Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,406	C→A	L74M (CTG→ATG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,406	0	C	A	100.0%	167.1 / NA	49	L74M (CTG→ATG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/36);  total (13/36)

TCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTA‑TTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTG  >  CP006881/3723272‑3723501                                                                                                                                       |                                                                                                 ttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTgatgat                                                                                              >  5:273297/6‑139 (MQ=255)   cggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTg                                                                                            >  3:83837/4‑139 (MQ=255)           tttGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACg                                                                                      >  2:29444/1‑137 (MQ=255)               gCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg                                                                                <  1:255385/139‑1 (MQ=255)               gCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg                                                                                <  7:307792/139‑1 (MQ=255)                    tatatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGat                                                                           >  1:696/5‑139 (MQ=255)                         ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGattta‑tt                                                                     >  4:93149/1‑139 (MQ=255)                                ttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGc                                                              >  2:27554/5‑139 (MQ=255)                                 tttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCGGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCa                                                             >  3:263565/4‑139 (MQ=255)                                  ttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTAGGGGCCGCTATTCTGATGATTGCATACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                            >  6:244237/3‑139 (MQ=255)                                  ttcgCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                            >  2:9041/4‑139 (MQ=255)                                   tggCACTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                            <  1:166330/137‑1 (MQ=255)                                    ggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAac                                                           <  4:73215/138‑1 (MQ=255)                                       aTTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAacacct                                                       <  7:220083/139‑3 (MQ=255)                                       aTTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAacacct                                                       <  2:197395/139‑3 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  4:150784/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  5:7500/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  6:302774/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  3:251705/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  7:161162/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  7:325329/139‑1 (MQ=255)                                            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                  <  1:141214/139‑1 (MQ=255)                                              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                <  6:12450/139‑1 (MQ=255)                                              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                <  5:244237/139‑1 (MQ=255)                                              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                <  5:287708/139‑1 (MQ=255)                                              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGAGTTATGATTACGGAGGCGGTGAATTATTTTACGGGC‑ACACCtttttttt                                                >  6:229118/1‑139 (MQ=255)                                                gcaagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCa                                              >  2:33282/1‑139 (MQ=255)                                                 caagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCAc                                             <  1:206831/139‑1 (MQ=255)                                                         aCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACcg                                     <  7:75612/139‑1 (MQ=255)                                                                    tttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaa                           <  4:320298/138‑1 (MQ=255)                                                                    tttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaa                           <  5:58819/138‑1 (MQ=255)                                                                                  aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCg            >  5:21287/1‑139 (MQ=255)                                                                                  aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCGTATGATTGCTTACGGAGGCCGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCATCTGGCg            >  8:128513/1‑139 (MQ=255)                                                                                     ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  7:253893/139‑1 (MQ=255)                                                                                     ctcCGTTTGCCTTTCCGGCAGCCGCGGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  3:207375/139‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  5:66239/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  1:27554/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  1:29444/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  1:33282/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  2:696/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  3:127941/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  3:93149/136‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca       <  6:273297/138‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACAC‑CTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca       <  3:79005/137‑1 (MQ=255)                                                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGACGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  1:9041/136‑1 (MQ=255)                                                                                        cGTTTGCATTTCCGGCAGCCGCTGTCACTTTCGGGGCAGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga         <  8:119434/136‑1 (MQ=255)                                                                                         gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAcc     <  4:83837/139‑1 (MQ=255)                                                                                            tGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta  >  7:244403/1‑138 (MQ=255)                                                                                             gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAccata  <  3:127431/135‑4 (MQ=255)                                                                                             gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta  <  4:336772/135‑2 (MQ=255)                                                                                                                                       |                                                                                                 TCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTA‑TTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTG  >  CP006881/3723272‑3723501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑