Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,579,791 (A)7→6 coding (79/132 nt) U712_18380 → Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,579,7850A.98.6% 1039.2 / 6.6 212coding (73/132 nt)U712_18380Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base . (97/112);  total (100/112)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

Only 100 of 237 total aligned reads displayed.

ACACCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTCGTGCGTA  >  CP006881/3579637‑3579930
                                                                                                                                                    |                                                                                                                                                 
acacCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAGTCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                                  <  2:1328951/150‑1 (MQ=255)
acacCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                                  >  2:398983/1‑150 (MQ=255)
 cacCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaa                                                                                                                                                 <  2:2837838/150‑1 (MQ=255)
  acCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaa                                                                                                                                                >  2:2729666/1‑150 (MQ=255)
   ccAAATCCTGAAGCAAGCATACAATAGGCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCACCAAAACGGATGAAGTCGGTATCAATCCGAAACCCCACCTTTTATTTCGGGGGTCTGCATGCaaaaa                                                                                                                                               >  1:3140537/1‑150 (MQ=255)
   ccAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaa                                                                                                                                               <  1:1911983/150‑1 (MQ=255)
    cAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaa                                                                                                                                              <  1:690496/150‑1 (MQ=255)
      aaTCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                                            <  1:1258968/150‑3 (MQ=255)
       gtCCTGAAGCAAGCATACAAAAGCCATCTCTAGTGGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAGAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGCCTGCATGCaaaaaacac                                                                                                                                           <  1:1477718/149‑4 (MQ=255)
         ccTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAacacac                                                                                                                                        <  2:2774938/150‑1 (MQ=255)
          cTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCACCAAAGCAACGCTGTTACACCGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAACCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACAcc                                                                                                                                       <  1:812033/150‑1 (MQ=255)
            gAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCg                                                                                                                                     <  2:1538974/150‑1 (MQ=255)
             aagcaagcATCCAATAGACACCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAACCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCgg                                                                                                                                    <  1:3615477/150‑1 (MQ=255)
                  agcaTACAATAGACATCTCTAGTAGAGGAAAACAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGt                                                                                                                               <  2:3321331/150‑1 (MQ=255)
                           tAGCCATCTCAAGTAGAGGAAACCAACAAAGCAACGCTGTTACCCAGAAAAGAATATCAAAAAAACGGGTGAAGTCGGAACCAATCCGACACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAt                                                                                                                      <  1:713330/150‑1 (MQ=255)
                            aGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtc                                                                                                                     <  2:2192522/150‑1 (MQ=255)
                             gACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtct                                                                                                                    <  1:2734833/150‑1 (MQ=255)
                             gACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGGCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtct                                                                                                                    >  2:1491873/1‑150 (MQ=255)
                              aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAATCACACCCGGATCGTCTGGAAAAtctc                                                                                                                   >  2:735643/1‑150 (MQ=255)
                              aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                                   <  2:1396881/150‑1 (MQ=255)
                               cATCTCTAGTAGAGGAAATCGACAAAGCCACGCTGTTACCCAGAAATGAATACCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACCCCCGGATCGTCTGGAAAATCTca                                                                                                                  <  1:1810988/150‑1 (MQ=255)
                               cATCTCTAGTAGAGGAAATCACCAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTca                                                                                                                  <  2:1741948/150‑1 (MQ=255)
                               cATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTca                                                                                                                  <  2:2961346/150‑1 (MQ=255)
                                aTCTCTGGTAGAGGACATACACAAAGCCACCCCTTTACCCAGTAATGAATAACAAAAAAACGGATGAAGGCGGAAACAATCCGAAACCGCACTTTTTATTCCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                                 <  1:2992961/150‑1 (MQ=255)
                                aTCTCTAGTAGAGGAAACCAACAAAGCACCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaa                                                                                                                 <  2:1175942/150‑1 (MQ=255)
                                      agtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATcc                                                                                                           <  2:1513199/150‑1 (MQ=255)
                                        tagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCgg                                                                                                         <  2:459468/150‑1 (MQ=255)
                                          gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGt                                                                                                       >  2:1687495/1‑150 (MQ=255)
                                           agGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTg                                                                                                      <  2:8308/150‑1 (MQ=255)
                                             gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCa                                                                                                    <  1:1669006/150‑1 (MQ=255)
                                             gAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCa                                                                                                    <  1:389481/150‑1 (MQ=255)
                                              aaaTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAg                                                                                                   <  1:227866/150‑1 (MQ=255)
                                               aaTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAgg                                                                                                  >  2:2469400/1‑149 (MQ=255)
                                                aTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAt                                                                                                 <  1:1697061/150‑1 (MQ=255)
                                                 tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAATAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtc                                                                                                <  2:2401764/150‑1 (MQ=255)
                                                  caccaaAGCAACGCTGTTACCCAGAAATGAAGTTCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCCCTTTTCATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCACAACAATCCGGGTGCAGAtct                                                                                               <  2:2935239/147‑1 (MQ=255)
                                                   aacaaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtctt                                                                                              >  2:1610536/1‑150 (MQ=255)
                                                   aacaaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGAACTTTGTATTTCGGGGGTCTGCATGC‑AAAAAACACACCCCGATCGTCAGGAAAATCTCAACAATCCGGGTGCAGAtctt                                                                                              >  2:3872109/1‑150 (MQ=255)
                                                         gCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTaa                                                                                        <  2:349703/150‑1 (MQ=255)
                                                         gCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTaa                                                                                        >  2:2029077/1‑150 (MQ=255)
                                                          cAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAAt                                                                                       <  2:3543810/150‑1 (MQ=255)
                                                           aaCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATa                                                                                      <  1:428729/150‑1 (MQ=255)
                                                            aCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATAc                                                                                     <  1:3038905/150‑1 (MQ=255)
                                                             cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTGTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACa                                                                                    <  1:1891229/150‑1 (MQ=255)
                                                              gCTGTTACCCAGGAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAGTCTCAACAATCCGGGTGCAGACCTCCTTTAATACAt                                                                                   >  2:665143/1‑150 (MQ=255)
                                                              gCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAt                                                                                   >  2:3131492/1‑150 (MQ=255)
                                                              gCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGACGGAATCACTCCGAAACCGCACTTTTTATTTCGGATGCCTGCATGTAAAAAAA‑ACAACCGGATCGTCTCGTAAATATCAACAATCCGGGTGCAGATCTTCTTTTATACAt                                                                                   >  2:1806229/1‑150 (MQ=255)
                                                               cTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                                  >  2:2985357/1‑150 (MQ=255)
                                                               cTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                                  <  1:3345814/150‑1 (MQ=255)
                                                               cTGTTACCCAGAAATGAATAGCAAAAAATCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                                  <  2:3659301/150‑1 (MQ=255)
                                                                tGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttt                                                                                 <  1:938502/150‑1 (MQ=255)
                                                                  ttACCCAGAAATGAATATCAAAAAAACGGATGAAGCCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttt                                                                               <  2:362932/150‑1 (MQ=255)
                                                                   tACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGGTCGTCTGGGAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttt                                                                               >  2:1011530/1‑150 (MQ=255)
                                                                     cccAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTg                                                                            <  2:2656980/150‑1 (MQ=255)
                                                                          aaaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtct                                                                       <  2:1958600/150‑1 (MQ=255)
                                                                           aaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtctg                                                                      >  2:3787392/1‑150 (MQ=255)
                                                                           aaTGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtctg                                                                      >  2:3163630/1‑150 (MQ=255)
                                                                               aaTATCAAAAAAACGGATGAAGACGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCtt                                                                  <  2:200466/150‑1 (MQ=255)
                                                                                atatCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTc                                                                 >  2:3949592/1‑150 (MQ=255)
                                                                                 tatCAAAAAAACGGATGAAGTCGGTATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCGTGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGACCTTCTTTGATACATTTTCTTGCGTCTGtcctcc                                                                >  2:3434338/1‑150 (MQ=255)
                                                                                         aaacccaTGAGGTCGGACCCCAGCCGCAACCGCCTGTTGTATCTCGGGCCTCTGCATGCACAAAACCTC‑CCCCCATCGTGTGGTAAACCATAACGATCCTAGTTCAGATCTTCTTTAATACATGTTTTTGCGTGGGTCTTCCTCCGTCaa                                                        >  2:1633236/7‑150 (MQ=255)
                                                                                          aaCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTCAATACATTTTTTTGCGTCTGTCTTCCTCAGTCaaa                                                       >  2:3464380/1‑150 (MQ=255)
                                                                                          aaCGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCCGGTGTCTGCATGC‑AAAAAACACACCCGGACCGTCTGGAAACTCCCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCCCAGGCaac                                                       >  2:2585939/1‑149 (MQ=255)
                                                                                             ggATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCCCAGTCAAAAAt                                                    >  2:3065104/1‑150 (MQ=255)
                                                                                              gATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAAtt                                                   >  2:588498/1‑150 (MQ=255)
                                                                                                 gAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                                                >  2:2342604/1‑150 (MQ=255)
                                                                                                 gAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                                                >  2:1706644/1‑150 (MQ=255)
                                                                                                   aGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaaca                                              >  2:3573313/1‑150 (MQ=255)
                                                                                                   aGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaaca                                              >  2:1086616/1‑150 (MQ=255)
                                                                                                    gTCGGAAACAATCTGAAACCGCACTTTTTATTTCGGGAGACTGCATGC‑AAAAAACACACCCTGAGAGTCTGGAAAAACTCAACTATCCGGGTGCAGGTCTTCTTTAATACATTTTTCTGCGTCTGTCTTCCTCAGTCAAAAAGAcaacaa                                             >  1:2122373/1‑150 (MQ=255)
                                                                                                       ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAAc                                          <  2:3811369/150‑1 (MQ=255)
                                                                                                       ggAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGGGCAGATCTTCTTTAATTCATTTTTTTGCGTCTGTCTTCCTCACTCAAAAATTCAACAAAAc                                          >  1:1976504/1‑150 (MQ=255)
                                                                                                        gAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                                         >  1:2831784/1‑150 (MQ=255)
                                                                                                         aatcaatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTc                                        >  1:1623941/1‑150 (MQ=255)
                                                                                                          accatcccGAACCCGCTCTTTTTATTCGGGGTGTCTGCAGGC‑AAAAAACCCCCCGGGATCGGCTGGAAAATCCCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTGGCGTCTGCCCCCCCCAGTCAAAAATTCAACAAACCTcc                                       <  2:2436548/144‑1 (MQ=255)
                                                                                                            caatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGc                                     >  1:2341710/1‑150 (MQ=255)
                                                                                                               tcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAAATCAACAAAACCCCGCATc                                  >  1:3220987/1‑150 (MQ=255)
                                                                                                                ccGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAAAAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCg                                 >  1:826936/1‑150 (MQ=255)
                                                                                                                  gAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCa                               >  2:1824293/1‑150 (MQ=255)
                                                                                                                  gAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGGCAAAAATTCAACAAAACTCCGCATCGCa                               >  2:2785251/1‑150 (MQ=255)
                                                                                                                  gAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCCGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCa                               >  2:600971/1‑150 (MQ=255)
                                                                                                                    aaCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACACAACTCCGCATCGCaaa                             >  1:917426/1‑150 (MQ=255)
                                                                                                                     aCCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCaaaa                            >  2:2173587/1‑150 (MQ=255)
                                                                                                                        gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGc                         >  2:516444/1‑150 (MQ=255)
                                                                                                                         cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGAACGTCTGGAAAATCACAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGct                        >  2:2795698/1‑150 (MQ=255)
                                                                                                                            tttttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTTCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCtt                     >  1:3468758/1‑150 (MQ=255)
                                                                                                                            tttttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCtt                     >  2:3606799/1‑150 (MQ=255)
                                                                                                                               ttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAAACCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCt                  >  1:1152075/1‑150 (MQ=255)
                                                                                                                                 aTTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCttt                >  2:3233837/1‑150 (MQ=255)
                                                                                                                                  tttCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTa               <  2:1843844/150‑1 (MQ=255)
                                                                                                                                    tCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTAtt             >  2:2355770/1‑150 (MQ=255)
                                                                                                                                      gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTg           <  2:1932991/150‑1 (MQ=255)
                                                                                                                                      gggTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTCATTTg           >  2:2425632/1‑150 (MQ=255)
                                                                                                                                       ggTGTCTGCATGC‑AAAAAACTCACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTCCTTTAATACTTTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGt          <  2:2543471/150‑1 (MQ=255)
                                                                                                                                        gtgcCTGCATGC‑AGAAACCACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCGTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTc         <  2:1259985/150‑1 (MQ=255)
                                                                                                                                          gtCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTCGt       >  1:2041841/1‑150 (MQ=255)
                                                                                                                                          gtCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTCGt       >  1:2306584/1‑150 (MQ=255)
                                                                                                                                            cTGCATGC‑AAAAAACACACCCGTATCGTCTGGAAATCCTCAGCAATACTGGCGCAGATCATCATTAACGCCGTTTTGTGGGTCTGTCTTCCTCAGTCATAAATCCAACCAAACCCCGCATCGCAAAAGGCTCTTCCTCTATTTGTCGTGc     >  2:3278842/1‑150 (MQ=255)
                                                                                                                                             tgcatgc‑aCAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTCGTGCg    <  2:2691711/150‑1 (MQ=255)
                                                                                                                                             tgcatgc‑aAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAGGGCTCTTCCTTTATTTGTCGTGCg    >  2:3830832/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACACCAAATCCTGAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTTTATTTGTCGTGCGTA  >  CP006881/3579637‑3579930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑