Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,208 T→G R207R (AGA→CGA)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,2080TG99.7% 1117.2 / NA 289R207R (AGA→CGA) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (120/168);  minor base C (0/1);  total (120/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

AATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTAT  >  CP006881/3724061‑3724354
                                                                                                                                                   |                                                                                                                                                  
 aTGTTATTGATATTCATTTTTCAAAACCCCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCaa                                                                                                                                                 <  1:2166965/150‑1 (MQ=255)
     aaTTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGc                                                                                                                                             >  2:3523199/1‑150 (MQ=255)
       ttGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGt                                                                                                                                           <  2:2788618/150‑1 (MQ=255)
          atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAGGCATCTCCTCGTCCATACGATGGGGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGGGAc                                                                                                                                        >  2:968423/1‑150 (MQ=255)
          atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                                        >  2:441948/1‑150 (MQ=255)
          atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                                        >  1:2012283/1‑150 (MQ=255)
          atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                                        <  2:1551501/150‑1 (MQ=255)
          atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                                                        >  2:1855421/1‑150 (MQ=255)
           tatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCCTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa                                                                                                                                       >  1:1893119/1‑150 (MQ=255)
               catttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGc                                                                                                                                   >  2:3834052/4‑150 (MQ=255)
                atttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCg                                                                                                                                  >  1:746002/3‑150 (MQ=255)
                atttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCCTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGGCCAGGCg                                                                                                                                  >  2:1324898/3‑150 (MQ=255)
                  ttttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTAGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGtt                                                                                                                                <  2:2427812/150‑1 (MQ=255)
                    ttCAAAAACTCCGCCAGCTCCAGCGCACATTGGTAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGGGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGa                                                                                                                              >  2:662777/1‑150 (MQ=255)
                     tCAAAACCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCACCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGCCGTTGAt                                                                                                                             <  1:964090/150‑1 (MQ=255)
                     tCAAAAACTCCGCCAGCTCCAGGGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAt                                                                                                                             <  1:3492913/150‑1 (MQ=255)
                      cAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAtt                                                                                                                            >  2:3921863/1‑149 (MQ=255)
                        aaaaCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAgg                                                                                                                          <  2:2567057/150‑1 (MQ=255)
                          aaCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATCCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGaa                                                                                                                        <  1:3968640/150‑1 (MQ=255)
                          aaCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGGCAAGGCGTTGATAGGaa                                                                                                                        >  2:2351254/1‑150 (MQ=255)
                            ctccGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCCCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAAt                                                                                                                      <  1:2121305/150‑1 (MQ=255)
                             tccgCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATg                                                                                                                     <  2:3800334/147‑1 (MQ=255)
                             cccgCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATg                                                                                                                     <  2:1239962/147‑1 (MQ=255)
                                gCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTGGGTAGGAAATGCTg                                                                                                                  >  2:2468565/1‑150 (MQ=255)
                                 ccAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGt                                                                                                                 <  2:78422/150‑1 (MQ=255)
                                  cAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAAAAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGtt                                                                                                                <  1:1098793/150‑1 (MQ=255)
                                    gCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGtttt                                                                                                              <  2:413975/150‑1 (MQ=255)
                                    gCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGtttt                                                                                                              <  1:3403444/150‑1 (MQ=255)
                                          gcgcACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCa                                                                                                        >  2:2001263/1‑150 (MQ=255)
                                           cgcACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCAc                                                                                                       >  2:917278/1‑150 (MQ=255)
                                           cgcACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCAc                                                                                                       <  1:2953493/150‑1 (MQ=255)
                                              acaTTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCtt                                                                                                    >  1:1009595/1‑150 (MQ=255)
                                                aTTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTc                                                                                                  <  1:1799973/150‑1 (MQ=255)
                                                 ttGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCg                                                                                                 <  1:3740026/150‑1 (MQ=255)
                                                     tAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTa                                                                                             <  2:3490382/150‑1 (MQ=255)
                                                     tAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTa                                                                                             <  2:1367938/150‑1 (MQ=255)
                                                        gcgaaTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAg                                                                                          <  2:688390/147‑1 (MQ=255)
                                                           aaTGAATACTTTTTTTTGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTAt                                                                                       <  1:1478325/150‑1 (MQ=255)
                                                             tGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTg                                                                                     >  2:1198380/1‑150 (MQ=255)
                                                              gAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGt                                                                                    <  2:828996/150‑1 (MQ=255)
                                                               aaTACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGtt                                                                                   >  2:1117960/1‑150 (MQ=255)
                                                                aTACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGttt                                                                                  <  2:2130941/150‑2 (MQ=255)
                                                                 tACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGtttt                                                                                 <  1:3858567/150‑3 (MQ=255)
                                                                  aCTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTa                                                                                <  2:3118927/150‑1 (MQ=255)
                                                                  aCTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGACAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTa                                                                                <  1:116563/150‑1 (MQ=255)
                                                                   ttttttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCCTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGGGACAAGGCGTGGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAt                                                                               >  2:1724898/2‑150 (MQ=255)
                                                                    tttttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtt                                                                              >  1:2600049/1‑150 (MQ=255)
                                                                     ttttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAttt                                                                             >  1:1979544/1‑150 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAGTGCGTGACAAGGCGTTGATAGGAAATGCTGGTTTCATCCACCTTTCGTTTAAAGTATTGTTTTAtttt                                                                            >  2:3888340/1‑150 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                            >  2:263849/1‑150 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                            <  1:2544303/150‑1 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                            <  2:3658272/150‑1 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                            >  1:3235220/1‑150 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGGTTTAtttt                                                                            >  2:2878277/1‑150 (MQ=255)
                                                                        tttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTtct                                                                          <  2:1510924/150‑1 (MQ=255)
                                                                         ttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTtctc                                                                         <  1:580280/150‑1 (MQ=255)
                                                                          tGGATGAAATAAAGCATCTCCTCGTCCATCCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTctct                                                                        <  1:379985/150‑1 (MQ=255)
                                                                          tGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATCCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTctct                                                                        <  2:2289396/150‑1 (MQ=255)
                                                                            gATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTAt                                                                      >  2:1665999/1‑150 (MQ=255)
                                                                            gATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTAt                                                                      >  2:1482359/1‑150 (MQ=255)
                                                                              tGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTAACa                                                                    <  1:3300130/150‑1 (MQ=255)
                                                                               gAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAt                                                                   >  2:573479/1‑150 (MQ=255)
                                                                                aaaTAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                  <  1:1270643/150‑1 (MQ=255)
                                                                                   tAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                               <  2:3198337/150‑1 (MQ=255)
                                                                                      aGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTg                                                            <  2:2075719/150‑1 (MQ=255)
                                                                                       gCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGa                                                           <  1:1041119/150‑1 (MQ=255)
                                                                                        cATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGAt                                                          >  2:1886023/1‑150 (MQ=255)
                                                                                         aTCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATc                                                         <  1:868498/150‑1 (MQ=255)
                                                                                           ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                                       <  1:3152742/150‑1 (MQ=255)
                                                                                            tcctcGTCCATCCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATg                                                      <  1:2360826/150‑1 (MQ=255)
                                                                                             cctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                                     >  2:1052433/1‑150 (MQ=255)
                                                                                                cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGTTCATGGTCg                                                  >  2:3680334/1‑150 (MQ=255)
                                                                                                cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                  <  2:373314/150‑1 (MQ=255)
                                                                                                cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                  <  1:2073419/150‑1 (MQ=255)
                                                                                                  tCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                <  1:2732928/150‑1 (MQ=255)
                                                                                                      tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCt                                            <  2:3612865/150‑1 (MQ=255)
                                                                                                       aCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGCTAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                           <  1:2506199/150‑1 (MQ=255)
                                                                                                       aCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                           <  1:2255163/150‑1 (MQ=255)
                                                                                                       aCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                           <  1:1673655/150‑1 (MQ=255)
                                                                                                          aTGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAg                                        <  1:2435686/150‑1 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgc                                       >  2:461668/1‑150 (MQ=255)
                                                                                                                 gcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa                                 >  2:3042557/1‑150 (MQ=255)
                                                                                                                 gcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa                                 <  1:363169/150‑1 (MQ=255)
                                                                                                                  cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAAt                                <  1:14898/150‑1 (MQ=255)
                                                                                                                  cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAAt                                >  2:284656/1‑150 (MQ=255)
                                                                                                                  cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAAt                                >  2:1271124/1‑150 (MQ=255)
                                                                                                                    tCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATAc                              <  1:3042557/150‑1 (MQ=255)
                                                                                                                       tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg                           <  1:281109/150‑1 (MQ=255)
                                                                                                                         tGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGct                         >  2:2744779/1‑150 (MQ=255)
                                                                                                                           aTTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGctct                       >  2:740599/1‑150 (MQ=255)
                                                                                                                           aTTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGctct                       >  2:1761652/1‑150 (MQ=255)
                                                                                                                              ccAAGCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTACAGCGCTGAATACATGCTCTCCg                    <  1:2901757/150‑1 (MQ=255)
                                                                                                                                   cggctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatcc               <  2:2169489/147‑1 (MQ=255)
                                                                                                                                      ctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccatc            <  1:248509/150‑1 (MQ=255)
                                                                                                                                      ctgaccgCGTACCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTATCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccatc            <  1:1310956/150‑1 (MQ=255)
                                                                                                                                       tgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCt           <  1:1248996/150‑1 (MQ=255)
                                                                                                                                         accgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTc         <  1:3771079/147‑1 (MQ=255)
                                                                                                                                           cgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGc       <  1:441948/149‑1 (MQ=255)
                                                                                                                                              gTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGt    >  1:1617798/1‑150 (MQ=255)
                                                                                                                                               tATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTa   >  2:3986531/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTAT  >  CP006881/3724061‑3724354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑