Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,045	T→C	intergenic (‑159/‑142)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,045	0	T	C	99.0%	370.2 / NA	99	intergenic (‑159/‑142)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (42/56);  minor base A (0/1);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GT‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAG  >  CP006881/3722910‑3723177                                                                                                                                        |                                                                                                                                         aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                        >  5:97374/1‑138 (MQ=255) aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                        >  1:305959/1‑138 (MQ=255) aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCAGAAATCAt                                                                                                                                        >  8:267754/1‑138 (MQ=255) aCAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTCTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAt                                                                                                                                        >  5:356389/1‑138 (MQ=255)  cAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGa                                                                                                                                      <  6:315455/139‑1 (MQ=255)    aaaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATa                                                                                                                                    <  7:187094/139‑1 (MQ=255)    aaaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATa                                                                                                                                    <  4:315222/139‑1 (MQ=255)     aaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAg                                                                                                                                   >  2:165625/1‑139 (MQ=255)     aaaaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAg                                                                                                                                   >  6:357198/1‑139 (MQ=255)                  ttCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAc                                                                                                                      <  6:149840/139‑1 (MQ=255)                                  ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTaaa                                                                                                   <  5:357198/139‑1 (MQ=255)                                  ggACACAGCGATCCTTCTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTaaa                                                                                                   <  6:356389/139‑1 (MQ=255)                                     cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGATTATTCAGATTAAGAATACGCTTTCATCATAAATCATTATAGCGTATTCGTCAACT‑AT‑TATTT‑TATTTAAATAg                                                                                                >  2:278359/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               <  6:97374/139‑1 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               <  2:360778/139‑1 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  3:327794/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  2:107999/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               <  4:327794/139‑1 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  1:360778/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  5:83431/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               <  5:61796/139‑1 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  5:364596/1‑139 (MQ=255)                                      acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCACTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  3:92503/1‑139 (MQ=255)                                        aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGttt                                                                                             >  2:123563/1‑139 (MQ=255)                                        aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGttt                                                                                             >  8:100576/1‑139 (MQ=255)                                           gATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGAt                                                                                          <  8:281056/139‑1 (MQ=255)                                            aTCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATa                                                                                         >  4:45070/1‑139 (MQ=255)                                             tgcTTTTTCATTTAAGCAAATTTTAGGTCTTGCCAGCTTTACCAGTCACAATGACGCTTATTAAGATTAAGAATACGCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATat                                                                                        <  2:145430/137‑2 (MQ=255)                                             tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATat                                                                                        <  8:355930/139‑2 (MQ=255)                                             tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATa                                                                                         >  2:69233/1‑138 (MQ=255)                                                  tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcat                                                                                   >  7:341688/1‑137 (MQ=255)                                                  tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcat                                                                                   >  4:164042/1‑137 (MQ=255)                                                     cATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcatgt                                                                                 <  4:355052/138‑5 (MQ=255)                                                         tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               >  4:80598/1‑120 (MQ=255)                                                         tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                               <  3:80598/120‑1 (MQ=255)                                                                          ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGtt                                                                                              <  2:75333/104‑1 (MQ=255)                                                                          ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGtt                                                                                              >  1:75333/1‑104 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTc                                                                                                                            <  6:34961/75‑1 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTc                                                                                                                            >  5:34961/1‑75 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATATTTTTATATAAAATGTAGACAAAAATTCGTAAAAATTaat                                                         >  6:207002/1‑137 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                         >  5:81845/1‑137 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                          <  6:132669/138‑2 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                          <  8:124217/138‑2 (MQ=255)                                                                            gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                          <  1:123563/138‑2 (MQ=255)                                                                            gCCTGCTTTACAAGTCACAATCCCGCTAATTCAGATTAAGAATACGCTTTCATCATAAATCATGATACCGTTTTCGTCAACT‑ATAT‑TAT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                         >  4:218361/1‑137 (MQ=255)                                                                            gCCTGCGTTACCAGTCACAATCCCGCTTAATCAGAATAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAAATCGTaaaaataaa                                                          <  2:342391/138‑5 (MQ=255)                                                                                 cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTTATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                     >  3:193839/1‑138 (MQ=255)                                                                                 cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                     <  5:157871/138‑1 (MQ=255)                                                                                 cTTTACCAGTCACAATCCCGCATATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                     <  2:129027/138‑1 (MQ=255)                                                                                    tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                   <  5:28321/137‑1 (MQ=255)                                                                                    tACCAGTCACAATCCCGCATATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                  <  6:28021/138‑1 (MQ=255)                                                                                      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                <  7:253747/138‑3 (MQ=255)                                                                                         gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                             >  8:304629/1‑138 (MQ=255)                                                                                          tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                           <  4:352789/139‑1 (MQ=255)                                                                                          tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                           <  5:255628/139‑1 (MQ=255)                                                                                           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                           >  1:134043/1‑138 (MQ=255)                                                                                           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑AT‑T‑TTTATAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATAGTGAAATACtttc                                           >  6:255628/1‑136 (MQ=255)                                                                                            acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                         <  8:64825/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGACTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  6:338324/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  7:100576/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  1:107999/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  1:127838/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  1:165625/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  7:310102/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  7:270484/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  6:83431/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  3:164042/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTATTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  5:264657/139‑1 (MQ=255)                                                                                               aTCCCGCTTATTCAGATTAAGAATACGCATTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTATATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  3:105568/139‑1 (MQ=255)                                                                                               aTCCCACTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  4:92503/139‑1 (MQ=255)                                                                                                tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGAGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATTTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      >  1:57595/1‑138 (MQ=255)                                                                                                  ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                    <  2:305959/138‑1 (MQ=255)                                                                                                  cagCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                    <  1:278359/136‑1 (MQ=255)                                                                                                   cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TATTTAAATATTTTGATATAACATTTAGACAAAAATACGTAAAAATTAATTGTGATATACTTCACAataaggt                                  >  3:257578/1‑135 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  2:263562/134‑1 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      >  1:263562/1‑134 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      >  1:310993/1‑134 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  2:310993/134‑1 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                 <  8:341688/139‑1 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                 <  6:364596/139‑1 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                 <  7:304629/139‑1 (MQ=255)                                                                                                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                 <  6:47472/139‑1 (MQ=255)                                                                                                     attATTCATATTAAGAATAATATTTCATCATTAATAATGATAGAGTTTTAGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAAATTTAGTCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                 <  2:134043/137‑1 (MQ=255)                                                                                                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                               <  1:69233/137‑1 (MQ=255)                                                                                                         ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                            <  4:48731/139‑1 (MQ=255)                                                                                                            aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                          <  6:81845/138‑1 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                <  7:199013/114‑3 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                <  7:194478/114‑3 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                >  8:194478/1‑112 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                >  8:199013/1‑112 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      <  2:17858/124‑1 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                      >  1:17858/1‑124 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                       >  8:161162/1‑139 (MQ=255)                                                                                                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                       >  1:87316/1‑139 (MQ=255)                                                                                                               ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGc                      >  3:70744/4‑139 (MQ=255)                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt              >  1:274451/1‑138 (MQ=255)                                                                                                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt              >  8:138125/1‑138 (MQ=255)                                                                                                                               atcatAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa       <  6:328454/138‑1 (MQ=255)                                                                                                                                   tAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTATTTAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGATATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg  >  2:97765/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                         ACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GT‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAG  >  CP006881/3722910‑3723177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑