Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,801,996	G→A	A327T (GCC→ACC)	U712_14440 →	UPF0118 membrane protein ytvI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,801,996	0	G	A	100.0%	160.0 / NA	48	A327T (GCC→ACC)	U712_14440	UPF0118 membrane protein ytvI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

TATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAAAA  >  CP006881/2801862‑2802130                                                                                                                                       |                                                                                                                                       tATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTAAGCGGCCGCTGACAGAGCCCAAGATACTAAGTAAATATATCGGGATAGATCCGCTGGCAACACACAACACCCt                                                                                                                                    >  4:306551/1‑139 (MQ=255)       ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                                              <  1:193222/139‑1 (MQ=255)                       ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc                                                                                                              <  1:150775/139‑1 (MQ=255)                         aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCATAATCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGAGCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            <  1:237943/139‑1 (MQ=255)                                     ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt                                                                                                <  3:306551/139‑1 (MQ=255)                                           tCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTg                                                                                           <  5:354938/138‑1 (MQ=255)                                                tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGCGCCCAATATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                                     >  1:62024/1‑139 (MQ=255)                                                tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                                       <  3:209211/137‑1 (MQ=255)                                                tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                                     <  1:338626/139‑1 (MQ=255)                                                tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGCAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGa                                                                                      <  3:221146/138‑1 (MQ=255)                                                tACCTCGTCGTGCTTATTAAGCGGCAGCGGACAGAGCCCAAGATACAAATTAAATATATCGGGATCGATCCGCTGGCAACCCTCCTCACCCAGTTTGCCGGCGTTAAATTATTAGGGTTTTTAGGTCTGATTTCggggc                                                                                     >  2:110834/1‑137 (MQ=255)                                                   cTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCgg                                                                                  <  1:110834/139‑1 (MQ=255)                                                   cTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCgg                                                                                  <  2:185257/139‑1 (MQ=255)                                                                ttCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtcat                                                                     <  3:86835/139‑1 (MQ=255)                                                                     cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGCTCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTGTTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc                                                                 >  1:280921/1‑138 (MQ=255)                                                                     cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc                                                                 >  1:192478/1‑138 (MQ=255)                                                                     cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc                                                                <  2:51940/139‑1 (MQ=255)                                                                          gCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt                                                           <  1:187607/139‑1 (MQ=255)                                                                          gCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTGTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTCCTCGTCATCATCCCAGcg                                                           >  8:216185/1‑138 (MQ=255)                                                                                     ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCacaa                                                 >  3:102904/1‑138 (MQ=255)                                                                                        aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg                                             >  2:140752/1‑139 (MQ=255)                                                                                              tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                                        >  2:80398/1‑138 (MQ=255)                                                                                                aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa                                     <  3:265215/139‑1 (MQ=255)                                                                                                    aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa                                     >  6:122572/1‑135 (MQ=255)                                                                                                    aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa                                     <  5:122572/135‑1 (MQ=255)                                                                                                    aaTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg                                  <  2:62024/138‑1 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCAccc                                                                                                                                     >  3:342596/1‑38 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCAccc                                                                                                                                     <  4:342596/38‑1 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            >  7:40605/1‑63 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                                            <  8:40605/63‑1 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa                                 >  6:21409/1‑138 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa                                 >  7:319695/1‑138 (MQ=255)                                                                                                     aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa                                 >  2:187026/1‑138 (MQ=255)                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                                              <  6:191637/40‑1 (MQ=255)                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                                              >  5:191637/1‑40 (MQ=255)                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTCTTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata                             <  2:280921/137‑1 (MQ=255)                                                                                                          tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAATCTTTCATCACAACAGGCGCATTAACAGAAATATg                           >  8:230264/1‑139 (MQ=255)                                                                                                            gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg                          <  1:80398/138‑1 (MQ=255)                                                                                                               atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACATGCGCATTAAAAGAAATATGGTcc                       >  3:166704/1‑138 (MQ=255)                                                                                                               atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTcc                       >  6:350305/1‑138 (MQ=255)                                                                                                                    tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTa                                                                             <  8:109803/79‑1 (MQ=255)                                                                                                                    tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTa                                                                             >  7:109803/1‑79 (MQ=255)                                                                                                                      cGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCa                <  4:102904/138‑1 (MQ=255)                                                                                                                      cGCTGGCAACACTCATCACACTGATTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCa                <  6:171691/138‑1 (MQ=255)                                                                                                                                   cATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGAGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCaaaaa  >  8:88124/1‑139 (MQ=255)                                                                                                                                    aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                                       <  1:169884/53‑1 (MQ=255)                                                                                                                                    aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                                       >  2:169884/1‑53 (MQ=255)                                                                                                                                    aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCaaaaa  >  2:12162/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                       TATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAAAA  >  CP006881/2801862‑2802130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑