Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,542 A→G S95S (TCT→TCC)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,5420AG99.1% 812.3 / NA 216S95S (TCT→TCC) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (126/87);  minor base C (2/0);  total (128/88)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

GACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAA  >  CP006881/3724396‑3724688
                                                                                                                                                  |                                                                                                                                                  
   acGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCACTTTGAATCCTTTCGATCGCAAAGGGCAAATGGTCGGAAAgc                                                                                                                                              >  1:2191100/1‑150 (MQ=255)
    cGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGGTCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                                             >  1:1663297/1‑150 (MQ=255)
     gCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                                            >  2:268371/1‑150 (MQ=255)
      cccAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGcgcg                                                                                                                                           <  2:3545002/150‑1 (MQ=255)
       ccATTGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAGGGACAAATGCTCGGAAAGCGCGa                                                                                                                                          >  1:3346846/1‑150 (MQ=255)
       ccAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTTTCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGa                                                                                                                                          >  1:3223147/1‑150 (MQ=255)
         aaTGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCCCCTATATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGCTCGCAAAGGACAAATGGTCGGGAAGCGCGata                                                                                                                                        >  1:3880741/1‑150 (MQ=255)
            gTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCTGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGa                                                                                                                                     >  1:1474031/1‑150 (MQ=255)
              ctctTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                                                                                                                   <  2:3053525/150‑1 (MQ=255)
               tctTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtg                                                                                                                                  >  1:3316934/1‑150 (MQ=255)
                   ttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAt                                                                                                                              <  2:461627/150‑1 (MQ=255)
                   ttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGAC‑AATGGTCGGAAAGCGCGATATGAATGTGATc                                                                                                                             <  2:2866234/150‑1 (MQ=255)
                    tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATc                                                                                                                             >  1:3750988/1‑150 (MQ=255)
                     aCATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAACTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGAAATGAATGTGGTCg                                                                                                                            >  1:1374665/1‑150 (MQ=255)
                       aTGTCCGATCGCCCACCGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCt                                                                                                                          <  1:3471144/150‑1 (MQ=255)
                         gTCCGATCGCCCACAGGCCGTTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCCGAAAGCGCGATATCAATGGGATCGCTCa                                                                                                                        >  1:2685901/1‑150 (MQ=255)
                         gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCa                                                                                                                        <  1:2028160/150‑1 (MQ=255)
                         gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCa                                                                                                                        <  2:3082994/150‑1 (MQ=255)
                            cGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAgcg                                                                                                                     <  2:793622/150‑1 (MQ=255)
                               tCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAgcggcg                                                                                                                  >  1:294153/1‑150 (MQ=255)
                                cGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGAGAGCGCTCAGCGgcgc                                                                                                                 >  1:2123472/1‑150 (MQ=255)
                                 gCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgcc                                                                                                                >  1:3746124/1‑150 (MQ=255)
                                 gCCCACAGGCCGATCCCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgcc                                                                                                                <  2:916791/150‑1 (MQ=255)
                                    cacaGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                                                                                                             <  2:1810748/150‑1 (MQ=255)
                                      caGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcggg                                                                                                           >  1:196436/1‑148 (MQ=255)
                                      caGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAg                                                                                                           >  1:1044908/1‑150 (MQ=255)
                                       aGGCCGTTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCCATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGCTCGCAAAGGACAAATGGTCGGAGAGCGCGATATGAATGTGTCTGCTCAGCGGGGCCGCGAGc                                                                                                          >  1:733474/1‑150 (MQ=255)
                                       aGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATATATTCTACACAAGCAGCCCATTTTGAATCCTTTCGATCACAAACGAAAAATGGTCGCAAAGGTCGTTATGAAAGTGAACGCCCACAGGcgccacaggc                                                                                                          >  1:640282/1‑144 (MQ=255)
                                           cGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGGTCGCAAAGGACAAATGGTCGGAAAGCGCGGTATGAATGTGATCGCTCAGCGGCGCCGCGGGCTctc                                                                                                      >  2:3222840/1‑148 (MQ=255)
                                           cGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctc                                                                                                      <  1:2994699/150‑3 (MQ=255)
                                           cGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctc                                                                                                      <  1:343425/150‑3 (MQ=255)
                                           cGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctc                                                                                                      >  1:314161/1‑148 (MQ=255)
                                            gATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTctcc                                                                                                     >  2:66075/1‑150 (MQ=255)
                                              tctcATATTCTTTTTTAAAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCtt                                                                                                   <  2:1072241/150‑1 (MQ=255)
                                                 catATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAGTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCTGCGGCGCCGCGAGCTCTCCTTCCg                                                                                                >  1:919505/3‑150 (MQ=255)
                                                 catATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGTTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg                                                                                                >  1:3550623/3‑150 (MQ=255)
                                                 catATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCCTCCg                                                                                                >  1:336955/3‑150 (MQ=255)
                                                  atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGc                                                                                               >  1:429291/2‑150 (MQ=255)
                                                   tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                              >  2:957460/1‑150 (MQ=255)
                                                   tATCCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                                              <  2:3951181/150‑1 (MQ=255)
                                                    tttCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTTTTCTGCACAAGCAGCCCATTTTGACTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt                                                                                             <  2:2177734/149‑1 (MQ=255)
                                                      tCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGGGCCGCGAGCTCTCCTTCCGCATAg                                                                                           >  1:2583939/1‑150 (MQ=255)
                                                      tCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAg                                                                                           >  1:1606542/1‑150 (MQ=255)
                                                       cTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                                          <  2:1784251/150‑1 (MQ=255)
                                                           tttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgat                                                                                      >  1:2848330/1‑150 (MQ=255)
                                                              ataGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCTTCCGCATAGCTggtgat                                                                                   >  2:891307/1‑150 (MQ=255)
                                                              ataGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                                                   <  1:1112136/150‑1 (MQ=255)
                                                              ataGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                                                   >  2:3752096/1‑150 (MQ=255)
                                                                 gagGGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATc                                                                                <  2:315489/150‑1 (MQ=255)
                                                                 gagCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCCCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATc                                                                                <  2:1528025/150‑1 (MQ=255)
                                                                  agCGCCTTTATCCCATCCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTGGATCGCAAAGGACAAATGGCCGGAAAGCGCGATATGAATGTGATCGCCCAGCGGCGCCGGGAGCTCTCCTTCCGCATAGCTGATGATATcc                                                                               <  2:3880741/150‑1 (MQ=255)
                                                                    cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGGAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTc                                                                             >  1:323486/1‑150 (MQ=255)
                                                                     gcctttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCa                                                                            <  2:2650040/147‑1 (MQ=255)
                                                                        tttATCCCATGCAGCAATTTATCCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCCCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCt                                                                         <  2:3173774/150‑1 (MQ=255)
                                                                         ttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                                                        <  2:3936609/150‑1 (MQ=255)
                                                                          tATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTAt                                                                       >  2:350533/1‑150 (MQ=255)
                                                                               cATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATTTGAATGTGTTCGCTCAGCGGCGCCGCCGGCTCTCCTTCCGCATAGCTGATGATATCCCCAGCTATTTctc                                                                  >  1:1840756/1‑148 (MQ=255)
                                                                                 tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGGTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCCTCCGCATAGCTGATGGTATCCTCACCTATTTctcta                                                                >  1:561690/1‑146 (MQ=255)
                                                                                    agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGGTATCCTCAGCTATTTCTATATGc                                                             >  2:2586863/1‑150 (MQ=255)
                                                                                     gcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATTGCTGATGATATCCTCAGCTATTTCTATATGct                                                            >  2:246336/1‑150 (MQ=255)
                                                                                      caATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGTTGATATCCTCAGCTATTTCTATATGctc                                                           >  2:1420947/1‑150 (MQ=255)
                                                                                      caATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCCCTCCTTCCGCATAGCTGATGATATCCTCAGCTCTTTCTCTATGctc                                                           >  1:2738813/1‑150 (MQ=255)
                                                                                       aaTTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGGTATCCTCAGCTATTTCTATATGcttc                                                          >  1:2434616/1‑148 (MQ=255)
                                                                                       aaTTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGAGATCCTCACCTTTTTCTATATggttc                                                          >  1:2338813/1‑146 (MQ=255)
                                                                                         tttATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcctc                                                        >  1:818810/1‑150 (MQ=255)
                                                                                         tttATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCTTCCGCATAGCCGATGATATCCTCACCCATTTCTATATGctcctc                                                        >  1:1315348/1‑150 (MQ=255)
                                                                                         tttATTCTGCACAAGCAGCCCATTTTGAATCCTTCCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCTCTCATCGGCGCCGCGAGCACTCCTTCCGCATATCAGATGATATCCTCCGCTATTTCAATATGctcctc                                                        >  1:1341015/1‑150 (MQ=255)
                                                                                            aTTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTACATGCTCCTCCgg                                                     >  2:2446041/1‑150 (MQ=255)
                                                                                            aTTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTAGGAAAGCGCGATATGAATGTGATCGCCCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCgg                                                     <  2:822181/150‑1 (MQ=255)
                                                                                             ttCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                                                    >  1:3636512/1‑150 (MQ=255)
                                                                                              tCTGCACAAGCAGCTCATTTTGAACCCTGTCGTTCGCAAAGGACAATTGGACGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCACCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                                                   <  1:3569721/150‑1 (MQ=255)
                                                                                                tGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTGGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgt                                                 <  2:2793535/150‑1 (MQ=255)
                                                                                                tGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGGAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCACAGCTGATGCTATCCTCAGCTATGTCTATATGCTCCTCCGGCact                                                 >  2:220150/1‑148 (MQ=255)
                                                                                                        cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTAATTCTATATGCTCCTCCGGCACTGGTTGCAg                                         >  2:1306059/1‑150 (MQ=255)
                                                                                                         agcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAgg                                        >  2:1948922/1‑150 (MQ=255)
                                                                                                           cccATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAggtt                                      >  2:1545146/1‑148 (MQ=255)
                                                                                                            ccATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCTCATATCTGATGATATCTTAAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGAtt                                     <  1:997965/150‑1 (MQ=255)
                                                                                                              aTTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCCCGAGCTCTCCTTCCGCATAGCTGGTGATATCCTCCGCTATTTCTATATGCTCCCCCGGCAGTGTTTGCCGGATTTg                                   >  1:3536232/1‑150 (MQ=255)
                                                                                                              aTTTTGAATCCTTCCGATCGCAAAGGCCAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTg                                   <  2:534532/150‑1 (MQ=255)
                                                                                                               ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGACCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGt                                  <  2:2093976/150‑1 (MQ=255)
                                                                                                                tttGAACCCTTTCGACCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtt                                 <  2:3821219/150‑1 (MQ=255)
                                                                                                                   gAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCACCGGGGCCGCGTGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCCGCAGTGTTTGCAGGATTTGtttgc                              >  1:340305/1‑148 (MQ=255)
                                                                                                                       ccTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGGTTGCAGGATTTGTTTGTACtt                          >  1:3263313/1‑150 (MQ=255)
                                                                                                                         tttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCCCCGGCAGTGTTTGCAGGATTTGTTTGAACtttt                        <  1:2414807/150‑1 (MQ=255)
                                                                                                                         ttgcGATCGCAAAGGCCAAGTGGTCGGAAAGCGCGATATGAATGTGATCGCTCACCGGCGCCCCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACtttt                        <  1:17422/147‑1 (MQ=255)
                                                                                                                            cGATCGCAAAGGACAAATGGTCGGAAAGCGCGTTATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATTGCTGATGTTAACCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGGACTTTTCAt                     >  2:2342239/1‑150 (MQ=255)
                                                                                                                            cGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTTAt                     >  1:3792494/1‑150 (MQ=255)
                                                                                                                            cGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCATTATGCTCCCCCCGCAGGGTTTGCGGGATTTGTTTGAACTTTTCAt                     >  1:3119899/1‑150 (MQ=255)
                                                                                                                             gATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGGGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGGTTTGTTTGAACTTTTCAtt                    >  2:2657129/1‑150 (MQ=255)
                                                                                                                               ccGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATtct                  <  2:3352630/149‑1 (MQ=255)
                                                                                                                                cGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGGACTTTTCATtctc                 >  2:1722654/1‑150 (MQ=255)
                                                                                                                                cGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTATTCATtctc                 >  2:2936793/1‑150 (MQ=255)
                                                                                                                                 gCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGGTTGAACTTTTCATTCtcg                >  2:2479752/1‑150 (MQ=255)
                                                                                                                                  cAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGCTGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGGGTTTGCAGGATTTGTTTGGACTTTTCATTCtcgt               >  2:2744153/1‑150 (MQ=255)
                                                                                                                                  cAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgt               <  1:3782830/150‑1 (MQ=255)
                                                                                                                                     aGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATGTGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGTACTTTTCCTTCtcgtcgg            >  2:2009384/1‑149 (MQ=255)
                                                                                                                                       gACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGGTATCCTCAGCTATTTCTAAATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCgcgc          >  1:549737/1‑150 (MQ=255)
                                                                                                                                          aaaTGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGGTTGAACTTTTCATTCTCGTCGCGCACg       >  2:3517098/1‑150 (MQ=255)
                                                                                                                                              ggTCTGAAAGAGCGATATGCTTTTGATCGCTCAGCGGCGCCGCATGCTCTCCTTCCGCATAGCTGATGATTTCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACaa   <  1:1995691/150‑1 (MQ=255)
                                                                                                                                               gTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGGGTTTGCAGGATTTGTTTGGACTTTTCATTCTCGTCGCGCACGACaaa  >  2:360396/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAA  >  CP006881/3724396‑3724688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑