BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,248,775	A→T	21.8%	K37N (AAA→AAT)	cotO →	spore outer coat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,248,775	0	A	T	21.8%	596.0 / 146.8	252	K37N (AAA→AAT)	cotO	spore outer coat protein
Reads supporting (aligned to +/- strand): ref base A (104/93); new base T (32/23); total (136/116)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 270 total aligned reads displayed.

CTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAACACAAGAGGCGAG  >  BS168_reference_genome/1248626‑1248924

   tCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAataga                                                                                                                                                    <  2:4810316/150‑1 (MQ=255)

     tGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAatagaaa                                                                                                                                                  <  2:2630545/150‑1 (MQ=255)

     tGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAaaagaaa                                                                                                                                                  >  2:5129054/1‑150 (MQ=255)

        cATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGc                                                                                                                                               <  1:2826372/150‑1 (MQ=255)

          tataGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAGCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCNATGCAGGAGATTGTCATCAAAAGAAAGGCCg                                                                                                                                             >  1:1505905/1‑150 (MQ=255)

           atagatagAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGa                                                                                                                                            >  2:3774548/1‑150 (MQ=255)

           atagatagAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGAATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGa                                                                                                                                            >  2:5308702/1‑150 (MQ=255)

            tagatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGAc                                                                                                                                           >  2:2080201/1‑150 (MQ=255)

             agatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACa                                                                                                                                          >  1:4311713/1‑150 (MQ=255)

             agatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACa                                                                                                                                          >  2:1915744/1‑150 (MQ=255)

               atagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAACGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACaaa                                                                                                                                        >  2:5673403/1‑150 (MQ=255)

                 agaATATGCCACAATCGGAGGGATGAAGATGTCTATAAAGAAAAGAAACGTGGATCAAAAACCGCTCATGGATATTGTACAGCCAGATTATGCAGCAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAAcc                                                                                                                                      <  1:5517189/150‑1 (MQ=255)

                 agaATATGACACAATCGGGGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAAcc                                                                                                                                      >  1:2335338/1‑150 (MQ=255)

                 agaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAAcc                                                                                                                                      <  2:3077988/150‑1 (MQ=255)

                   aaTATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCg                                                                                                                                    >  2:2520498/1‑150 (MQ=255)

                    atatGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGc                                                                                                                                   >  2:2401842/1‑150 (MQ=255)

                     tatGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCt                                                                                                                                  >  2:2865582/1‑150 (MQ=255)

                     tatGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCt                                                                                                                                  >  2:2204266/1‑150 (MQ=255)

                      atGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTc                                                                                                                                 <  1:2516123/150‑1 (MQ=255)

                        gACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCg                                                                                                                               >  2:4138689/1‑150 (MQ=255)

                          cacaATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGcc                                                                                                                             >  1:1803275/1‑150 (MQ=255)

                           acaATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCa                                                                                                                            <  1:1105455/150‑1 (MQ=255)

                            caATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAATTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCaa                                                                                                                           >  1:5203285/1‑150 (MQ=255)

                                 ggAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCt                                                                                                                      >  2:3422968/1‑150 (MQ=255)

                                   aGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGa                                                                                                                    <  2:4543013/150‑1 (MQ=255)

                                   aGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGa                                                                                                                    <  1:4629935/150‑1 (MQ=255)

                                    gggATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAg                                                                                                                   >  2:355537/1‑150 (MQ=255)

                                     ggATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAgg                                                                                                                  >  1:5853098/1‑150 (MQ=255)

                                       aTGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGaa                                                                                                                >  2:3598621/1‑150 (MQ=255)

                                           aGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGc                                                                                                            <  2:2287798/150‑1 (MQ=255)

                                           aGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGc                                                                                                            <  1:2585086/150‑1 (MQ=255)

                                               gTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGc                                                                                                        >  2:1469353/1‑150 (MQ=255)

                                                tCTAATAAGAAAAGAAAGGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCt                                                                                                       >  1:2915367/1‑150 (MQ=255)

                                                tCTAATAAGAAAAGAAACGCGGATAAAAATCGGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCt                                                                                                       <  2:2908764/150‑1 (MQ=255)

                                                tCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCt                                                                                                       >  2:3290896/1‑150 (MQ=255)

                                                   aataaGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTAc                                                                                                    >  2:1656444/1‑150 (MQ=255)

                                                    ataaGAAAAGAAACTCGGATAAAAAACCGCCCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTTCGCCAAAAGCTGAGGAAAAGCCGGCTTAcc                                                                                                   <  1:5484855/150‑1 (MQ=255)

                                                    ataaGAAAAGAAACGCGGATAAAAGACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCAGGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTAcc                                                                                                   >  1:3407197/1‑150 (MQ=255)

                                                    ataaGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTAcc                                                                                                   <  2:387704/150‑1 (MQ=255)

                                                    ataaGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTAcc                                                                                                   <  2:5777723/150‑1 (MQ=255)

                                                     taaGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCa                                                                                                  <  2:798458/150‑1 (MQ=255)

                                                       agaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATg                                                                                                >  2:5842241/1‑150 (MQ=255)

                                                       agaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATg                                                                                                >  1:1182533/1‑150 (MQ=255)

                                                        gaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATga                                                                                               >  2:2468142/1‑150 (MQ=255)

                                                        gaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATga                                                                                               >  1:3520427/1‑150 (MQ=255)

                                                         aaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATgag                                                                                              >  1:2401769/1‑150 (MQ=255)

                                                           aagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGaa                                                                                            <  1:3253856/150‑1 (MQ=255)

                                                           aagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGaa                                                                                            >  2:68720/1‑150 (MQ=255)

                                                             gaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAagc                                                                                          >  2:4759856/1‑150 (MQ=255)

                                                             gaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAagc                                                                                          <  1:5030814/150‑1 (MQ=255)

                                                               aaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAagcag                                                                                        >  1:3619023/1‑150 (MQ=255)

                                                                 cgcgGATAAAAACCCGCCCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGCTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAgcagca                                                                                      <  1:1244137/150‑1 (MQ=255)

                                                                    ggATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCACTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCagga                                                                                   >  2:4477138/1‑150 (MQ=255)

                                                                     gATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCaggag                                                                                  >  2:4189418/1‑150 (MQ=255)

                                                                       tAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAggagga                                                                                >  2:738453/1‑150 (MQ=255)

                                                                        aaaaaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTTTCACCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGaa                                                                               <  2:843842/150‑1 (MQ=255)

                                                                        aaaaaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGaa                                                                               >  1:1027580/1‑150 (MQ=255)

                                                                          aaaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGc                                                                             <  2:4299257/150‑1 (MQ=255)

                                                                           aaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAggc                                                                            <  1:757972/150‑3 (MQ=255)

                                                                             aCCGTTCATGTATATTGTTCGGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTCAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGAAGGAAGCCGt                                                                          >  1:212606/1‑150 (MQ=255)

                                                                              ccGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGCCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTg                                                                         <  2:4978587/150‑1 (MQ=255)

                                                                                gCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGc                                                                       <  1:1398690/150‑1 (MQ=255)

                                                                                  tCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGcac                                                                     <  2:1244235/150‑1 (MQ=255)

                                                                                   cATGTATATTGTTAAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGACCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGAcaca                                                                    <  1:2065485/150‑1 (MQ=255)

                                                                                          atTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTc                                                             <  1:1469353/150‑1 (MQ=255)

                                                                                           ttGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTcc                                                            >  2:1012325/1‑150 (MQ=255)

                                                                                           ttGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTcc                                                            <  1:4591614/150‑1 (MQ=255)

                                                                                            tGTTCAGCCGGATTATGCAGAATCTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAAGAGGAAGCCGTGGCACAGGATGTCCt                                                           >  2:4331581/1‑150 (MQ=255)

                                                                                              ttCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGc                                                         >  1:1546025/1‑150 (MQ=255)

                                                                                                      gATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGAc                                                 <  1:5673403/150‑1 (MQ=255)

                                                                                                      gATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGAc                                                 <  1:3956954/150‑1 (MQ=255)

                                                                                                        ttATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCa                                               >  2:2559889/1‑150 (MQ=255)

                                                                                                        ttATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCa                                               >  2:4272471/1‑150 (MQ=255)

                                                                                                            gCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAg                                           <  2:4075989/150‑1 (MQ=255)

                                                                                                            gCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAg                                           <  2:5412911/150‑1 (MQ=255)

                                                                                                              aGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGGTGTCCTGCAAGAAGACCAAAAGcc                                         >  1:1692952/1‑150 (MQ=255)

                                                                                                              aGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGcc                                         >  2:5373343/1‑150 (MQ=255)

                                                                                                                aaaCTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTg                                       <  1:841208/150‑1 (MQ=255)

                                                                                                                aaaCTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAACCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTg                                       <  1:5306657/150‑1 (MQ=255)

                                                                                                                   cTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAg                                    <  1:3127562/150‑1 (MQ=255)

                                                                                                                    tACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGc                                   <  1:4512858/150‑1 (MQ=255)

                                                                                                                    tACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGc                                   >  1:5122072/1‑150 (MQ=255)

                                                                                                                     aCAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGcc                                  <  1:2548774/150‑1 (MQ=255)

                                                                                                                       aaGGTCATCCATGCAGGAGATTGTCATCAATAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGa                                >  2:4569673/1‑150 (MQ=255)

                                                                                                                       aaGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGTTGTCCTGCAAGAAGACCAAAAACCTGCAGCCGa                                >  2:613608/1‑150 (MQ=255)

                                                                                                                       aaGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGa                                <  1:3795937/150‑1 (MQ=255)

                                                                                                                          gTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAAcc                             <  2:1069623/150‑1 (MQ=255)

                                                                                                                              tCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTg                         <  1:5612274/150‑1 (MQ=255)

                                                                                                                                cATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAg                       <  1:4272488/150‑1 (MQ=255)

                                                                                                                                cATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAg                       <  2:2797717/150‑1 (MQ=255)

                                                                                                                                  tGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTc                     >  1:2775441/1‑150 (MQ=255)

                                                                                                                                    cAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAg                   >  2:3715555/1‑150 (MQ=255)

                                                                                                                                         gaTTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAac              >  1:3796853/1‑150 (MQ=255)

                                                                                                                                         gaTTGACATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAac              <  1:613967/150‑1 (MQ=255)

                                                                                                                                          aTTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAaca             <  1:1915744/150‑1 (MQ=255)

                                                                                                                                          aTTGTCATCAAAAGAAAGGCCAACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAATCAGTGAGTCAGCAAaca             <  1:5151195/150‑1 (MQ=255)

                                                                                                                                                atcaAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAACACAagag       >  1:5558885/1‑150 (MQ=255)

                                                                                                                                                 ccaAAAGAAAGGCCTACAAACCCGCTCCCCCAAAAGTTGAGGAATAGTCGGCTTAGCATGTGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCATCAAGAAGACCAAAAGCCTGCAGCCGAACCAGAGAGTCAGCAAACACAAGAgg      <  2:179010/149‑1 (MQ=255)

                                                                                                                                                    aaagaaagGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAACACAAGAGGCga   >  1:4824055/1‑150 (MQ=255)

                                                                                                                                                     aagaaagGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAACACAAGAGGCgag  <  2:2682318/150‑1 (MQ=255)

CTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGGAGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAGCCTGCAGCCGAACCAGTGAGTCAGCAAACACAAGAGGCGAG  >  BS168_reference_genome/1248626‑1248924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`