BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	557,865:1	+T	100%	intergenic (+416/‑8)	yddT → / → ydzN	putative exported phage protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	557,865	1	.	T	95.8%	948.4 / 25.1	263	intergenic (+416/‑8)	yddT/ydzN	putative exported phage protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (9/2); new base T (141/111); total (150/113)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 306 total aligned reads displayed.

AACGTAACAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑T‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAATATTG  >  BS168_reference_genome/557716‑558008

aaCGTAACAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑g                                                                                                                                                     <  1:3153417/150‑1 (MQ=255)

  cGTAACAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t                                                                                                                                                 <  1:233675/150‑1 (MQ=255)

     agcAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGAA‑G‑t‑t‑ttt                                                                                                                                             >  2:3558012/3‑150 (MQ=255)

     aaCAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑ttt                                                                                                                                             <  2:4524610/150‑1 (MQ=255)

      aCAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑tttt                                                                                                                                            <  1:4811691/150‑2 (MQ=255)

      aCAAGTGTGAAAGTAAAGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑tttt                                                                                                                                            >  1:3007651/1‑149 (MQ=255)

       cAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑tttta                                                                                                                                           <  2:3791914/150‑3 (MQ=255)

        aaGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑ttttaa                                                                                                                                          <  1:4915087/150‑4 (MQ=255)

        aaGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑ttttaa                                                                                                                                          >  2:5592189/1‑147 (MQ=255)

         aGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑ttttaat                                                                                                                                         <  1:5506314/150‑5 (MQ=255)

          gtgtGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t‑ttttaatt                                                                                                                                        <  2:1899297/150‑6 (MQ=255)

               aaaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGtt                                                                                                                                   <  1:2694660/150‑1 (MQ=255)

               aaaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGtt                                                                                                                                   <  1:3210117/150‑1 (MQ=255)

                  gTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGa                                                                                                                                <  2:867782/150‑1 (MQ=255)

                    aaTGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGt                                                                                                                              >  2:1088704/1‑150 (MQ=255)

                    aaTGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGAA‑GTT‑T‑TTT‑AATTGCGTTGAAGt                                                                                                                              >  2:5776726/1‑150 (MQ=255)

                           tGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGt                                                                                                                       >  2:505763/1‑150 (MQ=255)

                           tGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGt                                                                                                                       >  2:547938/1‑150 (MQ=255)

                              aGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCa                                                                                                                    >  2:294425/1‑150 (MQ=255)

                                  tCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAATTTTTATATATTTTCAATAAAATACAGGTACTATATTAAATGTATACAGATTGTATGGAGATGTAATTAATTAATGATGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAAAGt                                                                                                                <  2:3022566/150‑1 (MQ=255)

                                  ataGTTAAGAAAGGTTTAAGATTAGATATTGATAAATTTTATATATTTTCAATAAAATACTGGTATTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGt                                                                                                                <  1:977323/148‑1 (MQ=255)

                                     gTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTACTTAAACAGGA‑G‑T‑TGTTT‑AATTGCGTTGGAGTAAATGGGACAATGtttt                                                                                                             >  2:2545221/1‑150 (MQ=255)

                                     gTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTACAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                             >  2:4444303/1‑150 (MQ=255)

                                     gTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                             <  1:2094765/150‑1 (MQ=255)

                                     gTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                             >  1:1376147/1‑150 (MQ=255)

                                      ttAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGttttt                                                                                                            <  1:5012031/150‑1 (MQ=255)

                                         aGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGt                                                                                                         <  1:5266468/150‑1 (MQ=255)

                                          gAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGta                                                                                                        <  1:2550166/150‑1 (MQ=255)

                                           aaaGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGtat                                                                                                       >  2:5353581/1‑150 (MQ=255)

                                            aaGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGtata                                                                                                      <  1:3879967/150‑1 (MQ=255)

                                              ggTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTAtagt                                                                                                    <  1:5696133/150‑1 (MQ=255)

                                                  tAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCa                                                                                                >  2:3620375/1‑150 (MQ=255)

                                                  tAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCa                                                                                                <  1:1226315/150‑1 (MQ=255)

                                                  tAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCa                                                                                                >  2:1477003/1‑150 (MQ=255)

                                                   aaGATTAGATATTGAAAACTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCAt                                                                                               <  2:176838/150‑1 (MQ=255)

                                                   aaGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCAt                                                                                               <  2:3747240/150‑1 (MQ=255)

                                                       ttAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGAATGGTGATGTAATTAATTAAAAAGGT‑G‑T‑T‑TTTGAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTggg                                                                                           >  2:3662270/1‑150 (MQ=255)

                                                        tAGTTATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGa                                                                                          <  1:3478207/150‑1 (MQ=255)

                                                        tAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGGATTGGGa                                                                                          >  2:3422981/1‑150 (MQ=255)

                                                         aGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                                         >  1:1154857/1‑150 (MQ=255)

                                                           atatTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCa                                                                                       >  2:1828545/1‑150 (MQ=255)

                                                             atTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAat                                                                                     >  2:920922/1‑150 (MQ=255)

                                                                gAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                                  <  2:3879520/150‑1 (MQ=255)

                                                                 aaaaaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatatat                                                                                 <  1:4720754/150‑1 (MQ=255)

                                                                   aaaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATgg                                                                               >  2:936065/1‑150 (MQ=255)

                                                                   aaaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATgg                                                                               >  1:1288560/1‑150 (MQ=255)

                                                                    aaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGt                                                                              >  2:5266520/1‑150 (MQ=255)

                                                                     aTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGtt                                                                             <  1:1989619/150‑1 (MQ=255)

                                                                      ttttATATATTTTCAATAAAATACTGTTACTATAATAAATGTATACAGATTGTATGAAGATGTAATTAATTAAAAAGAA‑G‑TAT‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGGATTTGGATCAATATATGGTta                                                                            >  1:2412613/1‑150 (MQ=255)

                                                                       tttATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAAGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAAGATATGATtat                                                                           >  2:2238876/1‑150 (MQ=255)

                                                                           tatatTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAat                                                                       >  1:328961/1‑150 (MQ=255)

                                                                             tatTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAatta                                                                     >  2:4632953/1‑150 (MQ=255)

                                                                                tttCAATAAAATACTGGTACTATAATAAATGTATACACATTCTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTAttt                                                                  <  1:1880653/150‑1 (MQ=255)

                                                                                  tCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTAc                                                                >  2:5576744/1‑150 (MQ=255)

                                                                                   cAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACt                                                               <  1:3510501/150‑1 (MQ=255)

                                                                                    aaTAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTa                                                              >  2:2887956/1‑150 (MQ=255)

                                                                                      tAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAAc                                                            >  1:3724669/1‑150 (MQ=255)

                                                                                      tAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAAc                                                            <  1:4390038/150‑1 (MQ=255)

                                                                                              tGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGTGATCAATATATGGTTATAAAATATTTACTAACCAAGAAGt                                                    >  2:4339057/1‑150 (MQ=255)

                                                                                                gTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCa                                                  >  2:74149/1‑150 (MQ=255)

                                                                                                  aCTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGt                                                <  1:821891/150‑1 (MQ=255)

                                                                                                   cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGACT‑T‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGta                                               >  2:5104006/1‑150 (MQ=255)

                                                                                                   cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGta                                               >  1:3600217/1‑150 (MQ=255)

                                                                                                    tataATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtac                                              >  2:2986782/1‑150 (MQ=255)

                                                                                                    tataATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtac                                              >  2:124868/1‑150 (MQ=255)

                                                                                                      taataaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTAAAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAACACAGtacta                                            >  2:412637/1‑150 (MQ=255)

                                                                                                       aataaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtactac                                           >  2:2038911/1‑150 (MQ=255)

                                                                                                         taaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTc                                         <  1:2779351/150‑1 (MQ=255)

                                                                                                          aaaTGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGATTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAAGCAAGAAGTCAGTACTACTCg                                        <  1:2208139/150‑1 (MQ=255)

                                                                                                          aaaTGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCg                                        <  1:750424/150‑1 (MQ=255)

                                                                                                            aTGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGtt                                      <  1:687312/150‑1 (MQ=255)

                                                                                                                ataCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAATCAGTACTACTCGTTtaat                                  >  2:4812607/1‑150 (MQ=255)

                                                                                                                  aCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTtaataa                                >  2:683228/1‑150 (MQ=255)

                                                                                                                  aCAGATNGTATGCAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTtaataa                                <  1:2599032/150‑1 (MQ=255)

                                                                                                                   cAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTaataat                               <  1:5592189/150‑1 (MQ=255)

                                                                                                                   cAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTaataat                               <  1:628480/150‑1 (MQ=255)

                                                                                                                   cAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTaataat                               >  2:5724233/1‑150 (MQ=255)

                                                                                                                      aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGc                            >  2:5388913/1‑150 (MQ=255)

                                                                                                                       ttGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCt                           <  2:4166937/150‑1 (MQ=255)

                                                                                                                        tGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCGGTACTACTCGTTTAATAATAGCtt                          >  2:131590/1‑150 (MQ=255)

                                                                                                                        tGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCtt                          >  2:4425560/1‑150 (MQ=255)

                                                                                                                           aTGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTg                       <  1:1008258/150‑1 (MQ=255)

                                                                                                                            tGGAGATGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGt                      <  1:1802653/150‑1 (MQ=255)

                                                                                                                              gagaTGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑CATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGCAGTCAGTACTACTCGTTTAATAATAGCTTCTGTaa                    <  1:5369412/150‑1 (MQ=255)

                                                                                                                              gagaTGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTaa                    >  2:2643766/1‑150 (MQ=255)

                                                                                                                                 aTGTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttg                 <  1:4635559/150‑1 (MQ=255)

                                                                                                                                   gTAATTAATTAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGTTCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAAGAATAGCTTCTGTAAttgtg               >  2:2814053/1‑150 (MQ=255)

                                                                                                                                     aattaattAAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgtgtt             >  2:4685446/1‑150 (MQ=255)

                                                                                                                                      attaattaAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgtgttg            <  2:2479478/150‑1 (MQ=255)

                                                                                                                                      attaattaAAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTACCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgtgttg            >  1:5454311/1‑150 (MQ=255)

                                                                                                                                        taattaaAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgtgttgtg          <  2:1589573/150‑1 (MQ=255)

                                                                                                                                          attaaAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGa        >  2:4921193/1‑150 (MQ=255)

                                                                                                                                           ttaaAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTGAATGTTTTTTGTATAGTAGCATTGGGGTCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGaa       >  1:2837992/1‑150 (MQ=255)

                                                                                                                                           ttaaAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGaa       <  1:932239/150‑1 (MQ=255)

                                                                                                                                            taaAAAGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAat      <  1:4445043/150‑1 (MQ=255)

                                                                                                                                             aaaaaGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAata     >  2:5331045/1‑150 (MQ=255)

                                                                                                                                             aaaaaGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAata     >  1:1626451/1‑150 (MQ=255)

                                                                                                                                             aaaaaCGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGCATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCCTTTAATAATAGCTTCTGTAATTGTGTTGTGGaaga     >  2:2317162/1‑148 (MQ=255)

                                                                                                                                              aaaaGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAatat    >  2:3237434/1‑150 (MQ=255)

                                                                                                                                                aaGGA‑GTT‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCCTTTAATAATAGCTTCTGTAATTGTGTTGTGGAATAttg  >  2:490008/1‑150 (MQ=255)

AACGTAACAAGTGTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑T‑T‑TTT‑AATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTTGTGGAATATTG  >  BS168_reference_genome/557716‑558008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`