Predicted mutation
evidence seq id position mutation freq annotation gene description
RA pSIJ663_pHT315_araE_xy 2,146 A→G 16.2% intergenic (–/‑832) – / → eryR –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*pSIJ663_pHT315_araE_xy2,1460AG16.2% 66529.3 / inf 28294intergenic (–/‑832)–/eryR–/–
Reads supporting (aligned to +/- strand):  ref base A (11567/12107);  new base G (2235/2344);  total (13825/14469)
Fisher's exact test for biased strand distribution p-value = 9.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 28764 total aligned reads displayed.

TCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGA  >  pSIJ663_pHT315_araE_xy/1996‑2296
                                                                                                                                                      |                                                                                                                                                      
  ggggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAg                                                                                                                                                       >  2:2145785/1‑150 (MQ=255)
     gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                                                                                                                                                    >  1:3636662/1‑150 (MQ=255)
      tgtTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGtttt                                                                                                                                                   <  1:1232358/150‑1 (MQ=255)
      tgtTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGtttt                                                                                                                                                   >  2:4187848/1‑150 (MQ=255)
        ttCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGc                                                                                                                                                 >  1:3979354/1‑150 (MQ=255)
         tCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGcc                                                                                                                                                <  2:4369253/150‑1 (MQ=255)
             cGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCacaa                                                                                                                                            >  2:1614111/1‑150 (MQ=255)
              gATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                                                                                                                                           <  2:3694775/150‑1 (MQ=255)
              gATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGACAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                                                                                                                                           >  1:1917151/1‑150 (MQ=255)
              gATATCTTTTATGTACGATAAATATTTTTCATAAATACCAGGATGTAAATTCTTAACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGGAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                                                                                                                                           >  1:2253373/1‑150 (MQ=255)
                tatCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAg                                                                                                                                         <  1:2993634/150‑1 (MQ=255)
                 atCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGt                                                                                                                                        >  1:3101287/1‑150 (MQ=255)
                     tttATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGt                                                                                                                                    <  1:2034685/150‑1 (MQ=255)
                     tttATGTGCGATGAATATTTTTCATAAATACCAGGATGTCGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGt                                                                                                                                    <  1:5463648/150‑1 (MQ=255)
                       tATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGttt                                                                                                                                  <  1:4400837/150‑1 (MQ=255)
                        aTGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGttta                                                                                                                                 >  2:1186432/1‑150 (MQ=255)
                         tgtgCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtttat                                                                                                                                <  1:320006/150‑1 (MQ=255)
                           tgCGATGAATATTTTTCATAAATACCAGGATGATGTTTCTTTACGTGCTTTATAAATCCGGTAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtttattt                                                                                                                              >  2:3080484/1‑150 (MQ=255)
                             cGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTAc                                                                                                                            >  1:1112330/1‑150 (MQ=255)
                              gATGAATATTTTTCATATATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACa                                                                                                                           <  1:3518657/150‑1 (MQ=255)
                              gATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACa                                                                                                                           >  2:1086460/1‑150 (MQ=255)
                                tGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAt                                                                                                                         <  2:5752759/150‑1 (MQ=255)
                                tGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAAt                                                                                                                         >  1:3299509/1‑150 (MQ=255)
                                 gAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGTAAACATTTTTACATCGTTAGAAGTGCTCGTCCAGTATTATGTAATTATAATGATTTGTGAAATTTTTGCCACAACAGTTGGTTTATTTACAAta                                                                                                                        >  1:580030/1‑149 (MQ=255)
                                   atatTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtt                                                                                                                      >  1:759551/1‑150 (MQ=255)
                                    tatTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCttt                                                                                                                     <  2:2863085/150‑1 (MQ=255)
                                     atTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtttt                                                                                                                    <  1:3876177/150‑1 (MQ=255)
                                      tttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCTGGAAAAATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCttttt                                                                                                                   >  1:1573796/1‑150 (MQ=255)
                                      tttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCttttt                                                                                                                   <  1:3765423/150‑1 (MQ=255)
                                      tttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCttttt                                                                                                                   <  2:2049837/150‑1 (MQ=255)
                                       ttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCtttttt                                                                                                                  <  2:2459375/150‑1 (MQ=255)
                                       ttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtttttt                                                                                                                  >  1:3405104/1‑150 (MQ=255)
                                       ttttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtttttt                                                                                                                  >  2:3492366/1‑150 (MQ=255)
                                        tttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCttttttt                                                                                                                 <  1:4700772/150‑1 (MQ=255)
                                         ttCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCttttttta                                                                                                                <  2:236550/150‑2 (MQ=255)
                                           cATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtttttttatt                                                                                                              >  1:1352995/1‑147 (MQ=255)
                                           cATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTttat                                                                                                              <  2:547229/150‑1 (MQ=255)
                                            aTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTttatt                                                                                                             <  2:4671377/150‑1 (MQ=255)
                                             tAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAg                                                                                                           >  1:5017927/1‑150 (MQ=255)
                                             tAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAg                                                                                                           <  2:1832716/150‑1 (MQ=255)
                                                aTACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGcc                                                                                                         >  2:2763891/1‑150 (MQ=255)
                                                     aGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATAATTTGTGGAAGTATTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCaaa                                                                                                   >  1:4501547/1‑150 (MQ=255)
                                                      ggATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAAt                                                                                                  <  1:4300803/150‑1 (MQ=255)
                                                           ttgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTtctc                                                                                             >  1:4606019/1‑150 (MQ=255)
                                                           ttgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTtctc                                                                                             >  1:2880756/1‑150 (MQ=255)
                                                           ttgttTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGAAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTtct                                                                                              >  1:2687861/1‑150 (MQ=255)
                                                              tttCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTGAAATTTCTccc                                                                                           >  1:5576415/1‑150 (MQ=255)
                                                                                aaaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTtata                                                                         <  1:1458833/150‑1 (MQ=255)
                                                                                aaaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTtata                                                                         >  2:786291/1‑150 (MQ=255)
                                                                                aaaTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTatat                                                                        <  2:894365/150‑1 (MQ=255)
                                                                                  aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATc                                                                       <  2:5281990/150‑1 (MQ=255)
                                                                                    ccGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCttt                                                                    >  2:4764619/1‑150 (MQ=255)
                                                                                      gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCttta                                                                   >  2:49630/1‑150 (MQ=255)
                                                                                      gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCttta                                                                   <  2:31799/150‑1 (MQ=255)
                                                                                       ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttat                                                                  <  2:1514291/150‑1 (MQ=255)
                                                                                         aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttt                                                               <  2:3792972/150‑1 (MQ=255)
                                                                                         aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttt                                                               >  1:4912138/1‑150 (MQ=255)
                                                                                           aCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttta                                                              <  2:680189/150‑1 (MQ=255)
                                                                                           aCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAACTTCTCCCTCATCTCGTCTCTTTATTTCtttattttat                                                             >  2:1939192/1‑150 (MQ=255)
                                                                                            cATTTTTACATCGTTTGAAGTGCTAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGACACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGTCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattttat                                                             <  1:5134493/150‑1 (MQ=255)
                                                                                            cATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattttat                                                             >  2:3379419/1‑150 (MQ=255)
                                                                                               ttttACATCGTTAGATTGTCAAGTCAAGTTATATGTATTTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTCCAACCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCAt                                                          <  1:2479342/150‑1 (MQ=255)
                                                                                               ttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCAt                                                          >  2:2971311/1‑150 (MQ=255)
                                                                                               ttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCAt                                                          <  1:5351651/150‑1 (MQ=255)
                                                                                                tttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATa                                                         <  2:584891/150‑1 (MQ=255)
                                                                                                 ttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                                        <  1:2369954/150‑1 (MQ=255)
                                                                                                    cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                    >  2:3592414/1‑150 (MQ=255)
                                                                                                     aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAgaa                                                    >  1:1679813/1‑148 (MQ=255)
                                                                                                     aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                    <  1:87334/150‑1 (MQ=255)
                                                                                                       cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                                  >  2:4278650/1‑150 (MQ=255)
                                                                                                       cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                                  <  2:2666830/150‑1 (MQ=255)
                                                                                                           aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAttt                                              >  2:4093894/1‑150 (MQ=255)
                                                                                                            gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTg                                             <  2:4573651/150‑1 (MQ=255)
                                                                                                               gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                          >  2:1721758/1‑150 (MQ=255)
                                                                                                               gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                          >  1:4199133/1‑150 (MQ=255)
                                                                                                                    agtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                     <  2:5566287/150‑1 (MQ=255)
                                                                                                                       caagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgcg                                 >  2:1296440/1‑150 (MQ=255)
                                                                                                                        aagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgcg                                 >  2:3987362/1‑150 (MQ=255)
                                                                                                                        aagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcgcg                                 >  2:2254788/1‑150 (MQ=255)
                                                                                                                             atatGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACa                            <  2:1403952/150‑1 (MQ=255)
                                                                                                                                  tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt                       <  1:4095777/150‑1 (MQ=255)
                                                                                                                                  tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                      <  1:1806409/150‑1 (MQ=255)
                                                                                                                                   aTCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                      >  2:3276249/1‑150 (MQ=255)
                                                                                                                                    tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                     <  1:5689011/150‑1 (MQ=255)
                                                                                                                                     cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                    >  2:5068595/1‑150 (MQ=255)
                                                                                                                                     cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                    <  2:4465178/150‑1 (MQ=255)
                                                                                                                                       ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                  >  1:2781870/1‑150 (MQ=255)
                                                                                                                                        taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATTTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATa                 <  1:4806661/150‑1 (MQ=255)
                                                                                                                                         aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                <  2:5376636/150‑1 (MQ=255)
                                                                                                                                         aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                <  1:640178/150‑1 (MQ=255)
                                                                                                                                         aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                >  1:1000182/1‑150 (MQ=255)
                                                                                                                                         aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAgg               <  2:2560825/150‑1 (MQ=255)
                                                                                                                                            gATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGa             >  2:5176641/1‑150 (MQ=255)
                                                                                                                                              tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGatat          <  2:3896007/150‑1 (MQ=255)
                                                                                                                                                 gtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAtttt       <  2:4871580/150‑1 (MQ=255)
                                                                                                                                                  ttgAAGTTTTGCCACATCAGTTGGTTTATTTACATTTTTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTTGTCTCTTTATATCTTTATTTTATCATTAAGGAGTATTTCAACCGTCGCGCGGGACAGCTTTATGATAGGGATAtttt       <  1:987837/148‑1 (MQ=255)
                                                                                                                                                   ggAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa      <  1:2580676/150‑1 (MQ=255)
                                                                                                                                                   ggAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCTTATGCCTGTCTTCCCCTCCTCCCCCCGCACAGGTTTATGATAGGGATATTTTa      >  1:214429/1‑150 (MQ=255)
                                                                                                                                                      aGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTg   >  1:5241848/1‑150 (MQ=255)
                                                                                                                                                      aGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTg   >  2:5656575/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGA  >  pSIJ663_pHT315_araE_xy/1996‑2296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑