BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	98.6%	759.6 / 2.5	216	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (107/105); total (110/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTC  >  BS168_reference_genome/165889‑166180

cccGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCCCTCCA‑TTT‑at                                                                                                                                                 >  1:982297/1‑149 (MQ=255)

  cGCTTGGAATCTTAAATGTATTATTACCAAGGTTTCTCAAAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atcc                                                                                                                                               <  1:1575602/150‑4 (MQ=255)

  cGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atcc                                                                                                                                               >  1:1830113/1‑147 (MQ=255)

  cGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atcc                                                                                                                                               >  1:3789224/1‑147 (MQ=255)

  cGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCCCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atcc                                                                                                                                               <  2:71426/150‑4 (MQ=255)

   gCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atcct                                                                                                                                              >  2:1465134/1‑146 (MQ=255)

    cTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑atccta                                                                                                                                             <  2:845891/150‑6 (MQ=255)

        gAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACgg                                                                                                                                         <  2:914893/150‑1 (MQ=255)

           gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGat                                                                                                                                      <  1:1042198/150‑1 (MQ=255)

           gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGat                                                                                                                                      >  1:2839333/1‑150 (MQ=255)

            cTTAAATGTATTATTACCAAGGTTGCTCATAAGGAGAACGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGata                                                                                                                                     <  1:1254278/150‑1 (MQ=255)

               aaaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGTATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTg                                                                                                                                  >  2:3566587/1‑150 (MQ=255)

               aaaTGTATTATTACCAAGGTTCCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTg                                                                                                                                  <  1:1674043/150‑1 (MQ=255)

                 aTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTTGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTa                                                                                                                                <  1:3034147/150‑1 (MQ=255)

                 aTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGGTATTGTa                                                                                                                                >  2:1217717/1‑150 (MQ=255)

                  tGTATTTTTACCAAGGTTTGTCAAAAGGAGAAGGCTTTTTTTATTGCGGTATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCGAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTaa                                                                                                                               <  1:3614990/150‑1 (MQ=21)

                  tGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCCCTCCATTTT‑T‑CCTAACCGGATATTGTaa                                                                                                                               >  1:3645659/1‑150 (MQ=25)

                     attattACCAAGGTTTCTGATAAGGAGAAAGCGTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAaatt                                                                                                                            <  2:2998180/150‑1 (MQ=25)

                       tattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAaattga                                                                                                                          >  1:2362256/1‑150 (MQ=255)

                           aCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                                      >  1:3156838/1‑150 (MQ=255)

                            ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTgc                                                                                                                     <  2:498761/150‑1 (MQ=255)

                              aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTgcgc                                                                                                                   >  1:3861102/1‑150 (MQ=255)

                              aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTgcgc                                                                                                                   >  2:1555262/1‑150 (MQ=255)

                                ggTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGccc                                                                                                                 >  2:3016657/1‑150 (MQ=255)

                                 gTTTCTCATAAGGAGAAAGTTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGACCACCCCCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                                                <  1:1413605/150‑1 (MQ=25)

                                  tttCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGt                                                                                                               >  1:3015320/1‑150 (MQ=255)

                                  tttCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGt                                                                                                               <  2:1965252/150‑1 (MQ=255)

                                    tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                             >  1:762050/1‑150 (MQ=255)

                                     ctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGc                                                                                                            <  2:2854087/150‑1 (MQ=255)

                                     ctcATAAGGAGAAAGCTTTTTCTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGc                                                                                                            <  1:1490367/150‑1 (MQ=255)

                                          aaGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGCAGCTCAAt                                                                                                       >  1:2797722/1‑150 (MQ=255)

                                            ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTg                                                                                                     >  2:1882043/1‑150 (MQ=255)

                                             gagaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTgg                                                                                                    <  2:65930/150‑1 (MQ=255)

                                               gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTAA‑CCTAACGGGATAGTGTAAATTGAATTGCGCCCGGAGCTCAATTGGAt                                                                                                  >  2:1171699/1‑150 (MQ=25)

                                                aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGAAATTGTAAATTGAATTGCGCCCGTAGCTCAAATGGATa                                                                                                 >  1:1768625/1‑150 (MQ=255)

                                                 atgCTTTTTTTATTGCGGTATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTACGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATag                                                                                                <  1:2471617/148‑1 (MQ=255)

                                                 aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATag                                                                                                >  2:171357/1‑150 (MQ=255)

                                                  aGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGGAATGCGGCCGTAGCTCAATTGGATaga                                                                                               >  1:194558/1‑150 (MQ=25)

                                                  aGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATaga                                                                                               >  2:3460106/1‑150 (MQ=255)

                                                   gCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                                              <  1:223618/150‑1 (MQ=255)

                                                    cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGTACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGc                                                                                             >  1:4031410/1‑150 (MQ=255)

                                                    cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAGGGTGGGGGTCGCGGGTTCGAATCCCGCCTTCCGCTCCA‑TTT‑ATCCTAACGCGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATACAGc                                                                                             <  1:2876404/150‑1 (MQ=25)

                                                     tttttttGTGGCGATATGCGGAAGAAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCg                                                                                            <  1:39509/150‑1 (MQ=25)

                                                       tttttATTGGGATCTGGCGAAGTAGGTCGGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTCCGAATCCCGTCTCCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCCCAATTGGATAGAGCggt                                                                                          <  2:1236450/150‑3 (MQ=21)

                                                         tttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCCTAGCTCCATTGGATAGGGCGTCTg                                                                                        >  1:2305877/1‑150 (MQ=21)

                                                           tATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGAc                                                                                      >  2:1775344/1‑150 (MQ=255)

                                                            aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGTGCCCGTAGCTCAATTGGGTAGAGCGTTTGACt                                                                                     >  1:3519693/1‑150 (MQ=255)

                                                                cGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACgg                                                                                 >  1:2390833/1‑150 (MQ=255)

                                                                 gATCTGCGGGAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGACTTCCGCCCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGa                                                                                <  1:3373193/150‑1 (MQ=25)

                                                                  atatGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGGAAATTGAATTGCGCCCGGAGCTCAATTGGATAGAGCGGTTGACTACgggt                                                                               >  1:1309981/1‑148 (MQ=21)

                                                                   tttGCGGAAGTAGTACAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATc                                                                              <  1:3244148/148‑1 (MQ=255)

                                                                     tggggAAGTAGTTCAGTGGAAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                            <  1:3971453/147‑1 (MQ=255)

                                                                     tGCGGAAGTAGTTCACTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGATCGAATCCCGTCCTCCGCTCCA‑TTT‑ATCCAAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                            <  1:3279496/150‑1 (MQ=25)

                                                                      gCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCCCTCCA‑TTT‑ATCCTAACGGGGTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGGTTGACTACGGGTCaaa                                                                           >  1:842478/1‑150 (MQ=25)

                                                                        ggAGGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAg                                                                         <  1:1399245/150‑1 (MQ=255)

                                                                        ggAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAg                                                                         >  1:4050128/1‑150 (MQ=255)

                                                                         gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAgg                                                                        <  2:2226000/150‑1 (MQ=255)

                                                                          aaGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGGTTGACTACGGATCAAAAGGt                                                                       >  1:1900280/1‑150 (MQ=255)

                                                                            gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                     >  1:3643829/1‑150 (MQ=255)

                                                                            gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                     >  2:3051834/1‑150 (MQ=255)

                                                                              agtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGGATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTGAgg                                                                   >  1:3347489/1‑150 (MQ=255)

                                                                                  cAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGtt                                                               <  2:1329672/150‑1 (MQ=255)

                                                                                   aGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGCCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTc                                                              <  1:2488415/150‑1 (MQ=255)

                                                                                   aGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTc                                                              >  1:1133310/1‑150 (MQ=255)

                                                                                   aGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCCCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGtgc                                                              >  1:800051/1‑148 (MQ=255)

                                                                                     tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                            >  1:2032110/1‑150 (MQ=255)

                                                                                       gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                                          <  1:3131974/150‑1 (MQ=255)

                                                                                        tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActc                                                         <  1:1882043/150‑1 (MQ=255)

                                                                                         aGACCACCACCTTGCGGAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGGGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcc                                                        <  1:34152/150‑1 (MQ=37)

                                                                                         aGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcc                                                        >  2:2164823/1‑150 (MQ=255)

                                                                                           aaCACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcctc                                                      >  1:1002130/1‑150 (MQ=255)

                                                                                                caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                                 >  1:1346950/1‑150 (MQ=255)

                                                                                                 accTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgc                                                <  2:2943756/150‑1 (MQ=255)

                                                                                                  ccctGCCAAGGTGGGGGTCGCGGGTTCGAATCGCGTCTGCCGCTCCA‑TTTGT‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTGCGGATCAAAAGGTTAGGGGTTCGACCCCTCTCGGgcg                                               <  1:2550610/147‑1 (MQ=25)

                                                                                                   cTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgc                                              >  2:867997/1‑150 (MQ=255)

                                                                                                     tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgcgc                                            <  1:52319/150‑1 (MQ=255)

                                                                                                        cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGTGCCat                                         >  1:2667869/1‑150 (MQ=255)

                                                                                                        cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCat                                         <  2:3710373/150‑1 (MQ=255)

                                                                                                           ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATc                                      <  2:2613656/150‑1 (MQ=255)

                                                                                                              gggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGTTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCttt                                   <  2:2314163/150‑1 (MQ=255)

                                                                                                               ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtttt                                  <  2:1075223/150‑1 (MQ=255)

                                                                                                               ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtttt                                  <  1:1548876/150‑1 (MQ=255)

                                                                                                                gggTCGCGGGTTCGAATCCCGCCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTa                                 <  2:1131414/150‑1 (MQ=255)

                                                                                                                 ggTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGAGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaa                                >  1:2038458/1‑150 (MQ=255)

                                                                                                                   tCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGACTTTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata                              <  1:617556/150‑1 (MQ=255)

                                                                                                                    cgcgGGTCCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatag                             <  2:2055607/150‑1 (MQ=255)

                                                                                                                     gcgGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaataga                            >  2:2361804/1‑150 (MQ=255)

                                                                                                                     gcgGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaataga                            >  2:2699935/1‑150 (MQ=255)

                                                                                                                      cgGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa                           <  2:4031410/150‑1 (MQ=255)

                                                                                                                        ggTTCGAATCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaata                         <  1:1027365/150‑1 (MQ=255)

                                                                                                                              aaTCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAgg                   <  2:3967546/150‑1 (MQ=255)

                                                                                                                               aTCCCGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGa                  >  1:144188/1‑150 (MQ=255)

                                                                                                                                 cccGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa                >  1:2374639/1‑150 (MQ=255)

                                                                                                                                 cccGTCTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa                >  1:678350/1‑150 (MQ=255)

                                                                                                                                      cTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCggg           >  1:716487/1‑150 (MQ=255)

                                                                                                                                      cTTCCGCTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCggg           <  1:1218304/150‑1 (MQ=255)

                                                                                                                                            cTCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAg     >  2:324164/1‑150 (MQ=255)

                                                                                                                                             tCCA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGc    <  1:3051834/150‑1 (MQ=255)

                                                                                                                                              ccA‑TTT‑ATCCTAACGGGATATTGAAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGAATCAAAAGTTTAGTGATTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCAGGAAGCAGCt   >  1:1534611/1‑150 (MQ=17)

                                                                                                                                               cA‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACGACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAAGAGAATAGATAGGAAATCGGGAGGTAGCTc  >  2:3802595/1‑150 (MQ=17)

CCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTC  >  BS168_reference_genome/165889‑166180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`