Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 35,960 A→G 10.9% Y39C (TAT→TGT)  U712_00130 → Protein xpaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00688135,9600AG10.9% 1156.2 / 121.5 434Y39C (TAT→TGT) U712_00130Protein xpaC
Reads supporting (aligned to +/- strand):  ref base A (195/190);  new base G (23/25);  total (219/215)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 439 total aligned reads displayed.

AAAACTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCGTCTTAGGA  >  CP006881/35811‑36109
                                                                                                                                                     |                                                                                                                                                     
aaaaCTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTa                                                                                                                                                       >  1:879052/1‑150 (MQ=255)
 aaaCTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTAt                                                                                                                                                      >  1:3878936/1‑150 (MQ=255)
 aaaCTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGANTTTTTTATCTATTGTTTAt                                                                                                                                                      >  2:5729607/1‑150 (MQ=255)
  aaCTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATg                                                                                                                                                     <  1:2637941/150‑1 (MQ=255)
      gAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGgct                                                                                                                                                 >  1:5108620/1‑150 (MQ=255)
          ataGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGgcttgct                                                                                                                                             <  2:2718383/150‑1 (MQ=255)
          ataGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTAAAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGAAATATTGTGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGtgcttgct                                                                                                                                             >  2:3057533/1‑150 (MQ=255)
           taGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCCACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGgcttgctt                                                                                                                                            >  1:186108/1‑150 (MQ=255)
              acacAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTg                                                                                                                                         <  2:4368681/150‑1 (MQ=255)
              acacAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTg                                                                                                                                         <  2:873834/150‑1 (MQ=255)
               cacaGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTGTGGGCTTGCTTCTgg                                                                                                                                        >  2:5072832/1‑150 (MQ=255)
               cacaGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTgg                                                                                                                                        <  2:2544745/150‑1 (MQ=255)
               cacaGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTgg                                                                                                                                        >  2:3801139/1‑150 (MQ=255)
                acaGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGa                                                                                                                                       <  1:1047611/150‑1 (MQ=255)
                 caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                                      <  1:5064034/150‑1 (MQ=255)
                    gAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcag                                                                                                                                   >  1:2025580/1‑150 (MQ=255)
                    gAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcag                                                                                                                                   <  1:3405496/150‑1 (MQ=255)
                     aGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcagc                                                                                                                                  >  1:2096716/1‑150 (MQ=255)
                     aGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcagc                                                                                                                                  >  1:3612429/1‑150 (MQ=255)
                         gATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGta                                                                                                                              <  2:1397325/150‑1 (MQ=255)
                           ttATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGGTTGCTTCTGGAGCAGCCGtata                                                                                                                            >  1:5570162/1‑150 (MQ=255)
                             aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGtatata                                                                                                                          <  2:3553897/150‑1 (MQ=255)
                              tctcATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGtatatat                                                                                                                         <  1:583822/150‑1 (MQ=255)
                                 cATGCAGAGATTTTTTCTCTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGc                                                                                                                      <  2:4077450/150‑1 (MQ=255)
                                   tGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTg                                                                                                                    <  2:5387925/150‑1 (MQ=255)
                                   tGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTg                                                                                                                    >  2:3263102/1‑150 (MQ=255)
                                      agagATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCa                                                                                                                 >  2:4830168/1‑150 (MQ=255)
                                       gagaTTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGCAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTCTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCAt                                                                                                                <  2:3181167/150‑1 (MQ=255)
                                        agaTTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATc                                                                                                               >  1:138437/1‑150 (MQ=255)
                                          aTTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTg                                                                                                             <  2:2813435/150‑1 (MQ=255)
                                                tCACTNTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGc                                                                                                       >  1:5734723/1‑150 (MQ=255)
                                                 cACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTCCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCa                                                                                                      <  1:413092/150‑1 (MQ=255)
                                                  aCTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCaa                                                                                                     <  1:2346982/150‑1 (MQ=255)
                                                   cTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAg                                                                                                    >  1:4996768/1‑150 (MQ=255)
                                                       ttGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACgg                                                                                                <  2:1503653/150‑1 (MQ=255)
                                                       ttGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACgg                                                                                                <  2:5271073/150‑1 (MQ=255)
                                                        tGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGt                                                                                               >  2:5519218/1‑150 (MQ=255)
                                                            gtgGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTAtt                                                                                           <  2:1599120/150‑1 (MQ=255)
                                                             tgGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTAttt                                                                                          <  1:3574539/150‑1 (MQ=255)
                                                             tgGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTAttt                                                                                          >  2:2047330/1‑150 (MQ=255)
                                                             tgGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATCCAAGACGGTTAttt                                                                                          <  1:4585566/150‑1 (MQ=255)
                                                                aGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGAGGGTTATTTCtt                                                                                       <  2:1697209/150‑1 (MQ=255)
                                                                 gTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGGCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTa                                                                                      >  2:3882735/1‑150 (MQ=255)
                                                                   ttAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTaaa                                                                                    <  1:5683045/150‑1 (MQ=255)
                                                                         aGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCAt                                                                              <  2:5137877/150‑1 (MQ=255)
                                                                                  aCTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACa                                                                     >  1:4520489/1‑150 (MQ=255)
                                                                                    ttttGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTACCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATCCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAgg                                                                   <  1:3768451/150‑1 (MQ=255)
                                                                                       tGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTGTTGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAag                                                                <  1:1614532/150‑1 (MQ=255)
                                                                                         ttttCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGGAAGGAagag                                                              >  2:693958/1‑150 (MQ=255)
                                                                                          tttCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAagaga                                                             <  1:1317623/150‑1 (MQ=255)
                                                                                           ttCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAagagag                                                            >  1:2176256/1‑150 (MQ=255)
                                                                                            tCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGt                                                           <  2:4887219/150‑1 (MQ=255)
                                                                                             cATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTa                                                          >  1:146697/1‑150 (MQ=255)
                                                                                             cATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTa                                                          >  2:5108734/1‑150 (MQ=255)
                                                                                              aTTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTAt                                                         <  1:5471452/150‑1 (MQ=255)
                                                                                               gtGGAATACTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTTTTGTTTATGGGCTTGCTTCTGGAACAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATg                                                        <  2:3713506/149‑1 (MQ=255)
                                                                                                  gAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCtt                                                     <  1:5348637/150‑1 (MQ=255)
                                                                                                   aaTATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTa                                                    >  2:1322580/1‑150 (MQ=255)
                                                                                                      atTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACAt                                                 <  2:5843793/150‑1 (MQ=255)
                                                                                                      atTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACAt                                                 <  1:5132129/150‑1 (MQ=255)
                                                                                                       ttGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACAtt                                                <  2:495241/150‑1 (MQ=255)
                                                                                                       ttGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACAtt                                                <  1:4680319/150‑1 (MQ=255)
                                                                                                          agtttttttGGTTTAAACCAGACGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTAGAGCACCCGTATATATAGCTGGCATCTGAAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAg                                             <  1:3811585/147‑1 (MQ=255)
                                                                                                          aGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAg                                             >  2:2935173/1‑150 (MQ=255)
                                                                                                            cTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGaa                                           <  2:337080/150‑1 (MQ=255)
                                                                                                             ttttttGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGaaa                                          <  1:4077665/150‑1 (MQ=255)
                                                                                                             ttttttGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGaaa                                          <  1:3926793/150‑1 (MQ=255)
                                                                                                             ttttttGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGGTTACATTAAGaaa                                          >  2:4221006/1‑150 (MQ=255)
                                                                                                                tttGGTTTAAACCAGTCGATTTTTTTATCTTTTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAAc                                       <  2:2414727/150‑1 (MQ=255)
                                                                                                                 ttGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACt                                      <  1:664940/150‑1 (MQ=255)
                                                                                                                 ttGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACt                                      >  1:5219537/1‑150 (MQ=255)
                                                                                                                  tGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACtt                                     >  2:79481/1‑150 (MQ=255)
                                                                                                                   ggTTTAAACCAGTCGATTTTTTTATGTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTa                                    <  1:2284724/150‑1 (MQ=255)
                                                                                                                           ccAGTCGATTTTTTTATCTATTGTTTATGGGCTTCCTTCTGGAGCAGTCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGc                            <  1:829289/150‑1 (MQ=255)
                                                                                                                            cAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAATTTAGAGGAAGCa                           <  1:3057533/150‑1 (MQ=255)
                                                                                                                            cAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCa                           <  2:4685551/150‑1 (MQ=255)
                                                                                                                             aGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCaa                          >  2:4555221/1‑150 (MQ=255)
                                                                                                                              gTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAg                         <  2:5241833/150‑1 (MQ=255)
                                                                                                                                cGATTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTATGAAAATCTTAGAGGAAGCAAGGc                       >  1:4232675/1‑150 (MQ=255)
                                                                                                                                cGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGc                       >  2:4143673/1‑150 (MQ=255)
                                                                                                                                cGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGc                       <  2:3427924/150‑1 (MQ=255)
                                                                                                                                 ggtttttttATCTATTGTTTATGGGCTTGCTTCTGGTGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCa                      >  2:4475805/3‑150 (MQ=255)
                                                                                                                                 gATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCa                      >  1:955283/1‑150 (MQ=255)
                                                                                                                                 gATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCa                      <  2:5570162/150‑1 (MQ=255)
                                                                                                                                  aTTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTGCATTAAGAAAAACTTAAAGGAAGCAAGGCAg                     >  1:2901367/1‑150 (MQ=255)
                                                                                                                                  aTTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAg                     >  1:1717996/1‑150 (MQ=255)
                                                                                                                                   tttttttATCTATTGTTTATGGGCTTGCTTCTGGAGTAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGa                    >  1:3309881/1‑150 (MQ=255)
                                                                                                                                     tttttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGaaa                  <  2:1132249/150‑1 (MQ=255)
                                                                                                                                      ttttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGaaaa                 >  1:1133880/1‑150 (MQ=255)
                                                                                                                                      ttttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGaaaa                 >  2:1901950/1‑150 (MQ=255)
                                                                                                                                        ttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATg               >  2:2270062/1‑150 (MQ=255)
                                                                                                                                        ttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATg               >  1:2769966/1‑150 (MQ=255)
                                                                                                                                         tATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATgg              >  2:4581928/1‑150 (MQ=255)
                                                                                                                                         tATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATgg              >  1:4335357/1‑150 (MQ=255)
                                                                                                                                            cTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTcc           <  2:173944/150‑1 (MQ=255)
                                                                                                                                             tATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCg          >  2:2967049/1‑150 (MQ=255)
                                                                                                                                             tATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCg          >  2:2136013/1‑150 (MQ=255)
                                                                                                                                                tGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCGTCt       <  1:4851614/150‑1 (MQ=255)
                                                                                                                                                 gTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCGTCtt      <  2:115732/150‑1 (MQ=255)
                                                                                                                                                  tttATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCGTCTTa     <  1:2507386/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAACTGAGTATAGACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGATTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAAAATGGTCCGTCTTAGGA  >  CP006881/35811‑36109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑