Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,534 G→A L116L (TTG→TTA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,5340GA99.1% 1120.3 / NA 334L116L (TTG→TTA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (140/189);  total (140/194)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

TTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTG  >  CP006881/3723388‑3723680
                                                                                                                                                  |                                                                                                                                                  
ttCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATc                                                                                                                                                 <  1:643996/150‑1 (MQ=255)
 tCGGGGCCGCTTTTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCg                                                                                                                                                <  2:3614758/150‑1 (MQ=255)
    gggCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTCCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGgcg                                                                                                                                             <  2:2601882/150‑1 (MQ=255)
      gCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTGCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGcc                                                                                                                                           <  1:251785/150‑1 (MQ=255)
      gCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCCCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGGCAAGCTATGCCGGCAATTTAATCGGCGcc                                                                                                                                           >  2:478642/1‑150 (MQ=255)
       ccGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGGGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                                                                                                          <  1:1804426/150‑1 (MQ=255)
       ccGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                                                                                                          <  1:1549964/150‑1 (MQ=255)
        cGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAt                                                                                                                                         >  1:3932343/1‑150 (MQ=255)
              tCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACCCCCTATACTTGTGGATGTCAAGCTAGGCCGGCAATTTAATCGGCGCCATTCTGtt                                                                                                                                   >  2:112679/1‑150 (MQ=255)
                tgatgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCCCCGGAAAAAAATCAGCTGGCGGGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                                                                                 <  1:3472034/147‑1 (MQ=255)
                 gatgATTGCTTACGGAGGCGGGGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAAATTAATCGGCGCCATTCTGTTTGc                                                                                                                                >  1:1283893/3‑150 (MQ=255)
                  atgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGcc                                                                                                                               >  2:181239/2‑150 (MQ=255)
                       tGCTTACGGAGGCGGAGATTTATTTCCCGGCAACACCTTTTATTTCCCCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATGCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt                                                                                                                          <  1:2656789/150‑1 (MQ=255)
                        gCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTg                                                                                                                         <  1:3437904/150‑1 (MQ=255)
                         cTTACGGAGGCTGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTgg                                                                                                                        >  2:1018013/1‑149 (MQ=255)
                         cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGa                                                                                                                        <  1:1115312/150‑1 (MQ=255)
                           tACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATc                                                                                                                      <  1:560431/150‑1 (MQ=255)
                           tACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATc                                                                                                                      <  1:2434234/150‑1 (MQ=255)
                           tACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGGTTGCCATCCTGATc                                                                                                                      >  2:3527897/1‑150 (MQ=255)
                            aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCa                                                                                                                     <  1:3098247/150‑1 (MQ=255)
                            aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCa                                                                                                                     <  2:3572425/150‑1 (MQ=255)
                             cGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAg                                                                                                                    <  1:1856654/150‑1 (MQ=255)
                               gAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcg                                                                                                                  <  1:1725658/150‑1 (MQ=255)
                               gAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCCCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcg                                                                                                                  <  1:1204432/150‑1 (MQ=255)
                                aGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCTgcgc                                                                                                                 >  2:1565466/1‑150 (MQ=255)
                                aGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcgc                                                                                                                 <  1:940430/150‑1 (MQ=255)
                                aGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcgc                                                                                                                 <  1:1637488/150‑1 (MQ=255)
                                  gCGGAGATTTATTTACCGGCACCACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGa                                                                                                               <  1:2856639/150‑1 (MQ=255)
                                  gCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGATTGCCATCCTGATCAGCGCGa                                                                                                               >  2:2543406/1‑150 (MQ=255)
                                    ggAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAcg                                                                                                             >  2:888556/1‑149 (MQ=255)
                                    ggAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAcg                                                                                                             <  1:432852/150‑2 (MQ=255)
                                         tttatttaCCGGCACCACCTTTTTTTTCACCTATACCGCGCACCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACt                                                                                                        <  2:3849849/150‑1 (MQ=255)
                                           tatttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAGAATCAGCTGGCGCGACACCCTACACTTGTGGATGTCAAGCTATGCCGGCAATTTAGTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGGCGGGGCttt                                                                                                      >  2:3685587/1‑150 (MQ=255)
                                            atttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACtttt                                                                                                     <  1:2729921/150‑1 (MQ=255)
                                             tttaCCGGCAACACCTTTTATTTCAGCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                                                                                                    <  1:3379852/150‑1 (MQ=255)
                                             tttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                                                                                                    <  2:2247239/150‑1 (MQ=255)
                                              ttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTg                                                                                                   <  1:1655668/150‑1 (MQ=255)
                                                accggCAACTCCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                 <  2:3382242/147‑1 (MQ=255)
                                                accggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                 >  1:2026628/4‑150 (MQ=255)
                                                 ccggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagg                                                                                                >  2:3173767/3‑150 (MQ=255)
                                                  cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                               >  2:251048/2‑150 (MQ=255)
                                                   ggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACGCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgaggag                                                                                              <  1:194998/150‑1 (MQ=255)
                                                   ggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgaggag                                                                                              >  1:2358896/1‑150 (MQ=255)
                                                      aaCACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCt                                                                                           <  2:3104567/150‑1 (MQ=255)
                                                      aaCACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCt                                                                                           <  1:516511/150‑1 (MQ=255)
                                                       acacCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                          <  1:1422858/150‑1 (MQ=255)
                                                        cacCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTc                                                                                         <  1:206855/150‑1 (MQ=255)
                                                        cacCTTTTATTTCACCTATACCCCGCTCCGGAAACAAATCAGCTGGCGCGACACCCTATCCTTGTGGATGTCAAGCTATGCCGGCAATCTACCCGGCGCCATTCTGTTTGCCGTCCTGATCAGCGCGACGGGACTTTCTGTGGTGCCTTc                                                                                         >  2:728902/1‑150 (MQ=255)
                                                          ccttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGTGGAGCCTTCTg                                                                                       >  2:3307062/3‑150 (MQ=255)
                                                              ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGGTCa                                                                                   >  2:1767549/1‑150 (MQ=255)
                                                                   tCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGGCGGGGCTTTTTGAGGAGCCTTCTGTTCATTCCt                                                                              >  2:1298859/1‑150 (MQ=255)
                                                                    cACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                             >  2:77524/1‑150 (MQ=255)
                                                                       ctataCCGGGCCCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCttttt                                                                          <  1:2174048/149‑1 (MQ=255)
                                                                       ctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGGGCCTTCTGTTCATTCCttttt                                                                          >  2:2160049/2‑150 (MQ=255)
                                                                          taCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGGTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAt                                                                       >  1:3967970/1‑150 (MQ=255)
                                                                            ccGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTc                                                                     <  1:1505081/150‑1 (MQ=255)
                                                                                  tCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTCTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGGTTCATCTgg                                                               >  2:2670108/1‑150 (MQ=255)
                                                                                   ccGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGGCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTCTGGTTCATTTGGc                                                              >  2:2384713/1‑150 (MQ=255)
                                                                                      gaaaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTGTGATTCATTTGGCagg                                                           >  2:2412255/2‑149 (MQ=255)
                                                                                       aaaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCagag                                                          >  2:398114/1‑150 (MQ=255)
                                                                                        aaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGc                                                         <  1:270252/150‑1 (MQ=255)
                                                                                        aaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGc                                                         >  2:1368043/1‑150 (MQ=255)
                                                                                           aaatcagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGcaca                                                      <  1:3527897/144‑1 (MQ=255)
                                                                                             atcagCTGGGGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAGCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACaaa                                                    <  1:358067/146‑1 (MQ=255)
                                                                                              tcagCTGGCGCGACACCCTATACTGGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACaaaa                                                   <  1:1380435/147‑1 (MQ=255)
                                                                                               caggtGGCGCGACACCCTATATTTGTGGAAGTCAAGTTGTGCCGGCAATTTAATCGGCCCCATTTTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAt                                                  <  1:3239816/146‑1 (MQ=255)
                                                                                               cagCTGGCGCGACACCCTATCCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTAGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAt                                                  <  1:3173767/148‑1 (MQ=255)
                                                                                                  cTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGGTTCATTTGGCAGAGCACAAAATGGa                                               >  1:3185004/1‑150 (MQ=255)
                                                                                                  cTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAACCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGa                                               <  2:686117/150‑1 (MQ=255)
                                                                                                   tGGCGCGACACCCTTTACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAg                                              <  1:3835396/150‑1 (MQ=255)
                                                                                                   tGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTGTTGATTCATTTGGCAGAGCACAAAATGGAg                                              >  1:3358834/1‑150 (MQ=255)
                                                                                                   tGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCAGATCAGCGGGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAGAATGGAg                                              <  1:279827/150‑1 (MQ=255)
                                                                                                   aggCGCGACACCCTATTCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGaa                                              <  1:3713441/149‑2 (MQ=255)
                                                                                                    ggCGCGACCCCCTTTACTTGTGGATGACAAGCTATGCCGGCAATTTAACCGGCCCCCTTCTGTTTGCCACCCTGACCAGCGCGGCGGGACTTTTTGAGGAGCCTTCTGTTCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgc                                             <  1:2301398/150‑1 (MQ=255)
                                                                                                        cgaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAGTCGGCGCCATTCTGTTTGCCATCCTGGTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTGTGATTCATTTGGCAGAGCACAAAATGGAgccgcc                                         >  2:1992058/2‑150 (MQ=255)
                                                                                                         gaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccg                                        >  2:1893656/1‑149 (MQ=255)
                                                                                                             cccTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGGGCACAAAATGGAGCCGCCGGCtt                                    >  1:2490384/1‑150 (MQ=255)
                                                                                                             cccTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                    >  2:2313868/1‑150 (MQ=255)
                                                                                                              ccTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTc                                   >  2:573908/1‑150 (MQ=255)
                                                                                                                  tccttgtgGATGCCAAGCTAGGCCGGCAATTTAATCGGCCCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaa                               <  1:2769334/146‑1 (MQ=255)
                                                                                                                  tACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGTTCAGCGCGACGGGACTCTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGTGCACAACATGGAGCCGCCGGCTTCCGaa                               >  1:3307035/1‑150 (MQ=255)
                                                                                                                     tgggggATGCCAACCTGTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                            <  1:1064499/146‑1 (MQ=255)
                                                                                                                      tgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGt                           >  1:3064618/1‑150 (MQ=255)
                                                                                                                      tgtgGATGCCAAGCTATGCCGGCAATTTATCCGGCGCCATTCTGTTTGCCATCCTGATCAGGGCGAGGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGt                           <  1:2139855/150‑1 (MQ=255)
                                                                                                                       gtgGATGCCAAGCTATGCCGGCAATTTAACCGGCGCCTTTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGtt                          <  1:1003567/150‑1 (MQ=255)
                                                                                                                             ggcAAGCTAGGCCGGCAATTTATCCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCag                    <  2:2703017/148‑1 (MQ=255)
                                                                                                                              tCAAGCTCTGCCGGCATTTTACTCGGCGCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGTTTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCaga                   <  1:3656747/150‑1 (MQ=255)
                                                                                                                               cAAGCTATGCCGGAATTTTAACCGGCGCCATTCTGTTTGCCACCCTGATCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCCCAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag                  <  2:3142825/150‑1 (MQ=255)
                                                                                                                                  gcaacgCCGGCAATTTAATCGGCGGCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCTTTTTTGATTCATTTGGCAGAGCACAAAATGGACCCGCCGGCTTCCGAATTGTTTTTCAGAggaa               <  1:781219/145‑3 (MQ=255)
                                                                                                                                  gCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAggaa               >  2:1446148/1‑148 (MQ=255)
                                                                                                                                   cTATGCCGCAAATTTAATCGGCGCCTTTCTGTTTGCCATCCTGATCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAAt              <  2:2970086/150‑1 (MQ=255)
                                                                                                                                    tgtGCCGGCATTTTCATCGGCGCTCTTCTGTTTGCCATCCTGACCAGCGGGACGGGACTTTTTGAGGAGCCTTCTGTACATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg             <  1:895718/148‑1 (MQ=255)
                                                                                                                                     aggCCGGCAATTTAACCGGCGCCATTCTGTTTGCCACCCTGACCAGCGCGCCGGGACTTTTTGGGGCGCCTCCTGTCCCTTCCTTTTTGATTCATTGGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGc            <  2:3209138/148‑1 (MQ=255)
                                                                                                                                     aTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGGTTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGc            >  2:2450118/1‑150 (MQ=255)
                                                                                                                                      tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCACCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCt           <  1:3015789/150‑1 (MQ=255)
                                                                                                                                       gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtg          >  2:3484635/1‑150 (MQ=255)
                                                                                                                                          ggCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGc       >  2:2338862/1‑150 (MQ=255)
                                                                                                                                             aaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGATTTTCAGAGGGATGCTGTGCAAt    >  2:635140/1‑150 (MQ=255)
                                                                                                                                               tttAATCGGCGCCTTTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCTTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTg  <  2:1047205/150‑1 (MQ=255)
                                                                                                                                               tttAATCGGCGCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTg  <  1:838586/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTG  >  CP006881/3723388‑3723680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑