Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 166,344 Δ1 bp 100% intergenic (+16/‑156) trnI‑Ala → / → rrnH‑16S tRNA‑Ala/ribosomal RNA‑16S

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome166,3440G.99.4% 590.3 / ‑0.9 310intergenic (+16/‑156)trnI‑Ala/rrnH‑16StRNA‑Ala/ribosomal RNA‑16S
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (139/169);  minor base A (1/0);  total (140/170)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 317 total aligned reads displayed.

CATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGA  >  BS168_reference_genome/166195‑166477
                                                                                                                                                     |                                                                                                                                     
caTGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAg                                                                                                                                       >  1:4738212/1‑150 (MQ=33)
 aTGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgt                                                                                                                                      <  2:1263465/150‑2 (MQ=17)
 aTGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgt                                                                                                                                      >  1:137712/1‑149 (MQ=17)
   ggTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgtta                                                                                                                                    <  2:4132727/150‑4 (MQ=17)
    gTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                                   >  1:150260/1‑146 (MQ=17)
    gTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                                   <  2:4025070/150‑5 (MQ=17)
      ttGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTg                                                                                                                                <  2:3495216/150‑1 (MQ=17)
       tGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGa                                                                                                                               <  2:4713098/150‑1 (MQ=17)
        gggACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGAc                                                                                                                              >  2:618083/1‑150 (MQ=17)
           aCCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGTGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACttt                                                                                                                           >  1:4025245/1‑150 (MQ=12)
           aCCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACttt                                                                                                                           >  2:2156192/1‑150 (MQ=17)
              aTGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTgaa                                                                                                                        <  2:4653759/150‑1 (MQ=17)
               tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTgaag                                                                                                                       >  2:577427/1‑150 (MQ=17)
                 gggTCGCAGGTTCGAATCCTGTCTTGCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTgaagaa                                                                                                                     <  1:4623614/150‑1 (MQ=12)
                    tCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                                  <  2:2377380/150‑1 (MQ=17)
                      gCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGAc                                                                                                                >  2:5004006/1‑150 (MQ=17)
                        aGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACAt                                                                                                              >  2:1744093/1‑150 (MQ=18)
                        aGGTTCGAATCCTCTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACAt                                                                                                              >  1:3422109/1‑150 (MQ=16)
                         ggTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACAtt                                                                                                             <  2:433243/150‑1 (MQ=21)
                          gTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTg                                                                                                            >  2:5710123/1‑150 (MQ=21)
                           ttCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGt                                                                                                           >  2:3970531/1‑150 (MQ=21)
                                aTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACt                                                                                                      <  2:4580740/150‑1 (MQ=21)
                                 tCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACta                                                                                                     <  2:2177036/150‑1 (MQ=21)
                                       cTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAg                                                                                               <  2:3091384/150‑1 (MQ=21)
                                       cTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAg                                                                                               >  1:1793264/1‑150 (MQ=21)
                                         tCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGtt                                                                                             <  2:25516/150‑1 (MQ=21)
                                          cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTg                                                                                            >  1:597499/1‑150 (MQ=21)
                                           ccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATGCTAATAAAGTTGc                                                                                           >  2:1471521/1‑150 (MQ=19)
                                            cGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCt                                                                                          >  2:231060/1‑150 (MQ=21)
                                             gACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCtt                                                                                         <  1:1174407/150‑1 (MQ=21)
                                             gACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCtt                                                                                         <  1:2586860/150‑1 (MQ=19)
                                               ccATTTTTTATGGGGCCTTAGCCCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTa                                                                                       <  1:5088888/150‑1 (MQ=12)
                                                cATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTTCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTaa                                                                                      <  1:3684845/150‑1 (MQ=12)
                                                  ttttttGTGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACa                                                                                    <  1:2470503/150‑1 (MQ=2)
                                                   tttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACaa                                                                                   >  1:5561621/1‑150 (MQ=11)
                                                      ttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGc                                                                                <  1:2949117/150‑1 (MQ=11)
                                                       tATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCg                                                                               <  1:4196176/150‑1 (MQ=11)
                                                       tATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCg                                                                               >  1:4828060/1‑150 (MQ=11)
                                                        aTGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCgg                                                                              <  1:4335464/150‑1 (MQ=11)
                                                         tGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGa                                                                             <  2:3805565/150‑1 (MQ=11)
                                                               cTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaaca                                                                       >  2:2992682/1‑150 (MQ=11)
                                                                  aGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACaaaa                                                                    >  1:1899267/1‑150 (MQ=11)
                                                                  aGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACaaaa                                                                    <  1:4264936/150‑1 (MQ=11)
                                                                  aGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACaaaa                                                                    <  2:4214290/150‑1 (MQ=11)
                                                                    cTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATg                                                                  <  1:1593452/150‑1 (MQ=11)
                                                                    cTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATg                                                                  >  1:5241668/1‑150 (MQ=11)
                                                                    cTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATg                                                                  <  1:2427725/150‑1 (MQ=11)
                                                                       aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTGGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAAAGATc                                                               >  2:1611097/1‑150 (MQ=2)
                                                                       aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGATCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GATATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGAACAAACAAAATGATc                                                               >  1:5745334/1‑150 (MQ=255)
                                                                         cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCCTTAACAAAGCGGACAAACAAAATGATCtt                                                             >  2:3595460/1‑150 (MQ=2)
                                                                         cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTCACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCtt                                                             >  1:4499339/1‑150 (MQ=2)
                                                                           gggAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTg                                                           <  1:268787/150‑1 (MQ=11)
                                                                             gagaGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaa                                                         <  1:469771/150‑1 (MQ=11)
                                                                                   gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaa                                                   >  1:3762040/1‑150 (MQ=11)
                                                                                   gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaa                                                   <  1:1989874/150‑1 (MQ=11)
                                                                                    ccTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaaa                                                  <  1:5625859/150‑1 (MQ=11)
                                                                                     cTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                                 <  1:97292/150‑1 (MQ=11)
                                                                                      tGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACa                                                <  2:1888110/150‑1 (MQ=11)
                                                                                       gCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACaa                                               >  2:3306626/1‑150 (MQ=11)
                                                                                        cTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAg                                              >  1:2284120/1‑150 (MQ=11)
                                                                                        cTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAg                                              >  1:3021893/1‑150 (MQ=11)
                                                                                         tttGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa                                             <  2:2870948/150‑1 (MQ=11)
                                                                                           tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa                                           <  2:5328679/150‑1 (MQ=11)
                                                                                               cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACaaaat                                       <  1:2782255/150‑2 (MQ=11)
                                                                                               cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc                                       <  2:2692286/150‑1 (MQ=11)
                                                                                               cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc                                       <  2:5603226/150‑1 (MQ=11)
                                                                                                 cAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGt                                     >  2:1099419/1‑150 (MQ=11)
                                                                                                  aggagtTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGTCTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATCATCTTTGAAAACTAAACAAGACAAAACGTa                                    <  2:5288336/150‑1 (MQ=255)
                                                                                                   ggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTAc                                   <  2:344299/150‑1 (MQ=11)
                                                                                                     aggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCt                                 >  2:2534058/1‑150 (MQ=11)
                                                                                                     aggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCt                                 <  1:5362606/150‑1 (MQ=11)
                                                                                                      ggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg                                >  2:1974776/1‑150 (MQ=11)
                                                                                                        tCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGtt                              <  1:3260976/150‑1 (MQ=11)
                                                                                                             ggTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTc                         >  1:4096866/1‑150 (MQ=11)
                                                                                                             ggTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGAGTACAAAAAAAATTATCTTTGAAAACTAATAAAGACAAAACGTACCTGTTAAttt                         >  1:310950/1‑149 (MQ=11)
                                                                                                                tCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGt                      >  1:776434/1‑150 (MQ=11)
                                                                                                                tCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGt                      <  2:2090989/150‑1 (MQ=11)
                                                                                                                 cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGtt                     <  2:5241662/150‑1 (MQ=11)
                                                                                                                  gATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttt                    >  1:1312535/1‑150 (MQ=11)
                                                                                                                  gATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttt                    >  2:5074487/1‑150 (MQ=11)
                                                                                                                  gATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttt                    >  2:812254/1‑150 (MQ=11)
                                                                                                                   aTCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGtttt                   <  2:5214954/150‑1 (MQ=11)
                                                                                                                    tCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttttt                  <  2:879298/150‑1 (MQ=11)
                                                                                                                    tCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttttt                  <  1:5472318/150‑1 (MQ=11)
                                                                                                                      ccGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaa                <  1:1016385/150‑1 (MQ=11)
                                                                                                                      ccGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaa                >  2:4876992/1‑150 (MQ=11)
                                                                                                                       cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaa               <  2:653822/150‑1 (MQ=11)
                                                                                                                       cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaa               <  1:3867001/150‑1 (MQ=11)
                                                                                                                       cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaa               >  1:359726/1‑150 (MQ=11)
                                                                                                                        gCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaa              <  2:936072/150‑1 (MQ=11)
                                                                                                                         cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTaaaaa             <  1:5446312/150‑1 (MQ=11)
                                                                                                                          tAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAAt            >  2:2055311/1‑150 (MQ=11)
                                                                                                                          tAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAAt            <  1:3296590/150‑1 (MQ=11)
                                                                                                                             gCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGc         <  1:619080/150‑1 (MQ=11)
                                                                                                                               tCCACTAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGcac       <  2:565161/150‑1 (MQ=2)
                                                                                                                               tCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGcac       >  2:5135266/1‑150 (MQ=11)
                                                                                                                                ccaccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGcaca      >  2:4756263/1‑150 (MQ=11)
                                                                                                                                 caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAg     >  2:1021411/1‑150 (MQ=11)
                                                                                                                                   ccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCg   <  2:5456373/150‑1 (MQ=11)
                                                                                                                                   ccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCg   >  2:2344541/1‑150 (MQ=11)
                                                                                                                                                     |                                                                                                                                     
CATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGA  >  BS168_reference_genome/166195‑166477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑