Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,616 C→T L144L (CTG→TTG)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,6160CT99.7% 1111.4 / NA 315L144L (CTG→TTG) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (135/178);  total (136/179)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 315 total aligned reads displayed.

CTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTC  >  CP006881/3723469‑3723762
                                                                                                                                                   |                                                                                                                                                  
    ggaaaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCag                                                                                                                                              <  1:1110097/148‑1 (MQ=255)
       aaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGc                                                                                                                                           <  1:270252/150‑1 (MQ=255)
       aaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGAAGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGc                                                                                                                                           <  1:2027302/150‑1 (MQ=255)
          aaatcggCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATGGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGcaca                                                                                                                                        <  1:623018/144‑1 (MQ=255)
              caggtGGCGCGACACCCTATATTTGTGGAAGTCAAGTTGTGCCGGCAATTTAATCGGCCCCATTTTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAt                                                                                                                                    <  1:3239816/146‑1 (MQ=255)
              cagCTGGCGCGACACCCTATCCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTAGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAt                                                                                                                                    <  1:3173767/148‑1 (MQ=255)
              cagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGGGCACAAAAt                                                                                                                                    >  2:698759/3‑150 (MQ=255)
               agCTGGCGCGACACCCTATACTTGTGGATGCCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATg                                                                                                                                   <  1:1732716/149‑1 (MQ=255)
                gCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATgg                                                                                                                                  <  2:342730/150‑1 (MQ=255)
                 cTGGCGCGCCACCCTATCCTTGTGGATGCCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGa                                                                                                                                 <  1:3545169/150‑1 (MQ=255)
                  tGGCGCGACACCCTTTACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAg                                                                                                                                <  1:3835396/150‑1 (MQ=255)
                  tGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCCCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAg                                                                                                                                >  1:3358836/1‑150 (MQ=255)
                   ggCGCGACCCCCTTTACTTGTGGATGACAAGCTATGCCGGCAATTTAACCGGCCCCCTTCTGTTTGCCACCCTGACCAGCGCGGCGGGACTTTTTGAGGAGCCTTCTGTTCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgc                                                                                                                               <  1:2301398/150‑1 (MQ=255)
                        gaCACCCTTTACTTGTGGATGTCAAGCTGTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGCCGGGACTTTTTGGGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccg                                                                                                                          <  2:2545885/150‑2 (MQ=255)
                        gaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccg                                                                                                                          >  2:2408472/1‑149 (MQ=255)
                        gaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccg                                                                                                                          >  2:2511599/1‑149 (MQ=255)
                            cccTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                                                                                      >  2:478637/1‑150 (MQ=255)
                               tatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                                                                                                   >  2:2635682/3‑150 (MQ=255)
                                 tccttgtgGATGCCAAGCTAGGCCGGCAATTTAATCGGCCCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaa                                                                                                                 <  1:2769334/146‑1 (MQ=255)
                                 tACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCgga                                                                                                                 >  2:1772380/1‑148 (MQ=255)
                                  acttgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGGGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaat                                                                                                                >  1:3350678/4‑149 (MQ=255)
                                     tgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGt                                                                                                             >  1:3064618/1‑150 (MQ=255)
                                     tgtgGATGCCAAGCTATGCCGGCAATTTATCCGGCGCCATTCTGTTTGCCATCCTGATCAGGGCGAGGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGt                                                                                                             <  1:2139855/150‑1 (MQ=255)
                                      gtgGATGCCAAGCTATGCCGGCAATTTAACCGGCGCCTTTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGtt                                                                                                            <  1:1003567/150‑1 (MQ=255)
                                       tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttt                                                                                                           >  1:1921406/1‑150 (MQ=255)
                                            gccAAGCTATGCCGGCATTTTAATCGGCGCCATTCTGTTTGCCACCCTGATCAGCGCGACGGGACTTTTGGAGGAGCCTCCAGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCag                                                                                                      <  1:3260497/148‑1 (MQ=255)
                                             tCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGGGCACAAAATGGAGCCGCCGGCTTCCGAAGTGTTTTTCagg                                                                                                     >  1:2553415/1‑149 (MQ=255)
                                              cAAGCTATGCCGGAATTTTAACCGGCGCCATTCTGTTTGCCACCCTGATCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCCCAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCagag                                                                                                    <  2:3142825/150‑1 (MQ=255)
                                                 gcaacgCCGGCAATTTAATCGGCGGCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCTTTTTTGATTCATTTGGCAGAGCACAAAATGGACCCGCCGGCTTCCGAATTGTTTTTCAGAggaa                                                                                                 <  1:781219/145‑3 (MQ=255)
                                                 gCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAggaa                                                                                                 >  2:1446148/1‑148 (MQ=255)
                                                  cTATGCCGCAAATTTAATCGGCGCCTTTCTGTTTGCCATCCTGATCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAAt                                                                                                <  2:2970086/150‑1 (MQ=255)
                                                   tgtGCCGGCATTTTCATCGGCGCTCTTCTGTTTGCCATCCTGACCAGCGGGACGGGACTTTTTGAGGAGCCTTCTGTACATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                                                                               <  1:895718/148‑1 (MQ=255)
                                                    aTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGGTTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGc                                                                                              >  2:2450118/1‑150 (MQ=255)
                                                       ccGGCAATTTATTCGGCGCCTTTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtgt                                                                                           <  1:1059680/150‑1 (MQ=255)
                                                       ccGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTGTTCAGAGGAATGCtgt                                                                                           >  2:1404303/1‑150 (MQ=255)
                                                         ggCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGc                                                                                         <  2:598418/150‑1 (MQ=255)
                                                         ggCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGc                                                                                         >  2:2338862/1‑150 (MQ=255)
                                                          gCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAACATGGAGCCGCCGGCTTCCGAATTGTTTGTCAGAGGAATGCTGGGCa                                                                                        >  2:3218351/1‑150 (MQ=255)
                                                            aaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAt                                                                                      >  2:2678485/1‑150 (MQ=255)
                                                             ttgaaagcGGGGCCTTTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGGGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCGGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                                                                                     <  1:2412255/143‑1 (MQ=255)
                                                             aTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                                                                                     <  1:2160049/150‑1 (MQ=255)
                                                              tttaaccGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTg                                                                                    <  2:821307/144‑1 (MQ=255)
                                                              tttAATCGGCGCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTg                                                                                    <  1:838586/150‑1 (MQ=255)
                                                                taagcGGCGCCATTCTGTTTGCCATCCGGATCAGGGCGACGGGACTTTTTGAGGAGCCTTCTGTCCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCGGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAATGGc                                                                                  <  1:2380790/146‑1 (MQ=255)
                                                                 aatgggCGCCATCCTGCTTCCCATCCTGACCAGCGCGCCGGGACTTTTTGAGGACCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCt                                                                                 <  1:2334500/146‑1 (MQ=255)
                                                                 aaTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCt                                                                                 >  2:1936595/2‑150 (MQ=255)
                                                                    gggCCCCATTCTGTTTGCCACCCGGACCAGGGCGACGGGATTTTTTGAGGAGCCTTCTGTTCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgt                                                                              <  1:2603382/149‑1 (MQ=255)
                                                                     ggCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtg                                                                             >  2:3008975/1‑150 (MQ=255)
                                                                      gcgcCTTTCTGTTGGCCATCCTGATCAGCGCGCGGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTCGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt                                                                            <  1:3632294/150‑1 (MQ=255)
                                                                      gcgcCATTCTGTTTGCCCTCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTCCATTCCTTTTTGCTTCATTTGGCAGGGCACAAAATGGAGCCGCCGGCCTCCGGATTGTTTTTCACCGGAATGCTGTGCAATTGGCTtgtgg                                                                            >  1:1416070/1‑149 (MQ=255)
                                                                      gcgcCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt                                                                            <  2:3117414/150‑1 (MQ=255)
                                                                       cgcCATTCTTTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgtg                                                                           <  1:1712719/150‑1 (MQ=255)
                                                                       cgcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgtg                                                                           <  1:1461798/150‑1 (MQ=255)
                                                                       cgcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgtg                                                                           >  2:2250651/1‑150 (MQ=255)
                                                                          ctttCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTGTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCt                                                                        <  1:2449608/148‑1 (MQ=255)
                                                                           aTTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTGTTTCAGAGGGATGCTGGGCAATTGGCTTGTGGGGCTc                                                                       >  2:417497/1‑150 (MQ=255)
                                                                            ttCTGTCTGCCATCCTGCTCAGCGCGCCGGGACTTTTTGAGGAGCCTTCTGTTCATTTCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCg                                                                      <  2:2026628/150‑1 (MQ=255)
                                                                              ttgtttGCCATCCTGACCAGCGCGACGGGTCTTTTTGAGGACCCTTCTGTTCATTCTTTTTTGATTCATTTGGCAGAGCACAAAATGGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGcc                                                                    <  1:3506718/147‑1 (MQ=255)
                                                                                gtttGCCATCCTGATCAGCGCGACGGGCCTTTTTGAGGAGCCTTCTGTCCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtt                                                                  <  2:537859/149‑1 (MQ=255)
                                                                                 tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGGTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGGGCCTCGCCttt                                                                 >  2:2303217/1‑150 (MQ=255)
                                                                                  ttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCTCAAAATGGAGCCCCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGGGCAATTGGCTTGGGTGCCTCGCCtttg                                                                >  2:1172538/1‑149 (MQ=255)
                                                                                     ccATCCTGAGCAGCGGGACGGGCCTTGTTGAGGAGCCTTCTGTCCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCa                                                             <  1:1285853/150‑1 (MQ=255)
                                                                                      cctccTGATCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAt                                                            <  1:3670014/146‑1 (MQ=255)
                                                                                       atccTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                           >  2:1261067/4‑150 (MQ=255)
                                                                                       atccTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGGTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt                                                           >  2:1893444/4‑150 (MQ=255)
                                                                                            gctCAGCGCGACGGGTCTTTTTGAGGAGCCTTCTGTTCATTCTTTTTTGCTTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAt                                                      <  2:3932343/148‑1 (MQ=255)
                                                                                                aGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGtct                                                  <  1:3426451/150‑1 (MQ=255)
                                                                                                 cggcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGATCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGtctc                                                 <  1:1767549/148‑1 (MQ=255)
                                                                                                  cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTctct                                                >  2:2126140/1‑150 (MQ=255)
                                                                                                     gACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGGGCACAAAAGGGAGCCGCCGGCTTCCGAATTCTCGTTCAGAGGGATGCTGGGCAATCGGCTTGAGTGCCTCGCCATTTTCCTTCCAATGTCTCTCaa                                             >  2:500417/1‑150 (MQ=255)
                                                                                                       cgggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaag                                           >  2:3657700/6‑150 (MQ=255)
                                                                                                          gccTTTTGGAGGAGCCTTCTGTCCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggg                                        <  2:3537609/148‑1 (MQ=255)
                                                                                                          gacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATCGGCTTGGGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggg                                        >  1:2809008/3‑150 (MQ=255)
                                                                                                          gacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAAGGAGCCGCCGGCTTCCGGATTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggg                                        >  1:3684764/3‑150 (MQ=255)
                                                                                                                   aggagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTCTTCATTCCAATGTCTCTCAAAGGGGAGGGGGCaa                               >  1:1626738/1‑150 (MQ=255)
                                                                                                                   aggacccTTCTGTTCTTTCTTTTTTGATTCATTTGGCAGAGCACAAAGTGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCaa                               <  1:1160315/144‑1 (MQ=255)
                                                                                                                    ggagcCTTCTGTCCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAGTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCaaa                              <  1:710460/150‑1 (MQ=255)
                                                                                                                        ccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGGGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCGTTTTTCATTCCAAAGGCTCTCAAAGGGGAAGGAGCAAGGctt                          >  2:2890063/2‑149 (MQ=255)
                                                                                                                        ccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATGGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAATGGGGAAGGAGCAAAGctt                          >  2:3581782/2‑149 (MQ=255)
                                                                                                                         cTTCGGTCCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                         <  1:393725/150‑3 (MQ=255)
                                                                                                                          ttCTTTCCATTCTTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                        <  1:974693/150‑1 (MQ=255)
                                                                                                                          ttCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGGGCAGAGctttg                        >  2:1528631/1‑147 (MQ=255)
                                                                                                                          ttCTGTCCTTTCCTTTTTGATTCTTTTGGGAGGCCACAAATGGGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                        <  1:74462/150‑1 (MQ=255)
                                                                                                                           tCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTtttt                       <  1:1368043/150‑1 (MQ=255)
                                                                                                                            cTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTa                      >  1:548151/1‑150 (MQ=255)
                                                                                                                               ttCATTCCTTTTTGATTCATTGGGCAGAGAACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCa                   <  1:3307062/150‑1 (MQ=255)
                                                                                                                                tCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCat                  <  1:2499676/150‑1 (MQ=255)
                                                                                                                                 cATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatg                 >  2:1046279/1‑150 (MQ=255)
                                                                                                                                 cATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatg                 <  1:3153130/150‑1 (MQ=255)
                                                                                                                                  aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatga                <  1:1083741/150‑1 (MQ=255)
                                                                                                                                   ttCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatgat               >  1:3746134/1‑150 (MQ=255)
                                                                                                                                     tttttttGATTCATTTGGGAGAGCACAAAATGAAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGc             <  1:2197847/148‑1 (MQ=255)
                                                                                                                                     ccTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGc             <  2:3967970/150‑1 (MQ=255)
                                                                                                                                      cTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCt            >  2:1716657/1‑150 (MQ=255)
                                                                                                                                      cTTTTGGATTCATTTGGAAGAGCACAAAATGAACCCGCGGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCt            <  2:3185004/150‑1 (MQ=255)
                                                                                                                                       tttttGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAAAGCTGTGCAAATGGCTTGTGTGCCACGCCTTTTTCATTCCCATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGAtt           >  1:2882183/1‑150 (MQ=255)
                                                                                                                                         tttGATCCATTTGGCAGAGCCCAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCtttt         <  2:3307035/150‑1 (MQ=255)
                                                                                                                                            gATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGt      >  1:458676/1‑150 (MQ=255)
                                                                                                                                            gATTCATTTGGCAGAGCACAAAATGGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGt      <  1:1808952/150‑1 (MQ=255)
                                                                                                                                              ttCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGttt    >  2:3745768/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTC  >  CP006881/3723469‑3723762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑