Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,725,239 2 bp→AA 100% intergenic (‑71/‑457) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,2390GA100.0% 1462.3 / ‑6.6 420intergenic (‑71/‑458)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (230/187);  minor base C (2/0);  total (232/188)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*CP0068813,725,2400GA100.0% 1482.9 / ‑6.6 420intergenic (‑72/‑457)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (232/187);  minor base C (0/1);  total (232/188)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 420 total aligned reads displayed.

TTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCT  >  CP006881/3725098‑3725380
                                                                                                                                               ||                                                                                                                                            
tttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAg                                                                                                                                        <  2:2688273/150‑1 (MQ=255)
 ttCTGTCCGGCGTCACCCAGTTCATCAAAAAGAGCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGNCGCAGCCCGCAAGAAAAGa                                                                                                                                       >  2:5699496/1‑150 (MQ=255)
  tCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGAc                                                                                                                                      >  2:2511704/1‑150 (MQ=255)
  tCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGAc                                                                                                                                      <  2:2827758/150‑1 (MQ=255)
   cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACg                                                                                                                                     >  1:2242232/1‑150 (MQ=255)
     gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc                                                                                                                                   >  1:3803081/1‑150 (MQ=255)
         ggCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAt                                                                                                                               >  2:295767/1‑150 (MQ=255)
           cGTCACCCAGTTCATCAAAAAGAGCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCTCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAttt                                                                                                                             <  1:3582749/150‑1 (MQ=255)
            gTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtatc                                                                                                                            >  2:3679194/1‑150 (MQ=255)
            gTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttc                                                                                                                            >  2:3140297/1‑150 (MQ=255)
             tCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttct                                                                                                                           >  1:5564740/1‑150 (MQ=255)
             tCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttct                                                                                                                           <  1:4182630/150‑1 (MQ=255)
                   aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAACCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTtctc                                                                                                                     <  2:2449723/150‑1 (MQ=255)
                   aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCGTAGAAAAACATCCCTCCGCATCTTTTTGGCAGGTAGCCTTT‑TTATCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCCTTtctc                                                                                                                     <  1:4036717/150‑1 (MQ=255)
                   aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGGCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTtctc                                                                                                                     >  1:5017710/1‑150 (MQ=255)
                   aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTtctc                                                                                                                     <  2:3337597/150‑1 (MQ=255)
                      ccatcaACAAGACGATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAACACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTCTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGTCGACCGATTTCTTTTCTCGCa                                                                                                                  <  2:3572997/149‑1 (MQ=255)
                        atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCaaa                                                                                                                >  2:822045/1‑150 (MQ=255)
                          caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACt                                                                                                              >  1:4489679/1‑150 (MQ=255)
                           aaaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTa                                                                                                             <  2:498259/150‑1 (MQ=255)
                             aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGc                                                                                                           <  2:4864004/150‑1 (MQ=255)
                               aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCgg                                                                                                         >  2:2170066/1‑150 (MQ=255)
                                gACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc                                                                                                        >  1:1473559/1‑150 (MQ=255)
                                     tCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAg                                                                                                   <  2:5604000/150‑1 (MQ=255)
                                        acacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa                                                                                                <  2:5063747/150‑1 (MQ=255)
                                         tacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATGCCTACTCTTCTTTTTGACAGTCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGTCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTACCGGCTCTAGCaaa                                                                                               <  2:1066216/149‑1 (MQ=255)
                                         cacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGTCGCAGCCCACAAGAAAAAACGGACGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaa                                                                                               >  1:5218253/1‑150 (MQ=255)
                                         cacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaa                                                                                               >  2:2308066/1‑150 (MQ=255)
                                          acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                                                              >  1:2317221/1‑150 (MQ=255)
                                             cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGTTCTAGCAAAACTc                                                                                           >  1:2663866/1‑150 (MQ=255)
                                             cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTc                                                                                           >  2:5364888/1‑150 (MQ=255)
                                               tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                                                                         <  1:908655/150‑1 (MQ=255)
                                                aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg                                                                                        >  2:3912063/1‑150 (MQ=255)
                                                aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg                                                                                        >  1:1291667/1‑150 (MQ=255)
                                                  aGATACAAGCATACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAc                                                                                      <  1:4426819/150‑1 (MQ=255)
                                                        aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                                                                <  2:3082586/150‑1 (MQ=255)
                                                         aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa                                                                               >  2:1586564/1‑150 (MQ=255)
                                                            aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAg                                                                            >  1:5659679/1‑150 (MQ=255)
                                                              aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc                                                                          <  1:2895944/150‑1 (MQ=255)
                                                               cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAcc                                                                         <  1:122336/150‑1 (MQ=255)
                                                               cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAcc                                                                         >  2:2024785/1‑150 (MQ=255)
                                                                  ttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTg                                                                      <  2:1747641/150‑1 (MQ=255)
                                                                   tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg                                                                     <  2:5243455/150‑1 (MQ=255)
                                                                     aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                                                   >  2:419822/1‑150 (MQ=255)
                                                                     aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                                                   >  2:403024/1‑150 (MQ=255)
                                                                     aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                                                   >  1:4782926/1‑150 (MQ=255)
                                                                     aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                                                   >  2:345259/1‑150 (MQ=255)
                                                                        gAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTCCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGACTCTATGATAGACCGTGGAAaga                                                                <  2:3015843/150‑1 (MQ=255)
                                                                          aaaaCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAgaga                                                              >  2:4352616/1‑150 (MQ=255)
                                                                              cATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTc                                                          >  2:1876843/1‑150 (MQ=255)
                                                                               aTCCCTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                                                         <  2:1390230/150‑1 (MQ=255)
                                                                                 cccTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCttt                                                       >  2:4486848/1‑150 (MQ=255)
                                                                                   cTCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt                                                     >  2:3780456/1‑150 (MQ=255)
                                                                                    tCCGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTc                                                    <  2:463466/150‑1 (MQ=255)
                                                                                     ccGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTca                                                   >  2:1816762/1‑150 (MQ=255)
                                                                                      cGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcac                                                  >  1:1049234/1‑150 (MQ=255)
                                                                                      cGCTTCTTTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcac                                                  >  1:616516/1‑150 (MQ=255)
                                                                                        cttcttTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACaa                                                <  1:4538269/150‑1 (MQ=255)
                                                                                        cttcttTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACaa                                                >  1:1641353/1‑150 (MQ=255)
                                                                                         ttcttTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAat                                               <  1:213737/150‑1 (MQ=255)
                                                                                          tcttTTTGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAata                                              >  1:24709/1‑150 (MQ=255)
                                                                                             ttttGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCCTGGAAAGAGAATTCTTTGTCACAATATGa                                           >  1:1156626/1‑150 (MQ=255)
                                                                                             ttttGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAAAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGGTAGACCATGGAAAGAGAATTCTTTGTCACAATATGa                                           >  2:694676/1‑150 (MQ=255)
                                                                                               ttGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGt                                         <  2:1135766/150‑1 (MQ=255)
                                                                                               ttGGCAGGCAGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGt                                         <  2:481579/150‑1 (MQ=255)
                                                                                                 ggcaggcaGCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTTTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGa                                       >  1:3499330/1‑150 (MQ=255)
                                                                                                   caggcagCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaa                                     <  2:1251047/150‑1 (MQ=255)
                                                                                                   caggcagCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaa                                     >  1:2997070/1‑150 (MQ=255)
                                                                                                    aggcagCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaaa                                    <  1:5349505/150‑1 (MQ=255)
                                                                                                       cagCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAc                                 <  2:1517842/150‑1 (MQ=255)
                                                                                                       cagCCTTT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAc                                 <  2:1966589/150‑1 (MQ=255)
                                                                                                          ccttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa                              >  1:3701765/4‑150 (MQ=255)
                                                                                                           cttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                             >  2:2590900/3‑150 (MQ=255)
                                                                                                           cttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                             >  2:4531465/3‑150 (MQ=255)
                                                                                                           cttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                             >  1:2359364/3‑150 (MQ=255)
                                                                                                            ttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACt                            >  1:1378592/2‑150 (MQ=255)
                                                                                                            ttt‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACt                            <  1:5114722/149‑1 (MQ=255)
                                                                                                              t‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAg                          <  2:4429812/150‑1 (MQ=255)
                                                                                                              t‑ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGATCTAGCAAAACTCATGACTCTATGATAGACCCTGGGAAGAGAATTGTTTGTCACAATATGAGTGAAAAAACCAACCAg                          >  1:3270413/1‑150 (MQ=255)
                                                                                                                ttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGt                         <  2:1473559/150‑1 (MQ=255)
                                                                                                                 tAGCAGCCCGTTCTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGtt                        <  2:1005037/150‑1 (MQ=255)
                                                                                                                  agcagcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGCCTCTATGATAGGCCGTGGAAAAAGAATTCTTAGTCACAATATGTGTGAAAAGACCAACAAAttt                       >  2:5483421/1‑150 (MQ=255)
                                                                                                                  agcagcCCGTTCTCTCAGCGTCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAATCTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGttt                       <  2:211307/150‑1 (MQ=255)
                                                                                                                   gcagcCCGTTTTCTCAGCTGCAGCCGGCAAGAAAAGACGATCGATTTTTTTTCACGCAAACTAGCGTCTTTAGCAAAACACATGACTCTATGATAGAACGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGtttt                      <  2:1190694/150‑1 (MQ=255)
                                                                                                                     agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTa                    >  1:5775992/1‑150 (MQ=255)
                                                                                                                         cGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGaac                >  2:3863128/1‑149 (MQ=255)
                                                                                                                         cGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAg                >  1:4927583/1‑150 (MQ=255)
                                                                                                                            tttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGttt             <  1:3140297/150‑1 (MQ=255)
                                                                                                                            tttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGttt             <  2:5659679/150‑1 (MQ=255)
                                                                                                                            tttCTCAGCCACAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGttt             <  1:1668790/150‑1 (MQ=255)
                                                                                                                             ttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGtttt            <  1:1860713/150‑1 (MQ=255)
                                                                                                                               ctcAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTttgt          <  2:3230179/150‑1 (MQ=255)
                                                                                                                                tcAGCAGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTTGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTttgtt         <  2:5180030/150‑1 (MQ=255)
                                                                                                                                   gCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaa      <  1:1992816/150‑1 (MQ=255)
                                                                                                                                    ccGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaaa     >  2:492274/1‑150 (MQ=255)
                                                                                                                                    ccGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaaa     <  1:4303064/150‑1 (MQ=255)
                                                                                                                                     cGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAg    <  1:872385/150‑1 (MQ=255)
                                                                                                                                      gCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGc   <  2:90983/150‑1 (MQ=255)
                                                                                                                                       cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCt  <  1:766919/150‑1 (MQ=255)
                                                                                                                                       cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCt  <  2:1646591/150‑1 (MQ=255)
                                                                                                                                               ||                                                                                                                                            
TTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TT‑TTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCT  >  CP006881/3725098‑3725380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑