BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	143,659	C→T	S100F (TCT→TTT)	rpsE →	ribosomal protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	143,659	0	C	T	98.6%	1056.6 / NA	293	S100F (TCT→TTT)	rpsE	ribosomal protein S5
Reads supporting (aligned to +/- strand): ref base C (2/0); major base T (134/152); minor base G (0/4); total (136/157)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

GTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAC  >  BS168_reference_genome/143512‑143805

gTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTg                                                                                                                                                  <  2:2096562/150‑1 (MQ=255)

  aaaGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGaa                                                                                                                                                >  1:2664832/1‑150 (MQ=255)

    aGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                              <  1:3126015/150‑1 (MQ=255)

    aGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                              <  2:607397/150‑1 (MQ=255)

     gCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                                             <  2:2841700/150‑1 (MQ=255)

       acaAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTAc                                                                                                                                           >  1:603798/1‑150 (MQ=255)

         aagaagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGGACTg                                                                                                                                         >  2:992126/1‑150 (MQ=255)

           gaagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGa                                                                                                                                       >  1:53782/1‑150 (MQ=255)

             agTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGt                                                                                                                                     <  1:3228782/150‑1 (MQ=255)

                aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCGGGTAACATCTTGTTAAAGCCTGCTTTTGAAGGTACTGGAGTTAt                                                                                                                                  >  1:1484398/1‑150 (MQ=255)

                   aGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGc                                                                                                                               <  1:2720792/150‑1 (MQ=255)

                    gAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTGCCAATGGTTGGAACTCCAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACACCTAGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCt                                                                                                                              <  1:1001755/150‑1 (MQ=255)

                      aGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTgg                                                                                                                            <  2:232661/150‑1 (MQ=255)

                       gCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                           <  1:367851/150‑1 (MQ=255)

                       gCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                           >  1:363459/1‑150 (MQ=255)

                          aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGc                                                                                                                        >  2:2182317/1‑150 (MQ=255)

                          aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCAGTCGTTTCGGTGCAGGGAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGAGGTTATACCTGGAGGc                                                                                                                        >  1:844264/1‑150 (MQ=255)

                            tCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGccc                                                                                                                      <  1:174814/150‑1 (MQ=255)

                             cGCAAAGCAGGTGAAGATGCGAAAAAGAACTTGATTGAAGTACCAATGGTGGGAACTACAATTCCACACGAAATCAGCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTCTTGAAGGTACAGGAGTTAGCGCTGGAGGCCCt                                                                                                                     <  2:517532/150‑1 (MQ=255)

                              gCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTg                                                                                                                    >  2:827225/1‑150 (MQ=255)

                               cAAAGCTGTTGAGGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTga                                                                                                                   >  2:2973614/1‑149 (MQ=255)

                                aaaGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTGTCGCTGGAGGCCCTGTa                                                                                                                  >  2:888369/1‑150 (MQ=255)

                                aaaGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTa                                                                                                                  <  2:4034023/150‑1 (MQ=255)

                                   gCTGTTGAAGATGCGAAAAAGTATTTGATTGAAGTACCAATGGTTGGAACTACAATTGCACACGAGATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                               >  1:2806536/1‑150 (MQ=255)

                                     tGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGGGTTATCGCTGGAGGCCCTGTACGTGc                                                                                                             >  2:3647595/1‑150 (MQ=255)

                                       ttGAAGATGCGAAAAAGAGTTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCgg                                                                                                           <  2:961782/150‑1 (MQ=255)

                                        tGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGATGGAGGCCCTGTACGTGCGGt                                                                                                          <  1:2914017/150‑1 (MQ=255)

                                        tGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGAACTGGAGTAATCGCTGGAGGCCCTGTACGTGCGGt                                                                                                          >  2:3964840/1‑150 (MQ=255)

                                         gAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTa                                                                                                         >  1:1534225/1‑150 (MQ=255)

                                          aaGATGGGAAAAAGAATTTGATTGAAGTACCAATGGTTCGAACTACAATTCCACACGAAATCACCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTATGAAGGTACTGGAGTTATCGGTGGAGGCCCTGTACGTGCGGTAc                                                                                                        <  1:2006368/150‑1 (MQ=255)

                                              tGCGTAAAAGAATTTGATTGAAGTACCAATGGGTGGAACTACAATCCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGa                                                                                                    <  2:1068398/150‑1 (MQ=255)

                                              tGCGAAAAAGAATTTGATTGAAGTACCAAAGGTTGGAACTACAATGCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAATCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGa                                                                                                    <  1:910306/150‑1 (MQ=255)

                                                  aaaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagcta                                                                                                <  2:2786801/150‑1 (MQ=255)

                                                  aaaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagcta                                                                                                <  1:2377035/150‑1 (MQ=255)

                                                   aaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGGGCAGGTAACATCTTGTTAAAACCTGCGTTGGAAGGTAGTGGAGGTATCGCTGGGGGGCCTGTACGGGCGGGACTTGtgctag                                                                                               >  2:1307081/1‑150 (MQ=255)

                                                    aaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctagc                                                                                              <  2:2599727/150‑1 (MQ=255)

                                                      aGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTg                                                                                            >  1:2829852/1‑150 (MQ=255)

                                                      aGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTg                                                                                            <  2:937370/150‑1 (MQ=255)

                                                       gAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTgg                                                                                           >  1:1886878/1‑150 (MQ=255)

                                                       gAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTATTGAAGGTACTGGAGTTATCGCTGGAGACCCTGTACGTGCGGTACATGAGCTAGCTgg                                                                                           <  1:3580458/150‑1 (MQ=255)

                                                        agtttGATTGAAGTCCCAATGGTTGGACCTACAATTCCCCACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgt                                                                                          <  2:1798143/148‑1 (MQ=255)

                                                        aaTTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgt                                                                                          <  2:661730/150‑1 (MQ=255)

                                                           ttgattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTa                                                                                       <  2:1206002/150‑1 (MQ=255)

                                                             gattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                                     >  1:2333677/1‑150 (MQ=255)

                                                             gattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                                     <  2:701002/150‑1 (MQ=255)

                                                              attgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCt                                                                                    <  2:2694155/150‑1 (MQ=255)

                                                               ttgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTg                                                                                   >  1:1235172/1‑150 (MQ=255)

                                                                 gaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGat                                                                                 <  1:673244/150‑1 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTATAACCTGCTTTTGAAGGTACTGGAGGTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               >  1:3357489/1‑150 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               >  2:3446511/1‑150 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               <  2:1263039/150‑1 (MQ=255)

                                                                          aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGGAGCTGATATCCTTTCt                                                                        >  2:3578836/1‑150 (MQ=255)

                                                                            gggtGGAACTCCAATTCCACACGAAATCGTCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTCCTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTCGCTGGTGTAGCTGATATCCTTTCTaa                                                                      <  2:3786691/147‑1 (MQ=255)

                                                                             gTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAg                                                                     <  2:2898167/150‑1 (MQ=255)

                                                                              ttGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                                    >  1:340843/1‑150 (MQ=255)

                                                                               tGGATCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGTGCTAGCTGGTGTAGCTGATATCCTTCCTAAGTc                                                                   >  2:3915634/1‑150 (MQ=255)

                                                                                  aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCttt                                                                >  1:1378843/1‑150 (MQ=255)

                                                                                     tACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                             >  2:2508694/1‑150 (MQ=255)

                                                                                     tACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                             <  2:1946165/150‑1 (MQ=255)

                                                                                     tACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACAACTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                             <  2:3347004/150‑1 (MQ=255)

                                                                                        aaTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTACCATCTTGTTAAAACCTGCTTGTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTc                                                          <  2:1151211/150‑1 (MQ=255)

                                                                                        aaTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTc                                                          <  2:1240437/150‑1 (MQ=255)

                                                                                         aTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCt                                                         <  2:1671797/150‑1 (MQ=255)

                                                                                         aTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCt                                                         <  1:2748193/150‑1 (MQ=255)

                                                                                          ttCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGCGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTa                                                        >  2:3252521/1‑150 (MQ=255)

                                                                                          ttCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTa                                                        >  1:49367/1‑150 (MQ=255)

                                                                                                   aaaTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGa                                               <  1:874189/150‑1 (MQ=255)

                                                                                                    aaTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGAt                                              >  2:3765709/1‑150 (MQ=255)

                                                                                                     atcatcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATc                                             <  1:2177429/150‑1 (MQ=255)

                                                                                                      tcatcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCa                                            <  1:3569405/150‑1 (MQ=255)

                                                                                                       catcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCCTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCaa                                           <  2:3710716/150‑1 (MQ=255)

                                                                                                        atcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCAAAGTCTTTAGGTTCTAACACACCGATCAAc                                          <  1:993581/150‑1 (MQ=255)

                                                                                                          cGGACGTTTCGGTGCAGGTAACATCTTGTTAAACCCTGCGTGTGAAGGTACTGGGGTTACCGCTGGAGGCCCTGTACGTGGGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACAt                                        <  2:3013052/150‑1 (MQ=255)

                                                                                                           ggACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATg                                       <  1:3743280/150‑1 (MQ=255)

                                                                                                           ggACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTACCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATg                                       <  2:424896/150‑1 (MQ=255)

                                                                                                            gACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGTAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGa                                      >  1:1016381/1‑150 (MQ=255)

                                                                                                              cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGGTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAtt                                    >  1:3790711/1‑150 (MQ=255)

                                                                                                                 ttCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGGGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGGTCTAACACACCGATCAACATGATTCGt                                 >  1:115311/1‑150 (MQ=255)

                                                                                                                  tCGGTGCAGGTAACACCTTGTTAAACCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTCCGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATCCGTg                                <  2:314779/150‑1 (MQ=255)

                                                                                                                         aGGTAACATCTTGGTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTGTCTAAGTCTTTAGGTTCTAACACACCGATCAACACGATTCGTGCAACACt                         >  2:574819/1‑150 (MQ=255)

                                                                                                                           gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGGGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTc                       >  2:589153/1‑150 (MQ=255)

                                                                                                                           gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTc                       <  1:4018459/150‑1 (MQ=255)

                                                                                                                            tAACATCTTGTTAACACCTGCTTTTGAAGGTCCTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCa                      <  2:1422049/150‑1 (MQ=255)

                                                                                                                               cATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAgg                   <  2:1886878/150‑1 (MQ=255)

                                                                                                                                  cTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGttt                <  1:3449788/150‑1 (MQ=255)

                                                                                                                                  cTTGTTAAAACCTGCTGTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGttt                <  1:723448/150‑1 (MQ=255)

                                                                                                                                   ttGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTa               <  1:3301593/150‑1 (MQ=255)

                                                                                                                                      ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAgg            >  1:1303376/1‑149 (MQ=255)

                                                                                                                                      ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGt            <  2:2276916/150‑1 (MQ=255)

                                                                                                                                       tAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTg           >  1:226663/1‑150 (MQ=255)

                                                                                                                                       tAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTg           >  2:413025/1‑150 (MQ=255)

                                                                                                                                        aaaaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGa          <  1:40869/150‑1 (MQ=255)

                                                                                                                                         aaaCCTGCTTTTGAAGGTAGTGGAGTTATCGCTGGAGGCCCCGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGaa         >  1:2152350/1‑150 (MQ=255)

                                                                                                                                         aaaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGaa         <  2:1534225/150‑1 (MQ=255)

                                                                                                                                          aaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAAc        >  1:3915102/1‑150 (MQ=255)

                                                                                                                                            ccTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACtt      <  1:2508694/150‑1 (MQ=255)

                                                                                                                                            ccTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACtt      >  1:127387/1‑150 (MQ=255)

                                                                                                                                               gCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAATACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTaaa   >  1:2458232/1‑150 (MQ=255)

                                                                                                                                               gCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGAACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACCTGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTaaa   >  2:3056857/1‑150 (MQ=255)

                                                                                                                                                cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAc  >  2:3219890/1‑150 (MQ=255)

GTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAC  >  BS168_reference_genome/143512‑143805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`