BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,524,173	T→G	24.1%	T205P (ACC→CCC)	rnjA ←	ribonuclease J1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,524,173	0	T	G	24.1%	595.7 / 241.5	273	T205P (ACC→CCC)	rnjA	ribonuclease J1
Reads supporting (aligned to +/- strand): ref base T (111/96); new base G (35/31); total (146/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

ACGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCGGGCG  >  BS168_reference_genome/1524025‑1524322

aCGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTa                                                                                                                                                      <  1:1001329/150‑1 (MQ=255)

aCGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTa                                                                                                                                                      <  1:1242027/150‑1 (MQ=255)

aCGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTa                                                                                                                                                      >  2:1298312/1‑150 (MQ=255)

 cGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTaa                                                                                                                                                     <  1:4140437/150‑1 (MQ=255)

 cGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGaa                                                                                                                                                     <  1:3371086/150‑1 (MQ=255)

  ggCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGACATGAATGCTTTCTCCTACACGGAGCTCAGACATGGTaaa                                                                                                                                                    >  2:27537/1‑150 (MQ=255)

     aaCTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTc                                                                                                                                                 >  2:4307573/1‑150 (MQ=255)

     aaCTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTc                                                                                                                                                 >  2:1574815/1‑150 (MQ=255)

       cTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCg                                                                                                                                               >  2:2518643/1‑150 (MQ=255)

         tttCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGa                                                                                                                                             >  2:836470/1‑150 (MQ=255)

          ttcttcCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGAt                                                                                                                                            <  2:5231966/150‑1 (MQ=255)

                 aTTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACt                                                                                                                                     <  2:2212694/150‑1 (MQ=255)

                 aTTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACt                                                                                                                                     <  2:4420402/150‑1 (MQ=255)

                  ttttGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTg                                                                                                                                    <  1:2191640/150‑1 (MQ=255)

                    ttGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGtt                                                                                                                                  <  2:1024212/150‑1 (MQ=255)

                     tGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGttt                                                                                                                                 <  2:3764224/150‑1 (MQ=255)

                        aCAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTtgtg                                                                                                                              <  1:331749/150‑1 (MQ=255)

                         cagcagCTTCAATTACTTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGAGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGc                                                                                                                             <  1:2020241/150‑1 (MQ=255)

                         cagcagCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGc                                                                                                                             <  1:190621/150‑1 (MQ=255)

                              gcTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCt                                                                                                                        >  2:176943/1‑150 (MQ=255)

                                 tCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGaa                                                                                                                     >  2:1206287/1‑150 (MQ=255)

                                       aCCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAgg                                                                                                               <  1:1448865/150‑1 (MQ=255)

                                       aCCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAgg                                                                                                               <  1:4484126/150‑1 (MQ=255)

                                         ctgctgCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGca                                                                                                             <  1:4082973/150‑1 (MQ=255)

                                           gctgcAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGcaca                                                                                                           >  2:5028549/1‑150 (MQ=255)

                                              ncAANCGGTGAATATTCGACGCAAANGTGGCGAAGATAATTCGGCCGTGNACCTTGCGGNAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACaaaa                                                                                                        >  1:4003290/2‑150 (MQ=255)

                                               cAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAAc                                                                                                       <  1:640284/150‑1 (MQ=255)

                                                aaGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACg                                                                                                      <  2:4765439/150‑1 (MQ=255)

                                                aaGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACg                                                                                                      >  1:5633595/1‑150 (MQ=255)

                                                   cGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCct                                                                                                   >  1:5781885/1‑150 (MQ=255)

                                                   cGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCct                                                                                                   >  1:2336762/1‑150 (MQ=255)

                                                      tGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGTGGAAAATGTCATGAATGCTTTCTCCTACACGGTGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCcttct                                                                                                >  2:1694496/1‑150 (MQ=255)

                                                      tGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCcttct                                                                                                >  1:4268154/1‑150 (MQ=255)

                                                      tGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCcttct                                                                                                <  1:3095333/150‑1 (MQ=255)

                                                       gAATATTCGTCGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGTGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAGCGCcttctt                                                                                               >  2:5518331/1‑150 (MQ=255)

                                                       gAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCcttctt                                                                                               <  1:5103333/150‑1 (MQ=255)

                                                         atatTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTa                                                                                             <  2:2129774/150‑1 (MQ=255)

                                                           atTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTAcc                                                                                           <  1:5698952/150‑1 (MQ=255)

                                                              cGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGAt                                                                                        <  1:3659493/150‑1 (MQ=255)

                                                              cGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGAt                                                                                        <  1:4376103/150‑1 (MQ=255)

                                                              cGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGAt                                                                                        >  2:5360162/1‑150 (MQ=255)

                                                              cGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGAt                                                                                        >  2:1124426/1‑150 (MQ=255)

                                                               gACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGAtt                                                                                       >  2:2330953/1‑150 (MQ=255)

                                                                 cGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTc                                                                                     <  1:2124620/150‑1 (MQ=255)

                                                                  gCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCg                                                                                    <  1:5823695/150‑1 (MQ=255)

                                                                  gCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCg                                                                                    >  1:150775/1‑150 (MQ=255)

                                                                   cAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTTAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCgg                                                                                   >  1:601648/1‑150 (MQ=255)

                                                                        gtgGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGGCATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCAtt                                                                              >  2:47020/1‑150 (MQ=255)

                                                                           gCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTc                                                                           <  1:1038892/150‑1 (MQ=255)

                                                                            cGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCg                                                                          <  2:1906305/150‑1 (MQ=255)

                                                                            cGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCg                                                                          <  1:2609821/150‑1 (MQ=255)

                                                                              aaGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGtt                                                                        <  2:3378705/150‑1 (MQ=255)

                                                                                 aTAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAg                                                                     >  1:5238835/1‑150 (MQ=255)

                                                                                 aTAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAg                                                                     >  1:238780/1‑150 (MQ=255)

                                                                                  tAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAACACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGt                                                                    <  2:2943943/150‑1 (MQ=255)

                                                                                   aaTTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGtt                                                                   >  1:1175306/1‑150 (MQ=255)

                                                                                    aTTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGttt                                                                  <  1:4444963/150‑1 (MQ=255)

                                                                                    aTTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGttt                                                                  <  1:1759302/150‑1 (MQ=255)

                                                                                       cGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAGACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGcc                                                               <  2:4360065/150‑1 (MQ=255)

                                                                                        ggCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCg                                                              <  1:3153845/150‑1 (MQ=255)

                                                                                            gTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTc                                                          >  2:1426290/1‑150 (MQ=255)

                                                                                            gTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTc                                                          >  2:617267/1‑150 (MQ=255)

                                                                                             tCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCg                                                         <  1:5526657/150‑1 (MQ=255)

                                                                                                 ccTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGa                                                     <  1:5265251/150‑1 (MQ=255)

                                                                                                  cTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGAc                                                    >  2:898973/1‑150 (MQ=255)

                                                                                                    tGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTg                                                  >  2:4853169/1‑150 (MQ=255)

                                                                                                    tGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTg                                                  >  1:3004725/1‑150 (MQ=255)

                                                                                                    tGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTg                                                  >  1:3727711/1‑150 (MQ=255)

                                                                                                      cGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGa                                                >  1:473576/1‑150 (MQ=255)

                                                                                                         aaaaTGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACAGGAGTa                                             <  1:5746708/150‑1 (MQ=255)

                                                                                                          aaaTGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTaa                                            >  2:4801748/1‑150 (MQ=255)

                                                                                                          aaaTGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTaa                                            <  1:2040791/150‑1 (MQ=255)

                                                                                                               tCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCg                                       >  2:68934/1‑150 (MQ=255)

                                                                                                               tCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCg                                       <  1:5573106/150‑1 (MQ=255)

                                                                                                               tCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCg                                       <  2:5633595/150‑1 (MQ=255)

                                                                                                                  tGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAt                                    >  2:3125291/1‑150 (MQ=255)

                                                                                                                  tGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAt                                    >  1:3899045/1‑150 (MQ=255)

                                                                                                                   gAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAtt                                   >  1:1106152/1‑150 (MQ=255)

                                                                                                                   gAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAtt                                   >  1:2829192/1‑150 (MQ=255)

                                                                                                                   gAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAtt                                   <  1:709230/150‑1 (MQ=255)

                                                                                                                   gAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAAtt                                   <  1:3561020/150‑1 (MQ=255)

                                                                                                                       gCTTTCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGaa                               >  2:2038099/1‑150 (MQ=255)

                                                                                                                        cTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGaaa                              >  2:2364257/1‑150 (MQ=255)

                                                                                                                         tttCTCCTACACGGCGCTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAAt                             >  2:415004/1‑150 (MQ=255)

                                                                                                                             tcCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATcccc                         >  1:3277986/1‑150 (MQ=255)

                                                                                                                               cTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAg                       >  1:1587168/1‑150 (MQ=255)

                                                                                                                                tACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGt                      <  1:836470/150‑1 (MQ=255)

                                                                                                                                 acacGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCGCAGTa                     >  1:1331205/1‑150 (MQ=255)

                                                                                                                                      gcgcTCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTAc                >  2:3542778/1‑150 (MQ=255)

                                                                                                                                         cTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGAt             >  2:572159/1‑150 (MQ=255)

                                                                                                                                          tCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATg            >  2:64495/1‑150 (MQ=255)

                                                                                                                                          tCAGACATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATg            >  2:3809841/1‑150 (MQ=255)

                                                                                                                                           cAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGt           <  1:1626512/150‑1 (MQ=255)

                                                                                                                                           cAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGt           >  2:2832145/1‑150 (MQ=255)

                                                                                                                                               cATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTcc       >  2:4006596/1‑150 (MQ=255)

                                                                                                                                               cATGGGAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTcc       >  2:4526512/1‑150 (MQ=255)

                                                                                                                                                 tGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCgg     >  1:769005/1‑150 (MQ=255)

                                                                                                                                                 tGGTAAACACCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCgg     <  2:4601667/150‑1 (MQ=255)

                                                                                                                                                  ggTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCggg    >  2:1641155/1‑150 (MQ=255)

                                                                                                                                                  ggTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCggg    >  1:343491/1‑150 (MQ=255)

ACGGCAACTTTTCTTCCATTTTGTACAGCAGCTTCAATTACCTGCTGCAAGCGGTGAATATTCGACGCAAATGTGGCGAAGATAATTCGGCCGTCCACCTTGCGGAAAATGTCATGAATGCTTTCTCCTACACGGCGCTCAGACATGGTAAACTCCGGATTTTCACTGTTTGTGCTGTCTGAAAGAAGGCACAAAACGCCTTCTTTACCGATTTCGGCCATTTTCGTTAAGTTTGCCGGCTCGCCGACTGGAGTAAAGTCGAATTTGAAATCCCCAGTATGTACGATGTTTCCGGGCG  >  BS168_reference_genome/1524025‑1524322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`