BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,544,043	C→T	18.4%	F76F (TTC→TTT)	ylaB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,544,043	0	C	T	18.4%	653.1 / 94.6	228	F76F (TTC→TTT)	ylaB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (110/76); new base T (28/14); total (138/90)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 235 total aligned reads displayed.

ATGGAATGGATGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTAGCG  >  BS168_reference_genome/1543894‑1544192

atggaatggaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTc                                                                                                                                                       >  1:4362087/1‑150 (MQ=255)

atggaatggaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTc                                                                                                                                                       <  2:416475/150‑1 (MQ=255)

    aatggaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCAttt                                                                                                                                                   <  2:1591730/150‑1 (MQ=255)

     atggaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCAtttt                                                                                                                                                  <  2:3710190/150‑1 (MQ=255)

       ggaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTtttattttat                                                                                                                                                >  1:58075/1‑150 (MQ=255)

        gaTGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTAtt                                                                                                                                               >  2:4289407/1‑150 (MQ=255)

          tGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTgg                                                                                                                                             >  2:5626582/1‑150 (MQ=255)

           gAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGc                                                                                                                                            <  2:2751602/150‑1 (MQ=255)

                  cATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTgg                                                                                                                                     >  1:1956853/1‑150 (MQ=255)

                   aTGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGa                                                                                                                                    <  2:414208/150‑1 (MQ=255)

                    tGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGAt                                                                                                                                   >  1:913112/1‑150 (MQ=255)

                    tGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGAt                                                                                                                                   >  1:643602/1‑150 (MQ=255)

                    tGAATCCCTATTTCAAAAGAATGCGGAAGACATAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGAt                                                                                                                                   <  2:75073/150‑1 (MQ=255)

                      aaTCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGa                                                                                                                                 >  1:5202989/1‑150 (MQ=255)

                      aaTCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGa                                                                                                                                 >  2:5588385/1‑150 (MQ=255)

                          ccTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGgt                                                                                                                             <  2:3852939/150‑1 (MQ=255)

                          ccTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGgt                                                                                                                             <  2:3513063/150‑1 (MQ=255)

                               ttCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGt                                                                                                                        >  1:1921450/1‑150 (MQ=255)

                                tCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGtt                                                                                                                       <  2:5327057/150‑1 (MQ=255)

                                 cAAAAGAATGCAGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCAGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATAACGGTGTGGttt                                                                                                                      >  1:1889254/1‑150 (MQ=255)

                                  aaaaGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGtttt                                                                                                                     <  2:1836475/150‑1 (MQ=255)

                                   aaaGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTa                                                                                                                    <  2:788963/150‑1 (MQ=255)

                                     aGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTAtt                                                                                                                  <  2:643602/150‑1 (MQ=255)

                                      gAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTAttt                                                                                                                 <  2:4203639/150‑1 (MQ=255)

                                       aaTGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTAtttt                                                                                                                <  2:1712287/150‑1 (MQ=255)

                                        aTGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTAttttt                                                                                                               >  1:3466843/1‑150 (MQ=255)

                                         tGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTa                                                                                                              >  1:1141284/1‑150 (MQ=255)

                                         tGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTa                                                                                                              <  2:3330346/150‑1 (MQ=255)

                                          gCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTaa                                                                                                             >  2:4344470/1‑150 (MQ=255)

                                             gAACACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGa                                                                                                          >  1:2483482/1‑150 (MQ=255)

                                               aGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTATTATTTTTAATGAAg                                                                                                        >  1:2925172/1‑150 (MQ=255)

                                                    tAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAg                                                                                                   >  2:5525144/1‑150 (MQ=255)

                                                     aaaGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAgg                                                                                                  >  2:4905249/1‑150 (MQ=255)

                                                      aaGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAggg                                                                                                 >  1:4312434/1‑150 (MQ=255)

                                                       agagTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGa                                                                                                >  1:2096401/1‑150 (MQ=255)

                                                        gagTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGAt                                                                                               >  1:47464/1‑150 (MQ=255)

                                                         agTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGAtt                                                                                              >  1:470177/1‑150 (MQ=255)

                                                          gTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTc                                                                                             >  1:4774688/1‑150 (MQ=255)

                                                           tAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTcc                                                                                            >  2:2675383/1‑150 (MQ=255)

                                                            aaaaCTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCa                                                                                           >  2:1616379/1‑150 (MQ=255)

                                                             aaaCTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCAt                                                                                          >  2:2789517/1‑150 (MQ=255)

                                                              aaCTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGGCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCAtt                                                                                         >  1:4866409/1‑150 (MQ=255)

                                                                   gTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGa                                                                                    >  1:5456022/1‑150 (MQ=255)

                                                                     ttAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACt                                                                                  <  2:2086907/150‑1 (MQ=255)

                                                                        aaGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGt                                                                               <  2:5067076/150‑1 (MQ=255)

                                                                         agagAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTa                                                                              >  1:5852659/1‑150 (MQ=255)

                                                                           agaAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATc                                                                            <  2:2765733/150‑1 (MQ=255)

                                                                            gaAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCg                                                                           >  2:1221291/1‑150 (MQ=255)

                                                                               gTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCa                                                                        >  1:4529662/1‑150 (MQ=255)

                                                                               gTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCa                                                                        >  2:395097/1‑150 (MQ=255)

                                                                               gTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCa                                                                        >  1:4057522/1‑150 (MQ=255)

                                                                               gTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCa                                                                        <  2:4362087/150‑1 (MQ=255)

                                                                                  ccGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATCAAGCATAGGGATTCCATTGAGGCCTTGTATCGGCAGta                                                                     <  2:3386682/150‑1 (MQ=255)

                                                                                     gTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGtatta                                                                  >  2:2267269/1‑150 (MQ=255)

                                                                                     gTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGtatta                                                                  >  2:5218198/1‑150 (MQ=255)

                                                                                     gTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATACCATTGAGGACTTGTATCGGCAGtatta                                                                  <  2:1889254/150‑1 (MQ=255)

                                                                                          gaagaagaaAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAg                                                             <  2:1309592/150‑1 (MQ=255)

                                                                                           aagaagaaAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGa                                                            >  2:846711/1‑150 (MQ=255)

                                                                                             gaagaaAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGTGGACTTGTATCGGCAGTATTATCAAGaaa                                                          >  2:4840050/1‑150 (MQ=255)

                                                                                              aagaaAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAAt                                                         <  2:3431800/150‑1 (MQ=255)

                                                                                              aagaaAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAAt                                                         <  2:323895/150‑1 (MQ=255)

                                                                                                 aaaGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAAtttt                                                      >  1:1674941/1‑150 (MQ=255)

                                                                                                 aaaGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAAtttt                                                      >  2:2952140/1‑150 (MQ=255)

                                                                                                   aGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTaa                                                    >  1:117886/1‑150 (MQ=255)

                                                                                                   aGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTaa                                                    >  1:5331825/1‑150 (MQ=255)

                                                                                                    gCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTaaa                                                   <  2:5202989/150‑1 (MQ=255)

                                                                                                       aaaTCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAatta                                                >  1:5104152/1‑150 (MQ=255)

                                                                                                       aaaTCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAatta                                                >  2:4397035/1‑150 (MQ=255)

                                                                                                        aaTCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAattat                                               >  2:3121925/1‑150 (MQ=255)

                                                                                                        aaTCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAattat                                               <  2:4750552/150‑1 (MQ=255)

                                                                                                         aTCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAattatt                                              >  1:1546162/1‑150 (MQ=255)

                                                                                                            aGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAAttatttat                                           >  1:1304542/1‑150 (MQ=255)

                                                                                                            aGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAAttatttat                                           >  2:4600219/1‑150 (MQ=255)

                                                                                                               tatatGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCa                                        >  2:1910135/1‑150 (MQ=255)

                                                                                                               tatatGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCa                                        <  2:1932290/150‑1 (MQ=255)

                                                                                                                   tGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAg                                    >  1:3182988/1‑150 (MQ=255)

                                                                                                                    gAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAgg                                   >  2:5375556/1‑150 (MQ=255)

                                                                                                                       aGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACt                                >  1:499122/1‑150 (MQ=255)

                                                                                                                        gTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACtc                               >  1:278740/1‑150 (MQ=255)

                                                                                                                        gTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAAGTTTAAATTATTTATTCAGAAGGACtc                               >  2:505076/1‑150 (MQ=255)

                                                                                                                             ccTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTcatcat                          <  2:5474479/150‑1 (MQ=255)

                                                                                                                             ccTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGCCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTcatcat                          >  2:3306653/1‑150 (MQ=255)

                                                                                                                              cTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCatcatc                         <  2:2451784/150‑1 (MQ=255)

                                                                                                                                 tCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTg                      <  2:117886/150‑1 (MQ=255)

                                                                                                                                  cTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGa                     <  1:2675383/150‑1 (MQ=255)

                                                                                                                                   ttCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGaa                    >  1:37643/1‑150 (MQ=255)

                                                                                                                                        tGTATATCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGc               >  1:2258483/1‑150 (MQ=255)

                                                                                                                                         gTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGcc              >  2:4256094/1‑150 (MQ=255)

                                                                                                                                          tataTCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCa             >  1:3272749/1‑150 (MQ=255)

                                                                                                                                           atatCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCaa            >  1:4185992/1‑150 (MQ=255)

                                                                                                                                           atatCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCaa            <  2:2351807/150‑1 (MQ=255)

                                                                                                                                            tatCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAg           <  2:1384857/150‑1 (MQ=255)

                                                                                                                                             atCTGTTTTATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAgg          >  2:5754853/1‑150 (MQ=255)

                                                                                                                                               cTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGAc        <  1:3319979/150‑1 (MQ=255)

                                                                                                                                                tGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACt       <  2:5852659/150‑1 (MQ=255)

                                                                                                                                                tGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACt       <  1:5525144/150‑1 (MQ=255)

                                                                                                                                                  tttCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTa     <  1:3240937/150‑1 (MQ=255)

                                                                                                                                                    tCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTAgc   <  2:4794380/150‑1 (MQ=255)

                                                                                                                                                    tCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTAgc   <  2:4511145/150‑1 (MQ=255)

                                                                                                                                                    tCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTAgc   >  2:1537749/1‑150 (MQ=255)

ATGGAATGGATGAAACAGCATGAATCCCTATTTCAAAAGAATGCGGAAGACTTAAAGAGTAAAACTTGTTTAAAGAGAAGTCCCGGTGCTGAAGAAGAAAGCCAAATCAGATATATGAAAGTATACCTGTCTTCCATGTATATCTGTTTCATTTTATTGGCCATTTGGATGACGGTGTGGTTTTATTTTTAATGAAGCATAGGGATTCCATTGAGGACTTGTATCGGCAGTATTATCAAGAAATTTTAAATTATTTATTCAGAAGGACTCATCATCTTGAAACAGCCAAGGACTTAGCG  >  BS168_reference_genome/1543894‑1544192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`