BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,209	+T	intergenic (‑41/‑488)	U712_19205 ← / → U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,205	1	.	T	95.0%	1314.3 / 59.2	381	intergenic (‑37/‑492)	U712_19205/U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand): ref base . (10/9); new base T (184/177); total (195/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 406 total aligned reads displayed.

TGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAC  >  CP006881/3725056‑3725349

 ggTATGTCCGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGCCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑t                                                                                                                                                   <  2:1765690/150‑1 (MQ=255)

  gTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑t‑t                                                                                                                                                 <  1:1496014/150‑1 (MQ=255)

   tATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACGCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑t‑tt                                                                                                                                                <  1:1546749/150‑1 (MQ=255)

    aTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCGCCCAGTTCATCAAAAAGACCATCCACACCCGTAACGATACAAGCAAACGTTTCATAGAAAAACAACCCCCCCCTTCATTTTGGCAGGCAGC‑C‑t‑tct                                                                                                                                               >  2:2455301/1‑148 (MQ=255)

    aTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATACAACCAACCGTTTCATAGAAAAACATCCCTCCCCTTCTTTTTGGCCGGCAGC‑C‑c‑ttt                                                                                                                                               >  1:1969726/1‑150 (MQ=255)

       tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAAAATCCCTCCGCTTCTTTTTGGCGGGCAGG‑C‑t‑ttttag                                                                                                                                            >  1:53604/1‑147 (MQ=255)

        aCGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑t‑ttttagc                                                                                                                                           <  1:832521/150‑5 (MQ=255)

          gCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑agcag                                                                                                                                         >  1:3193739/1‑150 (MQ=255)

             gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCACCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGccc                                                                                                                                      <  2:1632915/150‑1 (MQ=255)

              aGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCg                                                                                                                                     >  2:3756849/1‑150 (MQ=255)

               gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGt                                                                                                                                    <  1:385097/150‑1 (MQ=255)

               gCCGAATAGACCGCGGCAGCCGCAGTTTTCCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGt                                                                                                                                    <  1:3483463/150‑1 (MQ=255)

                ccGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGtt                                                                                                                                   <  1:1339504/150‑1 (MQ=255)

                 cGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCCTCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGGTTCATAGAAAAACAACCCTCCGCCTCCTTTTGGCAGGCAGC‑CTT‑TTT‑TGCAGCCCGgtt                                                                                                                                  >  2:572268/1‑150 (MQ=255)

                  gAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGtttt                                                                                                                                 >  2:727481/1‑150 (MQ=255)

                   aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTtc                                                                                                                                >  2:3398251/1‑150 (MQ=255)

                    aTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTtct                                                                                                                               >  1:1595166/1‑150 (MQ=255)

                     tagcccgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACCCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTtctc                                                                                                                              <  2:3147779/144‑1 (MQ=255)

                     tAGACCGCGGCGGCCGCAGTTTTCCTGTCCGGCGTCACCCAGTACATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTtctc                                                                                                                              <  2:3604813/150‑1 (MQ=255)

                     tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTtctc                                                                                                                              <  2:1931217/150‑1 (MQ=255)

                     tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTCAGCAGCCCGTTTtctc                                                                                                                              >  2:3690030/1‑150 (MQ=255)

                      aGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACCTCCCTCCCCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCa                                                                                                                             >  1:868979/1‑150 (MQ=255)

                      aGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCAATAAAAAGACCATCCACACCTGTAAAGATACAAGCAAACGTTTCATAGAAAAGCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TCTTT‑AGCAGCCCGTCTTCTCa                                                                                                                             <  1:3411237/150‑1 (MQ=255)

                       gACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAg                                                                                                                            >  1:1784573/1‑150 (MQ=255)

                          cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCTAAAAGACCATCCCCACCCGTAAAGATACAAGCACACCTTTCATAGAAAAACATCCCTCCCCTTCTTTTTGGCTGGCAGC‑CTT‑TTT‑AGCACCCCGTTTTCTCTGCCg                                                                                                                         >  2:2601134/2‑150 (MQ=255)

                            cGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGC‑CTT‑TTT‑AGCTGCCCGTTTTCTCAGCCGCa                                                                                                                       >  1:2333669/1‑150 (MQ=255)

                             ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAg                                                                                                                      <  1:101247/150‑1 (MQ=255)

                                aGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGccc                                                                                                                   >  2:1700496/1‑150 (MQ=255)

                                 gCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGcccg                                                                                                                  >  1:1895126/1‑149 (MQ=255)

                                  ccGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAGAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGcccgc                                                                                                                 >  1:920438/1‑148 (MQ=255)

                                  ccGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGcccgc                                                                                                                 >  2:1273619/1‑148 (MQ=255)

                                      aGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGcccgcaaga                                                                                                             <  1:2581187/150‑7 (MQ=255)

                                         ttttCTGTCCGGTGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGaaaa                                                                                                          >  1:754257/1‑150 (MQ=255)

                                          tttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAg                                                                                                         >  2:600556/1‑150 (MQ=255)

                                            tCTGTCCGGCGCCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGAc                                                                                                       <  1:2879507/150‑1 (MQ=255)

                                             cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGGAAAGACg                                                                                                      >  1:2835900/1‑150 (MQ=255)

                                             cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACg                                                                                                      >  2:3784780/1‑150 (MQ=255)

                                              tGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACgg                                                                                                     <  1:2271838/150‑1 (MQ=255)

                                               gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCACGAAAAGACGGc                                                                                                    >  1:2316077/1‑150 (MQ=255)

                                               gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc                                                                                                    <  2:387429/150‑1 (MQ=255)

                                                tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGGCGGcc                                                                                                   >  1:1393094/1‑150 (MQ=255)

                                                       tCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttct                                                                                            <  2:1887246/150‑1 (MQ=255)

                                                        cACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGTTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttctt                                                                                           >  1:694069/1‑150 (MQ=255)

                                                             aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAGGAAAAGACCGCCGATTTCTtctctc                                                                                      >  1:3721312/1‑150 (MQ=255)

                                                                tcatcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCa                                                                                   >  1:3121831/1‑150 (MQ=255)

                                                                  atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCaaa                                                                                 <  1:638014/150‑1 (MQ=255)

                                                                   tcaATAAGACCACCCCCACCCGTAAAGATCCAAGCAAACGTTTCATAGAAAACCATCCCTCCCTTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc                                                                                <  2:2397502/150‑1 (MQ=255)

                                                                   tcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc                                                                                <  1:119735/150‑1 (MQ=255)

                                                                     aaaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGGCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTa                                                                              >  1:3934416/1‑150 (MQ=255)

                                                                       aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTGAGCAGCCCGTTTTCTCAGCCGCAGCCCGAACGAAAAGGCGGCCGATTTCTTTGCTCGCAAACTAGc                                                                            >  2:2148115/1‑150 (MQ=255)

                                                                           aCCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTCTTCTGGCAAACTAGCGGct                                                                        >  1:1922067/1‑150 (MQ=255)

                                                                                ccACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGc                                                                   >  2:2271302/1‑150 (MQ=255)

                                                                                 cacaCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                  >  2:549656/1‑150 (MQ=255)

                                                                                   cacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGGAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaa                                                                >  1:1140564/1‑150 (MQ=255)

                                                                                     cccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                              <  2:1847127/150‑1 (MQ=255)

                                                                                      ccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                                             >  1:3554264/1‑150 (MQ=255)

                                                                                       cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGGCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCCAGCAAAAccc                                                            >  2:2019492/1‑148 (MQ=255)

                                                                                       cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTc                                                            <  1:2081296/150‑1 (MQ=255)

                                                                                         tAAAGATCCAAGCACACGGAGCATAGCAAGACATCGCTGCGCTTCTCTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAGAAGACGGCCGATTTCTTTCCTCGCAAACTAGCGGCTCTAGCAAAACCCAt                                                          <  2:3339157/150‑1 (MQ=255)

                                                                                          aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCTGCCGCAGCCCGCTAGAAAAGACGGCCGATTTCTTTTCTCGCAACCCAGCGGCTCTAGCAAAACTCATg                                                         >  2:781346/1‑150 (MQ=255)

                                                                                          aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg                                                         >  2:269014/1‑150 (MQ=255)

                                                                                           aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                                        >  1:2960718/1‑150 (MQ=255)

                                                                                           aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCACAGCCGCAGCCCGCAAGAAAAGACGACCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                                        <  1:3340393/150‑1 (MQ=255)

                                                                                            aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAc                                                       >  2:1018885/1‑150 (MQ=255)

                                                                                               tACAAGCAAACGTTTCATAGAAAACCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActct                                                    <  1:2726393/150‑1 (MQ=255)

                                                                                               tACAAGCAAACGTTTCATAGAAAAACATCTCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCCCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGGAAAACTCATGActct                                                    <  1:3970513/150‑1 (MQ=255)

                                                                                               tACAAGCAAACGGTTCATAAAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCACCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGGTGCCTTGTCAGGCAAACTAGCGGCTCAAGCAAAACACATGAccct                                                    >  1:3629980/1‑150 (MQ=255)

                                                                                                aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTa                                                   >  1:2162826/1‑150 (MQ=255)

                                                                                                   aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa                                                >  2:1377865/1‑150 (MQ=255)

                                                                                                    gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGGCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCTCTAGCCAAACTCATGGCCCTTTGAt                                               >  1:646558/1‑150 (MQ=255)

                                                                                                      aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAg                                             <  2:3261746/150‑1 (MQ=255)

                                                                                                       atcttttCATAGAAAACCATCCCTCCGCTCCTTTTTGGCAGCCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCCCAGCCGCAGCCCGCAAGAAATGCCGCCCGATTTCTTTTCTCGCAATCTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGa                                            <  2:40570/146‑1 (MQ=255)

                                                                                                        aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc                                           <  1:186311/150‑1 (MQ=255)

                                                                                                          gTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCg                                         >  2:1119188/1‑150 (MQ=255)

                                                                                                           tttCATAGAAAACCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGt                                        <  2:2540889/150‑1 (MQ=255)

                                                                                                            ttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTg                                       <  2:2752996/150‑1 (MQ=255)

                                                                                                            ttCATAGAAAAACATACCTCCGCTTCTTTTTGGCGGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGTCGCATACCCCAAGAAAAGTCGGGCGATTTTTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGACTCTATGATAGACGGTg                                       >  1:2383293/1‑150 (MQ=255)

                                                                                                             tCATAGAAAAGCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCGGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg                                      <  1:3028896/150‑1 (MQ=255)

                                                                                                             tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg                                      >  1:493350/1‑150 (MQ=255)

                                                                                                               aTAGAAAACCATCCCTGCGCTCCTTTTTGGCAGGCAGCGT‑T‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                    <  1:1663876/150‑1 (MQ=255)

                                                                                                                 aGAAAAACATCCCTCCGCTCCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAag                                  <  1:2036829/150‑1 (MQ=255)

                                                                                                                   aaaaaCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAagag                                <  1:3284239/150‑1 (MQ=255)

                                                                                                                       aCATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAtt                            <  1:2466480/150‑1 (MQ=255)

                                                                                                                        cATCCCTCCGCCTCTTTTGGGCAGGCAGC‑CTT‑TTT‑AGCAGCCGGTCTTCACAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTc                           <  1:3995215/150‑1 (MQ=255)

                                                                                                                         aTCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                          <  2:3936619/150‑1 (MQ=255)

                                                                                                                          tCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTTTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTTTGATAGACCGTGGAAAGGGAATTCtt                         >  1:442979/1‑150 (MQ=255)

                                                                                                                          tCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt                         >  2:3460637/1‑150 (MQ=255)

                                                                                                                           cccTCCGCTTCTTTTTCGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTGTCTCAGCCGCAGCCCGCAAGAAACGACGCCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCttt                        <  1:3100322/150‑1 (MQ=255)

                                                                                                                            ccTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTg                       <  2:3520157/150‑1 (MQ=255)

                                                                                                                            ccTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTg                       <  1:981818/150‑1 (MQ=255)

                                                                                                                             cTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTgg                      >  1:3603321/1‑149 (MQ=255)

                                                                                                                               ccGCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTca                    <  2:1305344/150‑1 (MQ=255)

                                                                                                                               ccGCCTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCCCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACACATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTca                    <  2:285718/150‑1 (MQ=255)

                                                                                                                                 gCTTCTTTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTctca                  >  2:2960516/1‑150 (MQ=255)

                                                                                                                                     cttTTTGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCCAAACTCATGACTCTATGATAGACCGTGGAAAGAGGATTCTTTGTCACAatat              >  1:2670181/1‑150 (MQ=255)

                                                                                                                                         ttGGCAGGCAGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGt          >  1:2524409/1‑150 (MQ=255)

                                                                                                                                           ggcaggcaGC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGa        >  1:547220/1‑150 (MQ=255)

                                                                                                                                              aggcagC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCCCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGCCACAATATGAGTGaaaa     <  2:1521572/150‑1 (MQ=255)

                                                                                                                                                 cagC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAc  <  2:920438/150‑1 (MQ=255)

                                                                                                                                                 cagC‑CTT‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAAAGAATTCTTTGTCACAATATGAGGGaaaaaaa  >  1:2439486/1‑149 (MQ=255)

TGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTT‑AGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAC  >  CP006881/3725056‑3725349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`