| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 93.5% | 77.8 / ‑5.7 | 31 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (23/6); minor base C (2/0); total (25/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 96.8% | 87.8 / NA | 31 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/5); minor base C (0/1); total (25/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 || ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGAtt > 2:239757/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttattt > 4:189652/1‑139 (MQ=255) acactCCACACCCGAAAAGAAACACCCAACCGTTTAAAACAAAACCACCCCACCGCTTTTTTTTGGCCGGCCGC‑CTTTTT‑CGCAGCCGGTTTGCTCCGCCCCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCa < 8:264524/135‑1 (MQ=255) ccATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTATTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCa > 6:323195/1‑138 (MQ=255) acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCCAGAAAAGACGGCCGAGTTCTTTTCTGGCAAACGAGCgg > 4:236367/1‑139 (MQ=255) acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTCGCAAACTAGCgg > 5:109392/1‑139 (MQ=255) gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCCAGAAAAGACGGCCGAGTTGTTTTGGGGCCCACTAGCGGcgcg > 1:162328/1‑136 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGctc > 4:159767/1‑137 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTGTCTCGCAAACTAGCGGctc > 7:130844/1‑137 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCCTTTTCTCAGCCCCAGCCCGCAAGAAAAGACGGCCGAGGTCTTTTCGACCCAACTATCGGcgg > 1:178624/1‑135 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 1:51115/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 7:49466/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCAGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTATAGCaaaa > 8:249861/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTGTTTGGCAGGCAGC‑CGTTTT‑ATCAGCCCGTTTTCTCCGCCGCAGCCCGCAAGAAAAGACGGCCGATTGAGTTTAGACCAAACTAGCGGCACTAGCAAAAc > 5:130502/1‑139 (MQ=255) ccAACCGTTACATAAAAAAACACCCCCCCGCTTTTTTTGGCCCGGCAGCTT‑TTTT‑CGCCCCCCTTTTTCCCCCCCGCCGCCCGCAAAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 1:226258/138‑1 (MQ=255) aacgttttCATAGAACAACCACCCGCCGCTTCTTTTTGGCGGCCCGC‑CTTTTT‑AGCCGCCCGTTTGCTCAGCCCCCCCCCCCACCAAACGCGGGGCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 6:130502/134‑1 (MQ=255) ccGTTTCAAAGAAAAACACCCGGCGCCTGCTTTTGGGCAGCCCCC‑T‑TTTTAAGCAGCCGTTTTGCACCGCCGCTCCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 2:37583/138‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCATGAct > 6:324444/1‑139 (MQ=255) cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAACACTCATGActc > 4:155112/1‑139 (MQ=255) cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActc > 2:226258/1‑139 (MQ=255) atatAAAAACATCCCTCCGCTTCTTTTTGGCCGGCCGC‑CTTTTT‑ACCCGCCCTTTTTCTCCGCCGCCCCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGAt < 1:37736/139‑1 (MQ=255) aaaCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGAGTTGGTTTATCGCCAACTAGCGGCGGGAGCAAAACTCCTGACTCTAAGTTAGGCCg > 1:133170/1‑139 (MQ=255) cttTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTCGTTTCTCGCAAACTAGCGGCGCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt > 1:225884/1‑139 (MQ=255) cttTTTGGCAGGCAGC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCATGACGCGATGTTAGACCGTGGAAAGAGAATTCtt > 1:102616/1‑139 (MQ=255) ggcagC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGCCCGTGGAACGAGCATTCTTTGTGACAata > 1:143703/1‑139 (MQ=255) gcagC‑CTTTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat > 8:240950/1‑139 (MQ=255) cagC‑CTTTTT‑CGCAGCCCGTTTTCCCAGCCGCACCCCTCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATg < 3:159767/139‑1 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa > 8:291403/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTTTCACAATATGATTGAAAAAACCaa > 7:69634/1‑138 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTAACAATATGAGTGAAAAAACCaa > 1:101619/1‑138 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGCCCGTGGAAAGAGAATTCTTTGTCCCAATATGTGTGAAAAAACCAACTAGgtttttg > 1:280239/1‑137 (MQ=255) || TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |