Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,091	A→T	intergenic (‑205/‑96)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,091	0	A	T	100.0%	301.0 / NA	86	intergenic (‑205/‑96)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/59);  total (27/59)

TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑TTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGC  >  CP006881/3722957‑3723224                                                                                                                                          |                                                                                                                                      tttttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATataa                                                                                                                                     >  6:159675/1‑138 (MQ=255)   tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                                                  >  6:234778/1‑137 (MQ=255)   tttCATTTACGCAAATTCTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                                                  >  7:76585/1‑137 (MQ=255)          tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGAc                                                                                                                            >  2:24342/1‑138 (MQ=255)                             gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                                                           <  3:244761/136‑3 (MQ=255)                             gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                                        >  8:198915/1‑137 (MQ=255)                             gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                                        >  2:122349/1‑137 (MQ=255)                                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                                                    >  1:117622/1‑138 (MQ=255)                                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                                                    <  6:117170/138‑1 (MQ=255)                                     tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATATTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                                                                  <  2:148757/137‑1 (MQ=255)                                     tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                 <  2:301506/138‑1 (MQ=255)                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  1:306311/138‑3 (MQ=255)                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  4:137789/138‑3 (MQ=255)                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  3:318780/138‑3 (MQ=255)                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  6:171663/138‑3 (MQ=255)                                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  7:241852/138‑3 (MQ=255)                                          gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                 >  6:280697/1‑133 (MQ=255)                                          gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                 <  5:280697/133‑1 (MQ=255)                                           tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          <  7:142841/139‑1 (MQ=255)                                           tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          <  1:308749/139‑1 (MQ=255)                                           tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          <  2:295300/139‑1 (MQ=255)                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          >  4:81762/1‑138 (MQ=255)                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          >  7:223347/1‑138 (MQ=255)                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                          >  2:168799/1‑138 (MQ=255)                                            cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATAATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGATATACTTca                                                                                          >  1:324814/1‑138 (MQ=255)                                             acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                                        <  2:303674/139‑1 (MQ=255)                                                ataccGCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACATCACAata                                                                                     <  3:246592/136‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  2:18132/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  4:50349/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  4:47069/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  5:234778/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  5:253177/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  1:267597/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  3:81762/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  1:63753/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  8:76585/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  1:180011/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  7:210675/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  1:205279/139‑1 (MQ=255)                                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  2:255730/139‑1 (MQ=255)                                                 tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                                                    <  5:335358/139‑1 (MQ=255)                                                  cccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                   <  8:87893/139‑1 (MQ=255)                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                   <  8:251275/138‑1 (MQ=255)                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                   <  7:198915/138‑1 (MQ=255)                                                   ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                   <  1:122349/138‑1 (MQ=255)                                                    cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                                                                                 <  2:210153/139‑1 (MQ=255)                                                     gCTTCTTCAGATTAAGAAAACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  7:262297/139‑1 (MQ=255)                                                     gCTTATTCATATTAAGAATACGCTTTAATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAAAATTTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                >  5:128695/1‑139 (MQ=255)                                                     gCTTATTCAGATTAAGAATACTCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  5:231136/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                         >  7:294249/1‑113 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                                         <  8:294249/114‑2 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     >  3:43261/1‑134 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  4:43261/134‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  7:176055/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  1:24342/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  1:201336/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  8:40618/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  1:113704/139‑1 (MQ=255)                                                     gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  6:279594/139‑1 (MQ=255)                                                      cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  3:264380/133‑1 (MQ=255)                                                      cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     >  4:264380/1‑133 (MQ=255)                                                       ttATTCAGATTTAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  2:295805/137‑1 (MQ=255)                                                         aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTAAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     >  6:1267/1‑130 (MQ=255)                                                         aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTAAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                     <  5:1267/130‑1 (MQ=255)                                                          ttCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTTAAATACTTCAAAATAAAGTGCtat                                                                            >  6:231136/1‑138 (MQ=255)                                                           tCAGATTTAGAATACGCTTTCATCCTAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATCTCATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCTCAATATCGTGCtata                                                                           <  6:216866/138‑1 (MQ=255)                                                               aTTAAGAATACGCTTTCGTCATAAATCATGATAGCGTTTTCGTCAACT‑A‑TTTTTGTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGAAATACTTCACAAGATCGTGCTATACTTTg                                                                      >  8:180542/1‑139 (MQ=255)                                                               aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               >  6:296494/1‑112 (MQ=255)                                                               aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                               <  5:296494/114‑3 (MQ=255)                                                               aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                      >  1:143074/1‑139 (MQ=255)                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                >  6:269499/1‑119 (MQ=255)                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                >  3:147644/1‑119 (MQ=255)                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  4:147644/119‑1 (MQ=255)                                                                         cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                <  5:269499/119‑1 (MQ=255)                                                                              tcatcaTAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaag                                                        >  4:7243/1‑138 (MQ=255)                                                                                 tcatAAATCATGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                    <  1:331002/139‑1 (MQ=255)                                                                                          aTGATAGCGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaag                                           <  5:159675/139‑2 (MQ=255)                                                                                                 cGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                     >  2:140986/1‑138 (MQ=255)                                                                                                 cGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                     >  7:324626/1‑138 (MQ=255)                                                                                                 cGTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg                                     >  4:87950/1‑138 (MQ=255)                                                                                                  gTTTTCGTCAACTAT‑TTTTT‑TAGTTAAATAGTTTGATATAACATGTATAAAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATTCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa                                   <  6:128695/139‑1 (MQ=255)                                                                                                        gTCAACT‑ATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTc                              <  6:179311/138‑1 (MQ=255)                                                                                                                   tttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                    <  6:259686/138‑1 (MQ=255)                                                                                                                     tt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                  <  2:143074/138‑1 (MQ=255)                                                                                                                         aGTTAAATAGTTTGATATAACATGTAGACAAAAATTAGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaaagg               <  1:168799/138‑3 (MQ=255)                                                                                                                              aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa          <  8:223347/138‑1 (MQ=255)                                                                                                                                     tGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGc  >  7:84383/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                      TTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑G‑TTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGC  >  CP006881/3722957‑3723224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑