BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,211,770	A→G	14.4%	A22A (GCA→GCG)	U712_06040 →	Spore coat protein O

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,211,770	0	A	G	14.4%	748.7 / 122.3	309	A22A (GCA→GCG)	U712_06040	Spore coat protein O
Reads supporting (aligned to +/- strand): ref base A (152/112); new base G (29/16); total (181/128)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 311 total aligned reads displayed.

CCGAATAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAG  >  CP006881/1211621‑1211917

 cGAATAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAg                                                                                                                                                     >  2:4814048/1‑150 (MQ=255)

    aTAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTNATGTATATTGTTCAGCCGGATTATGCAGaaa                                                                                                                                                  >  1:1399490/1‑150 (MQ=255)

    aTAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGaaa                                                                                                                                                  >  1:439703/1‑150 (MQ=255)

      aGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACt                                                                                                                                                <  1:5078573/150‑1 (MQ=255)

        ggATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGAAGGGATGAAGATGTCTAACAACAAAAGAAACCAGGATAAAAAACCAATCATGTATATTGTTCAGCCGGATTATGCAGTAACTAc                                                                                                                                              >  2:566351/1‑150 (MQ=255)

           ttGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAg                                                                                                                                           >  2:5191065/1‑150 (MQ=255)

           ttGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAg                                                                                                                                           >  1:1099318/1‑150 (MQ=255)

            tGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAgg                                                                                                                                          >  1:651909/1‑150 (MQ=255)

            tGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAgg                                                                                                                                          >  2:3771940/1‑150 (MQ=255)

             gcgtttttATATCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGt                                                                                                                                         >  2:2589168/4‑150 (MQ=255)

             gCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGt                                                                                                                                         >  1:4245028/1‑150 (MQ=255)

                tttttATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATAACACAATCGGAGAGATGAAGATCTCTAATAAAAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATTCGGAAACTACAAGGTCAt                                                                                                                                      >  1:90803/1‑150 (MQ=255)

                  tttATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAATCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATcc                                                                                                                                    <  2:1951399/150‑1 (MQ=255)

                  tttATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATcc                                                                                                                                    <  1:3387418/150‑1 (MQ=255)

                    tATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCAt                                                                                                                                  >  1:4435581/1‑150 (MQ=255)

                     aTTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATg                                                                                                                                 >  1:1142183/1‑150 (MQ=255)

                        tcttctGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAg                                                                                                                              >  1:1677275/1‑150 (MQ=255)

                         cttctGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAgg                                                                                                                             <  2:2793170/150‑1 (MQ=255)

                           tctGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGgag                                                                                                                           >  1:2975424/1‑150 (MQ=255)

                              gATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGAAGAtt                                                                                                                        >  2:5109565/1‑150 (MQ=255)

                                 tttCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGtc                                                                                                                     <  1:5221487/150‑1 (MQ=255)

                                 tttCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGtc                                                                                                                     >  1:3341221/1‑150 (MQ=255)

                                 tttCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGtc                                                                                                                     <  2:1233327/150‑1 (MQ=255)

                                   tCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGtcat                                                                                                                   <  1:5007280/150‑1 (MQ=255)

                                    cAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGtcatc                                                                                                                  >  2:1995004/1‑150 (MQ=255)

                                     aGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGtcatca                                                                                                                 <  2:5421928/150‑1 (MQ=255)

                                      gCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaa                                                                                                                <  2:4277805/150‑1 (MQ=255)

                                       cTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGTATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGAAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaaa                                                                                                               <  1:1909896/150‑1 (MQ=255)

                                       cTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaaa                                                                                                               >  2:5330268/1‑150 (MQ=255)

                                       cTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaaa                                                                                                               <  1:537219/150‑1 (MQ=255)

                                       cTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaaa                                                                                                               >  2:5009608/1‑150 (MQ=255)

                                       cTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCAAGCAGGAGATTGTCATCaaa                                                                                                               >  2:732821/1‑150 (MQ=255)

                                        tttCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCaaaa                                                                                                              >  1:3991933/1‑150 (MQ=255)

                                          tCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAaaaga                                                                                                            >  2:1336819/1‑150 (MQ=255)

                                            tGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAaaagaaa                                                                                                          >  1:5646826/1‑150 (MQ=255)

                                             gTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAaaagaagg                                                                                                         >  2:620603/1‑148 (MQ=255)

                                              tCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAgg                                                                                                        <  1:5289952/150‑1 (MQ=255)

                                                atatAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGcc                                                                                                      <  1:2103229/150‑1 (MQ=255)

                                                   tatatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGAc                                                                                                   >  2:3026486/4‑150 (MQ=255)

                                                   tagatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGAc                                                                                                   >  2:2305222/1‑150 (MQ=255)

                                                    agatagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACa                                                                                                  >  2:3932524/1‑150 (MQ=255)

                                                      atagaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACaaa                                                                                                >  2:4095054/1‑150 (MQ=255)

                                                        agaATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAGCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAAcc                                                                                              <  1:4557375/150‑1 (MQ=255)

                                                          aaTATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCg                                                                                            >  1:2675856/1‑150 (MQ=255)

                                                             atGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGACGACAAACCCGCTc                                                                                         >  2:4870098/1‑150 (MQ=255)

                                                             atGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTc                                                                                         <  1:2270982/150‑1 (MQ=255)

                                                              tGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTcc                                                                                        <  2:2188645/150‑1 (MQ=255)

                                                               gACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCg                                                                                       <  1:2492851/150‑1 (MQ=255)

                                                                       cGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGc                                                                               >  2:2469682/1‑150 (MQ=255)

                                                                       cGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGc                                                                               <  1:1742048/150‑1 (MQ=255)

                                                                         gAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTg                                                                             >  1:4501695/1‑150 (MQ=255)

                                                                          aGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGa                                                                            >  2:3614025/1‑150 (MQ=255)

                                                                           gggATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAg                                                                           <  2:380621/150‑1 (MQ=255)

                                                                            ggATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAgg                                                                          <  1:3771940/150‑1 (MQ=255)

                                                                            ggATGAAGATGCCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTGCAAGGCCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCGGCCAAAAGCTGAgg                                                                          <  2:5699606/150‑1 (MQ=255)

                                                                             gATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGa                                                                         >  1:4262501/1‑150 (MQ=255)

                                                                              aTGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGaa                                                                        <  1:2779522/150‑1 (MQ=255)

                                                                                gAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGaaaa                                                                      >  2:331696/1‑150 (MQ=255)

                                                                                 aaGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAg                                                                     >  2:4684920/1‑150 (MQ=255)

                                                                                  aGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGc                                                                    <  1:2305222/150‑1 (MQ=255)

                                                                                   gATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGcc                                                                   >  2:3245312/1‑150 (MQ=255)

                                                                                       tCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCt                                                               <  1:3612724/150‑1 (MQ=255)

                                                                                          aataaGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTAc                                                            >  2:3475755/1‑150 (MQ=255)

                                                                                             aagaaaagaaACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCAt                                                         <  1:884747/150‑1 (MQ=255)

                                                                                              agaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATg                                                        >  2:1525375/1‑150 (MQ=255)

                                                                                              agaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATg                                                        <  2:1099318/150‑1 (MQ=255)

                                                                                              agaaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATg                                                        >  2:3100533/1‑150 (MQ=255)

                                                                                                aaaagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATgag                                                      >  2:5813123/1‑150 (MQ=255)

                                                                                                  aagaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGaa                                                    <  1:1018867/150‑1 (MQ=255)

                                                                                                  aagaaaCGCGGATAAAAAACCGCTCATGTAAATTGATCAGCCGGATTATGGGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCTACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCAGCTTACCATgtcaa                                                    >  2:2545817/1‑146 (MQ=255)

                                                                                                   agaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAag                                                   <  1:3480763/150‑1 (MQ=255)

                                                                                                    gaaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATGAAAAGAAAGGCGGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGGCGGCTTACCATGAGAagc                                                  <  1:3832576/150‑1 (MQ=255)

                                                                                                     aaaCGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAagca                                                 <  1:1593456/150‑1 (MQ=255)

                                                                                                         gcgGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGcagcag                                             >  2:43554/1‑150 (MQ=255)

                                                                                                          cgGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCGGAAACTACAAGATCATCCATGCAGGACATTGTCATCAAAAGAAAGGCCGACAACCCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCagg                                            >  2:1775115/1‑150 (MQ=255)

                                                                                                          cgGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCagg                                            >  1:2725853/1‑150 (MQ=255)

                                                                                                            gATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCagg‑ag                                         >  1:5482405/1‑150 (MQ=255)

                                                                                                                aaaaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAg                                     >  2:4938421/1‑150 (MQ=255)

                                                                                                                  aaaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGcc                                   <  2:2739800/150‑1 (MQ=255)

                                                                                                                   aaCCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCg                                  >  1:1611066/1‑150 (MQ=255)

                                                                                                                      cGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTgg                               <  1:5561001/150‑1 (MQ=255)

                                                                                                                      cGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTgg                               <  1:4940820/150‑1 (MQ=255)

                                                                                                                       gCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGc                              >  2:2646008/1‑150 (MQ=255)

                                                                                                                          cATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCC‑AAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAg                          >  2:83523/1‑150 (MQ=255)

                                                                                                                           aTGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAg                          >  2:2668926/1‑150 (MQ=255)

                                                                                                                           aTGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAg                          <  1:3671707/150‑1 (MQ=255)

                                                                                                                           aTGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAACCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAg                          >  1:1974694/1‑150 (MQ=255)

                                                                                                                              tataTTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGAt                       >  2:2197611/1‑150 (MQ=255)

                                                                                                                                 atTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTc                    >  2:3394825/1‑150 (MQ=255)

                                                                                                                                  ttGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTcc                   <  1:2849873/150‑1 (MQ=255)

                                                                                                                                    gTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGTCAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTCGCACAGGATGTCCTg                 <  2:1386162/150‑1 (MQ=255)

                                                                                                                                     ttCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGc                >  1:1923122/1‑150 (MQ=255)

                                                                                                                                       cAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCaa              <  2:4501695/150‑1 (MQ=255)

                                                                                                                                        aGCCGGATTATGCGGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAGAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCaag             <  1:1313003/150‑1 (MQ=255)

                                                                                                                                         gCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCaaga            >  2:4572339/1‑150 (MQ=255)

                                                                                                                                          ccGGATTATGCAGAAACTCCAAGGCCCTCCATCCAGGAGATTGTCATCAAAAGAAAGGCCGCCAAACCCGCTCCGCCAAAACCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCaagaa           <  2:1677275/150‑1 (MQ=255)

                                                                                                                                          ccGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCaagaa           <  1:1229385/150‑1 (MQ=255)

                                                                                                                                            ggATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGGGAAACCCGCTCCGCCAAAAGCTTAAGAAAAGCCGGCTTACCATGAGAAGCAGGAGGAAGGAAG‑CGTGGCACAGGATGACCTGGAagaaga         >  1:4743548/1‑150 (MQ=255)

                                                                                                                                              aTTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGAcc       <  2:5482405/150‑1 (MQ=255)

                                                                                                                                                   gCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAg  <  1:3063853/150‑1 (MQ=255)

CCGAATAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAGAATATGACACAATCGGAGGGATGAAGATGTCTAATAAGAAAAGAAACGCGGATAAAAAACCGCTCATGTATATTGTTCAGCCGGATTATGCAGAAACTACAAGGTCATCCATGCAGGAGATTGTCATCAAAAGAAAGGCCGACAAACCCGCTCCGCCAAAAGCTGAGGAAAAGCCGGCTTACCATGAGAAGCAGCAGG‑AGGAAGCCGTGGCACAGGATGTCCTGCAAGAAGACCAAAAG  >  CP006881/1211621‑1211917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`