BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	96.8%	38692.6 / NA	12187	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (23/363); new base T (7147/4587); total (7201/4986)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 12366 total aligned reads displayed.

TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4900‑5198

    gagaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAACCCCCCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                                   <  1:3430558/150‑1 (MQ=255)

     agaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGGGCGCAGGTACCAAATACTGTtcttct                                                                                                                                                  >  2:1751959/1‑150 (MQ=255)

     agaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGGTACCAAATACTGTtcttct                                                                                                                                                  >  1:2642915/1‑150 (MQ=255)

          cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt                                                                                                                                             <  2:2111381/150‑1 (MQ=255)

           tttttttCTGCGCGTAACCTGCTGCTTCCAAACAAAAAACCCCCCGCTACCAGCGGTGGTTTGTTTGCCGGACCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa                                                                                                                                            <  1:3851162/150‑1 (MQ=255)

             tttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGc                                                                                                                                          >  1:1214688/1‑150 (MQ=255)

              ttttCTGCGCGTAATCTGCTGCTTGCACACAAAACAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                                         <  2:3749487/150‑1 (MQ=255)

              ttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAAAACTGTTCTTCTAGTGTAGcc                                                                                                                                         >  1:3568464/1‑150 (MQ=255)

                ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAACACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTATTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGt                                                                                                                                       >  1:1000901/1‑150 (MQ=255)

                    gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCCGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGGAGCCCTAggt                                                                                                                                   >  2:3626836/1‑148 (MQ=255)

                     cgcgTAATCTGCTGCTTGCAAACAAAAAAACCTCCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGACCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTa                                                                                                                                  <  1:3362573/150‑1 (MQ=255)

                     cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCTGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTa                                                                                                                                  >  2:1661070/1‑150 (MQ=255)

                     cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTa                                                                                                                                  >  2:2562315/1‑150 (MQ=255)

                       cgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAGGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGCGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                                >  1:1966548/1‑150 (MQ=255)

                       cgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGAACAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                                <  1:2709236/150‑1 (MQ=255)

                        gTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGGCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc                                                                                                                               >  1:504692/1‑150 (MQ=255)

                        gTAACCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc                                                                                                                               <  2:570056/150‑1 (MQ=255)

                            tCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTGGTGTAGCCGTAGTTAGGccacc                                                                                                                           >  1:845748/1‑150 (MQ=255)

                              tgctgcTTGCAAACACAAAACCCACCGCTACCAGCGGTGGTTTGTTTGCCGGACCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCcaccac                                                                                                                         <  2:2263128/150‑1 (MQ=255)

                                    ttGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACCCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATAACAAATACTGTTCTTCTAGTGTAGCCGGAGTTAGGCCACCACCTCatg                                                                                                                   >  1:2840956/1‑148 (MQ=255)

                                    ttGCAAACAAAAAAACCACCCCAACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                                   <  1:1286146/150‑1 (MQ=255)

                                       caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAc                                                                                                                >  1:2448849/1‑150 (MQ=255)

                                          acaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAActct                                                                                                             >  2:2933320/1‑150 (MQ=255)

                                          acaaaAAAACCACCGCTACCAGCGGTGGTGTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGGActct                                                                                                             >  1:2906428/1‑150 (MQ=255)

                                           caaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTg                                                                                                            >  2:397231/1‑150 (MQ=255)

                                                 aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                                      >  2:3481278/1‑150 (MQ=255)

                                                  tcctctgCTACCAGGGGGGGTTTGTTTGCCGGACCAAGAGCTCCCAACTGTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCACATACTGTCCTGCTAGTGTAGCCGTAGTTAGGCCACCAGTGCAAGAACTCTGTAGCAcc                                                                                                     <  2:3068173/144‑1 (MQ=255)

                                                     accGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGGTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGGAGCACCGcc                                                                                                  >  1:3040648/1‑150 (MQ=255)

                                                      ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCt                                                                                                 >  1:2031140/1‑150 (MQ=255)

                                                      ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCt                                                                                                 <  2:3551059/150‑1 (MQ=255)

                                                           aCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATa                                                                                            >  2:2337771/1‑150 (MQ=255)

                                                           aCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATa                                                                                            >  2:2041322/1‑150 (MQ=255)

                                                            ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAc                                                                                           >  2:1897287/1‑150 (MQ=255)

                                                            ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGGAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAc                                                                                           >  1:2975812/1‑150 (MQ=255)

                                                             gaGCGGTGGTTGGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCACCAGAGCCCAGATACCAAATACTGTTCTTCTAGTGTTGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                                          <  1:1773803/149‑1 (MQ=255)

                                                             cggCGGTGGTTTGTTTGCCGGACCAAGACCTACCAACTCTTTTTCCGAAGGTAACTGGCTCCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                                          <  2:3419185/148‑1 (MQ=255)

                                                                 ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                                      >  2:2793684/1‑150 (MQ=255)

                                                                     gtttgtttGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCCCGCTCTg                                                                                  >  1:1150863/1‑150 (MQ=255)

                                                                     gtttgtttGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGCAACTGGCTTCAGCAGAGCGCAGATACCAAGTACTGTTCTTCTAGTGTAGCCGCAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCCCTCTg                                                                                  >  1:3502856/1‑150 (MQ=255)

                                                                           ttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATc                                                                            >  1:775903/1‑150 (MQ=255)

                                                                            tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGGTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAAACCTCGCCCTGCTAATcc                                                                           >  1:3629336/1‑150 (MQ=255)

                                                                                 gaccAAGAGCTACCAACTCTTTTTCCGAGGGTAACTGGCCTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                                      <  2:478501/147‑1 (MQ=255)

                                                                                 gATCAAGAGCTACCAATTTTTTTTCGGAAGGTACCTGGCTTCAGCAGAGCGCAGATCCCAACTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                                      <  2:675921/150‑1 (MQ=255)

                                                                                 gATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                                      >  2:2489150/1‑150 (MQ=255)

                                                                                   tCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAcc                                                                    >  1:3236627/1‑150 (MQ=255)

                                                                                    cAAGAGCTCCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCa                                                                   <  1:1700762/150‑1 (MQ=255)

                                                                                    cAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAACCCTGTTACCa                                                                   >  1:3107304/1‑150 (MQ=255)

                                                                                    cAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGGTAGGCCACCACTTCAAGAACTCTGTAGCACCCCCTACATACCTCGCTCTGCTAATCCTGTTACCa                                                                   >  1:3928192/1‑150 (MQ=255)

                                                                                       gagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGGGCAGATACCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTg                                                                <  1:1074536/150‑1 (MQ=255)

                                                                                       gagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTg                                                                <  2:270892/150‑1 (MQ=255)

                                                                                       gagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGGGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTTCTAATCCCGTTAcccggg                                                                >  1:717334/1‑146 (MQ=255)

                                                                                         gCTACCACCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgc                                                              <  2:3147115/150‑1 (MQ=255)

                                                                                         gCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgc                                                              >  2:763444/1‑150 (MQ=255)

                                                                                         gCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgc                                                              >  2:1423178/1‑150 (MQ=255)

                                                                                          cTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGGTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAACTCGCTCTGCTAATCCTGTTACCAGTGgct                                                             >  2:1568387/1‑150 (MQ=255)

                                                                                            cccAACTCTTTTCCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctgc                                                           <  1:2573536/149‑1 (MQ=255)

                                                                                            aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctgc                                                           <  1:2165852/150‑1 (MQ=255)

                                                                                              cacccctTTTTCCGAAGGTAACTGGCTCCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGctgctg                                                         <  2:1124967/145‑1 (MQ=255)

                                                                                               aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGGTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGGGGCtgctgc                                                        >  1:2102270/1‑150 (MQ=255)

                                                                                               aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCtgctgc                                                        >  2:3287708/1‑150 (MQ=255)

                                                                                               aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCtgctgc                                                        >  1:1425390/1‑150 (MQ=255)

                                                                                                aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTTATCCTGTTACCAGTGGCTGCTGcc                                                       >  1:1352935/1‑150 (MQ=255)

                                                                                                aCTCTTTTTCCGAAGGTAACTGGCTCCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       <  1:3699602/150‑1 (MQ=255)

                                                                                                 ctctTTTTCCGATGGTAACTGGCTTCAGCCGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGGAGCACCGCCTACAGACCCCGCTCTGCTAATCCTGTTACCAGTGGCtgctgcga                                                      >  1:3774416/1‑148 (MQ=255)

                                                                                                 ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  2:1825702/1‑150 (MQ=255)

                                                                                                    tttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCACATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCGGTg                                                   >  1:920782/1‑150 (MQ=255)

                                                                                                    tttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGGGGCTGCTGCCAGTg                                                   >  1:2225964/1‑150 (MQ=255)

                                                                                                     ttttCCGAAGGTAACTGGCTTCACCAGAGCGCAGATCCCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTgg                                                  <  2:3215007/150‑1 (MQ=255)

                                                                                                     ttctCCGAAGGTATCTGGCTACAGCAGAGCGCAGCTACCATATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTgg                                                  <  1:3163080/147‑1 (MQ=255)

                                                                                                      tttCCGAAGGTAACTGGCTTCACACGAGCGCAGCTACCATATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTCCAAGAACTCTGTAGCACCGCCTCCATCCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGc                                                 <  2:3348555/150‑1 (MQ=255)

                                                                                                        tCCGAGGGTAACTGGCTCCAGCAGAGCGCAGATACCACATCCTGTTCTCCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                               <  1:979764/150‑1 (MQ=255)

                                                                                                        tCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                               <  1:1839319/150‑1 (MQ=255)

                                                                                                          cGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGCGGCCATa                                             >  1:1560434/1‑150 (MQ=255)

                                                                                                           gAAGGTACCTGGCTCCACCCGAGCCGAGTTCCCAATTCCTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGTACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATaa                                            <  1:2300914/150‑1 (MQ=255)

                                                                                                           gAAGGTAACTGGCTTCAGCAGGGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATaa                                            >  1:566932/1‑150 (MQ=255)

                                                                                                            aaGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAg                                           <  2:2637401/150‑1 (MQ=255)

                                                                                                               gtaccgggCTTCAGCAGAGCGCAGTTACCAAATACTTTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCg                                        <  2:2864617/144‑1 (MQ=255)

                                                                                                               gTAACTGGCTCCGGCAGAGCGCAGATCCCAAATACTGTTCTCCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCg                                        <  1:2515361/150‑1 (MQ=255)

                                                                                                                  aCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTCCTAGGGTAGCCGAGGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtgt                                     <  1:1633604/150‑1 (MQ=255)

                                                                                                                    tGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGGGATAAGTCGTGTCt                                   >  1:421303/1‑150 (MQ=255)

                                                                                                                    tGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCt                                   >  2:2405972/1‑150 (MQ=255)

                                                                                                                     ggCTCCAGCAGAGCGCAGATACCAATTACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCtt                                  <  2:3005346/150‑1 (MQ=255)

                                                                                                                     gcccccagcagAGCCAAGTAACCAAATACTGTTCTTCTAGTGTAGCGGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCtt                                  <  1:1499080/145‑1 (MQ=255)

                                                                                                                      gCTTCAGCAGGGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTa                                 >  1:644942/1‑150 (MQ=255)

                                                                                                                      gCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGGTAAGTCGTGTCTTa                                 >  1:1914042/1‑150 (MQ=255)

                                                                                                                      gCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTa                                 >  2:917607/1‑150 (MQ=255)

                                                                                                                          cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCgg                             >  2:1201446/1‑150 (MQ=255)

                                                                                                                           agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCggg                            <  2:3819235/150‑1 (MQ=255)

                                                                                                                             cagAGCGCAGATCCCAACTATTTTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGtt                          <  2:842340/150‑1 (MQ=255)

                                                                                                                                    cAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTCCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCa                   <  2:2191384/150‑1 (MQ=255)

                                                                                                                                    cAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCa                   >  2:1968681/1‑150 (MQ=255)

                                                                                                                                       aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGa                <  2:1275102/150‑1 (MQ=255)

                                                                                                                                        tcccAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc               <  2:1499196/148‑1 (MQ=255)

                                                                                                                                        tACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc               >  1:3303142/1‑150 (MQ=255)

                                                                                                                                        tACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCCCCTACATACCTCGCTCTGCTAATCCTGATACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc               >  1:2371056/1‑150 (MQ=255)

                                                                                                                                         aCCACATACTGTTCTTCTAGTGTAGCCGTAGTTGGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACg              <  2:1344814/150‑1 (MQ=255)

                                                                                                                                             aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAg          <  2:3890143/150‑1 (MQ=255)

                                                                                                                                               tACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGGGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGGTAGtt        >  1:2772744/1‑150 (MQ=255)

                                                                                                                                                aCTGTTCTTCTAGTGTAGCCGTAGTTCGCCCACCACTCCAAGACCTCTGTAGCACCGCCTACATACCCCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa       <  2:1046680/150‑1 (MQ=255)

                                                                                                                                                    tcctccTAGGGTAGCCGTCGTTAGGCCACCACTCCAAGACCCCTGTGGCCCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCgg   <  2:3690460/150‑1 (MQ=255)

TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4900‑5198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`