Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 52,896 T→C 10.1% V45A (GTT→GCT)  U712_00225 → Protein veg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00688152,8960TC10.1% 1331.5 / 119.8 471V45A (GTT→GCT) U712_00225Protein veg
Reads supporting (aligned to +/- strand):  ref base T (195/225);  new base C (18/30);  total (216/255)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 494 total aligned reads displayed.

TAGTGAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCTTTTGTTTA  >  CP006881/52747‑53045
                                                                                                                                                     |                                                                                                                                                     
tAGTGAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGt                                                                                                                                                       <  2:4749404/150‑1 (MQ=255)
 aGTTAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGtt                                                                                                                                                      >  1:1465212/1‑150 (MQ=255)
  gTGAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGttt                                                                                                                                                     <  2:1759865/150‑1 (MQ=255)
   tGAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGtttt                                                                                                                                                    <  2:714050/150‑1 (MQ=255)
    gAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGttttt                                                                                                                                                   <  2:1681130/150‑1 (MQ=255)
    gAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGttttt                                                                                                                                                   <  2:2493195/150‑1 (MQ=255)
     aGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTtg                                                                                                                                                  <  2:2790619/150‑1 (MQ=255)
     aGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTtg                                                                                                                                                  <  1:3937116/150‑1 (MQ=255)
             gCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACaa                                                                                                                                          >  2:3946986/1‑150 (MQ=255)
              cAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAAc                                                                                                                                         >  2:5584916/1‑150 (MQ=255)
               atgggCGAAGACGTTGTCCAAATTTAAAAGCTCGCTGGATGGAAATTTAGGTAAAAGACTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACt                                                                                                                                        <  2:1095337/147‑1 (MQ=255)
               aaTGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACt                                                                                                                                        >  2:581854/1‑150 (MQ=255)
               aaTGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACt                                                                                                                                        >  1:4280829/1‑150 (MQ=255)
               aaTGGCGAAGACGTTGTCCGATATAAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACt                                                                                                                                        <  1:2713852/150‑1 (MQ=255)
                        gACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGa                                                                                                                               <  2:4795582/150‑1 (MQ=255)
                          cGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACg                                                                                                                             >  1:878990/1‑150 (MQ=255)
                           gTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGGAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAGAACGATTGAGCGTTCGGGCATTTTAGCTGGGACGTACCCTTCTGGTTTTGTGATACAACTAGATCAAGACga                                                                                                                            >  2:830877/1‑150 (MQ=255)
                                ccGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACt                                                                                                                       >  2:3173766/1‑150 (MQ=255)
                                 cGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTc                                                                                                                      <  2:5699155/150‑1 (MQ=255)
                                  gATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAAATAGATCAAGACGAGAACTCg                                                                                                                     >  1:4594729/1‑150 (MQ=255)
                                  gATATTAAAAAATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCg                                                                                                                     >  2:2125002/1‑150 (MQ=255)
                                    tatTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGtt                                                                                                                   <  2:1489247/150‑1 (MQ=255)
                                           aGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAaga                                                                                                            >  1:155112/1‑150 (MQ=255)
                                            gATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAagag                                                                                                           <  1:3691083/150‑1 (MQ=255)
                                             aTCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGt                                                                                                          <  2:3483953/150‑1 (MQ=255)
                                             aTCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGGTTGAAAGAGt                                                                                                          >  2:121143/1‑150 (MQ=255)
                                              tCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGtt                                                                                                         >  1:539155/1‑150 (MQ=255)
                                                  ttGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCAt                                                                                                     >  2:4599086/1‑150 (MQ=255)
                                                  ttGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCAt                                                                                                     <  2:2230785/150‑1 (MQ=255)
                                                  gtGATGGGAATTGAGGTAAAACTCTGAGGATAAAAGGAAAGGGTGCCCGCTGAAACACGATTGAGCGTTCGGGCATTTTAGCTGAGCCGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACCAGAACTCGTTTGAAAGAGTTTCAt                                                                                                     <  2:305782/149‑1 (MQ=255)
                                                    gATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTAGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATAc                                                                                                   <  1:4535100/150‑1 (MQ=255)
                                                    gATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACAATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATAc                                                                                                   >  1:2031096/1‑150 (MQ=255)
                                                      tGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAg                                                                                                 <  1:284082/150‑1 (MQ=255)
                                                       gggAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGt                                                                                                >  2:4385239/1‑150 (MQ=255)
                                                          aaTTTAGGTAAAAGGCTGACGTTAAAAGCACACGGTGGCCGCCGAAAACCGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTCCTGTTCTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTGCATACAGTTAt                                                                                             <  2:3396949/150‑1 (MQ=255)
                                                          aaTTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTAt                                                                                             >  1:2895731/1‑150 (MQ=255)
                                                           aTTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATg                                                                                            <  2:5187902/150‑1 (MQ=255)
                                                               aGGAAAAAGGCTGACGTTAAAACGAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGa                                                                                        <  1:769935/150‑1 (MQ=255)
                                                                ggTAAAAGCCTGACGTTAAAACCAAACGGTGCCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGat                                                                                       <  1:4059747/150‑1 (MQ=255)
                                                                  tAAAAGCCTGACGTTAAAAGCAAACGGTGTCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGatat                                                                                     <  2:1072336/150‑1 (MQ=255)
                                                                   aaaaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAtt                                                                                    <  2:3910841/150‑1 (MQ=255)
                                                                    aaaGGCTGACGTTAATAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAttt                                                                                   >  2:2625510/1‑150 (MQ=255)
                                                                     aaGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATAtttt                                                                                  <  1:4743116/150‑1 (MQ=255)
                                                                      aGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTg                                                                                 >  2:1890604/1‑150 (MQ=255)
                                                                        gCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAc                                                                               <  1:1216781/150‑1 (MQ=255)
                                                                         cTGACGTTAAAAGCAACCGGTGTCCGCGGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTGCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACt                                                                              <  1:2258111/150‑1 (MQ=255)
                                                                         cTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACt                                                                              >  2:4157987/1‑150 (MQ=255)
                                                                          tGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAAATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGAGTg                                                                             >  1:2439052/1‑150 (MQ=255)
                                                                          tGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCATTGTGTTTTTGTGATACAACTAGATGAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTg                                                                             >  1:4386623/1‑150 (MQ=255)
                                                                          tGACGTTAAAAGCAAACGGTGAGCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTg                                                                             >  1:1292609/1‑150 (MQ=255)
                                                                            aCGTTAAAAGCACACGGTGGCCGCCGAAAAACGATTGAGCGTCCGGGCATTTCAGCTGAGACGTACCGTTCTGTCTTTGTGATACAACTAGATCAAGAGGAGAACTCGTTTGAAAGAGTTTCATAGAGTTATGCTGATATTTTGACTgag                                                                           <  1:3185435/150‑1 (MQ=255)
                                                                             cGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTgaga                                                                          >  1:5699090/1‑150 (MQ=255)
                                                                              gTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATGTTTTGACTGAGAc                                                                         >  2:1427870/1‑150 (MQ=255)
                                                                              gTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGAc                                                                         >  2:3825483/1‑150 (MQ=255)
                                                                              gTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTAAGACGTACCCTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGTCTGTGAc                                                                         >  1:784415/1‑150 (MQ=255)
                                                                               ttAAAAGCAAACGGTGTCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACt                                                                        <  1:1932714/150‑1 (MQ=255)
                                                                                tAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTg                                                                       >  1:1024061/1‑150 (MQ=255)
                                                                                  aaaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGtt                                                                     <  1:2133753/150‑1 (MQ=255)
                                                                                  aaaGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGtt                                                                     >  2:2404701/1‑150 (MQ=255)
                                                                                     gCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACANTTATGCTGATATTTTGACTGAGACTGTTgtg                                                                  >  1:1395950/1‑148 (MQ=255)
                                                                                         aCGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGa                                                              <  1:2313379/150‑1 (MQ=255)
                                                                                          cGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGAc                                                             >  2:4002495/1‑150 (MQ=255)
                                                                                           ggTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACa                                                            <  2:496331/150‑1 (MQ=255)
                                                                                                    cgAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAatgat                                                   >  1:3499558/1‑150 (MQ=255)
                                                                                                     gAAAAATGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAatgatg                                                  >  2:4884589/1‑150 (MQ=255)
                                                                                                     gAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAatgatg                                                  >  1:5653772/1‑150 (MQ=255)
                                                                                                     gAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTG‑TTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAAtgatga                                                 >  1:3787797/1‑150 (MQ=255)
                                                                                                      aaaaaCGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAAtgatga                                                 >  1:5521114/1‑150 (MQ=255)
                                                                                                       aaaaCGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGAc                                                <  1:4160324/150‑1 (MQ=255)
                                                                                                           cGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGAcgccg                                            <  1:1871748/150‑1 (MQ=255)
                                                                                                           cGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCCTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGAcgccg                                            >  1:481053/1‑150 (MQ=255)
                                                                                                              ttGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCaa                                         <  2:778164/150‑1 (MQ=255)
                                                                                                               tGAGCGTTCGGGCATTTTAGCTGAGACCTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAg                                        <  1:3020749/150‑1 (MQ=255)
                                                                                                                   cGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCa                                    <  2:4461303/150‑1 (MQ=255)
                                                                                                                     ttCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGAATTGATCTCTATTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGt                                  <  1:581374/150‑1 (MQ=255)
                                                                                                                         ggCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCa                              >  2:5295748/1‑150 (MQ=255)
                                                                                                                          gCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAt                             >  2:1543611/1‑150 (MQ=255)
                                                                                                                           cATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt                            <  1:642183/150‑1 (MQ=255)
                                                                                                                           cATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt                            <  2:4234606/150‑1 (MQ=255)
                                                                                                                           cATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt                            <  1:1758953/150‑1 (MQ=255)
                                                                                                                           cATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTAACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAtt                            <  1:3272026/150‑1 (MQ=255)
                                                                                                                            aTTTTAGCTGAGAGGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGACGACTCGTTTGAAAGAGTTTCAGACAGTTATGGTGATATTTTGACTGAGACTGTTGTGCTGACATTTAATGATGACGGCGCAAGCTCAGTGGCAttt                           >  1:4999809/1‑150 (MQ=255)
                                                                                                                            aTTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCAttt                           <  2:4109374/150‑1 (MQ=255)
                                                                                                                              tttAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTa                         <  1:2518602/150‑1 (MQ=255)
                                                                                                                                tAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAAc                       <  1:4914802/150‑1 (MQ=255)
                                                                                                                                   cTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg                    <  2:2935404/150‑1 (MQ=255)
                                                                                                                                   cTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg                    >  2:4480409/1‑150 (MQ=255)
                                                                                                                                   cTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACggg                    <  2:2046758/150‑1 (MQ=255)
                                                                                                                                      agaCGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAg                 >  1:2666812/1‑150 (MQ=255)
                                                                                                                                      agaCGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAg                 >  1:3366064/1‑150 (MQ=255)
                                                                                                                                          gTACCCTCCTGTTTTTCTGATACAACAAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTTACGGGCAGTGaa             <  2:511828/150‑1 (MQ=255)
                                                                                                                                             cccTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCt          <  1:4467544/150‑1 (MQ=255)
                                                                                                                                             cccTTCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCt          >  2:186514/1‑150 (MQ=255)
                                                                                                                                              ccTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCtt         >  1:5742398/1‑150 (MQ=255)
                                                                                                                                               cTTCTGTTTTTTTGATACACCTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCttt        <  2:2570068/150‑1 (MQ=255)
                                                                                                                                                ttCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCtttt       >  2:2833101/1‑150 (MQ=255)
                                                                                                                                                ttCTGCTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCtttt       <  1:5174049/150‑1 (MQ=255)
                                                                                                                                                  cTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCTTTTGt     <  2:4572490/150‑1 (MQ=255)
                                                                                                                                                  cTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCTTTTGt     <  2:2716754/150‑1 (MQ=255)
                                                                                                                                                     tttttGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCTTTTGTTTa  >  1:1121042/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGTGAGGTGGATGCAATGGCGAAGACGTTGTCCGATATTAAAAGATCGCTTGATGGGAATTTAGGTAAAAGGCTGACGTTAAAAGCAAACGGTGGCCGCCGAAAAACGATTGAGCGTTCGGGCATTTTAGCTGAGACGTACCCTTCTGTTTTTGTGATACAACTAGATCAAGACGAGAACTCGTTTGAAAGAGTTTCATACAGTTATGCTGATATTTTGACTGAGACTGTTGAGCTGACATTTAATGATGACGCCGCAAGCTCAGTGGCATTTTAACGGGCAGTGAACCTTTTGTTTA  >  CP006881/52747‑53045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑