BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,039	C→T	intergenic (–/‑939)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,039	0	C	T	98.1%	7185.5 / 65.4	2206	intergenic (–/‑939)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (25/16); new base T (1204/958); total (1229/977)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

Only 100 of 2241 total aligned reads displayed.

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

cTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                      <  2:221057/139‑1 (MQ=255)

      tCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAc                                                                                                                                <  1:168860/139‑1 (MQ=255)

          aCTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAgg                                                                                                                            <  2:100161/139‑1 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCATTTCAACGCTATTAGGTTCTTTAGGATTTTTCCCGACATAGTCGGGGTGTGCCACGATTTCGTTTATGTGCGATGAATATTTTTTATAAAAACAAGGATGGTGTTTCt                                                                                                                 >  8:289545/1‑139 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAg                                                                                                                             <  2:123026/127‑1 (MQ=255)

                     aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCt                                                                                                                 >  3:36589/1‑139 (MQ=255)

                      ttAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCtt                                                                                                                >  7:201234/1‑139 (MQ=255)

                         gTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTAc                                                                                                             <  2:84568/139‑1 (MQ=255)

                          tataTTGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACg                                                                                                            <  3:128376/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  1:57837/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          >  7:181162/1‑139 (MQ=255)

                             atGTTCAGTTAAAATCTTTACCAATTCAACTCTATTATGTTGGGTAGGATTTTGACAGACATAGTCGGGTTGTTCAACGATAGCTTTTATGTGCGATTAATATTTTTTTTAAATACCAGGAGGTTGTTTCTTTACTTg                                                                                                          >  4:261838/1‑138 (MQ=255)

                              tGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  7:114800/137‑1 (MQ=255)

                               ggTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCtt                                                                                                       >  3:87383/1‑139 (MQ=255)

                                gTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCtt                                                                                                       >  4:89978/1‑138 (MQ=255)

                                   aTTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGTTGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtat                                                                                                    >  5:185750/1‑138 (MQ=255)

                                   aTTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtat                                                                                                    >  3:152815/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     <  1:19680/104‑1 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAc                                                                                                                                >  4:124941/1‑109 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  1:318791/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  7:24901/1‑138 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATATTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  5:310926/1‑138 (MQ=255)

                                               tACCACATCAACGCTATTAGGTTCTTTAGGATTTTTCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGa                                                                                          <  5:275396/136‑1 (MQ=255)

                                               tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        >  5:144912/1‑138 (MQ=255)

                                                     ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGc                                                                                                         <  7:51207/115‑1 (MQ=255)

                                                     ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACAATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  2:65916/1‑138 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:128919/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  8:263023/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  6:145996/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  2:19636/138‑1 (MQ=255)

                                                         tcGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACAt                                                                             <  3:271177/138‑1 (MQ=255)

                                                             tATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  6:271937/1‑138 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               >  1:101026/1‑131 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  7:323545/1‑136 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  7:130747/1‑136 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  1:80550/1‑136 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                       <  8:156412/139‑1 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCACCGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTAAGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:258826/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGCATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  4:237198/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaaa                                                                                                                                   <  2:36396/71‑1 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATgttgtt                                                                                                                    <  3:203164/86‑1 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACg                                                                                                            <  7:78710/94‑1 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:58586/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:89495/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  4:193077/1‑137 (MQ=255)

                                                                       tttAGGATTTTGACCGACATAGTCGGGGTTTTGAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCTGGATGTGTTTTCTTTACGTGCTTTATTATTCCGGGAAACATTTTTAGATCGTTAGTTGTGcat                                                               >  6:74651/1‑138 (MQ=255)

                                                                         tAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  6:334264/81‑1 (MQ=255)

                                                                         tAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  3:320037/94‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:269799/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  2:260385/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  5:166731/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  8:128943/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTAGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  2:149529/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  5:20733/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  6:193404/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  5:34396/138‑1 (MQ=255)

                                                                             aTTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaag                                                         <  6:246388/139‑1 (MQ=255)

                                                                              ttttGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaagt                                                        <  7:221918/139‑1 (MQ=255)

                                                                                ttGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGtt                                                       <  8:244261/138‑1 (MQ=255)

                                                                                ttGCCCGACATAGTCGGGGAGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGGTGTATCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGtt                                                       <  6:173471/138‑1 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATgttgtt                                                                                                                    >  4:33802/1‑76 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  6:302299/131‑1 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  8:19451/1‑138 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  1:269465/1‑138 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  4:64482/1‑138 (MQ=255)

                                                                                   cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  2:301086/138‑1 (MQ=255)

                                                                                   cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTatat                                                   <  3:29090/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  7:294381/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  1:227677/139‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  6:222591/139‑1 (MQ=255)

                                                                                       aCATAGTCGGTGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  3:68386/139‑1 (MQ=255)

                                                                                       aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  3:222400/139‑1 (MQ=255)

                                                                                       aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  6:56374/139‑1 (MQ=255)

                                                                                       aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  2:277071/139‑1 (MQ=255)

                                                                                             tCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          >  1:152419/1‑74 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  6:118161/1‑139 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  2:237651/1‑139 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAAAATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  3:240976/1‑139 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  4:2812/93‑1 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgt                                 >  8:219379/1‑139 (MQ=255)

                                                                                                     gtCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  4:215065/122‑1 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                >  8:79763/1‑138 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         >  3:179401/1‑139 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               >  4:16839/1‑83 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  1:290412/1‑101 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  7:162321/1‑139 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  5:237151/1‑139 (MQ=255)

                                                                                                                    tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 >  8:179248/1‑108 (MQ=255)

                                                                                                                        tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg              <  4:147512/139‑1 (MQ=255)

                                                                                                                          cGATGTATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTTTCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            <  4:311442/139‑1 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  6:93907/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  1:249606/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  8:56238/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  8:31792/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  2:167482/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  8:107854/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  5:116814/1‑139 (MQ=255)

                                                                                                                           gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  3:104950/89‑1 (MQ=255)

                                                                                                                           gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  4:104950/1‑89 (MQ=255)

                                                                                                                           gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTg           >  8:332408/1‑139 (MQ=255)

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`