138 (MQ=255) cTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAATCTATGAAAGACCGTTGaaa > 3:62698/1‑137 (MQ=255) cGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCACCCGCCCCCCGAAACAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGaa < 7:73015/139‑1 (MQ=255) cttTTTGCCCGGCAGCTT‑T‑TTTAGCAGCCGGTTTTCACAGCCCCACCCCGCAACAAACCACGGCCTATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt < 6:61976/138‑1 (MQ=255) ttttGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTg > 7:250034/1‑139 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCATTGTCACAATATGAGTGAAAAAACCaa > 5:60398/1‑139 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTCGCAAAATATCGGCTCTAGCCAAACTCAAGACTCTATGAAAGACCGTGGAAAGAGAATTATTTGTCACAATATTAGTGAAAAAACCaa > 2:267940/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTTAAAAAACCaa > 5:92890/1‑138 (MQ=255) ttttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGAGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAACAACAAGt > 1:23518/1‑139 (MQ=255) ccGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTGTCGCAAACTAGCGGCTCTAGCCAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATTAGTGCAAAAACCCACAAGTTTTTATAAg > 6:186156/1‑139 (MQ=255) cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGATTGAAAAAACCAACAAGTTTTTTGaatgtt > 4:3541/1‑135 (MQ=255) | TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT > CP006881/3725119‑3725369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |