| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 506,071 | C→A | 20.9% | P307Q (CCG→CAG) | ydbM → | putative acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 506,071 | 0 | C | A | 20.9% | 1038.4 / 251.5 | 367 | P307Q (CCG→CAG) | ydbM | putative acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (160/130); new base A (42/35); total (202/165) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-02 | |||||||||||
Only 100 of 373 total aligned reads displayed.
AGTGCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCTATTACCGTGATG > BS168_reference_genome/505922‑506220 | gTGCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCg < 2:2894451/150‑1 (MQ=255) tGCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAgg > 2:343693/1‑150 (MQ=255) gCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGt < 2:2844997/150‑1 (MQ=255) gCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGt < 1:1990107/150‑1 (MQ=255) cAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCTCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTg < 2:4041616/150‑1 (MQ=255) cAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTg > 2:5197052/1‑150 (MQ=255) aTTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTgcg < 1:1299164/150‑1 (MQ=255) ttGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTgcggc > 1:1050533/1‑150 (MQ=255) tGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCTGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTgcggcg < 2:2473660/150‑1 (MQ=255) tGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTgcggcg < 2:1027515/150‑1 (MQ=255) tGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTgcggcg < 2:4458686/150‑1 (MQ=255) cATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGGGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTc > 1:351185/1‑150 (MQ=255) cATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTc > 2:5448059/1‑150 (MQ=255) cATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTc > 2:4367238/1‑150 (MQ=255) aTCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTcc > 1:4509272/1‑150 (MQ=255) aTCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTcc > 1:245017/1‑150 (MQ=255) cTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCcac > 2:4304692/1‑150 (MQ=255) tGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCcaca > 2:4599533/1‑150 (MQ=255) gTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACtt > 2:484915/1‑150 (MQ=255) ttCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGAc < 2:157856/150‑1 (MQ=255) ctcCAAACAGCTTGAATGACCCGGTCAGGAATGTCCCGGCCGTACAGCAAGGAACAGGCGAGATGGAACTTGAACAGCTCCATGCCAGAGACTGTCTTCACCATATCGCTCAATTGTATGACGATCCGGTGCGGGGGCCACACTTGGCCa > 2:114138/1‑150 (MQ=255) cAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGATCTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCg > 1:4313913/1‑150 (MQ=255) cAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCg > 2:274683/1‑150 (MQ=255) cAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCg > 2:2662249/1‑150 (MQ=255) cAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCg > 1:1574637/1‑150 (MQ=255) aaaCAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGCAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCACCGa > 2:5839975/1‑150 (MQ=255) gCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCg < 1:4656868/150‑1 (MQ=255) gCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCg < 2:4431139/150‑1 (MQ=255) gCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCg < 1:1643342/150‑1 (MQ=255) gCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCg > 2:5332941/1‑150 (MQ=255) gAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTgc < 1:2030699/150‑1 (MQ=255) tGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTgcggc > 2:4697167/1‑150 (MQ=255) tGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTgcggc > 2:3522647/1‑150 (MQ=255) ggACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTgcggcg > 1:2694646/1‑150 (MQ=255) ccGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGaaa < 2:4761322/150‑1 (MQ=255) gATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACa > 2:3909855/1‑150 (MQ=255) cAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGGGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACatat > 1:5581378/1‑150 (MQ=255) cAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACatat < 2:1750744/150‑1 (MQ=255) aaGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATc > 1:5669744/1‑150 (MQ=255) aaGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATc < 2:3119271/150‑1 (MQ=255) aGAATGTCCCGGCCTTACAGCAAAGGACAGGCGAAATGGAGGTTGAACTGCTCAGTGCCAGACACTTTCTTTTGCATATCGCTCAATTGTATGACGATCCGGTGCGGTGTCCAGACTTGACCAGCGAGCTCGGTGGGGCGAGACATATCg > 1:5095372/1‑150 (MQ=255) aGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCTCACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCg < 1:2782544/150‑1 (MQ=255) gAATCTCCTGGACGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTGTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACTCTTGACCAGTGAGCACGGTGCGGCGAAACATATCGt < 1:1004474/150‑1 (MQ=255) tGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTcac < 1:3909855/150‑1 (MQ=255) ggCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgc > 1:5039568/1‑150 (MQ=255) gCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgct > 2:563232/1‑150 (MQ=255) ccGAACAGCAAAGAACAGGCGAAATGGAGCTAGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCCCAATTGTATGACTATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgctg < 2:3645795/150‑1 (MQ=255) cGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgctgc > 2:5119106/1‑150 (MQ=255) gTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgctgcg < 2:369408/150‑1 (MQ=255) cAGCAAAGAACAGTCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAAcgctgcgctg > 1:5173923/1‑150 (MQ=255) aGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGctgt > 2:2704409/1‑150 (MQ=255) aaaGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGctgtctg < 2:3361666/150‑1 (MQ=255) aaGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGctgtctgt > 1:2732343/1‑150 (MQ=255) aGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCtgtt < 2:1291323/150‑1 (MQ=255) aGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCtgtt < 1:5441498/150‑1 (MQ=255) gAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCtgttg < 2:235460/150‑1 (MQ=255) gAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCtgttg < 1:1951357/150‑1 (MQ=255) aCAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTgttgtt < 2:506695/150‑1 (MQ=255) aGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGa > 2:5682729/1‑150 (MQ=255) aaaTGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAg > 1:686894/1‑150 (MQ=255) aaTGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGc < 2:83662/150‑1 (MQ=255) aTGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCg < 1:263293/150‑1 (MQ=255) cTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTc > 2:146238/1‑150 (MQ=255) cTGCTCAATGCCAGACACTTTCTTTTCCATATCGCACAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCACGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTc < 2:185516/150‑1 (MQ=255) tGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCg > 1:3667878/1‑150 (MQ=255) tGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCg < 2:3750798/150‑1 (MQ=255) cTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGGGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCggg > 1:297228/1‑150 (MQ=255) cAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGc > 2:4837531/1‑150 (MQ=255) aaTGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGACCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGcc < 2:1186694/150‑1 (MQ=255) aaTGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGcc > 1:1483026/1‑150 (MQ=255) aTGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCa < 2:2656564/150‑1 (MQ=255) tGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCaa > 1:3763859/1‑150 (MQ=255) gCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCTACACTTGACCAGCGACCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGGTGTTGATAAAGCGATGAGAATGGTCGGGGCCaaa > 1:1212287/1‑150 (MQ=255) ccAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTG‑‑‑AGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGcc > 2:5481303/1‑150 (MQ=255) cAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAg > 2:3047919/1‑150 (MQ=255) aGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGc > 1:1748811/1‑150 (MQ=255) acacTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCt < 1:936326/150‑1 (MQ=255) acTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTgg > 1:1106439/1‑150 (MQ=255) acTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAACCATGAGAATTGTCGGGGCCAAAAGACTgg > 1:2943285/1‑150 (MQ=255) ctttctttTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGga > 2:2268213/1‑150 (MQ=255) ctttctttTCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGACCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGga < 1:4617926/150‑1 (MQ=255) tttctttTCCATATCGCTCAATTGTATGACGAACCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGgaa < 2:754960/150‑1 (MQ=255) ctttTCCATATCGCTCAATTGTATTACGATCCCGTCCGGCGTCCACACTTGACCAGCGAGCCCGGTGCGGCGAAACATATCGTCACAGACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGgaacga < 2:1413799/150‑1 (MQ=255) tttCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGgaacgaac > 1:3319680/1‑150 (MQ=255) ttCCATATCGCTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGaacc < 1:2244315/150‑1 (MQ=255) tCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGaacca < 1:4924389/150‑1 (MQ=255) cATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGaaccaac < 1:3954239/150‑1 (MQ=255) cATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGaaccaac > 2:2195289/1‑150 (MQ=255) cATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGaaccaac < 1:4599533/150‑1 (MQ=255) tatCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGGTGGTGTTAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGGACCAAccc > 2:620164/1‑150 (MQ=255) atCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCg < 1:2848836/150‑1 (MQ=255) cGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCt < 2:3819416/150‑1 (MQ=255) cTCAATTGTATGACGATCAGGTGCGGCGTCCACACTTGACCAGCAAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTc < 1:4843952/150‑1 (MQ=255) cAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAg < 1:41701/150‑1 (MQ=255) cAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGACACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAg < 2:1933881/150‑1 (MQ=255) aTTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGGTGAGAAATGGCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAgcg > 1:1175355/1‑150 (MQ=255) tGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCt < 1:5260734/150‑1 (MQ=255) aTGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCTAtt < 1:3183751/150‑1 (MQ=255) aTGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCTAtt < 2:1711127/150‑1 (MQ=255) gATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCTATTACCGt > 1:2527341/1‑150 (MQ=255) | AGTGCAATTTGCATCTGAGTATTCTCCAAACAGCTTGAATGGACCGATCAAGAATGTCCCGGCCGTACAGCAAAGAACAGGCGAAATGGAGCTTGAACTGCTCAATGCCAGACACTTTCTTTTCCATATCGCTCAATTGTATGACGATCCGGTGCGGCGTCCACACTTGACCAGCGAGCTCGGTGCGGCGAAACATATCGTCACAAACGCTGCGCTGTCTGTTGTTGATAAAGCGATGAGAATTGTCGGGGCCAAAAGCCTGGAACGAACCAACCCGCTTCAGCGCTATTACCGTGATG > BS168_reference_genome/505922‑506220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |