Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,252 T→A 100% S22S (TCT→TCA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,2520TA99.8% 1037.6 / ‑6.4 289S22S (TCT→TCA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (143/144);  total (144/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 289 total aligned reads displayed.

AAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGC  >  CP006881/3723105‑3723398
                                                                                                                                                   |                                                                                                                                                  
aaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAg                                                                                                                                                  >  2:680064/1‑150 (MQ=255)
 aaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGc                                                                                                                                                 >  1:2556620/1‑150 (MQ=255)
  aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCa                                                                                                                                                <  1:4169435/150‑1 (MQ=255)
   ttCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaa                                                                                                                                               <  2:4403244/150‑1 (MQ=255)
     cGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa                                                                                                                                             >  2:3656849/1‑150 (MQ=255)
     cGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa                                                                                                                                             >  2:4426412/1‑150 (MQ=255)
     cGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa                                                                                                                                             <  2:4998275/150‑1 (MQ=255)
      gTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAt                                                                                                                                            >  2:2173230/1‑150 (MQ=255)
        aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc                                                                                                                                          >  1:1344851/1‑150 (MQ=255)
          aaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCACAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGt                                                                                                                                        <  2:2763747/150‑1 (MQ=255)
           aattaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAAAAAGCAGGAAAAGACAATGAAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt                                                                                                                                       >  1:2235982/7‑150 (MQ=255)
             ttaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGGTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAt                                                                                                                                     >  1:410776/5‑150 (MQ=255)
             ttaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGGAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAt                                                                                                                                     >  2:2663712/5‑150 (MQ=255)
             ttaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAt                                                                                                                                     >  2:762065/5‑150 (MQ=255)
               aattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAtgt                                                                                                                                   <  2:375147/148‑1 (MQ=255)
                 ttGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGc                                                                                                                                 <  1:484079/150‑1 (MQ=255)
                     gAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcg                                                                                                                             >  1:3980505/1‑147 (MQ=255)
                     gAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcg                                                                                                                             >  1:871479/1‑147 (MQ=255)
                      aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcgg                                                                                                                            <  1:1024465/150‑5 (MQ=255)
                              cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAtttt                                                                                                                    <  1:2859210/150‑1 (MQ=255)
                              cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAtttt                                                                                                                    <  1:680064/150‑1 (MQ=255)
                               acaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTg                                                                                                                   >  1:4682110/1‑150 (MQ=255)
                                  atatCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACA‑‑‑‑‑‑‑‑‑GAA‑AAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                                                                                                      <  1:5444703/150‑1 (MQ=255)
                                   tatCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCa                                                                                                               <  2:4462913/150‑1 (MQ=255)
                                    atCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAg                                                                                                              >  1:636829/1‑150 (MQ=255)
                                     tCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgt                                                                                                             <  2:3106668/150‑2 (MQ=255)
                                         gCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTAtat                                                                                                         <  2:2247615/150‑3 (MQ=255)
                                             taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAt                                                                                                     <  2:866130/150‑1 (MQ=255)
                                             taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAt                                                                                                     <  2:2725011/150‑1 (MQ=255)
                                               atATGCTCAATCATGAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATAttttttg                                                                                                   >  2:1600294/2‑146 (MQ=255)
                                                 aTGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggt                                                                                                 <  1:979338/150‑2 (MQ=255)
                                                 aTGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggt                                                                                                 >  1:1744235/1‑149 (MQ=255)
                                                    cTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGtttt                                                                                              >  2:4885512/1‑149 (MQ=255)
                                                     tCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGttttg                                                                                             <  2:1495962/150‑3 (MQ=255)
                                                      cAATCANGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTgg                                                                                            <  1:3194297/150‑1 (MQ=255)
                                                         tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCAt                                                                                         <  2:189340/149‑1 (MQ=255)
                                                          cATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCAtt                                                                                        <  1:1678854/150‑1 (MQ=255)
                                                            tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTAc                                                                                      >  1:769141/3‑150 (MQ=255)
                                                             gaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACa                                                                                     >  1:3391454/2‑150 (MQ=255)
                                                             gaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACa                                                                                     <  1:2121618/149‑1 (MQ=255)
                                                              aaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAg                                                                                    <  1:1194742/150‑1 (MQ=255)
                                                              aaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAg                                                                                    <  2:2006871/150‑1 (MQ=255)
                                                                agaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGcc                                                                                  <  2:3170473/150‑1 (MQ=255)
                                                                agaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGcc                                                                                  >  1:4958650/1‑150 (MQ=255)
                                                                 gaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTTTGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCg                                                                                 >  1:2110088/1‑150 (MQ=255)
                                                                  aaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCgc                                                                                <  2:4783263/150‑1 (MQ=255)
                                                                       aGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCgcaagca                                                                           <  2:3595519/150‑1 (MQ=255)
                                                                        ggAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCgcaagcaa                                                                          <  1:518301/150‑1 (MQ=255)
                                                                         gAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCaaa                                                                         <  1:749762/150‑1 (MQ=255)
                                                                          aaaagaCAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCaaaa                                                                        >  2:5308335/1‑150 (MQ=255)
                                                                           aaagaCAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAAc                                                                       <  2:4103498/150‑1 (MQ=255)
                                                                           aaagaCAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAAc                                                                       <  1:4383318/150‑1 (MQ=255)
                                                                              gaCATTGGAAACTCAAGCTTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAgg                                                                    <  2:922662/149‑1 (MQ=255)
                                                                               aCAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGc                                                                   >  2:583349/1‑150 (MQ=255)
                                                                                 aaTGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAg                                                                 >  1:4508672/1‑150 (MQ=255)
                                                                                  aTGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAgc                                                                <  2:3612119/150‑2 (MQ=255)
                                                                                  aTGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAgc                                                                <  2:5558073/150‑2 (MQ=255)
                                                                                     gAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                             <  2:1157004/150‑1 (MQ=255)
                                                                                     gAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAt                                                             >  1:83097/1‑150 (MQ=255)
                                                                                      aaaCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAACCAGGCAGCTAtt                                                            <  1:1469370/150‑1 (MQ=255)
                                                                                      aaaCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAtt                                                            >  1:2348346/1‑150 (MQ=255)
                                                                                      aaaCACAAGCATTACAAAAGGTTAAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAtt                                                            <  2:3342063/150‑1 (MQ=255)
                                                                                       aaCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAttt                                                           >  1:2989207/1‑150 (MQ=255)
                                                                                        aCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtc                                                          >  1:3716208/1‑150 (MQ=255)
                                                                                         cTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGGTATTtct                                                         >  1:4878438/1‑150 (MQ=255)
                                                                                         cTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtct                                                         >  2:3265679/1‑150 (MQ=255)
                                                                                          tCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtctt                                                        <  2:3521546/150‑2 (MQ=255)
                                                                                          tCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtctt                                                        <  2:4013682/150‑2 (MQ=255)
                                                                                          tCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtctt                                                        <  2:2983830/150‑2 (MQ=255)
                                                                                          tCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtctt                                                        <  1:1891409/150‑2 (MQ=255)
                                                                                             aGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTAt                                                     >  2:5144168/1‑150 (MQ=255)
                                                                                               cATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATgg                                                   >  2:38695/1‑150 (MQ=255)
                                                                                               cATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATgg                                                   >  2:2466864/1‑150 (MQ=255)
                                                                                                aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                  >  2:4668810/1‑150 (MQ=255)
                                                                                                    cAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGAt                                              >  2:1804285/1‑150 (MQ=255)
                                                                                                      aaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtc                                            <  1:4219418/150‑1 (MQ=255)
                                                                                                        aggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtctc                                          <  2:5750084/148‑1 (MQ=255)
                                                                                                          gTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCg                                        >  2:1692191/1‑150 (MQ=255)
                                                                                                            tGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGtt                                      <  2:565367/150‑1 (MQ=255)
                                                                                                            tGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGtt                                      <  2:5431734/150‑1 (MQ=255)
                                                                                                              aaCAGTATGCTTTGACAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTCATTGGTTTTGGCATGACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTg                                    >  2:1394360/1‑150 (MQ=255)
                                                                                                              aaCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTg                                    >  2:828467/1‑150 (MQ=255)
                                                                                                              aaCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTg                                    <  1:2173230/150‑1 (MQ=255)
                                                                                                               aCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGc                                   <  2:3062063/150‑1 (MQ=255)
                                                                                                                cAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGcc                                  >  1:204121/1‑149 (MQ=255)
                                                                                                                cAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGcc                                  >  1:5762811/1‑149 (MQ=255)
                                                                                                                     tgCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTcc                             <  2:2556620/149‑1 (MQ=255)
                                                                                                                      gCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCg                            >  1:887321/1‑150 (MQ=255)
                                                                                                                          tGAAAAAACAAATCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAg                        <  1:1120715/150‑1 (MQ=255)
                                                                                                                          tGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAg                        <  2:4303108/150‑1 (MQ=255)
                                                                                                                              aaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGc                    >  2:3031329/2‑150 (MQ=255)
                                                                                                                              aaaacaaaacaTATTCGCTTCAAGCAAAATCCGATATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTAGTCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTTCCTTTCCGGCAGCCGc                    >  2:1635825/2‑150 (MQ=255)
                                                                                                                                 acaaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGt                 >  1:2759171/4‑150 (MQ=255)
                                                                                                                                  caaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTc                >  1:5643775/3‑150 (MQ=255)
                                                                                                                                   aaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGGCGCTGTca               >  1:5504787/2‑150 (MQ=255)
                                                                                                                                   aaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTca               >  1:4031160/2‑150 (MQ=255)
                                                                                                                                         tatTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCg         <  1:5363335/150‑1 (MQ=255)
                                                                                                                                          attcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg        <  2:3716208/144‑1 (MQ=255)
                                                                                                                                             cgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGc     >  1:3152500/4‑150 (MQ=255)
                                                                                                                                               cttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCAAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg   <  1:3627906/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGC  >  CP006881/3723105‑3723398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑