BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,655,978	Δ1 bp	35.5%	coding (430/1596 nt)	U712_08515 →	Flagellar M‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,655,978	0	G	.	35.5%	872.6 / inf	386	coding (430/1596 nt)	U712_08515	Flagellar M‑ring protein
Reads supporting (aligned to +/- strand): ref base G (120/129); new base . (68/69); total (188/198)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 391 total aligned reads displayed.

TGGCTGCAGAAGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATATCATCTG  >  CP006881/1655829‑1656127

tGGCTGCAGAAGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACg                                                                                                                                                       >  2:327300/1‑150 (MQ=255)

   cTGCAGAAGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTa                                                                                                                                                    <  2:1625216/150‑1 (MQ=255)

        gAAGGACTTCCAAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTaaa                                                                                                                                               <  1:4016129/150‑1 (MQ=255)

         aaGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTaaaa                                                                                                                                              <  2:42290/150‑1 (MQ=255)

         aaGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTaaaa                                                                                                                                              >  1:156568/1‑150 (MQ=255)

           ggACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAAtt                                                                                                                                           <  2:2832387/150‑1 (MQ=255)

           ggACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAAtt                                                                                                                                           >  1:5839398/1‑150 (MQ=255)

               ttCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCaa                                                                                                                                        >  2:5726081/1‑150 (MQ=255)

                  cTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAg                                                                                                                                     <  1:2952309/150‑1 (MQ=255)

                   tAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTa                                                                                                                                   <  1:5625866/150‑1 (MQ=255)

                   tAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTa                                                                                                                                   >  1:4679809/1‑150 (MQ=255)

                    aaaaCGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAAGTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTa                                                                                                                                   >  2:5232658/1‑150 (MQ=255)

                    aaaaCGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTa                                                                                                                                   >  1:373069/1‑150 (MQ=255)

                      aaCGGGATCAATTGATTATTCGTTTGTTGGACAGAATGCCGGCTTTGGTTTGTCAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATg                                                                                                                                <  2:1570579/150‑1 (MQ=255)

                       aCGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGa                                                                                                                               <  2:3500099/150‑1 (MQ=255)

                        cGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGAt                                                                                                                              <  2:5334873/150‑1 (MQ=255)

                         gggATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGAtt                                                                                                                             >  2:3858122/1‑150 (MQ=255)

                          ggATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGAtt                                                                                                                             <  1:4339063/150‑1 (MQ=255)

                           gATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTa                                                                                                                            <  2:3147371/150‑1 (MQ=255)

                             tCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAAtc                                                                                                                         >  1:2795038/1‑150 (MQ=255)

                              cAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAAtct                                                                                                                        >  1:1114209/1‑150 (MQ=255)

                              cAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAAtct                                                                                                                        >  2:578713/1‑150 (MQ=255)

                               acttGATTATTCGTTTTTTGCACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAAtct                                                                                                                        <  1:940692/148‑1 (MQ=255)

                                    attattCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGa                                                                                                                   >  2:4091706/1‑150 (MQ=255)

                                     ttattCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGACTAATCTCCCGaaa                                                                                                                 <  2:2435434/150‑1 (MQ=255)

                                      tattCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGaaa                                                                                                                 >  2:2188815/1‑150 (MQ=255)

                                       attCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGa                                                                                                               >  2:3784262/1‑150 (MQ=255)

                                        ttCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGa                                                                                                               >  1:1542802/1‑150 (MQ=255)

                                        ttCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGa                                                                                                               >  2:3453335/1‑150 (MQ=255)

                                         tCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGAc                                                                                                              >  2:1289723/1‑150 (MQ=255)

                                         tCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGAc                                                                                                              <  1:5529386/150‑1 (MQ=255)

                                           gTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAGACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGc                                                                                                            >  2:4312584/1‑150 (MQ=255)

                                              ttttGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCtgt                                                                                                         <  1:121412/150‑1 (MQ=255)

                                                 tGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGtt                                                                                                      <  2:3728809/150‑1 (MQ=255)

                                                     cAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTc                                                                                                  >  1:2146652/1‑150 (MQ=255)

                                                       gAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCgg                                                                                                <  1:3571375/150‑1 (MQ=255)

                                                       gAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGt                                                                                               >  1:4606279/1‑150 (MQ=255)

                                                       gAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGt                                                                                               <  1:270951/150‑1 (MQ=255)

                                                        aaTGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAATGACGCTGTGTTTGTCGGt                                                                                               >  2:4440024/1‑150 (MQ=255)

                                                          tGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGa                                                                                             >  1:506534/1‑150 (MQ=255)

                                                           gCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGaa                                                                                            >  2:2344371/1‑150 (MQ=255)

                                                            ccGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAag                                                                                           <  1:3478555/150‑1 (MQ=255)

                                                            ccGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAag                                                                                           <  1:5262470/150‑1 (MQ=255)

                                                             cGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAaga                                                                                          <  1:4487349/150‑1 (MQ=255)

                                                              ggCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAagag                                                                                         >  2:2656057/1‑150 (MQ=255)

                                                              ggCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAACAGc                                                                                        <  1:4973078/150‑1 (MQ=255)

                                                                cTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCa                                                                                       <  1:4217350/150‑1 (MQ=255)

                                                                cTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCa                                                                                       <  2:3367945/150‑1 (MQ=255)

                                                                cTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCaa                                                                                      >  2:5031855/1‑150 (MQ=255)

                                                                 tttGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCaa                                                                                      >  1:3653242/1‑150 (MQ=255)

                                                                 tttGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCaa                                                                                      >  2:1528918/1‑150 (MQ=255)

                                                                    ggTTTGACAGACAATGTGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGCACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCACTCTg                                                                                  >  1:3125445/1‑150 (MQ=255)

                                                                         gacagacaATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTgcagca                                                                             <  1:5346526/150‑1 (MQ=255)

                                                                          acagacaATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTgcagca                                                                             >  2:813894/1‑150 (MQ=255)

                                                                          acagacaATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCAt                                                                            >  1:2432762/1‑150 (MQ=255)

                                                                            agacaATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCt                                                                          >  2:3595595/1‑150 (MQ=255)

                                                                                aaTGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCAt                                                                      >  1:4809823/1‑150 (MQ=255)

                                                                                 aTGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCAt                                                                      <  1:1021090/150‑1 (MQ=255)

                                                                                      tttGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATc                                                                 <  1:2879027/150‑1 (MQ=255)

                                                                                      tttGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATc                                                                 <  1:5287656/150‑1 (MQ=255)

                                                                                            atGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGc                                                          >  2:2748972/1‑150 (MQ=255)

                                                                                              ggTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCa                                                         >  2:1033786/1‑150 (MQ=255)

                                                                                                tgaaagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGAt                                                      <  1:5175716/150‑1 (MQ=255)

                                                                                                 gaaagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGAtt                                                     <  1:2063787/150‑1 (MQ=255)

                                                                                                  aaagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTc                                                    <  2:931057/150‑1 (MQ=255)

                                                                                                   aagtgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTc                                                    <  1:879320/150‑1 (MQ=255)

                                                                                                      tgaaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAAc                                                 >  1:2304937/1‑150 (MQ=255)

                                                                                                        aaagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCgg                                              <  2:2022211/150‑1 (MQ=255)

                                                                                                         aagCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCgg                                              <  2:2803884/150‑1 (MQ=255)

                                                                                                           gCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGt                                            <  1:1942071/150‑1 (MQ=255)

                                                                                                           gCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGtt                                           <  2:4589589/150‑1 (MQ=255)

                                                                                                              acacAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTtat                                         <  2:4859114/150‑1 (MQ=255)

                                                                                                                  agacagaACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATacac                                     >  1:3279961/1‑150 (MQ=255)

                                                                                                                  agacagaACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATacac                                     <  1:5726081/150‑1 (MQ=255)

                                                                                                                    acagaACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACtt                                   >  1:726482/1‑150 (MQ=255)

                                                                                                                     cagaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATGAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAGTCTGCAGCATCTGCATCAAACGTGTTGCAGATTCAAACGGGTTATACACGTGa                                 >  2:2109788/1‑150 (MQ=255)

                                                                                                                      agaACTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGAt                                >  2:3943346/1‑150 (MQ=255)

                                                                                                                        aaCTGTCAAACTTGATCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa                              <  1:1653166/150‑1 (MQ=255)

                                                                                                                         aCTGACAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCa                              <  1:836898/150‑1 (MQ=255)

                                                                                                                             tCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGc                          >  1:3362445/1‑150 (MQ=255)

                                                                                                                              cAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGcc                         >  2:737469/1‑150 (MQ=255)

                                                                                                                               aaaCTTGATCAATGAAATGGAC‑GTATTAAAAAATTAAAACTAATGATTAATCTGCCGAAAGACGCTGTGTTTGTCGGACAACAGCAATCTTCAGCATCGGCATCAACCGTTTTGCAGATTCAACCGGGTTATACCGTTGATCAAAGCCaa                       <  1:2239919/150‑1 (MQ=255)

                                                                                                                                 aCTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCaaa                      <  2:120560/150‑1 (MQ=255)

                                                                                                                                 aCTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCaaa                      >  2:1432237/1‑150 (MQ=255)

                                                                                                                                  cTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAAt                     <  1:3012071/150‑1 (MQ=255)

                                                                                                                                   ttGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATc                    <  1:3874840/150‑1 (MQ=255)

                                                                                                                                   ttGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTCAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATc                    <  1:1742276/150‑1 (MQ=255)

                                                                                                                                    tGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCa                   <  1:2261975/150‑1 (MQ=255)

                                                                                                                                    tGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTCGCAGATTCAACCGGGTTATACACTTGATCAAGGTCAAATCa                   >  2:5576433/1‑150 (MQ=255)

                                                                                                                                       tCAATGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACgg               <  1:4722636/150‑1 (MQ=255)

                                                                                                                                           tGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGAtt            <  2:1868336/150‑1 (MQ=255)

                                                                                                                                           tGAAATGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATta           <  2:5448590/150‑1 (MQ=255)

                                                                                                                                            gAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGa              <  1:4526459/147‑1 (MQ=255)

                                                                                                                                            gAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATta           <  1:4091706/150‑1 (MQ=255)

                                                                                                                                               aTGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGGAGGGCAATCTGCAGCATCTGCATCAATCGTGTTGCAGATTCAACCGGGTTATACACTTGGTCAAAGCCAAATCAACGGATTatat        >  2:2954125/1‑150 (MQ=255)

                                                                                                                                               aTGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATTAACGGATTatat        <  2:4706795/150‑1 (MQ=255)

                                                                                                                                               aTGGAC‑GTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATatc       <  2:1677478/150‑1 (MQ=255)

                                                                                                                                                 ggACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATatca      <  1:250585/150‑1 (MQ=255)

                                                                                                                                                  gACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATatcat     >  2:2537106/1‑150 (MQ=255)

                                                                                                                                                   aCGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATatcatc    <  1:1289723/150‑1 (MQ=255)

TGGCTGCAGAAGGACTTCCTAAAACGGGATCAATTGATTATTCGTTTTTTGGACAGAATGCCGGCTTTGGTTTGACAGACAATGAGTTTGATATGGTGAAAGTGAAAGCTACACAGACAGAACTGTCAAACTTGATCAATGAAATGGACGGTATTAAAAATTCAAAAGTAATGATTAATCTCCCGAAAGACGCTGTGTTTGTCGGTGAAGAGCAATCTGCAGCATCTGCATCAATCGTTTTGCAGATTCAACCGGGTTATACACTTGATCAAAGCCAAATCAACGGATTATATCATCTG  >  CP006881/1655829‑1656127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`