Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,082	A→C	A29A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,082	0	A	C	100.0%	123.5 / NA	34	A29A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/22);  total (12/22)

CTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTT  >  CP006881/3724967‑3725209                                                                                                                    |                                                                                                                                cTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                          <  1:174103/139‑1 (MQ=255) cTTTCGCCTTCAGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                          <  1:292766/139‑1 (MQ=255)                tttCTACCACATTCACCGCCACCCCCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGt                                                                                            <  3:20143/138‑1 (MQ=255)                  tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                                                          <  5:3623/138‑1 (MQ=255)                                  ccATCCGCCATTCATAAAACGAATCGAGTCCTGCGGCCTGCACAGCCGCCTTTCATTGGTATGTACGCGGAGCCGAATAGACCGCGGCAGCCGCAGGTTTTCTGTCCGGCGTCACGCAGTTCATCAAAAAGACCATCca                                                                         <  3:141654/139‑1 (MQ=255)                                  ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                         <  4:71878/139‑1 (MQ=255)                                  ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                         <  1:246315/139‑1 (MQ=255)                                  ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                         <  3:45594/139‑1 (MQ=255)                                    aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCa                                                                                                  <  8:22698/112‑1 (MQ=255)                                    aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCa                                                                                                  >  7:22698/1‑112 (MQ=255)                                           tttCATAAACCTAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTTGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTaa                                                                <  1:362444/137‑1 (MQ=255)                                                                    ggCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa                                       <  4:43982/139‑1 (MQ=255)                                                                        tGCACAGCCGCCGTTCTTTGGTATGTACGCTGAGCCGAATAGCCCGCGGCACCCGCCGTTTTTGGGTCCGGCGTCGCCCAGTTGCTCAAAAAGACCCTCCCCACCCGTAAAGATACAAGCCAACGTTTGATAGTAAAAc                                   >  1:37583/1‑139 (MQ=255)                                                                          cacaGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAt                                 >  6:361274/1‑139 (MQ=255)                                                                               ccgccgttCGTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTGTGGCCGGCCTCCCCCAGGTCCTCAAAAAGCCGATCCACACCCGGAAAGATACAAGCAAACGTTTGATAGAAAAACATCCCTc                            >  5:123266/7‑139 (MQ=255)                                                                               ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                         <  3:162424/94‑1 (MQ=255)                                                                               ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTATGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                         >  4:162424/1‑94 (MQ=255)                                                                                    tttCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTGACCCAGTTCATAAAAAACACCATCCACACCCGTAAATATACAAGCAAACGTTTCATAGAAAAACATCCcgccgcgt                       >  2:37736/1‑137 (MQ=255)                                                                                       cTTTGGTATGTACGCTGAGCCTAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGcttct                     <  1:356330/138‑1 (MQ=255)                                                                                       cTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGATCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGcttct                     <  3:29854/138‑1 (MQ=255)                                                                                         ttGGTATGTACGCTGAGCCGCATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTGCATCAAAAAGACCATCCACACCCCTAAAGATTCACGCAAACGTTTCATAGAAAACCATCCCTCCGCTTGtttt                  >  5:289653/1‑139 (MQ=255)                                                                                         ttGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa                                       >  2:128759/1‑118 (MQ=255)                                                                                         ttGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaa                                       <  1:128759/118‑1 (MQ=255)                                                                                                 tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc          <  1:173736/139‑1 (MQ=255)                                                                                                   cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca         <  7:195025/138‑1 (MQ=255)                                                                                                   cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca         <  3:50697/138‑1 (MQ=255)                                                                                                   cGCTGAGCCGAATAGACCGAGGCAGCCGCAGTTTTTCTGTCCTGAGTCACCCAGTTCATCAAAAAGACCATCCACACACGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca         <  2:130979/138‑1 (MQ=255)                                                                                                      tGAGCCGAATAGCCCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGTTACAAGCAACCGTTTCATAGAAACACATCCCTCCGCGTGGTTGTGGCCGGCCGCCt     >  4:250851/1‑139 (MQ=255)                                                                                                      tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACACGTAAAGATAAAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGccg     >  1:130979/1‑138 (MQ=255)                                                                                                         gCCGAATAGCCCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTACATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACTTTTCATACAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCtttt  <  4:96090/139‑1 (MQ=255)                                                                                                         gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                          >  7:127889/1‑35 (MQ=255)                                                                                                         gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                                                          <  8:127889/35‑1 (MQ=255)                                                                                                         gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAt                                 <  5:341979/108‑1 (MQ=255)                                                                                                         gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAt                                 >  6:341979/1‑108 (MQ=255)                                                                                                                    |                                                                                                                                CTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTT  >  CP006881/3724967‑3725209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑