Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,729 G→A 100% G181G (GGG→GGA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,7290GA99.8% 1333.8 / ‑5.1 380G181G (GGG→GGA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (172/206);  total (174/206)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 396 total aligned reads displayed.

TGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAAT‑GGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTT  >  CP006881/3723582‑3723875
                                                                                                                                                    |                                                                                                                                                  
tGAGGAGCTTTCTTTTCATTCTTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGc                                                                                                                                                  <  2:5228934/150‑1 (MQ=255)
 gaggagCCTTCTGTTCATTTCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                                                                                                                 <  2:3936396/150‑1 (MQ=255)
 gaggagCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAATT‑GGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                                                                                                                 <  2:157139/150‑1 (MQ=255)
 gaggagCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                                                                                                                 >  1:264514/1‑150 (MQ=255)
 gaggagCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                                                                                                                                 >  2:5450439/1‑150 (MQ=255)
   ggagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCaaa                                                                                                                                               <  2:1144267/150‑1 (MQ=255)
   ggagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAACACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCaaa                                                                                                                                               <  1:3269643/150‑1 (MQ=255)
   ggacctttCTGTTCTTTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGATCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCaaa                                                                                                                                               <  1:5563500/144‑1 (MQ=255)
    gagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAg                                                                                                                                              >  2:2900246/1‑150 (MQ=255)
        cTTCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                                                                                                                                          <  2:4321041/150‑3 (MQ=255)
         ttCTGTCCAGTCCTTTTTGATTCATTTGGCAGAGC‑CAATATCGCAGCCGCCGCCTCCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCCCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                                                                                                                                         <  2:2147700/150‑1 (MQ=255)
          tCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTtttt                                                                                                                                        <  2:5014491/150‑1 (MQ=255)
                ctttCCTTTTTGATTCATTTGGAAGAGCACAAAAT‑GGAACCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatg                                                                                                                                  <  1:924052/148‑1 (MQ=255)
                cATTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatg                                                                                                                                  >  2:5313057/1‑150 (MQ=255)
                 aTTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatga                                                                                                                                 <  2:1939519/150‑1 (MQ=255)
                 aTTCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCC‑TTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatgat                                                                                                                                <  2:188176/150‑1 (MQ=255)
                  ttCTTTTTTGATTTATTTGATAGAGCAAAAATT‑GTATCTGTATGCTTTCGAATTGTTTTCTAGAGGAATTCTGTGCAATTGGCTTGTGTGACTCGTTTTTTTCATTACAATGTCCCTCAAATGGGAAGGAGCAAAGCTTTTTACCattat                                                                                                                                <  1:5188889/150‑1 (MQ=255)
                  ttCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatgat                                                                                                                                >  1:2038227/1‑150 (MQ=255)
                   tCCTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatgatg                                                                                                                               <  2:4135216/150‑1 (MQ=255)
                     cTTTTTGATTCTTTTGCCAGAGCACAAAAT‑GGATCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCt                                                                                                                             <  1:3579649/150‑1 (MQ=255)
                     cTTTTTGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGGTGCt                                                                                                                             >  2:5514094/1‑150 (MQ=255)
                      tttttGATTCATTTGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCtt                                                                                                                            >  1:4416653/1‑150 (MQ=255)
                           gATTCATTTGGCAGAGCACAAAAT‑GGATCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGt                                                                                                                       <  1:5765442/150‑1 (MQ=255)
                               tatttGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTc                                                                                                                   <  2:3125769/146‑1 (MQ=255)
                               ctttgcgCAGAGCACAATTT‑GCATCTGCCGTCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTTGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTc                                                                                                                   <  2:2862914/144‑1 (MQ=255)
                               cATTTGGCAGAACAAAAAAT‑GGATCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTc                                                                                                                   <  1:4917315/150‑1 (MQ=255)
                                atttGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCt                                                                                                                  >  2:1231984/4‑150 (MQ=255)
                                   tGGCAGAGCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGct                                                                                                               <  1:980381/150‑1 (MQ=255)
                                     gCAGAGCACAAAAT‑GGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttc                                                                                                             <  1:1670433/150‑1 (MQ=255)
                                      caaagCACAAAAT‑GGAACCGCGGGCTTTCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTGGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTATTCTGcttct                                                                                                            <  1:227280/147‑1 (MQ=255)
                                      cAGAGCACAAAAT‑GGACCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttct                                                                                                            <  1:1457930/150‑1 (MQ=255)
                                       agagCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttctt                                                                                                           >  2:757354/1‑150 (MQ=255)
                                         agCACAAAAT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttcttta                                                                                                         <  2:1813645/150‑3 (MQ=255)
                                          gCACAAAAT‑TGTGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCTCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt                                                                                                        <  2:2335327/150‑1 (MQ=255)
                                           cacaAAAT‑GGAACCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAtt                                                                                                       <  1:2408633/150‑1 (MQ=255)
                                              aaaaT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTcc                                                                                                    <  1:2786928/150‑1 (MQ=255)
                                              aaaaT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTcc                                                                                                    >  1:5063114/1‑150 (MQ=255)
                                              aaaaT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTcc                                                                                                    >  1:5611850/1‑150 (MQ=255)
                                                 aT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGc                                                                                                 <  1:1751452/150‑1 (MQ=255)
                                                 aT‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGc                                                                                                 <  2:3699601/150‑1 (MQ=255)
                                                  t‑GGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCt                                                                                                <  2:2481235/150‑1 (MQ=255)
                                                    ggAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCtt                                                                                               <  2:163640/150‑1 (MQ=255)
                                                     gAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCg                                                                                                   >  2:5603717/1‑145 (MQ=255)
                                                     gAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCttt                                                                                              >  1:3415130/1‑149 (MQ=255)
                                                     gAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCttt                                                                                              <  2:2713153/150‑2 (MQ=255)
                                                        ccgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGaa                                                                                           >  1:3700897/1‑150 (MQ=255)
                                                         cgccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCtttggac                                                                                          >  1:3921677/1‑145 (MQ=255)
                                                          gccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAaca                                                                                         >  1:5444232/1‑150 (MQ=255)
                                                            cggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGGAcaca                                                                                       >  2:3644328/3‑150 (MQ=255)
                                                             ggcTTCGGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAg                                                                                      <  2:1685462/149‑1 (MQ=255)
                                                                 tCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCAtt                                                                                  <  2:3589798/150‑1 (MQ=255)
                                                                    gAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGcc                                                                               <  2:592483/150‑1 (MQ=255)
                                                                     aattGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCa                                                                              <  2:2184415/147‑1 (MQ=255)
                                                                       ttGTTTTCCAGAGGAATGCTGTGCAATTGTCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACCCAGCATTGCCAat                                                                            <  2:203737/149‑1 (MQ=255)
                                                                         gTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAatat                                                                          >  1:352035/1‑150 (MQ=255)
                                                                           ttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATAtgt                                                                        <  1:1191508/150‑1 (MQ=255)
                                                                           ttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATAtgt                                                                        <  1:626880/150‑1 (MQ=255)
                                                                                agagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGcaca                                                                   >  2:907718/1‑150 (MQ=255)
                                                                                 gagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACAt                                                                  <  2:5167381/150‑1 (MQ=255)
                                                                                 gagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACAt                                                                  <  1:4195865/150‑1 (MQ=255)
                                                                                   ggaaTGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTc                                                                <  2:3838616/147‑1 (MQ=255)
                                                                                   ggaaTGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTc                                                                >  1:4600425/4‑150 (MQ=255)
                                                                                     aaTGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGc                                                              <  2:1074942/149‑1 (MQ=255)
                                                                                      aTGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTATACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGAATTGCCAATATGTGCACATTCGcc                                                             >  1:79291/1‑150 (MQ=255)
                                                                                          tgtgCAATAGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGAAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGTTTTGAACTCAGCATTGCCAATATGTGCACATTCGCCAtct                                                         >  1:2431376/1‑150 (MQ=255)
                                                                                               aaTTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCtt                                                    >  1:4655864/1‑150 (MQ=255)
                                                                                                  tGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTg                                                 >  1:3637389/1‑150 (MQ=255)
                                                                                                   ggCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGa                                                >  2:4110185/1‑150 (MQ=255)
                                                                                                    gCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGTCAATATGTGCACATTCGCCATCTCGCTTTTGAt                                               <  1:3776690/150‑1 (MQ=255)
                                                                                                    gCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGAt                                               >  1:1955654/1‑150 (MQ=255)
                                                                                                     cTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATc                                              <  2:4546389/150‑1 (MQ=255)
                                                                                                      ttGTGTGCCTCGCCTTTTTCATTTCAATGTCTCTCAAAGGGGAAGGAGCAAAGCCTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCCGCTTTGAACCCAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCg                                             <  1:1301772/150‑1 (MQ=255)
                                                                                                      ttGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGAGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTTCTGATTCGCCATCTCGCTTTTGATCg                                             >  1:1821875/1‑150 (MQ=255)
                                                                                                        gtgtgCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                           >  1:1509005/1‑150 (MQ=255)
                                                                                                         tgtgCCTCGCCTTTTACATTGCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATCATGCTTTTCGTTTTCTGCTTCTTTCTTTACGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGc                                          <  2:1284707/150‑1 (MQ=255)
                                                                                                              cTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCt                                     >  2:4613193/1‑150 (MQ=255)
                                                                                                                 gCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGAt                                  <  2:4416653/150‑1 (MQ=255)
                                                                                                                 gCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGAt                                  >  2:4794987/1‑150 (MQ=255)
                                                                                                                  ccTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATa                                 >  1:3018172/1‑150 (MQ=255)
                                                                                                                    tttttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACa                               >  2:4056570/1‑150 (MQ=255)
                                                                                                                    tttttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACa                               >  1:5223857/1‑150 (MQ=255)
                                                                                                                      tttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGt                             <  1:4230390/150‑1 (MQ=255)
                                                                                                                        tCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACaaaga                           >  1:1040603/1‑146 (MQ=255)
                                                                                                                         cATTCCAATGTCTCTCAAAGTGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTATGTTTCTTTATTTCAGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATTTCTCTTTTGATCGAGCAACCTGATACAGTGAc                          >  1:4729961/1‑150 (MQ=255)
                                                                                                                         cATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGAc                          <  1:2162699/150‑1 (MQ=255)
                                                                                                                         cATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGAc                          <  2:3017880/150‑1 (MQ=255)
                                                                                                                          aTTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                         >  1:4023329/1‑150 (MQ=255)
                                                                                                                              cAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACactga                     <  1:407687/150‑5 (MQ=255)
                                                                                                                              cAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACactga                     >  2:4867200/1‑146 (MQ=255)
                                                                                                                              cAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACactga                     >  2:3303940/1‑146 (MQ=255)
                                                                                                                                 tGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATgg                  <  2:5674562/150‑1 (MQ=255)
                                                                                                                                    ctctCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg               <  1:4466147/150‑1 (MQ=255)
                                                                                                                                         aaaGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTc          <  1:32110/150‑1 (MQ=255)
                                                                                                                                          aaggggaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa         >  2:3426723/1‑150 (MQ=255)
                                                                                                                                          aaggggaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa         >  1:5489779/1‑150 (MQ=255)
                                                                                                                                              ggaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGaaa     <  1:4794987/150‑1 (MQ=255)
                                                                                                                                              ggaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATAGAGCACCCTGATACAGTGACACTGATGGGAGCAGACAGaaa     <  1:2183026/150‑1 (MQ=255)
                                                                                                                                               gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAAc    <  1:1585312/150‑1 (MQ=255)
                                                                                                                                                 aggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGTCACTGATGGGTGCAGTCAGAATCtt  >  2:4678940/1‑150 (MQ=255)
                                                                                                                                                 aggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACtt  >  2:5747546/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAAT‑GGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTT  >  CP006881/3723582‑3723875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑