BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,045	T→C	intergenic (‑159/‑142)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,045	0	T	C	98.1%	379.6 / NA	103	intergenic (‑159/‑142)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (36/65); minor base A (2/0); total (38/65)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAG  >  CP006881/3722922‑3723174

tAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCAGCATAAATAATGATAGCGTTTTCg                                                                                                                      >  5:216866/1‑139 (MQ=255)

   aaTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACGATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCa                                                                                                                   <  2:282856/139‑1 (MQ=255)

       cGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACta                                                                                                               >  3:280756/1‑138 (MQ=255)

       cGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACta                                                                                                               >  1:255730/1‑138 (MQ=255)

                     tgACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                <  2:310924/138‑1 (MQ=255)

                     ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                <  4:19602/139‑1 (MQ=255)

                      gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                >  2:180011/1‑138 (MQ=255)

                      gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTaaa                                                                                                >  2:267597/1‑138 (MQ=255)

                        cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAg                                                                                             >  3:95383/1‑139 (MQ=255)

                        cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAg                                                                                             >  6:335358/1‑139 (MQ=255)

                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                            >  2:331002/1‑139 (MQ=255)

                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                            <  3:20742/139‑1 (MQ=255)

                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                            <  2:254008/139‑1 (MQ=255)

                         acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGt                                                                                            <  3:301026/139‑1 (MQ=255)

                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                          <  6:254245/139‑1 (MQ=255)

                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                          <  6:222633/139‑1 (MQ=255)

                           aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGttt                                                                                          >  8:241852/1‑139 (MQ=255)

                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATat                                                                                     <  4:95383/139‑2 (MQ=255)

                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATat                                                                                     <  4:289846/139‑2 (MQ=255)

                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                      >  8:176055/1‑138 (MQ=255)

                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                      >  2:306311/1‑138 (MQ=255)

                                tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATa                                                                                      >  7:251275/1‑138 (MQ=255)

                                   tttttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATataa                                                                                   >  6:159675/1‑138 (MQ=255)

                                     tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                >  6:234778/1‑137 (MQ=255)

                                     tttCATTTACGCAAATTCTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAcat                                                                                >  7:76585/1‑137 (MQ=255)

                                            tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGAc                                                                          >  2:24342/1‑138 (MQ=255)

                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTa                                                                                                  <  4:67013/97‑1 (MQ=255)

                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTa                                                                                                  >  3:67013/1‑97 (MQ=255)

                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTa                                                                                                  >  5:274742/1‑97 (MQ=255)

                                                             ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTa                                                                                                  <  6:274742/97‑1 (MQ=255)

                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                         <  3:244761/136‑3 (MQ=255)

                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                      >  8:198915/1‑137 (MQ=255)

                                                               gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                      >  2:122349/1‑137 (MQ=255)

                                                                    cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                  <  6:117170/138‑1 (MQ=255)

                                                                    cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                  >  1:117622/1‑138 (MQ=255)

                                                                       tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATATTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                <  2:148757/137‑1 (MQ=255)

                                                                       tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                               <  2:301506/138‑1 (MQ=255)

                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  7:241852/138‑3 (MQ=255)

                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  3:318780/138‑3 (MQ=255)

                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  6:171663/138‑3 (MQ=255)

                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  4:137789/138‑3 (MQ=255)

                                                                         ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  1:306311/138‑3 (MQ=255)

                                                                            gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                               >  6:280697/1‑133 (MQ=255)

                                                                            gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                               <  5:280697/133‑1 (MQ=255)

                                                                             tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        <  2:295300/139‑1 (MQ=255)

                                                                             tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        <  7:142841/139‑1 (MQ=255)

                                                                             tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        <  1:308749/139‑1 (MQ=255)

                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        >  7:223347/1‑138 (MQ=255)

                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        >  4:81762/1‑138 (MQ=255)

                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                        >  2:168799/1‑138 (MQ=255)

                                                                              cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATAATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGATATACTTca                                        >  1:324814/1‑138 (MQ=255)

                                                                               acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                      <  2:303674/139‑1 (MQ=255)

                                                                                  ataccGCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACATCACAata                                   <  3:246592/136‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  8:76585/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  5:234778/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  5:253177/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  7:210675/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  3:81762/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:18132/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:63753/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  2:255730/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:267597/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:205279/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  1:180011/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  4:50349/139‑1 (MQ=255)

                                                                                  aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  4:47069/139‑1 (MQ=255)

                                                                                     ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  8:251275/138‑1 (MQ=255)

                                                                                     ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  7:198915/138‑1 (MQ=255)

                                                                                     ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                 <  1:122349/138‑1 (MQ=255)

                                                                                      cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                               <  2:210153/139‑1 (MQ=255)

                                                                                       gCTTCTTCAGATTAAGAAAACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  7:262297/139‑1 (MQ=255)

                                                                                       gCTTATTCATATTAAGAATACGCTTTAATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAAAATTTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              >  5:128695/1‑139 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACTCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  5:231136/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                       >  7:294249/1‑113 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                       <  8:294249/114‑2 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  3:43261/1‑134 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  4:43261/134‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  1:201336/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  1:113704/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  1:24342/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  7:176055/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  8:40618/139‑1 (MQ=255)

                                                                                       gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  6:279594/139‑1 (MQ=255)

                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  4:264380/1‑133 (MQ=255)

                                                                                        cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  3:264380/133‑1 (MQ=255)

                                                                                         ttATTCAGATTTAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  2:295805/137‑1 (MQ=255)

                                                                                           aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTAAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   <  5:1267/130‑1 (MQ=255)

                                                                                           aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTAAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                   >  6:1267/1‑130 (MQ=255)

                                                                                            ttCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTTAAATACTTCAAAATAAAGTGCtat                          >  6:231136/1‑138 (MQ=255)

                                                                                             tCAGATTTAGAATACGCTTTCATCCTAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATCTCATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCTCAATATCGTGCtata                         <  6:216866/138‑1 (MQ=255)

                                                                                                 aTTAAGAATACGCTTTCGTCATAAATCATGATAGCGTTTTCGTCAACT‑ATTTTTGTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGAAATACTTCACAAGATCGTGCTATACTTTg                    >  8:180542/1‑139 (MQ=255)

                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             <  5:296494/114‑3 (MQ=255)

                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                             >  6:296494/1‑112 (MQ=255)

                                                                                                 aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                    >  1:143074/1‑139 (MQ=255)

                                                                                                           cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              >  3:147644/1‑119 (MQ=255)

                                                                                                           cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  4:147644/119‑1 (MQ=255)

                                                                                                           cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              >  6:269499/1‑119 (MQ=255)

                                                                                                           cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                              <  5:269499/119‑1 (MQ=255)

                                                                                                                tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaag      >  4:7243/1‑138 (MQ=255)

                                                                                                                   tcatAAATCATGATAGCGTTTTCGTCAACTATTTTTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag  <  1:331002/139‑1 (MQ=255)

TAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAG  >  CP006881/3722922‑3723174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`