Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,879	T→C	I231I (ATT→ATC)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,879	0	T	C	100.0%	292.0 / NA	76	I231I (ATT→ATC)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/33);  total (43/33)

TGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTG  >  CP006881/3723746‑3724013                                                                                                                                      |                                                                                                                                        tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                                                    >  6:39393/1‑139 (MQ=255) tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                                                    >  6:353921/1‑139 (MQ=255) tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                                                    >  1:226324/1‑139 (MQ=255) tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                                                                                    >  4:282039/1‑139 (MQ=255) tgaagCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTAGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCCAGCACCCTCATACAGTGACACAGATGGGAGCAGACAGAAACTAAATCCCCGt                                                                                                                                    >  8:335291/1‑139 (MQ=255)                    cttcttTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGAc                                                                                                                  <  4:152856/138‑1 (MQ=255)                          taTTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCggg                                                                                                            <  5:134188/137‑1 (MQ=255)                          taTTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCggg                                                                                                            <  3:12888/137‑1 (MQ=255)                            tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          <  8:367834/138‑1 (MQ=255)                            tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          <  3:282039/138‑1 (MQ=255)                               ccGGCTTTGAACACAGCATTGCCTATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAtag                                                                                                       <  3:152549/138‑3 (MQ=255)                                      tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                                                 <  1:163108/136‑1 (MQ=255)                                      tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                                                 <  1:167967/136‑1 (MQ=255)                                        aaCACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATgg                                                                                              <  6:147397/138‑1 (MQ=255)                                             aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGa                                                                                        >  8:142660/1‑139 (MQ=255)                                              gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                                                       <  4:210533/139‑1 (MQ=255)                                                  tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                   >  2:17603/1‑138 (MQ=255)                                                  tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                   >  2:323710/1‑138 (MQ=255)                                                   gCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                   >  6:245085/1‑137 (MQ=255)                                                     cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                <  8:21450/139‑1 (MQ=255)                                                        tatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacaca                                                                             <  1:323710/139‑2 (MQ=255)                                                           gtgCACATTCGCCCTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                           >  5:142497/1‑138 (MQ=255)                                                            tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACtg                                                                          >  4:367440/1‑137 (MQ=255)                                                             gCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                               <  4:61759/100‑1 (MQ=255)                                                             gCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                               >  3:61759/1‑100 (MQ=255)                                                              cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             >  7:10928/1‑117 (MQ=255)                                                              cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             <  8:10928/117‑1 (MQ=255)                                                              cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                       >  1:325520/1‑139 (MQ=255)                                                              cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                       >  5:80418/1‑139 (MQ=255)                                                                   tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGa                                                                  >  3:6478/1‑139 (MQ=255)                                                                     gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                                                                                            <  2:180052/111‑1 (MQ=255)                                                                     gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                                                                                            >  1:180052/1‑111 (MQ=255)                                                                             gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          >  1:275611/2‑89 (MQ=255)                                                                             gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          <  2:275611/88‑1 (MQ=255)                                                                             gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa                                                         >  1:181162/2‑138 (MQ=255)                                                                                 ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACt                                                   >  5:280129/1‑139 (MQ=255)                                                                                     tCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCa                                               <  6:138446/139‑1 (MQ=255)                                                                                      ccaGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGTAATTGTTATGATGGGCTGGATGTACTACACACTGAATCATGATCAATAAAAAA‑ACTTCCa                                               <  7:142660/136‑1 (MQ=255)                                                                                       gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             <  1:158357/92‑1 (MQ=255)                                                                                       gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             >  4:352912/1‑92 (MQ=255)                                                                                       gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             >  2:158357/1‑92 (MQ=255)                                                                                       gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                             <  3:352912/92‑1 (MQ=255)                                                                                               tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc                                                                      >  4:102926/1‑107 (MQ=255)                                                                                               tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc                                                                      <  3:102926/107‑1 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                               <  4:123777/58‑1 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                                                                                               >  3:123777/1‑58 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          >  3:23844/1‑63 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                                                                                          <  4:23844/63‑1 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAtag                                                                                                       >  7:247179/1‑64 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAtag                                                                                                       <  8:247179/66‑3 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg                                                                                                  >  2:197045/1‑71 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg                                                                                                  <  1:197045/71‑1 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                             >  7:354718/1‑139 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                             >  7:231051/1‑139 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                             >  7:83513/1‑139 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                             >  5:289230/1‑139 (MQ=255)                                                                                                       tGACACTGATGGGAGCAGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                             >  7:283482/1‑139 (MQ=255)                                                                                                         acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGt                            >  5:22951/7‑138 (MQ=255)                                                                                                           actgaTGGGCGCAGTCACAAACTTAATCCCCTTTACGCTCGGCAATCTGACCGCGTGAAAAGTTATGATGGTCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                         <  6:289230/135‑1 (MQ=255)                                                                                                               aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAC‑ACTTCCAGAGATCACTCCCTg                                 <  6:117691/127‑1 (MQ=255)                                                                                                               aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTg                                 >  5:117691/1‑127 (MQ=255)                                                                                                               aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                         >  6:225644/1‑135 (MQ=255)                                                                                                               aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                         <  5:225644/135‑1 (MQ=255)                                                                                                                 gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                   <  2:181162/139‑1 (MQ=255)                                                                                                                 gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                   >  4:298126/1‑139 (MQ=255)                                                                                                                 gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACAGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                   >  3:366116/1‑139 (MQ=255)                                                                                                                    aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                          <  6:368786/113‑1 (MQ=255)                                                                                                                    aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                          >  5:368786/1‑113 (MQ=255)                                                                                                                    aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                >  4:321984/1‑139 (MQ=255)                                                                                                                       aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc             >  5:205679/1‑139 (MQ=255)                                                                                                                         tCAGATCCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTAGCCCCGGCATCCTGATCAATAAAAAA‑ACTTCCAGCGCTCACTCCCTGCAAGTCGAAGCTGTTATTcc             >  7:18478/1‑137 (MQ=255)                                                                                                                         tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTa                                                                                    <  4:352506/67‑1 (MQ=255)                                                                                                                         tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGaa                                                                        <  1:48303/79‑1 (MQ=255)                                                                                                                             aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga           >  4:200430/1‑135 (MQ=255)                                                                                                                                   aaTCCCCGTTACGGGCGGCCATCAGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGCATCCTGATAAATAAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCtt   <  2:226324/138‑1 (MQ=255)                                                                                                                                   aaTCCCCGTTACGCTCTGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCAGCAAGTCAAAGCGGTTATTCCGAGAGATCttc  >  3:129355/1‑137 (MQ=255)                                                                                                                                      |                                                                                                                                        TGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTG  >  CP006881/3723746‑3724013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑