| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,723,912 | G→A | M242I (ATG→ATA) | U712_19195 → | putative transporter ywcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,723,912 | 0 | G | A | 98.5% | 201.3 / NA | 66 | M242I (ATG→ATA) | U712_19195 | putative transporter ywcJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (35/30); minor base T (0/1); total (35/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGC > CP006881/3723783‑3724032 | tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga < 1:163108/136‑1 (MQ=255)tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga < 1:167967/136‑1 (MQ=255) aaCACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATgg < 6:147397/138‑1 (MQ=255) aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGa > 8:142660/1‑139 (MQ=255) gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt < 4:210533/139‑1 (MQ=255) tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac > 2:323710/1‑138 (MQ=255) tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac > 2:17603/1‑138 (MQ=255) gCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac > 6:245085/1‑137 (MQ=255) cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac < 8:21450/139‑1 (MQ=255) tatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacaca < 1:323710/139‑2 (MQ=255) gtgCACATTCGCCCTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt > 5:142497/1‑138 (MQ=255) tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACtg > 4:367440/1‑137 (MQ=255) cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg < 8:10928/117‑1 (MQ=255) cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg > 7:10928/1‑117 (MQ=255) cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt > 5:80418/1‑139 (MQ=255) cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt > 1:325520/1‑139 (MQ=255) tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGa > 3:6478/1‑139 (MQ=255) gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc < 2:180052/111‑1 (MQ=255) gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc > 1:180052/1‑111 (MQ=255) gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa > 1:181162/2‑138 (MQ=255) ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACt > 5:280129/1‑139 (MQ=255) tCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCa < 6:138446/139‑1 (MQ=255) ccaGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGTAATTGTTATGATGGGCTGGATGTACTACACACTGAATCATGATCAATAAAAAA‑ACTTCCa < 7:142660/136‑1 (MQ=255) gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg > 4:352912/1‑92 (MQ=255) gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg < 1:158357/92‑1 (MQ=255) gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg < 3:352912/92‑1 (MQ=255) gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg > 2:158357/1‑92 (MQ=255) tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc > 4:102926/1‑107 (MQ=255) tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc < 3:102926/107‑1 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg > 2:197045/1‑71 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg < 1:197045/71‑1 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg > 7:354718/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg > 7:231051/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg > 5:289230/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg > 7:83513/1‑139 (MQ=255) tGACACTGATGGGAGCAGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg > 7:283482/1‑139 (MQ=255) acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGt > 5:22951/7‑138 (MQ=255) actgaTGGGCGCAGTCACAAACTTAATCCCCTTTACGCTCGGCAATCTGACCGCGTGAAAAGTTATGATGGTCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa < 6:289230/135‑1 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAC‑ACTTCCAGAGATCACTCCCTg < 6:117691/127‑1 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTg > 5:117691/1‑127 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa < 5:225644/135‑1 (MQ=255) aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa > 6:225644/1‑135 (MQ=255) gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt > 4:298126/1‑139 (MQ=255) gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt < 2:181162/139‑1 (MQ=255) gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACAGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt > 3:366116/1‑139 (MQ=255) aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt < 6:368786/113‑1 (MQ=255) aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt > 5:368786/1‑113 (MQ=255) aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt > 4:321984/1‑139 (MQ=255) aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc > 5:205679/1‑139 (MQ=255) tCAGATCCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTAGCCCCGGCATCCTGATCAATAAAAAA‑ACTTCCAGCGCTCACTCCCTGCAAGTCGAAGCTGTTATTcc > 7:18478/1‑137 (MQ=255) tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTa < 4:352506/67‑1 (MQ=255) tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGaa < 1:48303/79‑1 (MQ=255) aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga > 4:200430/1‑135 (MQ=255) aaTCCCCGTTACGGGCGGCCATCAGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGCATCCTGATAAATAAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCtt < 2:226324/138‑1 (MQ=255) aaTCCCCGTTACGCTCTGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCAGCAAGTCAAAGCGGTTATTCCGAGAGATCttc > 3:129355/1‑137 (MQ=255) atccccGTTACGCTCGGCAATCTTACCGCGGGATTATTTATGATGGGCTGGTTGTACTACACCCTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa < 2:325520/136‑1 (MQ=255) atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa < 7:63782/136‑1 (MQ=255) atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa < 8:354718/135‑1 (MQ=255) ttACGCTCGGCAATCTCACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGAGAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg < 3:312378/139‑1 (MQ=255) cgcTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGAc < 8:283482/137‑1 (MQ=255) ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCa < 8:231051/139‑1 (MQ=255) cAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc < 1:17603/139‑1 (MQ=255) tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa > 7:280283/2‑130 (MQ=255) tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa < 8:280283/129‑1 (MQ=255) cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc < 6:289045/114‑1 (MQ=255) cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc > 5:289045/1‑114 (MQ=255) | CGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGC > CP006881/3723783‑3724032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |