BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,080,047	C→T	5.9%	A209V (GCC→GTC)	ecsC →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,080,047	0	C	T	5.9%	1186.0 / 30.0	340	A209V (GCC→GTC)	ecsC	putative hydrolase
Reads supporting (aligned to +/- strand): ref base C (148/169); new base T (5/16); total (155/185)
Fisher's exact test for biased strand distribution p-value = 4.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 346 total aligned reads displayed.

TAAGTCGCTTTTTTCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCTTGGCCG  >  BS168_reference_genome/1079898‑1080196

  aGTCGCTTTTTTCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGTCTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCa                                                                                                                                                     >  2:2235730/1‑150 (MQ=255)

   gTCGCTTTTTTCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCaa                                                                                                                                                    >  1:4554711/1‑150 (MQ=255)

   gTCGCTTTTTTCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCaa                                                                                                                                                    <  2:2891244/150‑1 (MQ=255)

        ttttttCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATtata                                                                                                                                               <  1:1684367/150‑1 (MQ=255)

            ttCTGTCATGGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAgct                                                                                                                                           <  1:1304008/150‑1 (MQ=255)

             tCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAgctg                                                                                                                                          >  2:3548662/1‑150 (MQ=255)

               tGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAgctgct                                                                                                                                        <  2:5409185/150‑1 (MQ=255)

                 tCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCtgcttg                                                                                                                                      >  1:3353594/1‑150 (MQ=255)

                 tCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCtgcttg                                                                                                                                      >  1:755776/1‑150 (MQ=255)

                  cATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCtgcttgc                                                                                                                                     >  2:5694946/1‑150 (MQ=255)

                   aTCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCtgcttgct                                                                                                                                    >  2:4475508/1‑150 (MQ=255)

                   aTCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGGCGGCGGAATTGCCAATTATAAGCtgcttgct                                                                                                                                    >  1:991674/1‑150 (MQ=255)

                    tCGAAAATTGGGAGACGGAGAAAAAGAACATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGTCGTCGTGAAGCGTTCTCAGCTTTTGCGCGGCCTAGGCCCGGAAGTCGGCGGAACTGCCAAATATGAGAtgattgcgc                                                                                                                                   >  2:32644/1‑148 (MQ=255)

                      gAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGGGGAATTGCCAATTATAAGCTGCTTGCTCAg                                                                                                                                 >  2:5756791/1‑150 (MQ=255)

                      gAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAg                                                                                                                                 >  2:5575773/1‑150 (MQ=255)

                        aaaTTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCt                                                                                                                               >  1:3481859/1‑150 (MQ=255)

                          aTTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTg                                                                                                                             <  1:3681366/150‑1 (MQ=255)

                            tGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGga                                                                                                                           >  2:3820947/1‑150 (MQ=255)

                            tGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGga                                                                                                                           >  1:5127834/1‑150 (MQ=255)

                             gggAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGgag                                                                                                                          <  1:1965839/150‑1 (MQ=255)

                             gggAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGgag                                                                                                                          >  1:5021963/1‑150 (MQ=255)

                                agaCGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAGCTTGGAGaaa                                                                                                                       <  2:1457682/150‑1 (MQ=255)

                                agaCGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGaaa                                                                                                                       >  1:1861684/1‑150 (MQ=255)

                                agaCGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGaaa                                                                                                                       <  2:3430573/150‑1 (MQ=255)

                                  aCGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTCTATCGACGTCGTGAAGCTTTTGCAGCTTTGCACGGGCGTAGAAGCGGCATACGGCGGCATTGCCAATTATAAGCCGCTTGCTCAGCTTGGAGTAACg                                                                                                                     <  2:91536/150‑1 (MQ=255)

                                  aCGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACg                                                                                                                     <  1:1228545/150‑1 (MQ=255)

                                   cGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACgg                                                                                                                    <  1:3737200/150‑1 (MQ=255)

                                    ggAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGc                                                                                                                   >  2:506943/1‑150 (MQ=255)

                                      agaAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGTCGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCaa                                                                                                                 >  2:4673837/1‑150 (MQ=255)

                                      agaAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCaa                                                                                                                 >  1:5360118/1‑150 (MQ=255)

                                         aaaaGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGAc                                                                                                              <  1:4902040/150‑1 (MQ=255)

                                          aaaGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGGTCAGCTTGGAGAAACGGCAAGACa                                                                                                             <  1:4632309/150‑1 (MQ=255)

                                                 aTCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGGGGAATTGCCAAGTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGttttt                                                                                                      >  2:1390418/1‑150 (MQ=255)

                                                    gACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCAt                                                                                                   >  1:2643919/1‑150 (MQ=255)

                                                    gACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCAt                                                                                                   <  2:1831896/150‑1 (MQ=255)

                                                     aCTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATc                                                                                                  >  1:5403573/1‑150 (MQ=255)

                                                     aCTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATc                                                                                                  >  1:4222800/1‑150 (MQ=255)

                                                     aCTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATc                                                                                                  >  2:4040307/1‑150 (MQ=255)

                                                      cTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCt                                                                                                 <  2:4771509/150‑1 (MQ=255)

                                                      cTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCt                                                                                                 <  1:1500417/150‑1 (MQ=255)

                                                         gagaGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAg                                                                                              <  2:2378528/150‑1 (MQ=255)

                                                           gagTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGa                                                                                            >  2:3134745/1‑150 (MQ=255)

                                                            agTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGat                                                                                           <  2:4094478/150‑1 (MQ=255)

                                                            agTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGat                                                                                           <  1:3248002/150‑1 (MQ=255)

                                                            agTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGat                                                                                           <  1:773839/150‑1 (MQ=255)

                                                             gtgtTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGata                                                                                          >  2:1580473/1‑150 (MQ=255)

                                                             gtgtTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGata                                                                                          <  1:4288520/150‑1 (MQ=255)

                                                               gtTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATAtt                                                                                        >  1:3808507/1‑150 (MQ=255)

                                                                ttCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTa                                                                                       <  1:3102333/150‑1 (MQ=255)

                                                                   cagcagGAATATCGCGATTATATGGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAg                                                                                    <  1:1397164/150‑1 (MQ=255)

                                                                      cagGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGaa                                                                                 <  2:4554711/150‑1 (MQ=255)

                                                                        ngAANATCGCGATTATATCGACGTCNTGAAGCTTTTTCAGCTTTTGCCGNGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAAc                                                                               >  1:3998141/2‑150 (MQ=255)

                                                                        ggAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAAc                                                                               >  2:939887/1‑150 (MQ=255)

                                                                          aaTATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAg                                                                             <  2:4876060/150‑1 (MQ=255)

                                                                          aaTATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAg                                                                             >  1:2017794/1‑150 (MQ=255)

                                                                            tatCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGcc                                                                           >  1:5419074/1‑150 (MQ=255)

                                                                              tCGCGATTATGTCGCCGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCGTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCgg                                                                         <  2:1135802/150‑1 (MQ=255)

                                                                              tCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCgg                                                                         <  1:831548/150‑1 (MQ=255)

                                                                               cgcgATTATATGGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGga                                                                        <  1:3280916/150‑1 (MQ=255)

                                                                                gcgATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGgag                                                                       <  1:647362/150‑1 (MQ=255)

                                                                                gcgATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGgag                                                                       >  1:372663/1‑150 (MQ=255)

                                                                                 cgATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGgaga                                                                      <  2:4166223/150‑1 (MQ=255)

                                                                                  gATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCgaagaa                                                                     >  2:5319760/1‑150 (MQ=255)

                                                                                    ttATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATa                                                                   <  2:5203480/150‑1 (MQ=255)

                                                                                    ttATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATa                                                                   <  1:3599636/150‑1 (MQ=255)

                                                                                       tatCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAGGCTGCTTGCTCAGCTTGGAGAAACGGCAAGAGATGTTTTTCATCTGAGGATATTAAAGGAAAGAACCGCAGAATAAac                                                                >  1:1022417/1‑150 (MQ=255)

                                                                                       tatCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAac                                                                <  1:3803548/150‑1 (MQ=255)

                                                                                        ttCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAaca                                                               >  1:4737960/2‑150 (MQ=255)

                                                                                        atCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGTGGATATTAAAGGAAACAGCCGAAGAATAAaca                                                               >  2:1512972/1‑150 (MQ=255)

                                                                                         tCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAacac                                                              >  1:4098552/1‑150 (MQ=255)

                                                                                           gACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTc                                                            >  1:3007101/1‑150 (MQ=255)

                                                                                            tcgtcgtGAAGTTTTCTCAGCTTTTGCCGGCCGTAGGAGCGGCAGTCGGCGGAATTCCCAATTATAAGTTGCTTGCCCAGCCTGGAGAAATGGCAATACATGTTTTTCAACTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTcc                                                           <  2:5848630/149‑1 (MQ=255)

                                                                                            aCGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAACCGGAGAATAAACACTcc                                                           <  1:4422477/150‑1 (MQ=255)

                                                                                              gtcgtGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCgc                                                         >  2:5401095/1‑149 (MQ=255)

                                                                                               tcgtGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGc                                                        <  2:4747094/150‑1 (MQ=255)

                                                                                                 gtGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTg                                                      <  2:3034316/150‑1 (MQ=255)

                                                                                                 gtGAAGCTTTTGCAGCTTTTGCCGAGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCAACTGAGGATATTAAAGGAAACAGCCGGAGAAAAAACACtgctgcag                                                      >  2:3974424/1‑143 (MQ=255)

                                                                                                  tGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGt                                                     >  2:4278801/1‑150 (MQ=255)

                                                                                                  tGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGAATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGt                                                     >  2:1189851/1‑150 (MQ=255)

                                                                                                    aaGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGttt                                                   <  2:2332498/150‑1 (MQ=255)

                                                                                                     aGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAAAAAACACTCCGGCTGtttt                                                  <  1:4387720/150‑1 (MQ=255)

                                                                                                          tttCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTtat                                             <  2:2970483/150‑1 (MQ=255)

                                                                                                                cTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTCTTTTTATAGCtga                                       >  1:898753/1‑150 (MQ=255)

                                                                                                                 ttttGCCGGGCGAAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCtgat                                      <  1:205792/150‑1 (MQ=255)

                                                                                                                  tttGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCtgatg                                     >  2:765464/1‑150 (MQ=255)

                                                                                                                      ccGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAg                                 <  2:2520923/150‑1 (MQ=255)

                                                                                                                          gCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGa                             <  1:2751702/150‑1 (MQ=255)

                                                                                                                           cGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGAt                            <  2:3757019/150‑1 (MQ=255)

                                                                                                                            gTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATc                           <  1:4079089/150‑1 (MQ=255)

                                                                                                                             tAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCg                          <  2:1919014/150‑1 (MQ=255)

                                                                                                                              aGGAGCGGCAGTCGGCGGAATTGTCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGc                         <  1:110503/150‑1 (MQ=255)

                                                                                                                                gAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAg                       >  2:3237316/1‑150 (MQ=255)

                                                                                                                                 aGCGGCAGACGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCCTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAgc                      <  2:2835737/150‑1 (MQ=255)

                                                                                                                                           ggcggAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAgg            >  1:5722157/1‑150 (MQ=255)

                                                                                                                                             cggAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGAc          >  1:3901532/1‑150 (MQ=255)

                                                                                                                                              ggAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGAcc         >  1:2493910/1‑150 (MQ=255)

                                                                                                                                                aaTTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCtt       >  1:3213755/1‑150 (MQ=255)

                                                                                                                                                  ttGTCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCTTgg     <  1:519610/150‑1 (MQ=255)

                                                                                                                                                  ttGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCTTgg     >  2:749857/1‑150 (MQ=255)

                                                                                                                                                     ccAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCTTGGCCg  >  1:5590066/1‑150 (MQ=255)

TAAGTCGCTTTTTTCTGTCATCGAAAATTGGGAGACGGAGAAAAAGAGCATCGACTGGAGAGTGTTCCAGCAGGAATATCGCGATTATATCGACGTCGTGAAGCTTTTTCAGCTTTTGCCGGGCGTAGGAGCGGCAGTCGGCGGAATTGCCAATTATAAGCTGCTTGCTCAGCTTGGAGAAACGGCAAGACATGTTTTTCATCTGAGGATATTAAAGGAAACAGCCGGAGAATAAACACTCCGGCTGTTTTTTTATAGCTGATGATAGGTGATCGCAGCGCCGGCAAGGACCTTGGCCG  >  BS168_reference_genome/1079898‑1080196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`