BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,350,137	C→A	7.1%	P59P (CCC→CCA)	U712_17215 →	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,350,137	0	C	A	7.1%	506.0 / 7.2	290	P59P (CCC→CCA)	U712_17215	Hypothetical Protein
Reads supporting (aligned to +/- strand): ref base C (119/103); new base A (27/5); total (155/135)
Fisher's exact test for biased strand distribution p-value = 9.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

CCATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGCAAA  >  CP006881/3349989‑3350286

 cATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCgg                                                                                                                                                      <  2:273647/150‑1 (MQ=255)

 cATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCgg                                                                                                                                                      <  2:5457926/150‑1 (MQ=255)

 cATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCgg                                                                                                                                                      <  1:5594430/150‑1 (MQ=255)

  aTTGTTGTGGCAGCTTGTATCATTATTTTGTCGGTCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGa                                                                                                                                                     >  1:3260945/1‑150 (MQ=255)

     gttgtGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGaaaa                                                                                                                                                  >  1:783729/1‑150 (MQ=255)

        gtgGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAagag                                                                                                                                               <  1:2148614/150‑1 (MQ=255)

          ggCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGaa                                                                                                                                             <  1:5832557/150‑1 (MQ=255)

           gCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAg                                                                                                                                            <  2:4428795/150‑1 (MQ=255)

             aGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTa                                                                                                                                          >  2:2162753/1‑150 (MQ=255)

             aGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTa                                                                                                                                          <  2:4950725/150‑1 (MQ=255)

              gCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACCGGAAAAGAGAAGTaa                                                                                                                                         >  1:3171511/1‑150 (MQ=255)

               cTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAAt                                                                                                                                        <  2:1375251/150‑1 (MQ=255)

               cTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAAt                                                                                                                                        >  2:5405504/1‑150 (MQ=255)

                tgttATCATTTTTTTGTGGGCCGTGGTATGTCTTCGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATCCTCAGCAAAAAGAAAACCGGGAAAAGAGAAGTAAtt                                                                                                                                       <  1:3539207/147‑1 (MQ=255)

                  gTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATtct                                                                                                                                     <  2:4741421/150‑1 (MQ=255)

                   tATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATtctc                                                                                                                                    <  2:2780904/150‑1 (MQ=255)

                   tATCATTATTTTGTCGGCCGTGGTATGTTTACGAGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACAAGAAAAAAGAAGTaaatata                                                                                                                                    >  1:1407393/1‑145 (MQ=255)

                     tCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGAGTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACAGGGAAAAGGGAAGTAATTATCtt                                                                                                                                  >  1:1925225/1‑150 (MQ=255)

                      cATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACAAGGAAAGAGAAATAATTCTCttt                                                                                                                                 >  1:541382/1‑150 (MQ=255)

                       attattTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCtttt                                                                                                                                <  1:1215795/150‑1 (MQ=255)

                             ttGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTAATTCTCTTTTCCGGGt                                                                                                                          >  2:1703468/1‑150 (MQ=255)

                             ttGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGt                                                                                                                          <  2:4552224/150‑1 (MQ=255)

                              tGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGtt                                                                                                                         >  2:2078365/1‑150 (MQ=255)

                                      ttgttATGTTTAGGTGATACACTTTTTTCACAGCTTTTGCGTTGGTCTGCCATCATACTGTTTTTCGCGGGAATTTTGTTGTTCCCCGCATACTCAGAAAAAAGAAAACCCGGAAAAGGAAAGTAATTTTTTTTTCCTTGTTTTTATGtt                                                                                                                 <  1:2901640/146‑1 (MQ=255)

                                       tgttATGTTAACGGGACACAGTTTATAACCAGCCTCTCCGCTGGCCTGGCACCATACTGTTTTCCGGGGGATTTGTGTTGCTCCCCCCAACCCAAAAAAAAAAAAAACCCGAAAAAGAGAATTATTTCTCTTTTCCGGGTTTTTATGttt                                                                                                                <  2:2882349/147‑1 (MQ=255)

                                        ggTAGGTTTACGGGCCCCAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTCCGCGGGATTTGTGATGGTCCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAATTAATTCTCTTTTCCGGGTTTTTATGTtta                                                                                                               <  1:3072727/150‑1 (MQ=39)

                                           aTGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGTATTGTGATGGTTCCCGCATAATCAGCAAAAAGAAAACCAGGAAAAGAGAAGTAAATTTCTTTTTCTGGTTTTTATGTttatta                                                                                                            >  1:1784855/1‑150 (MQ=255)

                                           aTGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTCCCCGCATACACAGCAAAAAGAAAACCGGGAAAAGAGAAGTAATTCTCTTTCCCGGGTTTTTATGTttatta                                                                                                            <  2:3397200/150‑1 (MQ=255)

                                            tGTTTAGGGGACACAGTCTTATACCAGCCTATGCGCTGGTTTGGCTACATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATCCTAAGCAAAAAGAAAACCCGGAAAAGAGAGGTATTTCTCTTTTCCGGGTTTTTATGTTtattat                                                                                                           <  1:2167444/150‑1 (MQ=255)

                                               ttAGGGNACACACTTTATTACCAGCTATTGCGCTGGACTGGCATCTTACTGTTTTCCGCGGGAATTGTGTTGGTTCCCCAATCCTCAGAAAAAAAAAAACCGGAAAAAGAGAAGTAATTCTCTTTTCTGTGTTTTTATGTTTATTATAAt                                                                                                        <  1:3227157/150‑1 (MQ=255)

                                                    ggACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACAAGGAAAAGAGAAGTAATTCTCTTTTTCGGGTTTTTATGTTTATTATAATCtttc                                                                                                   >  1:1524748/1‑148 (MQ=255)

                                                         caGTCTAATACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAGGCGGAAAAGAGAAATAAATCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCcgccc                                                                                              >  2:1073003/1‑148 (MQ=255)

                                                          aGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCcgcgcg                                                                                             >  1:5070885/1‑150 (MQ=255)

                                                          aGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGAGTTTTTATGTTTATTATAATCTTCCcgcgcg                                                                                             <  1:5431569/150‑1 (MQ=255)

                                                              ttttACCAGCCTATCCGCTGGCCGGGCACCATACTGTTTTTCGCGGGAATTGTGTTGGTTCCCGCATACTCAGCAAAAAGAAAACCGGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAata                                                                                         <  2:5183982/148‑1 (MQ=255)

                                                              tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCGGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAata                                                                                         >  1:5675679/1‑150 (MQ=255)

                                                              tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCCCGCGAata                                                                                         >  2:3884140/1‑150 (MQ=255)

                                                               tttACCAGCGTATGCGCTGGACTGGCATCATACTGTTTTCCGCGGGAATTGTGATGGTTCCCCCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTGTTTCCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAata‑t                                                                                       <  2:5302538/149‑1 (MQ=255)

                                                                ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACACGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCCCGCGAATA‑Tc                                                                                      >  2:5692914/1‑150 (MQ=255)

                                                                 tACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACTCGGAAAAGAGAAGAAATTCTCTTTTCCGGGTTTTTATGTTTATTATAAATTTCCCGCGCGAATA‑TCg                                                                                     >  1:162159/1‑150 (MQ=255)

                                                                  aCCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAGCCGGAAAAGAGAAGTAATTCTCTTTTCCTGGTTTTTATGTTTTTTATAATCTTTCCCCGCGAATA‑TCGt                                                                                    >  1:1840996/1‑150 (MQ=255)

                                                                   ccAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGGGAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTc                                                                                   >  1:300091/1‑150 (MQ=255)

                                                                     aGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACGCGGAAAAGAGAAGTAATTCTCTTTTCCGGGGTTTTATGTTTATTATAAACTTCCCGCGCCAAAA‑TCGTCTg                                                                                 >  1:4041078/1‑150 (MQ=255)

                                                                         tATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCTGGGAAAGAGAAGTAATTCTCTTTTCTGGGTTTTTATGTTTATTATTATCTTCCCGCCCGAATA‑TCGTCTGTCAt                                                                             >  1:3426672/1‑150 (MQ=255)

                                                                         tATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCAt                                                                             >  2:711359/1‑150 (MQ=255)

                                                                          aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGGAATTCTCTTTTCCGGGGTTTTATGTTTATTATAATCTTCCCGCCCGAATA‑TCGTTTGTCAtt                                                                            >  1:4885861/1‑150 (MQ=255)

                                                                          aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCCCATACTCAGCAAAAAAAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCTGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCAtt                                                                            <  2:1020088/150‑1 (MQ=255)

                                                                            gcgtTGGATTGGCATCTTACTGTTTTTCCCGGAATTTGTGATGTTTCCAGCATACACACCAAAAAGAAAACCCAAAAAAGAGAAGTAATTCTCTTTTCAGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTcc                                                                          <  1:5275188/150‑1 (MQ=255)

                                                                            gcgcTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGGCAtttc                                                                          >  1:1956029/1‑148 (MQ=255)

                                                                             cgcTGGACTGGCATCATACTGTTTTTCGCGGGATTTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCGGGAAAAGAGAAGTAATTCTCTTTTCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTccc                                                                         <  2:4021969/150‑1 (MQ=255)

                                                                                 ggACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGGGAAAGAGAAGAAAATCTTTTTTTCCGGTTTTTTTGTTTTTTATTATCTTTTCCCCCGAATA‑TTGTGTGTTCTTCCCTCaa                                                                     >  1:2673678/1‑149 (MQ=255)

                                                                                  gACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATa                                                                    >  2:319299/1‑150 (MQ=255)

                                                                                     tGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATaaag                                                                 >  1:3851461/1‑148 (MQ=255)

                                                                                        catcatACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGc                                                              >  1:2025629/1‑150 (MQ=255)

                                                                                         atcatACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTCCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGcc                                                             <  2:783729/150‑1 (MQ=255)

                                                                                           catACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCtt                                                           >  2:4344255/1‑150 (MQ=255)

                                                                                           catACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACAAGGAAAAGAGAAGTAATTCTCTTTTCTGGGTTTTTATGTTTATTATAATTTTCCCACGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCtt                                                           >  1:4236266/1‑150 (MQ=255)

                                                                                            atACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCGGGAAAAGAGAAAGAAATCTCTTTTTCGGGTTTTTATGTTTTTTTTAATCTTTCCCCGGGAATATTGGTTTTTCATT‑CCTCATAAGGaagcctc                                                          >  1:1053939/1‑144 (MQ=255)

                                                                                            atACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGCATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTc                                                          >  2:5255157/1‑150 (MQ=255)

                                                                                              aCTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGa                                                        >  2:4497668/1‑150 (MQ=255)

                                                                                                 gTTTTTCGCGGGAATTGTGATGGTCCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAAt                                                     <  2:266701/150‑1 (MQ=255)

                                                                                                  tttttCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATc                                                    >  1:4431972/1‑150 (MQ=255)

                                                                                                    ttccgcgGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCAc                                                  <  1:1358329/147‑1 (MQ=255)

                                                                                                          gggAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAAACTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCActtcttg                                            >  2:1989813/1‑149 (MQ=255)

                                                                                                          gggAATTGTGATGGTCCCCGCATCCCCAGCAAAAAGAAAACCCGAAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                            <  2:3219764/150‑1 (MQ=255)

                                                                                                            gAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGt                                          >  2:1825201/1‑150 (MQ=255)

                                                                                                              aTTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTg                                        >  2:2460973/1‑150 (MQ=255)

                                                                                                               ttGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGt                                       >  2:3697006/1‑150 (MQ=255)

                                                                                                                  tgATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGa                                    <  2:3559351/150‑1 (MQ=255)

                                                                                                                  tgATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGa                                    >  1:5529907/1‑150 (MQ=255)

                                                                                                                    gtgttccccACATCCACAACAAAAAGAAACCCAGAAAAAGAGAATTAATCCTCTTTTCGGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACt                                  <  2:1153385/144‑1 (MQ=255)

                                                                                                                     tGGTTCCCGCATACTCAGCAAAAAGAAAACCCGAAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTc                                 <  2:2069887/150‑1 (MQ=255)

                                                                                                                      ggTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTcc                                >  1:2478854/1‑150 (MQ=255)

                                                                                                                          cccGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCa                            >  2:4809452/1‑150 (MQ=255)

                                                                                                                          cccGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCa                            >  2:833622/1‑150 (MQ=255)

                                                                                                                          cccGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCa                            >  1:5350260/1‑150 (MQ=255)

                                                                                                                           ccacATACACAGCAAAAAAAAACCCCGGAAAAGAGAAGTAATTCTTTTTCCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAt                           <  2:1624097/147‑1 (MQ=255)

                                                                                                                            cGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAtt                          >  2:419779/1‑150 (MQ=255)

                                                                                                                             gCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTTCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCTACTCCTTCAttt                         >  1:2380879/1‑150 (MQ=255)

                                                                                                                             gCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAttt                         >  2:3028173/1‑150 (MQ=255)

                                                                                                                             gCATACTCACCAAAAAGAAAACCCGGAAAAGAGAAGTATTTCTCTTTTCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAttt                         <  2:2039088/150‑1 (MQ=255)

                                                                                                                              aaTACACAGAAAAAAGAAAACACAGAAAAGAGAATTTATTCTCTTTATCATGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTACGAAATCACTTCTTCGTTGTCGACTCCTTCAtttt                        <  1:2078365/149‑1 (MQ=255)

                                                                                                                               aTACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAttttt                       >  1:4722707/1‑150 (MQ=255)

                                                                                                                                tACCCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATCCTTTTTCCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTc                      <  2:4852956/150‑1 (MQ=255)

                                                                                                                                  cTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAg                    >  1:4141956/1‑150 (MQ=255)

                                                                                                                                   tCAGCAAAAAGAAAACCGGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGa                   >  2:5759297/1‑150 (MQ=255)

                                                                                                                                   tCAGCAAAAAGAAAACCCGGAAAAGTGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGGCGACTCCTTCATTTTTCAGa                   >  1:367129/1‑150 (MQ=255)

                                                                                                                                    caccAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGaa                  >  2:2217887/4‑150 (MQ=255)

                                                                                                                                       cAAAAAAAAAACCAGAAAAAAAGAATTATTTCTCTTTCCCGTCTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATg               <  1:1550777/150‑1 (MQ=255)

                                                                                                                                          aaaaaaaaCCGGAAAAAGAGAAGTAATTCTCTTTCCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTaa            <  2:4102309/150‑1 (MQ=255)

                                                                                                                                          aaaGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTaa            >  1:974487/1‑150 (MQ=255)

                                                                                                                                            aGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAAtt          >  1:2261517/1‑150 (MQ=255)

                                                                                                                                             gAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAAttt         >  2:5025308/1‑150 (MQ=255)

                                                                                                                                             aaaacCCCGAAAAAGAGAAGTAATTCTCTTTTCCGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAAttt         <  2:4403274/149‑1 (MQ=255)

                                                                                                                                               aaaCCCGGAAAAGAGAATTAATTCTTTTTTCTGTGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTcc       <  2:1284053/150‑1 (MQ=255)

                                                                                                                                               aaaCCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTcc       >  2:1432506/1‑150 (MQ=255)

                                                                                                                                                aaCCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCg      >  2:2401986/1‑150 (MQ=255)

                                                                                                                                                   ccGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGCaa   >  1:4818794/1‑150 (MQ=255)

                                                                                                                                                    cGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGCaaa  >  1:4672410/1‑150 (MQ=255)

                                                                                                                                                    cGGAAAAGAGAAGAAATTCTCTTTTCCGGGGTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGGCTCCTTCATTTTTCAGAAATGGAAGTTCCGCaaa  >  2:4998713/1‑150 (MQ=255)

CCATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGAAAAGAGAAGTAATTCTCTTTTCCGGGTTTTTATGTTTATTATAATCTTCCCGCGCGAATA‑TCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGCAAA  >  CP006881/3349989‑3350286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`