Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,909,199 C→T 10.8% G49E (GGA→GAA)  U712_14990 ← Biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,909,1990CT10.8% 1113.6 / 34.3 377G49E (GGA→GAA) U712_14990Biotin synthase
Reads supporting (aligned to +/- strand):  ref base C (124/204);  new base T (46/2);  total (170/207)
Fisher's exact test for biased strand distribution p-value = 1.33e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

GCTTCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATTCTC  >  CP006881/2909050‑2909348
                                                                                                                                                     |                                                                                                                                                     
  ttCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATAATGAGCTTTTCTTTTTTTTCg                                                                                                                                                     >  2:4111969/1‑150 (MQ=255)
      cgcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTaaa                                                                                                                                                 <  2:419703/150‑1 (MQ=255)
      cgcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTaaa                                                                                                                                                 <  1:5500856/150‑1 (MQ=255)
       gcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTaaaa                                                                                                                                                <  2:1593259/150‑1 (MQ=255)
        ccTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAg                                                                                                                                               <  2:2579086/150‑1 (MQ=255)
         cTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTTCGTAAAAgt                                                                                                                                              >  2:3473961/1‑150 (MQ=255)
          ttCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAgtg                                                                                                                                             <  1:5572301/150‑1 (MQ=255)
          ttCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAgtg                                                                                                                                             <  2:1041179/150‑1 (MQ=255)
           tCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAgtgt                                                                                                                                            <  1:4145757/150‑1 (MQ=255)
               gcagcNTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTTCGGGCAGAGCCCCGGTTTCGCATTCATAAACATATTGAGCTTTTATTTTTTTTCGTTAAAATGTTTTc                                                                                                                                        >  1:5814877/1‑150 (MQ=255)
                 agcGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTg                                                                                                                                      <  2:5698499/150‑1 (MQ=255)
                  gcGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCCAAATCGCAGACTGTGAACAATAGCCGCAGTTTCCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGa                                                                                                                                     <  2:1841988/150‑1 (MQ=255)
                       tCCTTATACACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGa                                                                                                                                <  1:1002099/150‑1 (MQ=255)
                        ccTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGGTTTTTCAATCAATATAAAATTGAGCTTTATTTTTTTTCTGTAAAAGTGTTTTGTTAtgtggt                                                                                                                               >  1:2150688/1‑148 (MQ=255)
                             ttCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAACCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGAATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGcc                                                                                                                          <  1:4591611/150‑1 (MQ=255)
                               cACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGcccc                                                                                                                        <  1:4307619/150‑1 (MQ=255)
                               cACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGcccc                                                                                                                        <  2:5464126/150‑1 (MQ=255)
                                aCCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCGGAGCCCGGATTTCGCATTCATAATCATATTAAGATTTACTTTTTTTTCTTTAAAGAGTTTTTTGTTGTGAAAAGacccc                                                                                                                       >  1:1364386/1‑149 (MQ=255)
                                  cATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTg                                                                                                                     <  2:2409360/150‑1 (MQ=255)
                                   aTCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGc                                                                                                                    <  1:3278387/150‑1 (MQ=255)
                                     ccGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCAt                                                                                                                  <  2:1395785/150‑1 (MQ=255)
                                      cGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGGCCGGATTTCGCATTCATTAACATATTGGGCTGTACTTTTTTTTCGTTAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCAtt                                                                                                                 >  2:451087/1‑150 (MQ=255)
                                      cGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCAtt                                                                                                                 <  2:2370140/150‑1 (MQ=255)
                                       ggTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTa                                                                                                                <  1:2085025/150‑1 (MQ=255)
                                         tAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGAATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAAt                                                                                                              <  1:5096378/150‑1 (MQ=255)
                                          aaGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCAAATTGAGCTTTACTTTTTTTCCTTTAAAAAGTTTTTTTGTGTTAAAAGGCCCCTGCCTTtata                                                                                                             >  2:3335511/1‑150 (MQ=255)
                                          aaGACTCAATCGGCCCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCGGAGCCCCGGTTTTGCCTTTTTAATTATATTTTACTTTTATTTTTTTTTCTAAAAAAGTTTTTTTTTGTGTAAAACTCCGGGCATTAAtt                                                                                                             >  1:4653289/1‑149 (MQ=255)
                                            gACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTGCGGGCAGAGCCCGGAATTCGCATTCATAATCATATTTGGGTTTAATTTTTTTTCGTGGAAACGGGTTCTGGTGTGAATAACCCCCTGGATTtatatc                                                                                                           >  2:4900168/1‑148 (MQ=255)
                                                 aaTCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAat                                                                                                      <  1:4111969/150‑1 (MQ=255)
                                                  aTCGGCTCTTTCGAATTCTCAGACTGTGAACAATAGCCACAGTTTTCCGGGCAGCGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAGAAGCCCCGTGCATTAATAGCAAAata                                                                                                     <  2:671519/150‑1 (MQ=255)
                                                  aTCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGGCCGGATTTCGCATTCATAATCATATTGTGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAata                                                                                                     >  1:5282357/1‑150 (MQ=255)
                                                  aTCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTATTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAata                                                                                                     <  1:1318761/150‑1 (MQ=255)
                                                   tCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAatat                                                                                                    <  1:3085987/150‑1 (MQ=255)
                                                       cgcTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtc                                                                                                <  1:4919079/150‑1 (MQ=255)
                                                       cgcTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtc                                                                                                <  2:1517288/150‑1 (MQ=255)
                                                        gcTTTCGAAATCGCAGACTGTGAACAATAGCGGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtca                                                                                               <  1:1962272/150‑1 (MQ=255)
                                                        gcTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtca                                                                                               <  1:3652810/150‑1 (MQ=255)
                                                         cTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTACGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTTCCTAAAAGTGTTTTCTTATGTGAAAAGCCCCGTGCATTAATAGCAAAAAATCTtcat                                                                                              >  1:5670397/1‑150 (MQ=255)
                                                          tttCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtcatc                                                                                             <  2:5102329/150‑1 (MQ=255)
                                                            tCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAg                                                                                           <  2:1185782/150‑1 (MQ=255)
                                                              gAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGTGCTTTACTTTTTTTCCGTAAAAATGTTTTTTGATGTGAAAAGCCCCGTGCATTAATAACAAAATATCTTCATCAGGa                                                                                         >  1:1519874/1‑150 (MQ=255)
                                                               aaaTCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGAc                                                                                        <  2:3820772/150‑1 (MQ=255)
                                                               aaaTCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGCCAGAGCCCGCATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGAc                                                                                        <  2:4583334/150‑1 (MQ=255)
                                                                   cGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATg                                                                                    <  2:937467/150‑1 (MQ=255)
                                                                    gCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGt                                                                                   <  2:3049767/150‑1 (MQ=255)
                                                                    gCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGt                                                                                   <  2:1130732/150‑1 (MQ=255)
                                                                      aGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGGTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTTCGTAAAAATGTTTTCTTTTGTGTAAAGACCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTaa                                                                                 >  2:4425514/1‑150 (MQ=255)
                                                                        aCTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAata                                                                               >  1:572358/1‑150 (MQ=255)
                                                                         cTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAatat                                                                              >  1:3919168/1‑150 (MQ=255)
                                                                          tgtgAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATAtt                                                                             >  1:5011268/1‑150 (MQ=255)
                                                                           gtgAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTg                                                                            <  1:4212479/150‑1 (MQ=255)
                                                                            tgATCAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCCCATACATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGa                                                                           <  2:1956367/150‑1 (MQ=255)
                                                                            tgAACAATAGCCGCAGTTTTCCGGGGAGAGCCCGGATTTCGCATTCATAAACATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGa                                                                           >  1:736502/1‑150 (MQ=255)
                                                                              aaCAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGaaa                                                                         <  1:276675/150‑1 (MQ=255)
                                                                                cAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAgc                                                                       <  2:3615527/150‑1 (MQ=255)
                                                                                  aTAGCCGCAGTTTTCCGGGCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAgcgc                                                                     <  1:3513633/150‑1 (MQ=255)
                                                                                  aTAGCCGCAGTTTTCCCGGCAGAGCCCGGATTTCGCCTTCATTATCATATTTAGCTTTTCTTTTTTTCCGTAAAAGTGTTTTCTCATGTGAAAAGCCCCGCGCATTAATAGCAAAATATCTTCATCAAGACAATGTAATAATGAAAgcgc                                                                     >  1:1104346/1‑150 (MQ=255)
                                                                                   tAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGcc                                                                    <  1:4869621/150‑1 (MQ=255)
                                                                                      ccGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAgc                                                                       >  1:891704/1‑144 (MQ=255)
                                                                                      ccGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAgc                                                                       <  2:891704/144‑1 (MQ=255)
                                                                                        gCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTc                                                               <  2:1752616/150‑1 (MQ=255)
                                                                                           gTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCgtc                                                            >  2:5012898/1‑150 (MQ=255)
                                                                                                ccGGGCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCgtcagtca                                                       <  2:2958547/150‑1 (MQ=255)
                                                                                                  gggCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACt                                                     <  1:2629293/150‑1 (MQ=255)
                                                                                                  gggCAGACCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACt                                                     <  2:5382910/150‑1 (MQ=255)
                                                                                                   ggCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACtt                                                    >  1:4840616/1‑150 (MQ=255)
                                                                                                     cAGAGCCCGAATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCt                                                  <  2:470151/150‑1 (MQ=255)
                                                                                                       gagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTgg                                                >  1:17399/1‑149 (MQ=255)
                                                                                                       gagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGc                                                >  2:2069073/1‑150 (MQ=255)
                                                                                                           ccGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTcca                                            <  1:537691/150‑1 (MQ=255)
                                                                                                            cGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccag                                           >  2:515554/1‑150 (MQ=255)
                                                                                                              gATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccagcc                                         >  1:3991995/1‑150 (MQ=255)
                                                                                                               aTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccagcca                                        >  1:5209903/1‑150 (MQ=255)
                                                                                                                tttCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccagccag                                       <  2:4651426/150‑1 (MQ=255)
                                                                                                                   cGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCAc                                    <  2:1470139/150‑1 (MQ=255)
                                                                                                                     cATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTTTTTTCTGATGTGAAAAGCCCCGTTCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAATGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCActc                                  <  2:5599418/150‑3 (MQ=255)
                                                                                                                     cATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCAccc                                  <  1:3473961/150‑1 (MQ=255)
                                                                                                                      aTTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCg                                 >  2:2802360/1‑150 (MQ=255)
                                                                                                                        tCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGt                               >  2:2525746/1‑150 (MQ=255)
                                                                                                                         cATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTc                              >  1:4763754/1‑150 (MQ=255)
                                                                                                                          aTAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCt                             <  2:5354133/150‑1 (MQ=255)
                                                                                                                          aTAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCt                             <  2:1301097/150‑1 (MQ=255)
                                                                                                                               cATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAg                        <  1:816003/150‑1 (MQ=255)
                                                                                                                               cATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGAACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAg                        >  1:270469/1‑150 (MQ=255)
                                                                                                                                atatTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGt                       >  1:1832024/1‑150 (MQ=255)
                                                                                                                                  atTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtc                     >  2:3997953/1‑150 (MQ=255)
                                                                                                                                   ttGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcc                    <  2:2179708/150‑1 (MQ=255)
                                                                                                                                    tGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcca                   >  2:1488874/1‑150 (MQ=255)
                                                                                                                                    tGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcca                   >  1:64512/1‑150 (MQ=255)
                                                                                                                                    tGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcca                   >  1:3002072/1‑150 (MQ=255)
                                                                                                                                     gAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAGGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccat                  <  2:2632702/150‑1 (MQ=255)
                                                                                                                                       gCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCTCCTCTTCGTCAGTCACTTGGTCTCCAGCCAGCACCCGGTCTGCGAGTtccatcc                >  1:1607125/1‑150 (MQ=255)
                                                                                                                                       gCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccatcc                <  1:1053862/150‑1 (MQ=255)
                                                                                                                                        cTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccatcca               <  2:2150688/150‑1 (MQ=255)
                                                                                                                                         tttACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGAGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATAGAATGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTccatccat              >  1:3760018/1‑150 (MQ=255)
                                                                                                                                            aCTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGa           >  2:3277915/1‑150 (MQ=255)
                                                                                                                                               ttttttCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCGCTACTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGattc        >  1:1760017/1‑150 (MQ=255)
                                                                                                                                                    tCCGTAAAAGTGTTTTCTGATGTGAAAAGCTCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGTCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATtct   <  1:2573722/150‑1 (MQ=255)
                                                                                                                                                     ccGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATATCAACATATCTTCATCAGGACAATGTAATATTGTATGCGCCTCTTCGTCAGACACTTCTGCTCCAACCAGCGCCCGGACTGCGAGTTCCATCCATTGATTCATtctc  <  1:3365992/150‑1 (MQ=255)
                                                                                                                                                     ccGTAAAAGTGTTTTCTGATGTGAAAAAACCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATATAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGTTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTCATTTATtctt  >  1:237253/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATTCTC  >  CP006881/2909050‑2909348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑