Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,101	A→C	L242L (CTT→CTG)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,101	0	A	C	100.0%	379.0 / NA	100	L242L (CTT→CTG)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/55);  total (45/55)

GAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGAT  >  CP006881/3723969‑3724231                                                                                                                                     |                                                                                                                                   gagaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGca                                                                                                                              <  4:6031/139‑1 (MQ=255)           ccTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATa                                                                                                                      <  6:8000/137‑1 (MQ=255)           ccTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAgg                                                                                                                    >  8:12432/1‑139 (MQ=255)             tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc                                                                                                                   >  2:83216/1‑138 (MQ=255)             tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc                                                                                                                   >  2:57793/1‑138 (MQ=255)              gCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGct                                                                                                                  >  6:93320/1‑137 (MQ=255)              gCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcg                                                                                                                  >  1:249894/1‑137 (MQ=255)              gCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGAACATTGATAGGcg                                                                                                                  >  5:41446/1‑137 (MQ=255)               cAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcga                                                                                                                 <  1:4102/138‑3 (MQ=255)                   tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa                                                                                                            >  4:290745/1‑139 (MQ=255)                   tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa                                                                                                            >  4:268645/1‑139 (MQ=255)                       aGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACt                                                                                                        >  4:288456/1‑139 (MQ=255)                            ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                                   >  5:174486/1‑139 (MQ=255)                            ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                                   >  3:55342/1‑139 (MQ=255)                            ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                                   >  3:123197/1‑139 (MQ=255)                            ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                                   >  1:148125/1‑139 (MQ=255)                               ttCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTgg                                                                                                <  6:132828/139‑1 (MQ=255)                               ttCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTgg                                                                                                <  8:192262/139‑1 (MQ=255)                               ttCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTgg                                                                                                <  1:19962/139‑1 (MQ=255)                                 cctagagaTCTTGAAGACGCTGGTCATGCAGCGGGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  6:174486/135‑1 (MQ=255)                                 ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  8:137462/138‑1 (MQ=255)                                 ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  8:95835/138‑1 (MQ=255)                                 ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  3:268645/138‑1 (MQ=255)                                 ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  2:90842/138‑1 (MQ=255)                                  cGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGAt                                                                                              <  7:48099/138‑1 (MQ=255)                                    agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               >  8:230667/1‑135 (MQ=255)                                    agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  7:230667/135‑1 (MQ=255)                                    agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGa                                                                                            >  2:261467/1‑138 (MQ=255)                                    agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGa                                                                                            >  5:303808/1‑138 (MQ=255)                                    agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTAAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGa                                                                                            >  1:49703/1‑138 (MQ=255)                                      agaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAgg                                                                                                                    >  6:113892/1‑112 (MQ=255)                                      agaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAgg                                                                                                                    <  5:113892/112‑1 (MQ=255)                                       gaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                                        <  1:57793/139‑1 (MQ=255)                                       gaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                                        <  1:83216/139‑1 (MQ=255)                                        aTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                                        >  3:90731/1‑138 (MQ=255)                                          cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGaaa                                                                                          >  4:50224/1‑134 (MQ=255)                                          cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGaaa                                                                                          <  3:50224/134‑1 (MQ=255)                                             gAAGCCGCTGGACATGCAGCGGGATATAGCAGGCCTCTGCTGCCGGCCAATGTAATTGAAATTCAGTTGGCAAAAACTCCGCCAGCTCCAGCGCACATTGCTATGAGAATGAATACTTTTTTTGGATGAAATACAGc                                                                                    <  5:44598/137‑1 (MQ=255)                                             gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  7:12432/137‑1 (MQ=255)                                             gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  7:68661/137‑1 (MQ=255)                                             gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCa                                                                                   <  2:39286/138‑1 (MQ=255)                                                gACGCTGGTCATGCAGCGTGATATAGCCGGCCTCGGAATCCGGCAAATGTAATTGATTTTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                               <  6:89295/139‑1 (MQ=255)                                                gACGCTGGACATGCAGCGTTATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                               <  2:148125/139‑1 (MQ=255)                                                gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                               <  1:48449/139‑1 (MQ=255)                                                gACGCTGGACATGCAGCGTGATAAAGCCGGCCTCGGATTCCCGCAAATGTAATTCATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATATGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                               <  7:153093/139‑1 (MQ=255)                                                  cGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcct                                                                             <  8:271466/139‑1 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               >  1:210286/1‑120 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  2:210286/120‑1 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            >  2:208820/1‑139 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            >  6:110805/1‑139 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            >  5:296232/1‑139 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            >  8:169113/1‑139 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            <  6:303808/139‑1 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            <  6:336897/139‑1 (MQ=255)                                                   gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            <  4:90731/139‑1 (MQ=255)                                                     tGGACATGCTGCGTGATATAGCCGGGCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                                          <  8:123101/139‑1 (MQ=255)                                                       gACATGCATCTTGATATAGCAGGCCTAGGATTCAGGCAATTGTAATTGATATTGATTTTTCAAAAACTCAGGCAGCTCCAGCTCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTcc                                                                        <  5:93320/139‑1 (MQ=255)                                                       gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                                          <  2:249894/137‑1 (MQ=255)                                                       gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                                          <  1:261467/137‑1 (MQ=255)                                                       gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTcc                                                                        <  4:37568/139‑1 (MQ=255)                                                       gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTc                                                                         >  2:221922/1‑138 (MQ=255)                                                        aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa                                                                       <  5:110805/139‑1 (MQ=255)                                                          aTGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATa                                                                     <  1:208820/139‑1 (MQ=255)                                                            gCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcc                                                                              <  6:268386/128‑1 (MQ=255)                                                            gCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCAGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcc                                                                              >  5:268386/1‑128 (MQ=255)                                                             cAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGa                                                                  >  6:56425/1‑139 (MQ=255)                                                             cAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAGTGATAGGCGAATGAATTCTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGa                                                                  >  8:166799/1‑139 (MQ=255)                                                                 gTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATg                                                                >  6:246518/1‑137 (MQ=255)                                                                  tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                             <  3:288456/139‑1 (MQ=255)                                                                  tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                             >  1:55352/1‑139 (MQ=255)                                                                    atatAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  5:276353/114‑1 (MQ=255)                                                                    atatAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTACAGCGCACATTGATAGGCGAATGAATACTTTTTTTTGATGAAATAAAGc                                                                                    >  6:276353/1‑114 (MQ=255)                                                                          ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               <  3:78779/97‑1 (MQ=255)                                                                          ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                               >  4:78779/1‑97 (MQ=255)                                                                          ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAt                                                     >  5:215633/1‑139 (MQ=255)                                                                           cGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTATTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAtt                                                    <  7:204072/139‑1 (MQ=255)                                                                            ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  1:318633/106‑1 (MQ=255)                                                                            ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    >  2:318633/1‑106 (MQ=255)                                                                             gCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                             >  7:241272/1‑128 (MQ=255)                                                                             gCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                             <  8:241272/128‑1 (MQ=255)                                                                             gCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAttt                                                   >  4:251451/1‑138 (MQ=255)                                                                               cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  1:59739/103‑1 (MQ=255)                                                                               cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    >  2:59739/1‑103 (MQ=255)                                                                                  ggATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTcc                                             <  4:55342/139‑1 (MQ=255)                                                                                       ccGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCg                                        >  3:322785/1‑139 (MQ=255)                                                                                        cGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    <  5:125656/94‑1 (MQ=255)                                                                                        cGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc                                                                                    >  6:125656/1‑94 (MQ=255)                                                                                        cGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            <  5:137360/102‑1 (MQ=255)                                                                                        cGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                            >  6:137360/1‑102 (MQ=255)                                                                                        cGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCgg                                       >  2:76787/1‑138 (MQ=255)                                                                                                 tAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACAATGGAGCGCTTCATTTGATTCCAACCGGCTGACcgcg                              <  4:312281/139‑1 (MQ=255)                                                                                                    ttGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAt                           >  3:305229/1‑139 (MQ=255)                                                                                                        tatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAtcgc                        <  3:290745/138‑3 (MQ=255)                                                                                                          ttCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCaaa                     >  8:30271/1‑139 (MQ=255)                                                                                                            catttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAAt                    <  4:14535/135‑1 (MQ=255)                                                                                                            catttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAAt                    <  8:202971/135‑1 (MQ=255)                                                                                                                    aaaaaCTCCGCCCGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCGCGTCCATACGATGGAGCGCTTGATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa            <  7:316617/138‑1 (MQ=255)                                                                                                                    aaaaaCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa            <  5:246518/138‑1 (MQ=255)                                                                                                                            cgCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGTATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGAACGCGTATCGCAACTGAGTGACAACGCGTTGa   >  5:302264/2‑139 (MQ=255)                                                                                                                             gCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAt  <  8:287911/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   GAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGAT  >  CP006881/3723969‑3724231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑