Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,438,133 T→A 8.7% I439K (ATA→AAA)  U712_17650 → putative MFS‑type transporter yvkA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,438,1330TA 8.7% 1062.2 / 15.4 374I439K (ATA→AAA) U712_17650putative MFS‑type transporter yvkA
Reads supporting (aligned to +/- strand):  ref base T (212/113);  new base A (3/46);  total (215/159)
Fisher's exact test for biased strand distribution p-value = 5.63e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

CTCGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCTTCATAAG  >  CP006881/3437984‑3438282
                                                                                                                                                     |                                                                                                                                                     
ctcGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGAt                                                                                                                                                       <  1:1057957/150‑1 (MQ=255)
ctcGACCTTCCGTTATTTCGCAAGCATCATTTCATCAGCCTTAATTCGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTGTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGAt                                                                                                                                                       >  2:3150914/1‑150 (MQ=255)
  cGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAg                                                                                                                                                     <  1:2149508/150‑1 (MQ=255)
  cGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAg                                                                                                                                                     <  1:3657560/150‑1 (MQ=255)
  cGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAg                                                                                                                                                     <  1:991371/150‑1 (MQ=255)
   gACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGa                                                                                                                                                    >  1:1589293/1‑150 (MQ=255)
   gACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGa                                                                                                                                                    <  2:3613935/150‑1 (MQ=255)
       ttCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGaaaaa                                                                                                                                                <  2:5281044/150‑1 (MQ=255)
           gTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACt                                                                                                                                            <  2:1878950/150‑1 (MQ=255)
           gTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACt                                                                                                                                            >  2:2516274/1‑150 (MQ=255)
           gTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACt                                                                                                                                            >  1:3798403/1‑150 (MQ=255)
            ttATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTc                                                                                                                                           <  2:4202022/150‑1 (MQ=255)
             tATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCa                                                                                                                                          <  2:4977056/150‑1 (MQ=255)
                 tCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGt                                                                                                                                      <  1:2177094/150‑1 (MQ=255)
                  cGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTa                                                                                                                                     <  1:884904/150‑1 (MQ=255)
                  cGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAAACTGATGAAACGGCACGGATAGAAAAAAACTCAGCgaa                                                                                                                                     >  1:5040815/1‑148 (MQ=255)
                     aaGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAaca                                                                                                                                  <  1:1739546/150‑1 (MQ=255)
                     aaGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAaca                                                                                                                                  >  2:3417363/1‑150 (MQ=255)
                           catTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAAc                                                                                                                            <  2:1673517/150‑1 (MQ=255)
                             tttCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAg                                                                                                                          <  2:401615/150‑1 (MQ=255)
                              ttCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAgg                                                                                                                         >  2:742186/1‑149 (MQ=255)
                                catcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGccc                                                                                                                       <  2:3494224/150‑1 (MQ=255)
                                 atcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAAc                                                                                                                            >  1:4216853/1‑144 (MQ=255)
                                 atcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCg                                                                                                                      >  2:4714308/1‑150 (MQ=255)
                                 atcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCg                                                                                                                      <  2:126367/150‑1 (MQ=255)
                                 atcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCg                                                                                                                      >  2:2067405/1‑150 (MQ=255)
                                 atcaGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCg                                                                                                                      >  1:572047/1‑150 (MQ=255)
                                             tGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGcatcat                                                                                                          >  2:1194151/1‑150 (MQ=255)
                                              ggCCTGATTTCGGGATATCACACCCTATTTATGGTTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACCCAGCGTAACACAAAACAGCCCGGCATCGCatcatc                                                                                                         <  2:2917189/150‑1 (MQ=255)
                                               gCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGAAAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCTTCATcc                                                                                                        >  2:1110953/1‑150 (MQ=255)
                                                ccTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCt                                                                                                       <  1:3885562/150‑1 (MQ=255)
                                                ccTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCt                                                                                                       <  2:395367/150‑1 (MQ=255)
                                                ccTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCt                                                                                                       <  1:633532/150‑1 (MQ=255)
                                                 cTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTg                                                                                                      <  1:4918722/150‑1 (MQ=255)
                                                   gATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCatcatc                                                                                                         <  2:5714496/145‑1 (MQ=255)
                                                    aTTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCgg                                                                                                   <  1:2216712/150‑1 (MQ=255)
                                                     tttCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGt                                                                                                  <  2:2183732/150‑1 (MQ=255)
                                                      ttCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTGTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGATCGGAAATGCCTCATCCTGCGGTg                                                                                                 >  1:4450753/1‑150 (MQ=255)
                                                       tCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATTGGCGTTTTTGTTCCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGc                                                                                                <  2:3012535/150‑1 (MQ=255)
                                                        cGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGcc                                                                                               <  2:5505566/150‑1 (MQ=255)
                                                        cGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGcc                                                                                               <  1:2099600/150‑1 (MQ=255)
                                                         gggATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCg                                                                                              <  1:5221866/150‑1 (MQ=255)
                                                          ggATATCACACCCTATTTATGATTCTCTTTGCTGTATCCACCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCgg                                                                                             <  2:2581990/150‑1 (MQ=255)
                                                           gATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCAACCTGCGGTGCCggg                                                                                            >  1:2280292/1‑150 (MQ=255)
                                                           gATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCggg                                                                                            >  2:282157/1‑150 (MQ=255)
                                                             tatCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCAACAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCTTCCTGCGGTGCCGGGCt                                                                                          <  1:4170711/150‑1 (MQ=255)
                                                               tCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCATCCTGCGGTGCCGGGCTGc                                                                                        >  1:527414/1‑150 (MQ=255)
                                                                 acacCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGGGCCGGGCTGCCt                                                                                      >  2:156941/1‑150 (MQ=255)
                                                                 acacCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACCGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCCGGCTGCCt                                                                                      >  1:2886436/1‑150 (MQ=255)
                                                                  cacCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTGTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACTGCACGGCCGCGCCTTATCCCGCGGTACCtggttcgcgt                                                                                     >  1:827134/1‑143 (MQ=255)
                                                                   cccttttttATGTTTCTTTTTGCTTTACCCACCATCGGCTTTTTTTTTTCATTGGGCAACAAATCTGATAAAACGGCAGGAAAAAAAAAAAACCCAGCGTAACACAAACCCCCCCGCCACCGCTTCATCCGGCGGTGCCGGGCTGCCttt                                                                                    <  2:1774292/144‑1 (MQ=255)
                                                                    ccttttttATGTTTCTTTTTGCTGTATCCATCATCGGCTTTTTTGTTCCATTGGGCATCAAATCTGATGAAACGGCACGGAAAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGCCTGCCtttt                                                                                   <  2:1998882/145‑1 (MQ=255)
                                                                    cccTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCtttt                                                                                   >  2:3846242/1‑150 (MQ=255)
                                                                       tATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCCGGCTGCCTTTTATg                                                                                >  1:2490027/1‑150 (MQ=255)
                                                                              gATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGa                                                                         >  1:3005410/1‑150 (MQ=255)
                                                                               aTTCTTTTTTCTGTATCCACCATGGGCGTTTTTGTTTCATTGGGCAACAAATCTGATGAAACGGCACGAAAAGAAAAAAACTCAGCGTAACACAACACAGCCGGGCTCCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGaa                                                                        <  1:5807811/150‑1 (MQ=255)
                                                                               aTTCTCTTTGCTGTATCCATCATCGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGTCCGGCATCGCATCATCCTGCGATGCCGGGCTGCCTTTTATGCTCCCGaa                                                                        >  2:2138623/1‑150 (MQ=255)
                                                                                 tctcTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGc                                                                      >  2:5597175/1‑150 (MQ=255)
                                                                                    ctTTGCTGTATCCATCATCGGCGTATTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGAACGGCATCGCATCAACCTGCGGTGCCGGGCTGCCTTTTATGATCCCTAAGCGAc                                                                   >  1:4588568/1‑150 (MQ=255)
                                                                                        gCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCt                                                               >  1:4668861/1‑150 (MQ=255)
                                                                                        gCTGTACCCACCATCGGCTTTTTTGTTTCATAGGGAATCAAATCTGATGAAACGGCACGGAAAGAAAAAACCTCAGCGTAACACAAAACAGCCCGGCATCCCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCt                                                               <  2:3726041/150‑1 (MQ=255)
                                                                                            tATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGGTCCCGAAGCGACTGCTTCGt                                                           >  2:1349030/1‑150 (MQ=255)
                                                                                              tCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCTCGAAAAAAAAAAAACTCAGCGTACCACAAACCAGCCCGGCATCCCATCATCTTGCGGTGCCGTACTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCt                                                         <  1:5691668/150‑1 (MQ=255)
                                                                                                 atcatcGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCATAACACAAAACAGATCGTCATAGCATCAACCTGCTGTGGCGGGCTGCCTTTTATGATCCCGAAACGACTGGTTCGTCTTCa                                                      >  1:4263446/1‑150 (MQ=255)
                                                                                                   caccGGGGTTTTTGTTTCATTGGGCATCAAATCTGATAAAACGGCACGGAAAAAAAAAAACACAGCGTAACACAAACCACCCCGGCATCGCACCATCCTGCGGGGCCGGCCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACg                                                    <  2:4937689/147‑1 (MQ=255)
                                                                                                    atcGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCCGGGCCGGGCTGGCTTTTATGATCCCCAAGGGACTGCTTTCTCTTCACgg                                                   >  1:5571672/1‑150 (MQ=255)
                                                                                                    atcGGCGTCTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCCGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACgg                                                   >  2:2321752/1‑150 (MQ=255)
                                                                                                    atcGGCGTATTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGGTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACgg                                                   >  1:3238355/1‑150 (MQ=255)
                                                                                                     tcGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCCACTGGCTGGTCTTTAAGGt                                                  >  1:1225291/1‑150 (MQ=255)
                                                                                                        gCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAACGACTGCTTCGTCTTCACGGTCAt                                               >  1:2648610/1‑150 (MQ=255)
                                                                                                         cGTTTTTGTTTCATTGGACAACAAATCTGATGAAACGGCACGGAAAGAAAAAAACTCAGCGAAACACAAAACAGCCCGGCACCGCACCATCCTGGGGGGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATc                                              <  2:136341/150‑1 (MQ=255)
                                                                                                          ttttttGTTTCATTGGGCACCAAATCTGATGAAACGGCACGGAAAAAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCACCACCCTGCGGTGCCGGTCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATcc                                             <  2:5079257/149‑1 (MQ=255)
                                                                                                          gTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCAACCTGCGGTGCCGGGCTGCCTTTTATGATCCCGTAGTGAATGCTTCGTTTTTACGGTTATcc                                             >  1:4066782/1‑150 (MQ=255)
                                                                                                           ttttttttTCATTGGGCATAAAATCTAATAAAAGGGAAGGAATAAAAAAAAACTCAGCTTAAAACAAACAAGCCCGGATTCGCAACATCTTGGGTTGCCGTTCTTCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCt                                            <  1:3575447/150‑1 (MQ=255)
                                                                                                            ttttttttGATTGGGCACAAAATCTGAAGAAACGGCAGGGAAAAAAAAAAACGCACCGTAACACAAAACAGCCCGCCATCCCTCCATCCTGCGGTGCCGGTCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCtt                                           <  2:4869189/150‑1 (MQ=255)
                                                                                                            ttttGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCCTCGCATCATCCTGCGGTGTCGGGCCGGCTTTTATGGTCCCCTAGAGACTGCTTCGTCTTCACGGACATCCtt                                           >  2:2679715/1‑150 (MQ=255)
                                                                                                             tttGTTTCATGGGGCAACAAACGTGATGAAACGACACGGAAAAAAAAAAACTCAGCGTAACACAAACCAGCCCGGCATCGCATCATCTTGGGGTCCCGGTCTTCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCttt                                          <  2:4345060/150‑1 (MQ=255)
                                                                                                              ttGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCtttt                                         >  1:849065/1‑149 (MQ=255)
                                                                                                               tGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTAc                                        >  2:555121/1‑150 (MQ=255)
                                                                                                                gTTTTAGTGGACATAAATTGTGAAAAAACGGCACGAATAAAAAAAAACCCAGCAAAACAAAAAACAGGCCGGCATCGTATCATCCTGCGGTCCCGGTCTACCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACg                                       <  1:4335892/150‑1 (MQ=255)
                                                                                                                   tCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAag                                    >  1:1406161/1‑150 (MQ=255)
                                                                                                                       tGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGa                                >  2:3241419/1‑150 (MQ=255)
                                                                                                                       tGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGa                                >  2:66860/1‑150 (MQ=255)
                                                                                                                       tGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGa                                >  1:1945295/1‑150 (MQ=255)
                                                                                                                            aTCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGccc                           >  2:4236711/1‑150 (MQ=255)
                                                                                                                            aTCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCAGCGCATCATCCTTCGGTGCCGGGGTGCCTTTTATGATCCCGAAGCGAATGGTTCGTTTTCACGGTCATCCTTTACCAAGAGGATGccc                           >  1:3113714/1‑150 (MQ=255)
                                                                                                                            aTCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGACCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGccc                           >  2:4636201/1‑150 (MQ=255)
                                                                                                                             tCAAACCTGAGGAAAGGGCAGGAAAAAAAAAAAACTCACCTAAACCCAAAACAGCCCGGCATCCCACCACCCTGCGGTGCCGGTCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCa                          <  2:4450753/150‑1 (MQ=255)
                                                                                                                              cAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCaa                         >  1:4012110/1‑150 (MQ=255)
                                                                                                                                aatcaaatgaAAACGCACAGATAGAACAAAACCCAGTGTAACACAAAACAGCCCGCCAGCACATCATCCTGGGGTGCGGTGCTGCCTTTTATGATCCCGAGGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAAtt                       <  1:203952/144‑1 (MQ=255)
                                                                                                                                   ttgatgaACCGGCAGGGATAGAAAAAACCTCACCGTAACACAAAACACCCCGGCACCGCATCATCCTGCGGTGCTGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCat                    <  2:1426451/149‑1 (MQ=255)
                                                                                                                                   cTGATAAAACGGCACGGAAAGAAAAAAACCCAGCGTAACACAAAACACCCGGGCATCGCATCATCCTGCGGTGCCGGCCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCat                    <  2:4134140/150‑1 (MQ=255)
                                                                                                                                    tgatgaAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGGCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCatg                   >  1:472949/1‑150 (MQ=255)
                                                                                                                                    tgataaAGCGGCAGGGAAAAAAAAAAACTAAGTATAACACAAAACAGCCCGACAGGCAATCACCTTGCGGTGCAGGCCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCatg                   <  1:350502/150‑1 (MQ=255)
                                                                                                                                     gatgaAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCatga                  >  1:769016/1‑150 (MQ=255)
                                                                                                                                               cACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCtt        >  1:4854847/1‑150 (MQ=255)
                                                                                                                                                 cGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCTTCa      >  1:1117389/1‑150 (MQ=255)
                                                                                                                                                  ggATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCTTCAt     >  1:148668/1‑150 (MQ=255)
                                                                                                                                                   gttAGAAAAAAACTCATCGTAACACAACACCGCCCGGCACAGCATCATCCTGCGGGGGGGGGCCGCCGTTTATGATCCCGAAATGACCGCTTCGTCTTCACGGTCATCCTTTGCGTCGAGGGTGCCCAATACATGACGTTCTCCTCCAtc    >  2:1302873/3‑149 (MQ=255)
                                                                                                                                                   gATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCACCATCCTGGGGTGCCGGTCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCTTCATa    <  2:522815/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCGACCTTCCGTTATTTCGGAAGCATCATTTCATCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGTTTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAACACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACGGTCATCCTTTACGAAGAGGATGCCCAATTCATGATGTTCTCCTTCATAAG  >  CP006881/3437984‑3438282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑