Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,662,478	T→G	I388S (ATC→AGC)	U712_08545 →	putative flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,662,478	0	T	G	100.0%	248.0 / NA	73	I388S (ATC→AGC)	U712_08545	putative flagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/36);  total (37/36)

AATGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAATCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGAC  >  CP006881/1662348‑1662608                                                                                                                                   |                                                                                                                                   aaTGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGa                                                                                                                            >  8:98200/1‑139 (MQ=255) aaTGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGa                                                                                                                            >  1:146957/1‑139 (MQ=255) aaTGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGa                                                                                                                            >  4:280809/1‑139 (MQ=255) aaTGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGa                                                                                                                            >  2:189451/1‑139 (MQ=255)   tGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGAc                                                                                                                           >  4:80136/1‑138 (MQ=255)   tGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAACCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGAc                                                                                                                           >  7:35643/1‑138 (MQ=255)        aaaCAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATg                                                                                                                      >  4:286249/1‑138 (MQ=255)        aaaCAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATg                                                                                                                      >  7:314413/1‑138 (MQ=255)        aaaCAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATg                                                                                                                      >  2:236161/1‑138 (MQ=255)               aaCGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGa                                                                                                              <  4:117487/139‑1 (MQ=255)               aaCGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGa                                                                                                              <  8:35643/139‑1 (MQ=255)                     ggCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGAGAAATGAAAATGGAATGttt                                                                                                        <  3:225221/139‑1 (MQ=255)                     ggCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        <  5:73176/139‑1 (MQ=255)                     ggCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        >  6:163421/1‑139 (MQ=255)                     ggCTGATCAAGTGATTAATGCGGTGAAGGAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        <  6:244005/139‑1 (MQ=255)                      gCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        <  5:279842/138‑1 (MQ=255)                      gCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        >  7:253088/1‑138 (MQ=255)                           tCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGt                                                                                                  <  5:48045/139‑1 (MQ=255)                                tGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAAt                                                                                             >  7:164532/1‑139 (MQ=255)                                   ttAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCa                                                                                           <  2:146957/138‑1 (MQ=255)                                   ttAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTGCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCAt                                                                                          <  2:301257/139‑1 (MQ=255)                                     aaTGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAg                                                                                        >  6:38794/1‑139 (MQ=255)                                         cGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATc                                                                                    >  1:41958/1‑139 (MQ=255)                                           tGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGt                                                                                                  >  7:298039/1‑123 (MQ=255)                                           tGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGt                                                                                                  <  8:298039/123‑1 (MQ=255)                                            ggAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAg                                                                                 >  8:220534/1‑139 (MQ=255)                                                gCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCaa                                                                             <  8:183002/139‑1 (MQ=255)                                                            taCGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGaa                                                                 <  5:163421/139‑1 (MQ=255)                                                              cGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAAtt                                                               >  2:147201/1‑139 (MQ=255)                                                              cGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAAtt                                                               >  7:295069/1‑139 (MQ=255)                                                                   ttCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCtt                                                           >  8:38251/1‑138 (MQ=255)                                                                   ttCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCtt                                                           >  5:296784/1‑138 (MQ=255)                                                                     cGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGTAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        <  2:139521/139‑1 (MQ=255)                                                                      ggCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGGATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        <  8:259299/138‑1 (MQ=255)                                                                      ggCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        <  7:91394/138‑1 (MQ=255)                                                                       gCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACa                                                      <  8:164532/139‑1 (MQ=255)                                                                          gATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCa                                                   <  3:299455/139‑1 (MQ=255)                                                                           aTCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCAt                                                  >  5:121830/1‑139 (MQ=255)                                                                           aTCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCAt                                                  >  3:331358/1‑139 (MQ=255)                                                                           aTCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCAt                                                  >  3:232440/1‑139 (MQ=255)                                                                               aagggAAGGTTCACTATTCGGCTGAACCCTGAACATTTATGATTTGTCACAAGCAAGCAGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTc                                              <  4:308881/137‑1 (MQ=255)                                                                               aCGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATTGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTc                                              <  5:230979/139‑1 (MQ=255)                                                                                 gggAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCt                                            <  7:38251/139‑1 (MQ=255)                                                                                     aGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGc                                        >  1:59463/1‑139 (MQ=255)                                                                                           aCTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGc                                  <  6:296784/139‑1 (MQ=255)                                                                                                 cGGCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCAt                            >  8:113244/1‑139 (MQ=255)                                                                                                   gCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          <  5:38794/139‑1 (MQ=255)                                                                                                   gCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          <  3:286249/139‑1 (MQ=255)                                                                                                   gCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          <  8:253088/139‑1 (MQ=255)                                                                                                   gCTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          <  8:314413/139‑1 (MQ=255)                                                                                                    cTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        <  6:171094/60‑1 (MQ=255)                                                                                                    cTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGttt                                                                                                        >  5:171094/1‑60 (MQ=255)                                                                                                    cTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          <  5:178445/138‑1 (MQ=255)                                                                                                    cTGAACCCTGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTg                          >  6:178445/1‑138 (MQ=255)                                                                                                            tGAACATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATg                 >  5:116473/1‑139 (MQ=255)                                                                                                              aaCATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATg                 <  1:147201/137‑1 (MQ=255)                                                                                                              aaCATTTAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGc               >  5:162048/1‑139 (MQ=255)                                                                                                                   ttAGGATTTGTTACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCa                                                   <  1:43386/98‑1 (MQ=255)                                                                                                                   ttAGGATTTGTTACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCa                                                   >  2:43386/1‑98 (MQ=255)                                                                                                                   ttAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTc           >  2:222624/1‑138 (MQ=255)                                                                                                                   ttAGGATTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTc           >  4:74084/1‑138 (MQ=255)                                                                                                                        aTTTGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAAtt     <  2:65633/139‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGTAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATAGCAGAATATGGCCGTCCAAATTgcc  >  7:304387/1‑137 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGTATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGa   <  3:74084/138‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        <  2:278571/85‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        >  7:58291/1‑85 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        >  1:278571/1‑85 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGaa                                                        <  8:58291/85‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAg                                         <  2:47897/100‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAg                                         >  1:47897/1‑100 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGa   <  7:60150/138‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGa   <  1:236161/138‑1 (MQ=255)                                                                                                                           tGTCACAAGCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGAc  >  7:318216/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   AATGCCGAAACAAAAACGGTGGCTGATCAAGTGATTAATGCGTGGAAGCAGATGAAATATACGCCTTTCGGCAGATCGACGGGAAGTTTCACTATTCGGCTGAACCCTGAACATTTAGGATTTGTCACAATCAAGCTGACAAATGAAAATGGAATGTTTCAGAGTAAAATCATAGCATCTAGCCAATCAGCAAAAGAATTGCTTGAACAGCATCTTCCTCAGCTGAAGCAATCATTGCCGAATATGGCCGTCCAAATTGAC  >  CP006881/1662348‑1662608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑