BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,478	G→A	intergenic (+747/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,478	0	G	A	95.0%	7277.7 / 20.7	2832	intergenic (+747/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base G (54/15); major base A (1120/1495); minor base C (136/1); total (1319/1513)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2835 total aligned reads displayed.

TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGT  >  pSIJ663_pHT315_araE_xy/5340‑5612

   ggTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGc                                                                                                                                     >  4:235469/1‑139 (MQ=255)

       aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTAt                                                                                                                                 >  8:109936/1‑139 (MQ=255)

       aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTAt                                                                                                                                 >  1:51999/1‑139 (MQ=255)

        gCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATg                                                                                                                                >  8:67548/1‑139 (MQ=255)

                          gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTGCTGGGGAGGGGGGCGGAGCCGATGGAAAAACCCCAGCAAcgc                                                                                                              >  1:128104/1‑139 (MQ=255)

                          gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                              >  4:206054/1‑139 (MQ=255)

                          gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                              >  2:12798/1‑139 (MQ=255)

                            gcgcgcACGAGGGAGCTGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                            <  3:317752/137‑1 (MQ=255)

                             agCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGGCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGc                                                                                                           <  7:26589/139‑1 (MQ=255)

                              gcgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGcc                                                                                                          >  3:359554/1‑139 (MQ=255)

                               cgcACGAGGGAGCTTCCCGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                         <  1:100266/139‑1 (MQ=255)

                               cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTTGAGCGCGGCAGGGGGGCGGAGGCTATGGAAAAACCCCAGCAACGCGGccg                                                                                                         >  5:241458/1‑138 (MQ=255)

                               cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                         >  6:146174/1‑139 (MQ=255)

                               cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                         >  3:153631/1‑139 (MQ=255)

                                 cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGGGAAGCCAATGGAAAAACTCCAGCCACGCGGCCgtt                                                                                                       >  8:292887/1‑139 (MQ=255)

                                    gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGTGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCttttt                                                                                                     >  4:328462/1‑138 (MQ=255)

                                    gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGCAGCCTATGGAAAAACGCCAGCCACGCGGCCTTTTTa                                                                                                    >  5:271190/1‑139 (MQ=255)

                                    gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCGCTGACGTGAGCGTCGATTTTTGTTTTGCTGGTCAGGGGGGGGATGCCGATTGAAAAACGCCAGCCACGCCGCCTGTTTa                                                                                                    >  6:122544/1‑139 (MQ=255)

                                    gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCATGTCGGGTTTAGCCACCTCTGACTTGCGCGTCGATTTTTTTGAAGGACGTAAGGGGGGCGAAGCCTATGGAAAAACGCCCTCAACGCCGCCatttt                                                                                                     >  4:350261/1‑138 (MQ=255)

                                     aGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                                   <  2:79676/139‑1 (MQ=255)

                                         aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGGGCAGCCGATGGAAAAACGCCAGCAACCCGGCCGTTTTACGGtt                                                                                               >  3:128094/1‑139 (MQ=255)

                                         aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGtt                                                                                               >  7:87765/1‑139 (MQ=255)

                                           cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                             <  1:298867/139‑1 (MQ=255)

                                           cTTCCAGGGGGAAACGCCGGGTAGCTTTCTCGTCCTGTCGGGTTGCGCCACCTCTGCCTGGAGCGTCGATTTTTGTGATGCGCGTCCGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTcc                                                                                             <  4:177441/139‑1 (MQ=255)

                                               cAGTGGGAAACGGATGTTATATTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCGATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggc                                                                                         <  6:205675/139‑1 (MQ=255)

                                                aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                                        <  7:34517/139‑1 (MQ=255)

                                                aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCACCAACGCGCCCTTTTTACGGTTCctggcc                                                                                        >  6:89892/1‑139 (MQ=255)

                                                     gAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttg                                                                                   <  5:301921/139‑1 (MQ=255)

                                                               gTATATTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTGGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTAGGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                                           <  1:191819/137‑1 (MQ=255)

                                                                tATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                                        <  1:213699/139‑1 (MQ=255)

                                                                tATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                                        <  5:210564/139‑1 (MQ=255)

                                                                     ttctaGGCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGGGATGCTCGTCAGGGGGGCGAAGCCTATGCAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                                   <  5:8141/136‑1 (MQ=255)

                                                                      tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                                   <  6:310143/138‑1 (MQ=255)

                                                                          gtacTGTCGGGTTTCGCAACGTCTGACTTGAGCGTCGATGTTTGTGAGTCTCGTCAGGGGGTCGAAGACTATGGAAAAACGCCAGCACCGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGt                                                               <  5:135570/135‑1 (MQ=255)

                                                                          gacaggTCGGGTTTCGCCACCTCTGACTTGAGCGTGGATTTTTGTGATGGTCGGCAGGGGGGCGAAGCCTATTGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                              <  7:1036/134‑1 (MQ=255)

                                                                          gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTTCTGGTCCGGGGGGCGCAGCCTATGGAAAAACCCCAGCAACGCCGCCTTTTTACGGTTCCTGGCCTTTTGCTGGGCTTTTGGTCACATggt                                                              >  1:326524/1‑137 (MQ=255)

                                                                          gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCTCGTCAGGGGGGCGAAGCCTATTGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCAGGCCTTTTGCTGGCCATTTTCGAACATGt                                                               >  6:173491/1‑138 (MQ=255)

                                                                          gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGTAGCCTATTGAAAAAAGCCACCAACGCGGCCTTTTTAAGGTTCCTGGCCTTTTGCTGGCCTTTTGCTAACAttt                                                               >  8:46794/1‑136 (MQ=255)

                                                                          gTCCTGTCGGGTTCCGCCACCTCTGACTTGCGCGGCGATTTTGGTGATGCGCGTCCGGGGGGCGAAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                              <  1:158837/139‑1 (MQ=255)

                                                                          gTCCTGGCGGGCTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCCGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                              <  2:329122/139‑1 (MQ=255)

                                                                            ccTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                                   <  5:331019/132‑1 (MQ=255)

                                                                             cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg                                                                                >  2:206169/1‑118 (MQ=255)

                                                                             cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgcg                                                                                                             <  3:336451/89‑1 (MQ=255)

                                                                             cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCtt                                                           >  3:83023/1‑139 (MQ=255)

                                                                             cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTACTGGCCTTTTGCTGGCCTTTTGCTCAAATgttgtt                                                           >  4:106491/1‑139 (MQ=255)

                                                                             cTGTCGGGTTTCGCCACCTCTGACGTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTAAGGAAAAACCCCCGCCACGCGGCCATTTTACGGTTCCTGGCCTTTTGCTGGCCGTTTGCTCACCTGTTCtt                                                           >  4:287141/1‑139 (MQ=255)

                                                                              tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGCAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTTCTCACATGTTCtt                                                           >  8:211437/1‑138 (MQ=255)

                                                                              tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGGCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTGCTGGCCTTTTGCTGGCCGTTTGCTCACATGTTCtt                                                           >  8:187993/1‑138 (MQ=255)

                                                                                tCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTGGTGAGGGGGGCGCAGCCTATGGAAAAACGCCAGCCACGCCGGCGTTTTACGGTTCCTGGCCTGTTGCTGGCGGTTTGGTGGCATGttggtt                                                          >  1:359625/1‑133 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATTGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc                                                        >  4:154434/1‑139 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACg                                                                                                  <  4:262098/97‑1 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc                                                        >  7:20964/1‑139 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc                                                        >  5:157663/1‑139 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                                         <  7:58132/138‑1 (MQ=255)

                                                                                tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTAGTCAGGGGGGCGAATCCTATGGAAAAACCCCCACAACGCGGCCTTTTTTAGGTTACTTGCCTTTTTCTGGGCTTTTTGTAACATGTTCtttgc                                                        >  2:289367/1‑137 (MQ=255)

                                                                                 cGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCt                                                       <  8:319013/139‑1 (MQ=255)

                                                                                     tttCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGGTCGTAAGGGGGGCGAAGCCTATTGAAAAACGCCCGCAAACCCGCCGTTTTACGGTTACATGCCGTTTTCTTGCCGTTTTCTCACATTTTATTTCCttcct                                                   >  4:308999/1‑135 (MQ=255)

                                                                                     tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGt                                                   <  3:270043/139‑1 (MQ=255)

                                                                                       tCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGtt                                                  <  3:52210/138‑1 (MQ=255)

                                                                                        cGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCCGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                                 <  3:20292/138‑1 (MQ=255)

                                                                                        cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                                 <  5:64171/138‑1 (MQ=255)

                                                                                               gctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGa                                         <  8:142742/138‑1 (MQ=255)

                                                                                                   aCTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt                                     <  6:64924/139‑1 (MQ=255)

                                                                                                   aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt                                     <  3:105922/139‑1 (MQ=255)

                                                                                                   aCTTGAGCGGCGTTTTTGGTGATGCGCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCTACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                                       <  1:242080/137‑1 (MQ=255)

                                                                                                       gAGCGTCGATTTTTGTGAAGCTCGTCAGGGGGGCGAAGCCAATGGAAAAACCCCAGCCACGCGGCCTTTTTACCGTTCCTGGCCTTTTGCTTGCCTTTTGATCACATGTTCTTTCCTCCGGTATCCCCAGATTccggt                                  >  5:110417/1‑134 (MQ=255)

                                                                                                        aGCGTCGATTTTTTTGATGGTCGTCAGGGGGGCGAAGCCTATTGAAAAACCCCACCAACCCGGCCGGTTTAAGGTTACTGGCCTTTTTGTGTGCTTTTGCTCACATGTTCTTTACTGGGTTATACCCAGATTATGTggg                                >  6:228400/1‑138 (MQ=255)

                                                                                                        aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTTGCCTTTTGCTCACATGTTCTTTACTGCGTTATACCCTGATTCTGTTGa                                >  6:337417/1‑139 (MQ=255)

                                                                                                        aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                                   <  8:235294/104‑1 (MQ=255)

                                                                                                        aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                              >  8:168266/1‑109 (MQ=255)

                                                                                                        aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTc                                                             <  7:232993/110‑1 (MQ=255)

                                                                                                        aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                                >  7:122773/1‑139 (MQ=255)

                                                                                                         tcGTCGATTTTTGTGATGCGCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  7:292887/138‑1 (MQ=255)

                                                                                                         gcggcgATTTTTGGGATGCTGGTCAGGGGGGCGAAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  2:172724/139‑1 (MQ=255)

                                                                                                         gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  4:88611/139‑1 (MQ=255)

                                                                                                         gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  3:175549/139‑1 (MQ=255)

                                                                                                         gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  5:358912/139‑1 (MQ=255)

                                                                                                          cGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               <  2:38554/138‑1 (MQ=255)

                                                                                                           gTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                               >  7:367255/1‑137 (MQ=255)

                                                                                                           gTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                             <  2:256237/139‑1 (MQ=255)

                                                                                                           gTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                             <  1:71114/139‑1 (MQ=255)

                                                                                                            gcGATTTTTGTGATGCGCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAc                            <  3:250446/138‑1 (MQ=255)

                                                                                                             cGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                                   >  7:316282/1‑99 (MQ=255)

                                                                                                             cGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCt                                                       <  4:68351/111‑1 (MQ=255)

                                                                                                             cGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc                           >  7:156791/1‑139 (MQ=255)

                                                                                                             cGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc                           >  5:178655/1‑139 (MQ=255)

                                                                                                                    tgtgATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa                     <  4:131684/138‑1 (MQ=255)

                                                                                                                     gttctGCTCGTCAGGTGGGGGATGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATACCCTGATTCTGTGGATAAGCGTAtttc                    <  6:363809/134‑3 (MQ=255)

                                                                                                                         tGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTca                                                                    >  4:38447/1‑86 (MQ=255)

                                                                                                                           cTCGTCAGGTGTGCGAAGCCTATGGAAACACTCCAGCAACGCGGCATTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGGTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt              <  2:23615/138‑1 (MQ=255)

                                                                                                                           cTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCttt             >  6:271609/1‑139 (MQ=255)

                                                                                                                              gTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgag          >  1:93750/1‑139 (MQ=255)

                                                                                                                                 aGGGGGGCGAAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtga       <  7:187993/139‑1 (MQ=255)

                                                                                                                                 aGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtga       <  8:354253/139‑1 (MQ=255)

                                                                                                                                 aGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtga       <  3:300377/139‑1 (MQ=255)

                                                                                                                                  ggggggCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtgag      <  8:265353/139‑1 (MQ=255)

                                                                                                                                     gggCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                                         <  2:54977/85‑1 (MQ=255)

                                                                                                                                     gggCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGtt                                                  <  6:101411/92‑1 (MQ=255)

                                                                                                                                     gggCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                             >  1:264625/1‑113 (MQ=255)

                                                                                                                                      ggCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGt  >  3:228250/1‑139 (MQ=255)

TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGT  >  pSIJ663_pHT315_araE_xy/5340‑5612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`