BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,039	C→T	intergenic (–/‑939)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,039	0	C	T	99.3%	15063.5 / 34.5	4585	intergenic (–/‑939)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (16/15); new base T (2492/2048); total (2521/2064)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 4672 total aligned reads displayed.

GCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1902‑2173

gtttGGTTCCAACTTTATAGCAATTAGAAAATGGACTTTTAAAATC‑TTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGAAATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                      <  5:191391/137‑1 (MQ=255)

 cTTGGGTCGAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                      <  3:321808/139‑1 (MQ=255)

 cTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                      <  6:190406/139‑1 (MQ=255)

       tCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAc                                                                                                                                <  4:92111/139‑1 (MQ=255)

         cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTATGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCa                                                                                                                              <  2:83009/139‑1 (MQ=255)

         cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     <  7:359954/132‑1 (MQ=255)

               tataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgt                                                                                                                        >  5:280773/1‑139 (MQ=255)

                ataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       >  4:40108/1‑139 (MQ=255)

                ataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCAGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       >  3:91208/1‑139 (MQ=255)

                 taGCAATTAGTATATGGTCATTTAAAATCTTTACGAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  2:153248/138‑1 (MQ=255)

                 taGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  7:119538/138‑1 (MQ=255)

                      aTTAGTATATGGTCATTTAAATTCTTTACCAATTCAACGCTATTAGGTTGTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAACAGGATGTTGTTTCt                                                                                                                 >  5:61713/1‑139 (MQ=255)

                      aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     <  5:86804/119‑1 (MQ=255)

                      aTTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                    <  2:368890/120‑1 (MQ=255)

                           tataTGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCACGATGTTGTTTCTTTACg                                                                                                            <  1:157131/139‑1 (MQ=255)

                             tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  4:116316/139‑1 (MQ=255)

                             tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  4:293335/139‑1 (MQ=255)

                              atGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGc                                                                                                         >  6:318932/1‑139 (MQ=255)

                               tGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGc                                                                                                         <  4:347047/138‑1 (MQ=255)

                                ggTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTTTTTCTTTACGTGCtt                                                                                                       >  6:237246/1‑139 (MQ=255)

                                 gTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCttt                                                                                                      >  8:285511/1‑139 (MQ=255)

                                 gTCATTTAAAATCTTTACAAATTCAACGCTATTAGGTTCGTTAGGATTTTGCCCGACATAGTCGGGGTGTTCACCGATATCTTTTATGTTCGATGAATATTTTTTATAAATACAAAGATGGTGTTTCTGTACGTGCtt                                                                                                       >  1:293658/1‑138 (MQ=255)

                                  tCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                    <  5:346039/108‑1 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                    >  4:70552/1‑105 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  5:118708/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  8:64717/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  7:230169/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  3:40681/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  3:346677/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTCCCCGACATAGTCGGGGTGTTCAACGATATCATTTATGTGCGAAGAATATTTTTTATAAATACCAGGATGTAGTATCTTTACGTGCTTaata                                                                                                   >  2:339838/1‑138 (MQ=255)

                                     tttAAAATCTTTACCAATTAAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  7:170482/1‑138 (MQ=255)

                                       tAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAAt                                                                                                >  7:54516/1‑139 (MQ=255)

                                              tttACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGa                                                                                          >  6:259227/1‑138 (MQ=255)

                                                tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACAAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        >  7:307401/1‑138 (MQ=255)

                                                       tCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTa                                                                                >  6:91851/1‑139 (MQ=255)

                                                         aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:100410/138‑1 (MQ=255)

                                                         aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:182531/138‑1 (MQ=255)

                                                         aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:61408/138‑1 (MQ=255)

                                                          aCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACAt                                                                             <  7:148792/139‑1 (MQ=255)

                                                           cGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATc                                                                            >  1:272881/1‑139 (MQ=255)

                                                             cTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     <  1:215681/80‑1 (MQ=255)

                                                                ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  8:172147/1‑136 (MQ=255)

                                                                ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                       <  3:197205/139‑1 (MQ=255)

                                                                ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGGTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAGGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                       <  8:224876/139‑1 (MQ=255)

                                                                     ttCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTg                                                                  <  3:13971/139‑1 (MQ=255)

                                                                       cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 <  7:8914/138‑1 (MQ=255)

                                                                       cTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 <  5:214695/138‑1 (MQ=255)

                                                                        tttAGGATTTTGCCGGAGTTAGTCGGGGTGTTCATCGTTATCTTTTATGTGCGATGAATATTTTTAATATATACCAGGATGTTGTTTCTTTTCGTGCTTTATAAATCCGGGATACATTTTTTCATCGTTAGAAGTgg                                                                 >  1:346074/1‑136 (MQ=255)

                                                                        tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  6:213044/1‑137 (MQ=255)

                                                                        tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  7:332630/1‑137 (MQ=255)

                                                                        tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  8:339064/1‑137 (MQ=255)

                                                                        tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  1:36962/1‑137 (MQ=255)

                                                                        tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAAGATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  2:181185/1‑137 (MQ=255)

                                                                          tAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     >  6:137594/1‑67 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  1:133676/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  6:40701/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  1:96530/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  4:17065/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  2:313401/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  4:267698/138‑1 (MQ=255)

                                                                           aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:204726/138‑1 (MQ=255)

                                                                           aGGATTTTGCACGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGAGATGAATATTTTTTTTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  3:265672/138‑1 (MQ=255)

                                                                            ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  2:125776/138‑1 (MQ=255)

                                                                              aTTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaag                                                         <  4:144190/139‑1 (MQ=255)

                                                                              aTTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaag                                                         <  5:238396/139‑1 (MQ=255)

                                                                               ttttGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  2:60584/1‑134 (MQ=255)

                                                                                  tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  2:206035/86‑1 (MQ=255)

                                                                                  tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        >  3:69403/1‑104 (MQ=255)

                                                                                  tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  1:322400/132‑1 (MQ=255)

                                                                                  tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  5:171984/1‑138 (MQ=255)

                                                                                    cccGACATAGTCGGGGGGTTCAACGATATCTTTTATGTGAGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTTCAAGTCAAGTtata                                                    <  5:373026/138‑1 (MQ=255)

                                                                                     ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATg                                                  <  5:316641/139‑1 (MQ=255)

                                                                                      cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  2:287281/139‑1 (MQ=255)

                                                                                      cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  4:193705/139‑1 (MQ=255)

                                                                                      cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAACATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  8:148369/139‑1 (MQ=255)

                                                                                      agACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGCTGTTGTGTCTTTACGTGATTTATAAATCCGGGAAACATTTTTACATAGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  4:212312/138‑1 (MQ=255)

                                                                                        aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  4:325600/139‑1 (MQ=255)

                                                                                         cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGCAGTGCAAGTCAAGTTATATGTATc                                              >  8:360529/1‑139 (MQ=255)

                                                                                             gTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCtata                                          <  6:28665/139‑1 (MQ=255)

                                                                                                 gggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGa                                      >  3:169315/1‑139 (MQ=255)

                                                                                                  ggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                       <  8:311609/105‑1 (MQ=255)

                                                                                                      ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgt                                 >  3:212834/1‑139 (MQ=255)

                                                                                                           cGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTg                                                                  >  6:62927/1‑101 (MQ=255)

                                                                                                                tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 <  8:204127/97‑1 (MQ=255)

                                                                                                                tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  6:11235/1‑139 (MQ=255)

                                                                                                                tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  7:283237/1‑139 (MQ=255)

                                                                                                                   tttATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  6:346568/110‑1 (MQ=255)

                                                                                                                     tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 <  8:116079/108‑1 (MQ=255)

                                                                                                                       tgtgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  5:149518/95‑1 (MQ=255)

                                                                                                                         tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  4:240914/1‑92 (MQ=255)

                                                                                                                         tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  3:240914/92‑1 (MQ=255)

                                                                                                                         tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg              <  5:260555/139‑1 (MQ=255)

                                                                                                                         tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg              <  2:232814/139‑1 (MQ=255)

                                                                                                                         tgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg              >  3:295391/1‑139 (MQ=255)

                                                                                                                           cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  3:115090/90‑1 (MQ=255)

                                                                                                                           cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGt                           >  7:253690/1‑124 (MQ=255)

                                                                                                                           cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGt                           <  8:253690/124‑1 (MQ=255)

                                                                                                                           cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            <  1:248987/139‑1 (MQ=255)

                                                                                                                            gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTg           >  2:263482/1‑139 (MQ=255)

                                                                                                                                      ttttATAAATACCAGGATGGTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtttattta  <  6:165096/138‑1 (MQ=255)

GCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1902‑2173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`