Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,344 C→T S53F (TCT→TTT)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,3440CT99.7% 1245.2 / ‑5.7 330S53F (TCT→TTT) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (192/137);  minor base A (1/0);  total (193/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 335 total aligned reads displayed.

AAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGC  >  CP006881/3723197‑3723490
                                                                                                                                                   |                                                                                                                                                  
aaGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGGTATTTCTTTa                                                                                                                                                  >  2:1024806/1‑150 (MQ=255)
 aGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTAt                                                                                                                                                 <  1:3728674/150‑1 (MQ=255)
  gCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATATCTTTATg                                                                                                                                                >  1:660178/1‑150 (MQ=255)
      tacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGctg                                                                                                                                            >  2:845955/3‑148 (MQ=255)
        cAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGctggt                                                                                                                                          >  2:3356123/1‑146 (MQ=255)
            aggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTCGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCCGCTATTCCTATATGGCTGTTtctc                                                                                                                                      >  2:3962166/3‑150 (MQ=255)
                   aCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATACTTATTGGTTTTGGCATTACAGCCGCAAGCAACACAGGTGGCTATTTCTTTATGGCTGATTCTCCGTTTGc                                                                                                                               >  2:1176746/1‑150 (MQ=255)
                     aGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGcct                                                                                                                             >  2:1315951/1‑148 (MQ=255)
                      gTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAATTCTTTATGGCTGATTCTCCGTTTGCctt                                                                                                                            >  2:2819046/1‑150 (MQ=255)
                         tgCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTcc                                                                                                                         >  2:3675131/2‑150 (MQ=255)
                         tgCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTcc                                                                                                                         <  1:294622/149‑1 (MQ=255)
                          gCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCg                                                                                                                        >  1:3023445/1‑150 (MQ=255)
                              tGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAg                                                                                                                    >  1:535656/1‑150 (MQ=255)
                               gAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGc                                                                                                                   >  2:715754/1‑150 (MQ=255)
                               gAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGc                                                                                                                   >  2:309638/1‑150 (MQ=255)
                                   aaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGcc                                                                                                                  >  1:3061026/1‑147 (MQ=255)
                                   aaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCt                                                                                                               >  2:548408/1‑150 (MQ=255)
                                      caaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTggc                                                                                                            >  2:1441828/3‑148 (MQ=255)
                                       aaaaCATATTCGCTTCAAGCAAATTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTca                                                                                                           <  1:1319909/149‑1 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGGTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcact                                                                                                         >  2:3595520/1‑149 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcact                                                                                                         >  2:1681698/1‑149 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcact                                                                                                         >  2:2215027/1‑149 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcact                                                                                                         >  1:2444373/1‑149 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcacc                                                                                                         >  2:2765718/1‑149 (MQ=255)
                                          aCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcactt                                                                                                        <  1:1495793/150‑3 (MQ=255)
                                              attcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                    >  2:1131157/7‑150 (MQ=255)
                                              attcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                                    >  2:766359/7‑150 (MQ=255)
                                                   cttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                               <  1:1360162/149‑1 (MQ=255)
                                                   cttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                               <  1:3820639/149‑1 (MQ=255)
                                                       aaGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAt                                                                                           >  2:2129755/2‑150 (MQ=255)
                                                          cAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGGCATTct                                                                                        >  1:1002707/1‑150 (MQ=255)
                                                          cAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTct                                                                                        >  2:2066961/1‑150 (MQ=255)
                                                           aaaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCAGATTCTCCGTTTGCCTCTCCGGCAGCCGCCGTCACTTTCGGGGCCGCCAGTctg                                                                                       >  2:3064639/1‑150 (MQ=255)
                                                           aaaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCACCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCCCCGTCACTTTCGGGGCCGCCATTccg                                                                                       >  1:2110235/1‑148 (MQ=255)
                                                             aaTCGGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCACCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGGCGCTGTCACTTTCGGGGCCGCTATTctgat                                                                                     <  2:273756/150‑3 (MQ=255)
                                                             aaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCCCTTTCGGGGCCGCTATTctgat                                                                                     >  1:1279913/1‑148 (MQ=255)
                                                               tCCGTTATGTGGTTCGGTCCATTTTGGCCAGTATATTTATTGGTTGTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATATCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCtgatga                                                                                   <  1:2157473/150‑1 (MQ=255)
                                                               tCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCtgatga                                                                                   >  1:3160176/1‑150 (MQ=255)
                                                                 cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTGTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGtt                                                                                 >  2:3934948/1‑150 (MQ=255)
                                                                  gTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTg                                                                                >  2:3557672/1‑150 (MQ=255)
                                                                         gCTCCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACgg                                                                         <  1:482305/150‑1 (MQ=255)
                                                                           ttcggtCCTTTTTGGCCATTTTATTTATTGTTTTTGGCATTTCAGCCGCAAGCAAAACACGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAg                                                                       <  1:1058169/145‑1 (MQ=255)
                                                                           ttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAg                                                                       >  2:2675278/6‑150 (MQ=255)
                                                                           ttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAg                                                                       >  2:319604/6‑150 (MQ=255)
                                                                             cggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGc                                                                     >  2:2025997/4‑150 (MQ=255)
                                                                              ggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCg                                                                    <  2:108640/148‑1 (MQ=255)
                                                                               gtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGAGGCgg                                                                   >  2:1738674/2‑150 (MQ=255)
                                                                               gtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCgg                                                                   >  2:2895261/2‑150 (MQ=255)
                                                                               gtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCCGCCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGGGGCgg                                                                   >  2:2185451/2‑150 (MQ=255)
                                                                                 gcATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGgag                                                                 <  1:1651301/149‑1 (MQ=255)
                                                                                 ccATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCTGGGCCGCCATTCTGATGATTGCTTACGGAGGCGgag                                                                 >  2:889526/1‑150 (MQ=255)
                                                                                 ccATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGgag                                                                 >  2:1237382/1‑150 (MQ=255)
                                                                                  cATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGggga                                                                >  2:3816452/1‑150 (MQ=255)
                                                                                  cATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGgaga                                                                <  1:2306527/150‑1 (MQ=255)
                                                                                   aTTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGat                                                               <  1:1859339/150‑1 (MQ=255)
                                                                                    ttttGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAACCAGGCAGCTATTTCTTTATGGCTGATTCCCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatt                                                              <  1:407649/150‑1 (MQ=255)
                                                                                      ttGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGattta                                                            <  2:3766134/150‑1 (MQ=255)
                                                                                      ttGGCCAGTATATTTATTGGTTTTGCCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGattta                                                            <  1:357523/150‑1 (MQ=255)
                                                                                       tGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGGGatttat                                                           >  2:60259/1‑150 (MQ=255)
                                                                                        ggCCAGTATATTTATTGGTTTGGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTCCTTTATGGCTGATCCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatttatt                                                          <  2:2796395/150‑1 (MQ=255)
                                                                                         gCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatttattt                                                         >  1:2594247/1‑150 (MQ=255)
                                                                                          ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAACCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGAtttattta                                                        <  2:3214959/150‑1 (MQ=255)
                                                                                          ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGAtttattta                                                        >  2:532097/1‑150 (MQ=255)
                                                                                             gtatatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGGGATTTATTTAccg                                                     >  1:2086668/6‑148 (MQ=255)
                                                                                             gtatatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCCGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTAccg                                                     >  1:107293/6‑148 (MQ=255)
                                                                                               atatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGAGGTTTAGTTACCGGc                                                   >  2:488136/4‑150 (MQ=255)
                                                                                                 ctttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                                 <  1:1122507/149‑1 (MQ=255)
                                                                                                   ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAaca                                               >  2:3509506/1‑150 (MQ=255)
                                                                                                    tATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGGGATTTATTTACCGGCAacac                                              >  2:151819/1‑150 (MQ=255)
                                                                                                    tATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAacac                                              <  1:2853934/150‑1 (MQ=255)
                                                                                                         gttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGGGGCGGGGATTTATTTACCGGCAACACctttt                                         >  2:3647521/6‑150 (MQ=255)
                                                                                                          ttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCATTTa                                        <  1:809074/146‑1 (MQ=255)
                                                                                                                cATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAc                                  <  1:3199840/150‑1 (MQ=255)
                                                                                                                  ttACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAcct                                <  1:365487/150‑3 (MQ=255)
                                                                                                                  tcaCAGCCGCAAGCAAAACAGGCAGCTGTTTCTTTATGGCTGATTCTCCGTTTGCCCTTCCGGCAGCCGCTGTCACTCTCGGGGCCGCTATGCTGTTGATTGCTCACGGGGGCGGAGATTTATTTACCGGCAACACGTTTTATTTCAcct                                <  1:701061/148‑3 (MQ=255)
                                                                                                                   tACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTGACCta                               <  1:3430022/150‑1 (MQ=255)
                                                                                                                    aCAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCtat                              >  2:1495058/1‑150 (MQ=255)
                                                                                                                      aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCCCTATTCTGATGGTTGCTCACGGGGGCGGGGATTTATTTACCGGCAGCACCTTTCAATTCACCTATAc                            >  2:1815098/1‑150 (MQ=255)
                                                                                                                       gCCGCAAGCAAACCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAcc                           <  2:3453800/150‑1 (MQ=255)
                                                                                                                       gCCGCAACCTACACCGGCAGCTGTCTCTTTGTGGCTGATCCTCCGTTAGCCTTTCCGCCAGCCGCTGTCTCTTTCGGGGCCGCTATTCTGATGATTCCCTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAcc                           <  2:3037572/150‑1 (MQ=255)
                                                                                                                        ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACcg                          <  2:3125584/150‑1 (MQ=255)
                                                                                                                          ggaagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGATGATTGCTTACGGGGGCGGGGATTTAGTTACCGGCAACACCTTTTAATTCACCTATACcgcg                        >  2:2328079/3‑150 (MQ=255)
                                                                                                                           caagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGGGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCgcgc                       >  1:3244394/1‑150 (MQ=255)
                                                                                                                           caagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCgcgc                       >  2:425656/1‑150 (MQ=255)
                                                                                                                            aagcaaAACAGGCAGCTATATCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCt                      >  2:1931374/1‑150 (MQ=255)
                                                                                                                                 aaaCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCgga                 <  1:2048391/150‑3 (MQ=255)
                                                                                                                                 aaaCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCgga                 <  1:3557672/150‑3 (MQ=255)
                                                                                                                                  aaCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTccagaa                <  1:1263499/150‑5 (MQ=255)
                                                                                                                                    cAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGAGGTTTATTTACCCGCAACACCTTTTATTTCACCTATACCGCGCTCCCgaaaa              >  1:980597/1‑150 (MQ=255)
                                                                                                                                    cAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCACCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaa              <  1:1992364/150‑1 (MQ=255)
                                                                                                                                      ggCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaaaa            >  2:2454218/1‑150 (MQ=255)
                                                                                                                                      ggCAGCTATTTCTTTATGGCTGACTCTCCGTTTGCCTTACCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaaaa            <  1:2220974/150‑1 (MQ=255)
                                                                                                                                         agctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtc         <  1:2693779/147‑2 (MQ=255)
                                                                                                                                         agctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtc         <  1:1780553/147‑2 (MQ=255)
                                                                                                                                           ctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGAGGTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcag       >  2:520437/2‑147 (MQ=255)
                                                                                                                                           ctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcag       <  1:2194145/149‑4 (MQ=255)
                                                                                                                                            tATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcagc      >  2:692289/1‑146 (MQ=255)
                                                                                                                                             aTTCCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcagct     <  2:1279913/150‑6 (MQ=255)
                                                                                                                                               ttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTgg   <  1:2766003/150‑1 (MQ=255)
                                                                                                                                                tcttTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTCCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGc  <  1:2543212/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGC  >  CP006881/3723197‑3723490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑