Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,206 C→T 7.0% intergenic (–/‑772) – / → eryR –//vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,2060CT 7.0% 53950.4 / inf 15771intergenic (–/‑772)–/eryR–//vntifkey=4
Reads supporting (aligned to +/- strand):  ref base C (7710/6848);  new base T (565/585);  total (8294/7477)
Fisher's exact test for biased strand distribution p-value = 1.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-21

Only 100 of 16216 total aligned reads displayed.

GTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCT  >  Exported/2057‑2355
                                                                                                                                                     |                                                                                                                                                     
  ttgtttCCGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTCCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCt                                                                                                                                                     <  2:5154951/147‑1 (MQ=255)
    cTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCa                                                                                                                                                   >  1:2840795/1‑150 (MQ=255)
    cTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCa                                                                                                                                                   >  2:4303660/1‑150 (MQ=255)
       tACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCAtct                                                                                                                                                >  1:2080709/1‑150 (MQ=255)
        aCGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCAtctc                                                                                                                                               <  2:4202825/150‑1 (MQ=255)
             cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGtctc                                                                                                                                          <  1:1723961/150‑1 (MQ=255)
             cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGtctc                                                                                                                                          >  1:2205455/1‑150 (MQ=255)
               ttATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCtt                                                                                                                                        <  2:5723697/150‑1 (MQ=255)
                    aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTatat                                                                                                                                   >  1:1593565/1‑150 (MQ=255)
                    aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTatat                                                                                                                                   >  2:4777066/1‑150 (MQ=255)
                    aaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTatat                                                                                                                                   >  2:4969825/1‑150 (MQ=255)
                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTCGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCCTCAAATTTCTTCCTCATCTCGTCTCTTTATATc                                                                                                                                  >  1:5057100/1‑150 (MQ=255)
                       ccGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtt                                                                                                                                >  2:3998625/1‑150 (MQ=255)
                        cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCttt                                                                                                                               >  2:104386/1‑150 (MQ=255)
                        cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCttt                                                                                                                               <  2:1834402/150‑1 (MQ=255)
                           gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCtttatt                                                                                                                            <  2:4860139/150‑1 (MQ=255)
                            aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCtttattt                                                                                                                           >  2:2859523/1‑150 (MQ=255)
                            aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                           >  1:2632635/1‑150 (MQ=255)
                            aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                           <  1:4838003/150‑1 (MQ=255)
                            aaaCATTTTTACATCGATAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGACAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                           <  2:1733939/150‑1 (MQ=255)
                             aaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCtttatttt                                                                                                                          <  1:3413714/150‑1 (MQ=255)
                              aCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCtttatttta                                                                                                                         <  2:5631880/150‑1 (MQ=255)
                                  ttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGGTTTGTGGAAGTTTTGCCACAACAGTTGCTTTATTTACAATCTTTTATTTATTAGCCGGCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCAt                                                                                                                     >  1:1853144/1‑150 (MQ=255)
                                  ttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCAt                                                                                                                     >  1:4947347/1‑150 (MQ=255)
                                     tACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAg                                                                                                                 >  1:1356394/1‑150 (MQ=255)
                                      aCATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAg                                                                                                                 <  1:5270486/150‑1 (MQ=255)
                                       cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATANCTTTATTTTATCATAAAgg                                                                                                                >  2:5824192/1‑150 (MQ=255)
                                       cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGAAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                                >  1:3959066/1‑150 (MQ=255)
                                        aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                               >  1:5739671/1‑150 (MQ=255)
                                            ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGCAATTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTTTTTATATCTTTATTTTATCATAAAGGAGTAt                                                                                                           <  1:1000530/150‑1 (MQ=255)
                                              aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAttt                                                                                                         <  2:2262810/150‑1 (MQ=255)
                                                aaGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGa                                                                                                       >  2:276541/1‑150 (MQ=255)
                                                aaGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGa                                                                                                       >  1:5849789/1‑150 (MQ=255)
                                                 aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGaa                                                                                                      <  2:5263968/150‑1 (MQ=255)
                                                  gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                                                                                     <  1:2313995/150‑1 (MQ=255)
                                                   tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAcc                                                                                                    <  1:2728326/150‑1 (MQ=255)
                                                    gCAAGTCAAGTTATATGTATCTATAATGATTTGTGTAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                                   >  1:419435/1‑150 (MQ=255)
                                                    gCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                                                                                                   <  1:1966545/150‑1 (MQ=255)
                                                      aagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTc                                                                                                 <  2:1624541/150‑1 (MQ=255)
                                                      aagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGATTTATTTACAATCTTTTTTCTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAAGAGTATTTGAACCGTc                                                                                                 >  1:1127345/1‑150 (MQ=255)
                                                          caagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgc                                                                                             >  1:1239639/1‑150 (MQ=255)
                                                            agtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGACAAATTTCTCTCTCATCTCTTCTCTTTATATCTTTATTTTATCATAAAAGAGTATTTGAACCCTCGCGCgg                                                                                           >  1:4716132/1‑150 (MQ=255)
                                                                 tatGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTCATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGGATTTGAACCGTCGCGCGCGACAg                                                                                      >  2:5546970/1‑150 (MQ=255)
                                                                  atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAgg                                                                                     >  1:833223/1‑150 (MQ=255)
                                                                  atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAgg                                                                                     <  1:5163515/150‑1 (MQ=255)
                                                                    gTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTGCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGGGGGACAGGtt                                                                                   >  1:3053654/1‑150 (MQ=255)
                                                                       tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                                <  2:3145914/150‑1 (MQ=255)
                                                                          ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                             <  1:3522831/150‑1 (MQ=255)
                                                                           taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATa                                                                            <  2:5357887/150‑1 (MQ=255)
                                                                            aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                           >  2:3041498/1‑150 (MQ=255)
                                                                            aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                           >  2:3728217/1‑150 (MQ=255)
                                                                             aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAgg                                                                          >  2:410410/1‑150 (MQ=255)
                                                                             aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAgg                                                                          >  1:2534103/1‑150 (MQ=255)
                                                                               gATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGa                                                                        >  2:902577/1‑150 (MQ=255)
                                                                                 tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGata                                                                      <  2:3241121/150‑1 (MQ=255)
                                                                                  ttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGatat                                                                     <  2:1528168/150‑1 (MQ=255)
                                                                                   tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtt                                                                    <  1:5620803/150‑1 (MQ=255)
                                                                                   tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtt                                                                    <  2:788374/150‑1 (MQ=255)
                                                                                     tgGAAGTTTTGCCGCATCAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCTTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTTTTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtttt                                                                  <  2:1952019/150‑1 (MQ=255)
                                                                                     tgGAAGTCTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtttt                                                                  <  1:3178452/150‑1 (MQ=255)
                                                                                      ggAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                                 <  1:3748564/150‑1 (MQ=255)
                                                                                      ggAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                                 >  2:390663/1‑150 (MQ=255)
                                                                                         aGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTg                                                              >  1:5377270/1‑150 (MQ=255)
                                                                                          gTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGa                                                             <  2:4584486/150‑1 (MQ=255)
                                                                                          gTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGa                                                             >  2:3437932/1‑150 (MQ=255)
                                                                                           ttttGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaa                                                            <  2:684581/150‑1 (MQ=255)
                                                                                            tttGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaat                                                           <  2:1336238/150‑1 (MQ=255)
                                                                                               gCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat                                                        >  1:2421856/1‑150 (MQ=255)
                                                                                                 cacaACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTg                                                      >  2:1433446/1‑150 (MQ=255)
                                                                                                   caacaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGAt                                                    <  2:2363938/150‑1 (MQ=255)
                                                                                                    aacaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg                                                   <  2:114478/150‑1 (MQ=255)
                                                                                                      caGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGt                                                 >  2:3531972/1‑150 (MQ=255)
                                                                                                       aGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGta                                                <  2:5057100/150‑1 (MQ=255)
                                                                                                       aGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGta                                                >  2:2947596/1‑150 (MQ=255)
                                                                                                         ttGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGtata                                              <  1:949693/150‑1 (MQ=255)
                                                                                                          tGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATaa                                             >  2:3824241/1‑150 (MQ=255)
                                                                                                            gTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGCGATATTTTATTGAATAATTGATGGTATAAgg                                           >  1:2943682/1‑150 (MQ=255)
                                                                                                            gTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                           >  2:4487550/1‑150 (MQ=255)
                                                                                                             tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAggg                                          >  1:439607/1‑150 (MQ=255)
                                                                                                               tatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgggtc                                        >  2:215720/1‑148 (MQ=255)
                                                                                                                 tttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACtt                                      >  1:1475340/1‑150 (MQ=255)
                                                                                                                         cTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCtt                              <  2:1243329/150‑1 (MQ=255)
                                                                                                                            ttttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGa                           <  1:717734/150‑1 (MQ=255)
                                                                                                                               tttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTGTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAg                        <  1:329087/150‑1 (MQ=255)
                                                                                                                                ttattaGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGt                       <  2:4685898/150‑1 (MQ=255)
                                                                                                                                   ttaGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTCTTTGAACCGTCGCGCGGGATAGGTTTTTGTTAGGGATATTTTATTGAATAATTGATGGTATTAGGGACTTTCATGTTTGGAAAGTggg                    <  2:2566488/150‑1 (MQ=255)
                                                                                                                                   ttaGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTggg                    >  2:3339262/1‑150 (MQ=255)
                                                                                                                                    taGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTgggg                   <  2:205090/150‑1 (MQ=255)
                                                                                                                                       ccGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGAtt                >  1:5490885/1‑150 (MQ=255)
                                                                                                                                        cGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGAGGGGATTa               <  2:5454845/150‑1 (MQ=255)
                                                                                                                                           cAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGa            >  1:4616222/1‑150 (MQ=255)
                                                                                                                                           cAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGa            >  2:2458819/1‑150 (MQ=255)
                                                                                                                                            aaaTTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGaa           >  1:4072849/1‑150 (MQ=255)
                                                                                                                                             aaTTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAt          <  1:3642631/150‑1 (MQ=255)
                                                                                                                                               tttCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTa        <  1:4521502/150‑1 (MQ=255)
                                                                                                                                                ttcttcCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAg       >  2:2658195/1‑150 (MQ=255)
                                                                                                                                                ttCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAg       >  2:4396587/1‑150 (MQ=255)
                                                                                                                                                  cttccTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGAt     >  1:3859774/4‑150 (MQ=255)
                                                                                                                                                  ctcCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGAt     >  2:3642436/1‑150 (MQ=255)
                                                                                                                                                   ttccTCACCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATg    <  1:4296381/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCT  >  Exported/2057‑2355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑