BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,654	Δ1 bp	intergenic (+29/+96)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,654	0	T	.	99.4%	888.2 / ‑1.0	178	intergenic (+29/+96)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (75/102); minor base C (0/1); total (75/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 178 total aligned reads displayed.

TCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTC  >  BS168_reference_genome/2480509‑2480797

 cTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAt                                                                                                                                           <  1:1156994/150‑1 (MQ=255)

  tGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtt                                                                                                                                          >  2:1229022/1‑150 (MQ=255)

    aaaCATGGGCAGCAAACAAAACGTTATCTCCTAACTAGGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                        <  1:324347/150‑1 (MQ=255)

    aaaCATGGGCAGCAAACAAAACCTTATCTCCTAACTCCGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                        <  2:3783713/150‑1 (MQ=255)

    aaaCATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGCCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GGAAATGTTTAC‑AAGCAtgtt                                                                                                                                        >  2:3535232/1‑150 (MQ=255)

    aaaCATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                        <  1:2300894/150‑1 (MQ=255)

     aaCATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTc                                                                                                                                       <  2:1020994/150‑1 (MQ=255)

          gggCAGCAAACAAAACCTTATCTCCTAACTACAAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCggctt                                                                                                                                  <  1:3095711/150‑4 (MQ=255)

            gcagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGAC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Ctttt                                                                                                                               >  1:1077107/1‑150 (MQ=255)

             cagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                              <  2:3249264/150‑1 (MQ=255)

              agcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAGAAAAGCC‑GAAAATGTTTAC‑AACCATTTTCggctatttt                                                                                                                              >  2:3696323/1‑143 (MQ=255)

                  aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                                         <  2:3227045/150‑1 (MQ=255)

                  aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                                         <  1:3610458/150‑1 (MQ=255)

                   accaaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCt                                                                                                                        <  1:1282536/147‑1 (MQ=255)

                     aaaaCCTTATCTCCTAACTACGAATCAGGCACAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGa                                                                                                                      <  1:4057334/150‑1 (MQ=255)

                      aaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTTCGCTGaa                                                                                                                     >  2:3068904/1‑150 (MQ=255)

                       aaCCTTACCTCCTAACTCCGAATCAGGCTCAATGGGACCGGAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGATTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                    <  1:1101941/150‑1 (MQ=255)

                         cctaacctcCAAACTACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                                  <  2:3495812/144‑1 (MQ=255)

                         ccTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATATTCGG‑CTTTATTACGCTGAACaa                                                                                                                  >  2:4030186/1‑150 (MQ=255)

                          cTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaaa                                                                                                                 >  2:1063851/1‑150 (MQ=255)

                           ttATCTCCTAACTACGAACCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAg                                                                                                                <  2:3248653/150‑1 (MQ=255)

                              tctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                                             >  2:1177324/1‑150 (MQ=255)

                               ctcCTACCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                            <  2:557039/150‑1 (MQ=255)

                               ctcCTAACTACGAATCAGGCTCAATGGGACCGAAACAATCTTATGATCTTTTGGTGAAAGATTGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AGACATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                            >  2:2510203/1‑150 (MQ=255)

                                 ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                          <  1:893168/150‑1 (MQ=255)

                                 ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                          >  2:3981633/1‑150 (MQ=255)

                                  cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CCTTTTTACGCTGAACAAAGGTATAAc                                                                                                         >  1:1707365/1‑150 (MQ=255)

                                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGGTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACaa                                                                                                       >  2:2865010/1‑150 (MQ=255)

                                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                      <  2:157213/150‑1 (MQ=255)

                                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTGTACGCTGAACAAAGGTATAACAAt                                                                                                      >  2:3281085/1‑150 (MQ=255)

                                      cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                     >  2:273463/1‑150 (MQ=255)

                                      cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                     <  2:1034589/150‑1 (MQ=255)

                                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                                  <  1:2385488/150‑1 (MQ=255)

                                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                                  <  1:3343215/150‑1 (MQ=255)

                                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGGATAACAATCTCCTta                                                                                               >  2:2032542/1‑150 (MQ=255)

                                                ggCTCAATGGGCCCGAAAGATTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAACCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                           <  1:774220/150‑1 (MQ=255)

                                                ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                           <  1:3172014/150‑1 (MQ=255)

                                                   tCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                        >  2:2703357/1‑150 (MQ=255)

                                                    cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                                       <  1:1520076/150‑1 (MQ=255)

                                                     aaTGGGACCGAAGGAATCTGATGACCTTTTGGTGAAAGCCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                      <  2:1619425/150‑1 (MQ=255)

                                                     aaTGGGACCGAAAGAATCTGATGATGTTTCGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAACAAGCCTGAAAT‑GTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                      <  1:3555518/150‑1 (MQ=255)

                                                     aaTGGGACCGAAAGAATCTGATGACCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                      <  2:1169117/150‑1 (MQ=255)

                                                        gggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACCAAAccc                                                                                   >  2:631933/1‑150 (MQ=255)

                                                          gACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccccc                                                                                 <  1:2700233/150‑1 (MQ=255)

                                                           aCCGAAAGAACCTGATGATCTTTGGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                                <  1:960355/150‑1 (MQ=255)

                                                             cGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCaaa                                                                              <  2:2800049/150‑1 (MQ=255)

                                                              gAAGGAACCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                             <  2:684486/150‑1 (MQ=255)

                                                               aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGCAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGc                                                                            >  2:3844324/1‑150 (MQ=255)

                                                                aaGATTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                           <  1:1229022/150‑1 (MQ=255)

                                                                 aGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                          <  2:246613/150‑1 (MQ=255)

                                                                   gaTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTaa                                                                        <  1:2090908/149‑1 (MQ=255)

                                                                    aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                       <  1:1662301/150‑1 (MQ=255)

                                                                       tgatgaTCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGc                                                                    >  1:876405/1‑150 (MQ=255)

                                                                        gatgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCt                                                                   >  1:3494314/1‑150 (MQ=255)

                                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCtt                                                                  >  2:16590/1‑150 (MQ=255)

                                                                              cTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCgggg                                                             <  2:2910757/150‑1 (MQ=255)

                                                                                tttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                           <  2:3045863/150‑1 (MQ=255)

                                                                                tttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                           >  2:3188343/1‑150 (MQ=255)

                                                                                   ggTGAAAGACGGCTTACACTGGTGGAACAAATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATAGT‑GGGCTTTTTTACTCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAACAGCTTCGGGGGTTTc                                                        >  2:2219227/1‑150 (MQ=255)

                                                                                      gAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCCTCGGGGGTTTCTAt                                                     >  2:2217113/1‑150 (MQ=255)

                                                                                       aaaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAtt                                                    >  2:2757852/1‑150 (MQ=255)

                                                                                           aCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGAtt                                                <  1:306603/150‑1 (MQ=255)

                                                                                            cGGCTTACACTGGTGGAACATATAATAAGAAGAAACAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTa                                               <  1:1393181/150‑1 (MQ=255)

                                                                                             ggCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                              <  1:2547446/150‑1 (MQ=255)

                                                                                               cctagtcTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCCTTTTCTGT‑TTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                                            <  1:1494132/144‑1 (MQ=255)

                                                                                               cTTACACTGGTGGAACATATAATAAGAAGAAAAAACCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                                            <  1:1290452/150‑1 (MQ=255)

                                                                                                ttACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTc                                           >  1:3785893/1‑150 (MQ=255)

                                                                                                     cTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                      <  2:876405/150‑1 (MQ=255)

                                                                                                      tggtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGGACAAAGGTACAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGTTTCGGGGGTTTCTATTGATTACTTCATCCAt                                     >  1:520260/1‑150 (MQ=255)

                                                                                                      tggtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGATTCTATTGATTCCTTCATCCAt                                     >  1:3904084/1‑150 (MQ=255)

                                                                                                       ggtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                    >  1:1767116/1‑150 (MQ=255)

                                                                                                        gtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCTATTGATTACTTCATCCATTc                                   >  2:3609157/1‑150 (MQ=255)

                                                                                                            aaCATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTTC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                               <  1:3685042/150‑1 (MQ=255)

                                                                                                            aaCATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                               >  2:551373/1‑150 (MQ=255)

                                                                                                              cATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAACCATTCAGTATg                             >  2:2100302/1‑150 (MQ=255)

                                                                                                               ataaAATAGGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTAACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATgg                            <  1:3284206/150‑1 (MQ=255)

                                                                                                                 ataataAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGCGTTTCTATTGATTACTTCATCCATTCAGTATGGaa                          >  2:1095516/1‑150 (MQ=255)

                                                                                                                  taataaGAAGAAAAAAGGC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTCTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAg                         <  1:2960514/150‑1 (MQ=255)

                                                                                                                  taataaGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAg                         <  1:3642398/150‑1 (MQ=255)

                                                                                                                   aataaGAAGAAAGAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGa                        <  1:631933/150‑1 (MQ=255)

                                                                                                                     taaGAAGAAAAAAGCC‑GAAATTGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg                      <  2:1861348/150‑1 (MQ=255)

                                                                                                                      aagaagAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGc                     <  2:3404232/150‑1 (MQ=255)

                                                                                                                       agaagaAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGcc                    <  1:1825517/150‑1 (MQ=255)

                                                                                                                        gaagaaAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCct                   <  2:2344508/150‑1 (MQ=255)

                                                                                                                         aagaaAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCctt                  <  1:2623828/150‑1 (MQ=255)

                                                                                                                            aaaaaaGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCcttctt               >  2:3178244/1‑150 (MQ=255)

                                                                                                                              tacaGCC‑GAAAATGATTAC‑AAGCATTTTCGG‑CCAATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTg             >  2:2609983/4‑150 (MQ=255)

                                                                                                                                 aGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCa          <  1:3732422/150‑1 (MQ=255)

                                                                                                                                  gCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAg         >  2:1813211/1‑150 (MQ=255)

                                                                                                                                   cc‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGGTGCCTTCTTTGTCAGt        >  1:2741481/1‑150 (MQ=255)

                                                                                                                                   cc‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTATCAGt        >  1:2060476/1‑150 (MQ=255)

                                                                                                                                    c‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGGGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa       >  2:1530648/1‑150 (MQ=255)

                                                                                                                                    c‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa       >  2:148145/1‑150 (MQ=255)

                                                                                                                                     cggaaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa       <  2:2538521/143‑1 (MQ=255)

                                                                                                                                       aaaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGGAGATGCCTTCTTTGTCAGTACg     >  1:977244/6‑150 (MQ=255)

                                                                                                                                       aaaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACg     <  1:3004148/145‑1 (MQ=255)

                                                                                                                                        aaatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGc    >  2:3817551/5‑150 (MQ=255)

                                                                                                                                         aatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGTTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCt   <  2:520260/147‑1 (MQ=255)

                                                                                                                                         aatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCt   >  2:1187065/4‑150 (MQ=255)

                                                                                                                                         aatgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCt   >  2:1927165/4‑150 (MQ=255)

TCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTC  >  BS168_reference_genome/2480509‑2480797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`