Predicted mutation
evidence seq id position mutation freq annotation gene description
RA pSIJ663_pHT315_araE_xy 2,214 C→T 44.5% intergenic (–/‑764) – / → eryR –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*pSIJ663_pHT315_araE_xy2,2140CT44.5% 17742.8 / inf 27293intergenic (–/‑764)–/eryR–/–
Reads supporting (aligned to +/- strand):  ref base C (7927/7194);  new base T (6348/5772);  total (14295/12998)
Fisher's exact test for biased strand distribution p-value = 9.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 0.00e+00

Only 100 of 27945 total aligned reads displayed.

TACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACA  >  pSIJ663_pHT315_araE_xy/2064‑2363
                                                                                                                                                      |                                                                                                                                                     
tACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAtctc                                                                                                                                                       <  2:1457487/150‑1 (MQ=255)
      cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGtctc                                                                                                                                                  <  2:1551635/150‑1 (MQ=255)
              aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCt                                                                                                                                         <  2:1358718/150‑1 (MQ=255)
              aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCt                                                                                                                                         <  2:3108214/150‑1 (MQ=255)
               tCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGACACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTATCTTTATATCt                                                                                                                                         <  1:5702702/150‑1 (MQ=255)
                  gggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCttta                                                                                                                                      <  2:406321/150‑1 (MQ=255)
                   ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttat                                                                                                                                     >  1:1229110/1‑150 (MQ=255)
                    gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatt                                                                                                                                    <  2:28089/150‑1 (MQ=255)
                    gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatt                                                                                                                                    <  1:2684111/150‑1 (MQ=255)
                     aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattt                                                                                                                                   <  2:5143113/150‑1 (MQ=255)
                      aaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttatttt                                                                                                                                  <  2:4250275/150‑1 (MQ=255)
                        cATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTT‑TTAGCCGTCAAATTTCTCCCTCAACTCGTCTCTTTATATCTTTATTTTATc                                                                                                                               <  2:3302466/150‑1 (MQ=255)
                        cATTTTTACATCGTTAGAAGTGCAAGCCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCtttattttat                                                                                                                                <  2:2712758/150‑1 (MQ=255)
                         aTTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATc                                                                                                                               >  1:2084016/1‑150 (MQ=255)
                           ttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCAt                                                                                                                             <  2:5244812/150‑1 (MQ=255)
                            tttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGTAGTTTTGCCACAACAGTTGGTTTATTTACAATCATTTTTTTATTAGCCGTCAAATTTCTCTCTTATTTCGTCTCTTTATATCTTTATTTTATCATa                                                                                                                            <  1:2204911/150‑1 (MQ=255)
                            tttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATa                                                                                                                            <  2:3877673/150‑1 (MQ=255)
                            tttACATCATTAGAAGTGCAACTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATa                                                                                                                            >  1:323806/1‑150 (MQ=255)
                               aCATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAg                                                                                                                         <  1:1591035/150‑1 (MQ=255)
                                 aTCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                                       >  1:4684949/1‑150 (MQ=255)
                                   cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                                                                                                     >  1:1717976/1‑150 (MQ=255)
                                     ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGATTATTTACAATCTTATTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAt                                                                                                                   >  1:1108241/1‑150 (MQ=255)
                                      tAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAtt                                                                                                                  >  1:3125292/1‑150 (MQ=255)
                                       aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAttt                                                                                                                 >  1:5585296/1‑150 (MQ=255)
                                        gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGa                                                                                                               <  1:3821253/150‑1 (MQ=255)
                                          aGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAc                                                                                                             <  2:5336441/150‑1 (MQ=255)
                                            tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAAcc                                                                                                            <  2:2620472/150‑1 (MQ=255)
                                              caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTGTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGt                                                                                                          >  1:94469/1‑150 (MQ=255)
                                                agtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                                                                        <  2:5514774/150‑1 (MQ=255)
                                                agtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                                                                                                        >  2:4838538/1‑150 (MQ=255)
                                                 gtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgc                                                                                                       <  2:2136250/150‑1 (MQ=255)
                                                      gtTATATGTATCTATAATGATTTCTGGAAGTTTTGCCACAACAGTTGCTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                                                                                                  >  1:5252621/1‑150 (MQ=255)
                                                          tatGTATCTATATTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATGTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAg                                                                                              <  1:2449724/150‑1 (MQ=255)
                                                            tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGt                                                                                            <  1:2791796/150‑1 (MQ=255)
                                                             gTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                                           <  2:1949154/150‑1 (MQ=255)
                                                             gTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt                                                                                          <  2:804066/150‑1 (MQ=255)
                                                                 cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                                      >  1:3347903/1‑150 (MQ=255)
                                                                  tataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTAACATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                                     <  2:4444212/150‑1 (MQ=255)
                                                                   ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                                     >  1:4809809/1‑150 (MQ=255)
                                                                     aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                                   >  1:1000182/1‑150 (MQ=255)
                                                                     aaTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                                   <  1:3583922/150‑1 (MQ=255)
                                                                      aTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAgg                                                                                  >  1:185331/1‑150 (MQ=255)
                                                                       tGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAggg                                                                                 >  1:4253142/1‑150 (MQ=255)
                                                                         aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGata                                                                              >  1:255812/1‑150 (MQ=255)
                                                                          tttGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGAAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGata                                                                              >  1:3017398/1‑150 (MQ=255)
                                                                          tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGata                                                                              >  1:4037407/1‑150 (MQ=255)
                                                                               ggAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTAt                                                                        >  1:1964536/1‑150 (MQ=255)
                                                                                gAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTAt                                                                        >  2:5422185/1‑150 (MQ=255)
                                                                                  aGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGa                                                                     >  2:3964960/1‑150 (MQ=255)
                                                                                        gCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaataat                                                                <  1:5141796/150‑1 (MQ=255)
                                                                                          cacaACAGTTGGTTTATTTACAATCTTTTTTTTATTAGGCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAATCGTAGCGCCGCACAGGTTTATGATAGGGATATTTTATTGAATAATTg                                                              <  1:4920574/150‑1 (MQ=255)
                                                                                          cacaACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTg                                                              <  2:3775804/150‑1 (MQ=255)
                                                                                             aacaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg                                                           <  2:115674/150‑1 (MQ=255)
                                                                                             aacaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATg                                                           >  2:3490876/1‑150 (MQ=255)
                                                                                              acaGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATgg                                                          <  2:898703/150‑1 (MQ=255)
                                                                                               caGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGt                                                         >  2:1758252/1‑150 (MQ=255)
                                                                                               caGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGt                                                         >  2:1059191/1‑150 (MQ=255)
                                                                                                 gTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGtat                                                       >  2:1255636/1‑150 (MQ=255)
                                                                                                  ttGGTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATaa                                                     >  2:4933597/1‑150 (MQ=255)
                                                                                                    ggTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                                   <  2:2908392/150‑1 (MQ=255)
                                                                                                    ggTTTATTTACAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                                   >  2:22330/1‑150 (MQ=255)
                                                                                                     gTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                                   <  2:3204009/150‑1 (MQ=255)
                                                                                                      tttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAggg                                                  <  2:251890/150‑1 (MQ=255)
                                                                                                      tttatttaCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGa                                                 <  2:2045073/150‑1 (MQ=255)
                                                                                                       ttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGa                                                 >  1:4145177/1‑150 (MQ=255)
                                                                                                       ttatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGa                                                 >  2:473984/1‑150 (MQ=255)
                                                                                                        tatttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGAc                                                <  2:3685322/150‑1 (MQ=255)
                                                                                                        tatttaCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACt                                               <  1:682278/150‑1 (MQ=255)
                                                                                                         atttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAGGGGACt                                               >  2:611580/1‑150 (MQ=255)
                                                                                                          tttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACtt                                              >  1:5360754/1‑150 (MQ=255)
                                                                                                          tttaCAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACtt                                              >  2:503652/1‑150 (MQ=255)
                                                                                                            taCAATC‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCa                                           >  2:712320/1‑150 (MQ=255)
                                                                                                               aaTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATg                                         >  2:5788719/1‑150 (MQ=255)
                                                                                                               aaTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATg                                         <  1:5821291/150‑1 (MQ=255)
                                                                                                                aTCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGc                                        >  2:1644206/1‑150 (MQ=255)
                                                                                                                  cTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCtt                                      <  2:185163/150‑1 (MQ=255)
                                                                                                                      tttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGaa                                  <  1:4578492/150‑1 (MQ=255)
                                                                                                                       ttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGaaa                                 >  2:2423943/1‑150 (MQ=255)
                                                                                                                          tattaGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTg                              >  1:3467605/1‑150 (MQ=255)
                                                                                                                             taGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTgggg                           <  2:5604411/150‑1 (MQ=255)
                                                                                                                             taGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTgggg                           >  2:4349906/1‑150 (MQ=255)
                                                                                                                                ccGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGAtt                        >  1:3924951/1‑150 (MQ=255)
                                                                                                                                ccGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGAtt                        <  2:4541801/150‑1 (MQ=255)
                                                                                                                                ccGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCATCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGAtt                        <  1:1472305/150‑1 (MQ=255)
                                                                                                                                  gTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTAt                      <  2:2334665/150‑1 (MQ=255)
                                                                                                                                  gTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTAt                      >  2:4648554/1‑150 (MQ=255)
                                                                                                                                   tCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGGACCGTCGCGCGGGACAGGTTTATGATAGGAATATTTTATTGAATAATTCATGGTATAAGGGACTTTCATGCTTGGATAGTGTGGATTATg                     >  1:547129/1‑150 (MQ=255)
                                                                                                                                     aaaTTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGAGACAGGTTTATGATAGGAATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGaa                   >  1:5023084/1‑150 (MQ=255)
                                                                                                                                      aaTTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAt                  <  1:135048/150‑1 (MQ=255)
                                                                                                                                      aaTTTCTACCTCATCTTGTCTCTTTATATCTTTTTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAt                  <  1:3702249/150‑1 (MQ=255)
                                                                                                                                       aTTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAtt                 >  1:2320367/1‑150 (MQ=255)
                                                                                                                                       aTTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAAtt                 <  2:3397691/150‑1 (MQ=255)
                                                                                                                                        ttgctcactcaTCTCGTCACTTTACATCTTTATTTTATCATAAAGAAATATTTGAAACGTCGCGCGCGACAGGTTTATGATAAGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGAAGGATTTATGAATTa                >  1:4468254/4‑150 (MQ=255)
                                                                                                                                         ttCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAg               >  2:301204/1‑150 (MQ=255)
                                                                                                                                          tctctctcATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGa              <  2:4160595/150‑1 (MQ=255)
                                                                                                                                             cccTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGc           >  1:4361719/1‑150 (MQ=255)
                                                                                                                                               cTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCtt         <  2:5695245/150‑1 (MQ=255)
                                                                                                                                                 cATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGt       >  2:3584248/1‑150 (MQ=255)
                                                                                                                                                     tcGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCcac   >  1:80852/1‑150 (MQ=255)
                                                                                                                                                      cGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCcaca  <  1:2894940/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATGAATTAGATGCTTGTCCACA  >  pSIJ663_pHT315_araE_xy/2064‑2363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑